##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364472_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2548594 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.84163895857873 31.0 28.0 31.0 25.0 34.0 2 29.074900906146684 31.0 28.0 33.0 25.0 34.0 3 29.12533302675907 31.0 28.0 34.0 25.0 34.0 4 32.56606701577419 35.0 32.0 37.0 26.0 37.0 5 31.63988340237794 35.0 32.0 37.0 19.0 37.0 6 31.679645718384332 35.0 32.0 37.0 19.0 37.0 7 31.175247607112 35.0 31.0 37.0 17.0 37.0 8 31.270784204938096 35.0 32.0 37.0 17.0 37.0 9 31.866808522659944 37.0 30.0 39.0 11.0 39.0 10-11 32.04366368279922 37.0 30.5 39.0 14.0 39.0 12-13 31.786189169400856 37.0 30.5 39.0 11.5 39.0 14-15 32.72831412143323 38.0 31.0 40.0 10.5 41.0 16-17 32.784056228649995 38.0 31.0 40.0 11.0 41.0 18-19 32.64414810676004 38.0 31.0 40.0 10.0 41.0 20-21 32.536951158167994 38.0 30.5 40.0 10.0 41.0 22-23 32.498500741977736 38.0 30.5 40.0 10.0 41.0 24-25 32.29932582435649 37.0 30.0 40.0 10.0 41.0 26-27 32.38075895964599 37.0 30.0 40.0 10.0 41.0 28-29 32.27075928923948 37.0 30.0 40.0 9.0 41.0 30-31 31.778564965624184 37.0 30.0 40.0 8.0 41.0 32-33 31.71772338002836 37.0 30.0 40.0 7.5 41.0 34-35 31.52009970987925 37.0 30.0 40.0 2.0 41.0 36-37 31.50516578945097 37.0 30.0 40.0 2.0 41.0 38-39 31.27679202729034 37.0 30.0 40.0 2.0 41.0 40-41 31.012902211964715 36.0 29.0 40.0 2.0 41.0 42-43 30.895240866140313 36.0 28.5 40.0 2.0 41.0 44-45 30.819982900375656 36.0 29.0 40.0 2.0 41.0 46-47 30.692042357472396 36.0 28.5 40.0 2.0 41.0 48-49 30.44300681081412 36.0 28.0 40.0 2.0 41.0 50-51 30.10858712686289 36.0 27.0 40.0 2.0 41.0 52-53 30.01438224369986 36.0 27.0 40.0 2.0 41.0 54-55 29.55878319575421 35.0 26.0 40.0 2.0 41.0 56-57 29.56846677030551 35.0 26.0 39.5 2.0 41.0 58-59 29.33470866681786 35.0 26.0 39.0 2.0 41.0 60-61 29.05932035467399 35.0 26.0 39.0 2.0 41.0 62-63 28.79936349218432 34.5 26.0 39.0 2.0 41.0 64-65 28.332095853635376 34.0 24.0 38.5 2.0 40.0 66-67 27.94664764179779 34.0 23.0 38.0 2.0 40.0 68-69 27.76980484141452 34.0 22.5 37.5 2.0 40.0 70-71 27.83536000634075 34.0 24.5 37.0 2.0 40.0 72-73 27.608484521269375 34.0 24.5 37.0 2.0 39.0 74-75 27.23429820520648 34.0 22.5 36.0 2.0 39.0 76-77 27.14360035376368 34.0 24.0 36.0 2.0 39.0 78-79 26.748340653709455 33.0 22.5 35.5 2.0 37.5 80-81 26.507417619283416 33.0 22.0 35.0 2.0 37.0 82-83 26.359293006261492 33.0 22.0 35.0 2.0 37.0 84-85 25.933256729004306 33.0 20.0 35.0 2.0 36.0 86-87 25.791117180688644 33.0 20.0 35.0 2.0 36.0 88-89 25.746134142982363 33.0 20.0 35.0 2.0 36.0 90-91 25.578444428575125 33.0 20.0 35.0 2.0 35.0 92-93 25.583952171275612 33.0 20.0 35.0 2.0 35.0 94-95 25.592390353269295 33.0 20.0 35.0 2.0 35.0 96-97 25.47491813133045 33.0 20.0 35.0 2.0 35.0 98-99 25.333400298360587 33.0 19.5 35.0 2.0 35.0 100-101 25.24084769877038 33.0 18.5 35.0 2.0 35.0 102-103 24.118162602595785 30.5 17.5 34.0 2.0 35.0 104-105 25.0511087682071 32.0 19.0 35.0 2.0 35.0 106-107 25.150498274735014 33.0 18.0 35.0 2.0 35.0 108-109 25.220631846421988 33.0 18.5 35.0 2.0 35.0 110-111 25.15752195131904 33.0 18.0 35.0 2.0 35.0 112-113 25.108306187646996 33.0 18.0 35.0 2.0 35.0 114-115 25.05414495207946 33.0 17.0 35.0 2.0 35.0 116-117 24.97069776512069 33.0 14.0 35.0 2.0 35.0 118-119 24.84901125875679 33.0 6.5 35.0 2.0 35.0 120-121 24.686165783957744 33.0 2.0 35.0 2.0 35.0 122-123 24.391664776735723 33.0 2.0 35.0 2.0 35.0 124-125 23.622172460580224 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0810827099108558 1101 2 1.1095506527875614 1101 3 1.0458655149652643 1101 4 1.0437039255553842 1101 5 1.239562260649624 1101 6 1.3053201737446756 1101 7 1.6801667453135956 1101 8 1.552441613309405 1101 9 2.2570855553785165 1101 10-11 1.8769882255419326 1101 12-13 2.0497141461365693 1101 14-15 2.041134863230525 1101 16-17 2.0462580686253062 1101 18-19 2.002281569521571 1101 20-21 2.2602473638524003 1101 22-23 2.250964185565593 1101 24-25 2.203386676745758 1101 26-27 2.0110499616903894 1101 28-29 2.0573777212098108 1101 30-31 2.579852540850723 1101 32-33 2.3831993518654357 1101 34-35 2.414119802695904 1101 36-37 2.20904943846158 1101 38-39 2.367342188094721 1101 40-41 2.602455585869926 1101 42-43 2.429315554775009 1101 44-45 2.3313604744430556 1101 46-47 2.142953550663602 1101 48-49 2.0804414951524883 1101 50-51 2.3271518764737458 1101 52-53 2.1699534700695793 1101 54-55 2.6097829165187036 1101 56-57 2.2700601455491487 1101 58-59 2.322012531698018 1101 60-61 2.31865168910646 1101 62-63 2.3299173125754855 1101 64-65 2.580381396530292 1101 66-67 2.689031218576929 1101 68-69 2.5847655102326748 1101 70-71 2.2792783600544766 1101 72-73 2.46399028598125 1101 74-75 2.56701374223006 1101 76-77 2.3314582122482506 1101 78-79 2.3570725672127963 1101 80-81 2.4204881153083058 1101 82-83 2.3143249978196074 1101 84-85 2.6461325597869596 1101 86-87 2.4949583582481267 1101 88-89 2.3625925943331296 1101 90-91 2.3795096246053795 1101 92-93 2.2516066769185876 1101 94-95 2.1520261798068105 1101 96-97 2.0886523280658444 1101 98-99 2.2212267274118034 1101 100-101 2.294276793158417 1101 102-103 2.116241483232443 1101 104-105 2.1996994949027453 1101 106-107 2.3305219384075073 1101 108-109 2.263617321817712 1101 110-111 2.3052579538597904 1101 112-113 2.1382270958615273 1101 114-115 2.2108867651367525 1101 116-117 2.2120603825430187 1101 118-119 2.212281611950992 1101 120-121 2.039062415331813 1101 122-123 2.2076583470306375 1101 124-125 2.052394788289984 1102 1 0.6270004700192899 1102 2 -1.3935239375107251 1102 3 0.14147596769874582 1102 4 0.5922552635820963 1102 5 1.1751400578419258 1102 6 0.764676848906845 1102 7 1.4869961040961535 1102 8 1.222877368724788 1102 9 2.0093520375034473 1102 10-11 1.7520544228954016 1102 12-13 1.8501171902017752 1102 14-15 1.842833858874883 1102 16-17 1.7827021851986515 1102 18-19 1.8402988749201512 1102 20-21 2.036207433244819 1102 22-23 2.024968271026637 1102 24-25 2.231863094837138 1102 26-27 1.8787346915851657 1102 28-29 1.848708527932466 1102 30-31 2.288508297506027 1102 32-33 1.9521443883772562 1102 34-35 2.26099117473213 1102 36-37 1.656500113926338 1102 38-39 1.546493791893571 1102 40-41 1.7126997635662562 1102 42-43 1.6035638227853326 1102 44-45 1.6733568094906595 1102 46-47 1.488821877348581 1102 48-49 1.5350900446827858 1102 50-51 1.712137606963136 1102 52-53 1.625291806638824 1102 54-55 1.8968287753760578 1102 56-57 1.5592285963848838 1102 58-59 1.5282832727771627 1102 60-61 1.5771663824621172 1102 62-63 1.9019461903330388 1102 64-65 1.9773044282835492 1102 66-67 2.082175951075321 1102 68-69 1.998126383687115 1102 70-71 1.4824243130713057 1102 72-73 1.7412447904040533 1102 74-75 1.9579523209132823 1102 76-77 1.7718199018485734 1102 78-79 1.6021264647491797 1102 80-81 1.6610763226979266 1102 82-83 1.3885461074746992 1102 84-85 1.3468540669598106 1102 86-87 1.4460478408126072 1102 88-89 1.563147008163373 1102 90-91 1.3370024310079813 1102 92-93 1.330016827895264 1102 94-95 1.4177900975691031 1102 96-97 1.4802496652003327 1102 98-99 1.311761018459837 1102 100-101 1.1124056231264916 1102 102-103 0.8779976175811868 1102 104-105 1.2311887494268206 1102 106-107 1.4713650655116446 1102 108-109 1.4218328486321745 1102 110-111 1.296127663030628 1102 112-113 1.3418096935979413 1102 114-115 1.5533245675289926 1102 116-117 1.2016956513525017 1102 118-119 0.8247950318909858 1102 120-121 1.1520968607645052 1102 122-123 1.451770498116744 1102 124-125 1.4725260207139392 1103 1 0.7231688659738538 1103 2 0.5909619651572093 1103 3 0.45380110135274165 1103 4 0.4456234400167389 1103 5 0.9306822474992309 1103 6 0.8582707933170752 1103 7 1.0376309343901404 1103 8 0.9556388610532593 1103 9 1.7489823247884644 1103 10-11 1.353938954700947 1103 12-13 1.4302772697747521 1103 14-15 1.1359188318747613 1103 16-17 1.2396752940088334 1103 18-19 1.527653318792062 1103 20-21 1.6738105725950803 1103 22-23 1.519571001619795 1103 24-25 1.761457497677732 1103 26-27 1.2992466673302516 1103 28-29 1.4453048321219555 1103 30-31 1.943771090017222 1103 32-33 1.6847139204730652 1103 34-35 1.6525896005085947 1103 36-37 1.6920099441455072 1103 38-39 1.799032305130897 1103 40-41 1.9183660466116876 1103 42-43 1.8984645331129997 1103 44-45 1.910592995086727 1103 46-47 1.8247044066632903 1103 48-49 1.8227721226291145 1103 50-51 1.8933653325440893 1103 52-53 1.721205672002732 1103 54-55 2.1061167779662497 1103 56-57 1.954958760444299 1103 58-59 2.1335490875772827 1103 60-61 2.1342187590285455 1103 62-63 2.0693395229841123 1103 64-65 2.113242166737656 1103 66-67 2.432457712190015 1103 68-69 2.492565162586633 1103 70-71 2.197325992925329 1103 72-73 2.188312451972326 1103 74-75 2.1619408146703094 1103 76-77 2.1458183721514423 1103 78-79 2.2805992393121635 1103 80-81 2.168083155464803 1103 82-83 2.1192194973876717 1103 84-85 2.2612375589184275 1103 86-87 2.282933500739869 1103 88-89 2.1627656012848817 1103 90-91 2.124301695664137 1103 92-93 1.9013229175381454 1103 94-95 1.8477560324827635 1103 96-97 1.7733733893305867 1103 98-99 1.912536100914462 1103 100-101 1.9588623633993585 1103 102-103 1.809763340515694 1103 104-105 1.7983885358916325 1103 106-107 1.7679654692415667 1103 108-109 1.8108162763147853 1103 110-111 1.7322111895211414 1103 112-113 1.7391845214621249 1103 114-115 1.6357645002845587 1103 116-117 1.767581041368416 1103 118-119 1.8221448735050956 1103 120-121 1.858531643060953 1103 122-123 1.9575130864768013 1103 124-125 1.8249533675811414 1104 1 0.8420746715619387 1104 2 0.7616648523016956 1104 3 0.7323575632548867 1104 4 0.7518385343864296 1104 5 1.1840374560708646 1104 6 1.1930770062489628 1104 7 1.5922055566097804 1104 8 1.2522570766848347 1104 9 1.8456040686842812 1104 10-11 1.424374718097834 1104 12-13 1.5297162757602827 1104 14-15 1.6093891301496726 1104 16-17 1.4425857136560296 1104 18-19 1.3321853742108516 1104 20-21 1.6640877006790404 1104 22-23 1.646029284447735 1104 24-25 1.9082545166358926 1104 26-27 1.7750425470005027 1104 28-29 1.6483822183382841 1104 30-31 2.1763846574451975 1104 32-33 2.093305016272268 1104 34-35 2.0743082294023516 1104 36-37 2.0499392058619073 1104 38-39 2.1859518345287334 1104 40-41 2.2879879191184287 1104 42-43 2.150555655658721 1104 44-45 2.015050729326486 1104 46-47 2.016928510626439 1104 48-49 1.8093886765620546 1104 50-51 2.29420477489624 1104 52-53 2.0884825576607966 1104 54-55 2.3703057495227497 1104 56-57 2.2377983075062247 1104 58-59 2.3899609680396914 1104 60-61 2.4527178468015194 1104 62-63 2.5054082854397386 1104 64-65 2.5181099763343084 1104 66-67 2.4699182610741133 1104 68-69 2.55693504304676 1104 70-71 2.3585289147833777 1104 72-73 2.475882377047931 1104 74-75 2.5512694087480625 1104 76-77 2.381733598205056 1104 78-79 2.4117399271415856 1104 80-81 2.287382191793405 1104 82-83 2.268046463342202 1104 84-85 2.576444719246169 1104 86-87 2.4573610398666155 1104 88-89 2.2489922820190813 1104 90-91 2.351220815328066 1104 92-93 2.138402698421558 1104 94-95 2.1451585471108316 1104 96-97 2.1094362903149744 1104 98-99 2.1321953047331803 1104 100-101 2.1178927280966846 1104 102-103 1.9885913649494924 1104 104-105 2.0052004190856003 1104 106-107 2.126143473587021 1104 108-109 2.025762060551184 1104 110-111 2.0440093067938143 1104 112-113 2.00564711787764 1104 114-115 1.901459073245082 1104 116-117 1.92022752408732 1104 118-119 1.8833821105782746 1104 120-121 1.9373888610359806 1104 122-123 2.057544044150152 1104 124-125 1.7877028073269265 1105 1 0.5587277465598319 1105 2 0.6412149671087306 1105 3 0.5505896439165134 1105 4 0.7388977927232077 1105 5 0.9941847224237641 1105 6 0.9435430350158569 1105 7 1.281160608836796 1105 8 1.0539227973866616 1105 9 1.7392992369177733 1105 10-11 1.44754909681701 1105 12-13 1.502475472518487 1105 14-15 1.4117288005866158 1105 16-17 1.4101833857216803 1105 18-19 1.5577954928400004 1105 20-21 1.9174020114150068 1105 22-23 1.746075167090325 1105 24-25 1.8652357084827287 1105 26-27 1.6046817871908274 1105 28-29 1.6058501564429122 1105 30-31 1.9713616415179729 1105 32-33 1.9737811914446795 1105 34-35 2.1093210472737987 1105 36-37 1.8516533056352777 1105 38-39 1.8948042003112455 1105 40-41 2.034077105334241 1105 42-43 1.9880686901742877 1105 44-45 1.9305502819778972 1105 46-47 1.707893188165297 1105 48-49 1.6666130837175608 1105 50-51 1.7982773400602063 1105 52-53 1.8482686165684825 1105 54-55 2.0259599602523437 1105 56-57 1.7556127958616727 1105 58-59 1.6992592763790846 1105 60-61 1.5530000580522056 1105 62-63 1.4899134387003912 1105 64-65 1.820121065070019 1105 66-67 1.9894019995654304 1105 68-69 1.9941540728369063 1105 70-71 1.9036065307683323 1105 72-73 2.0731278402904536 1105 74-75 2.101491906565837 1105 76-77 1.8360121904945927 1105 78-79 1.9934939296058758 1105 80-81 1.9866584282574529 1105 82-83 1.865193504155659 1105 84-85 2.0672015905951397 1105 86-87 2.0539084499220017 1105 88-89 1.8828514914602863 1105 90-91 2.010974219797191 1105 92-93 1.748476391856638 1105 94-95 1.5912214777806994 1105 96-97 1.6963566955513905 1105 98-99 1.7394183919377504 1105 100-101 1.7569420133432487 1105 102-103 1.589683394798751 1105 104-105 1.2466946552041662 1105 106-107 1.406139186615917 1105 108-109 1.4182865757705052 1105 110-111 1.6022254562214435 1105 112-113 1.59114358299162 1105 114-115 1.4607876256778347 1105 116-117 1.5995925301217895 1105 118-119 1.63838165433436 1105 120-121 1.6879306148146291 1105 122-123 1.7557291689916852 1105 124-125 1.5700098979312287 1106 1 0.38756685109749967 1106 2 0.32375539619102156 1106 3 0.3505111007545274 1106 4 0.3990349709034362 1106 5 0.5842953124843966 1106 6 0.6576454568219248 1106 7 0.9803967604795751 1106 8 0.5624448476033272 1106 9 1.3624991064224972 1106 10-11 1.0139409990814308 1106 12-13 0.8785672210058273 1106 14-15 1.0723091238552769 1106 16-17 0.9615144756061582 1106 18-19 0.9751823190841975 1106 20-21 0.862976227235329 1106 22-23 0.7774465418270182 1106 24-25 0.5908284208955621 1106 26-27 0.6307205146510313 1106 28-29 0.6531694244393833 1106 30-31 1.1532342194410248 1106 32-33 0.9252634813087823 1106 34-35 0.9816313320059429 1106 36-37 1.0475034035799489 1106 38-39 1.1464577505822398 1106 40-41 1.326317292783557 1106 42-43 1.312276530257833 1106 44-45 1.251809883528022 1106 46-47 1.1912920278233265 1106 48-49 1.1888073254670886 1106 50-51 1.534362856636708 1106 52-53 1.3081109503956654 1106 54-55 1.7275720864731383 1106 56-57 1.3944867118298632 1106 58-59 1.3514930854910858 1106 60-61 1.5607425629933687 1106 62-63 1.6071521984915655 1106 64-65 1.6804269851217484 1106 66-67 1.8226044081052564 1106 68-69 1.6928203326332039 1106 70-71 1.604071112692921 1106 72-73 1.5719866509033302 1106 74-75 1.7991639013015117 1106 76-77 1.425882787560262 1106 78-79 1.3232702514489283 1106 80-81 1.4511102382790533 1106 82-83 1.341370853561152 1106 84-85 1.674541452011269 1106 86-87 1.7098250899146414 1106 88-89 1.4980232658241164 1106 90-91 1.5819118148828828 1106 92-93 1.467632049936757 1106 94-95 1.423619536399901 1106 96-97 1.3291736510345196 1106 98-99 1.3800722681540805 1106 100-101 1.1359231076351648 1106 102-103 0.9986554721482435 1106 104-105 1.2053260713561187 1106 106-107 1.2113435350137038 1106 108-109 1.17323870456255 1106 110-111 1.200175544121258 1106 112-113 1.0376509747465796 1106 114-115 1.1012683965136638 1106 116-117 1.40182363034398 1106 118-119 1.162948268570588 1106 120-121 1.1256226348352278 1106 122-123 1.3904720426347126 1106 124-125 1.3668324726768226 1107 1 0.5235184700115525 1107 2 0.5198271288402161 1107 3 0.30434973221549555 1107 4 0.4266378145636125 1107 5 0.39587102670842356 1107 6 0.8146954210567472 1107 7 0.9481915556605678 1107 8 0.9531400461516952 1107 9 1.6791549308896307 1107 10-11 1.1618346730100235 1107 12-13 1.3990629970069257 1107 14-15 1.1842882758544704 1107 16-17 1.277819952700483 1107 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12-13 1.5311699560570737 2204 14-15 1.2384971574242485 2204 16-17 0.9289967627863618 2204 18-19 0.7820614960604502 2204 20-21 1.3874680865551525 2204 22-23 1.6687833930294573 2204 24-25 1.681077718108142 2204 26-27 1.6625726564311307 2204 28-29 1.6787198829712864 2204 30-31 2.0001110934996973 2204 32-33 1.874033837652192 2204 34-35 1.711356821095901 2204 36-37 1.817134890917064 2204 38-39 1.903513148924585 2204 40-41 2.046215858782084 2204 42-43 1.8448919777855863 2204 44-45 1.7779712420218878 2204 46-47 1.7056468130544964 2204 48-49 1.8110207473095308 2204 50-51 2.142901921820478 2204 52-53 1.9044159980625182 2204 54-55 1.7588321613748334 2204 56-57 1.752992971465133 2204 58-59 1.5931489993085712 2204 60-61 1.9001597948411586 2204 62-63 1.7376170935947712 2204 64-65 2.085209352155392 2204 66-67 2.4411503698539576 2204 68-69 2.5450713388102812 2204 70-71 2.247831555405611 2204 72-73 2.232048476811695 2204 74-75 2.3991142678559996 2204 76-77 2.208730601677672 2204 78-79 2.224719888156134 2204 80-81 2.0900445654884727 2204 82-83 2.0554571643353547 2204 84-85 2.379573228269358 2204 86-87 2.0420212125976995 2204 88-89 2.0543635235222197 2204 90-91 1.9966260073147453 2204 92-93 1.7443242605327676 2204 94-95 1.8488331003755363 2204 96-97 1.8673269827462953 2204 98-99 1.897343366532592 2204 100-101 1.949933000778742 2204 102-103 1.9065802363879243 2204 104-105 1.8662654493141666 2204 106-107 1.9140402700766757 2204 108-109 1.7695327251542778 2204 110-111 1.7078023916133844 2204 112-113 1.7413990833855557 2204 114-115 1.6578595201708772 2204 116-117 1.6484916399923186 2204 118-119 1.6248601707137666 2204 120-121 1.5617722425296172 2204 122-123 0.9232974108505161 2204 124-125 1.292229898620871 2205 1 0.8345649816394598 2205 2 0.9355248032588221 2205 3 0.8251830655488277 2205 4 0.9547385897574117 2205 5 1.4363264530650106 2205 6 1.3842206148551952 2205 7 1.5477555091489386 2205 8 1.3271710539036334 2205 9 1.0090967913547502 2205 10-11 1.5966699443278536 2205 12-13 1.856669586154208 2205 14-15 1.8373890510482624 2205 16-17 1.6369527046384675 2205 18-19 1.8854453409882552 2205 20-21 2.005012297261864 2205 22-23 2.030281677265336 2205 24-25 2.2582955504912974 2205 26-27 1.9777275454184746 2205 28-29 1.9696328115988422 2205 30-31 2.29442620545975 2205 32-33 2.19732336779893 2205 34-35 2.2717033211599933 2205 36-37 2.083955283223087 2205 38-39 2.246469355490607 2205 40-41 2.2643588453959183 2205 42-43 2.1919034447743684 2205 44-45 2.1128769237577494 2205 46-47 2.0603106890792127 2205 48-49 2.1487263011467697 2205 50-51 2.2867939617337605 2205 52-53 2.2798263917060453 2205 54-55 2.3904098667510354 2205 56-57 2.2319077245288454 2205 58-59 2.3294681662678194 2205 60-61 2.4380445356165765 2205 62-63 2.0001505137264033 2205 64-65 2.4953238269908518 2205 66-67 2.6230239801059128 2205 68-69 2.7127648168167333 2205 70-71 2.390122511524403 2205 72-73 2.413567770972943 2205 74-75 2.5531357075931815 2205 76-77 2.304622084995124 2205 78-79 2.4073151921313 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2.144468782391421 2206 82-83 2.0155639617538164 2206 84-85 2.2547759975366866 2206 86-87 2.1555509932174637 2206 88-89 1.6598361913947102 2206 90-91 1.8398442436358344 2206 92-93 1.7854402434902923 2206 94-95 1.6770520583629462 2206 96-97 1.6308077584579763 2206 98-99 1.704124753714936 2206 100-101 1.8571802837812115 2206 102-103 1.6024780677504609 2206 104-105 1.580890306123326 2206 106-107 1.4767229535395714 2206 108-109 1.4345690754666833 2206 110-111 1.455594727833212 2206 112-113 1.4121883299768392 2206 114-115 1.4490528573657429 2206 116-117 1.3747178505106916 2206 118-119 1.3791344845027922 2206 120-121 1.471378607034513 2206 122-123 0.47256721731461226 2206 124-125 1.157011817726758 2207 1 0.1510395132106268 2207 2 0.28101141573553434 2207 3 0.13108308876500274 2207 4 0.281377276684438 2207 5 0.1930267619534085 2207 6 0.4033599812800759 2207 7 -0.341918282335822 2207 8 0.2506296908875676 2207 9 0.47338670194415755 2207 10-11 -0.3147388422871664 2207 12-13 0.16949597516106607 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1.517166157556705 2208 80-81 1.458122289801505 2208 82-83 1.5109266960459244 2208 84-85 1.6102845054103128 2208 86-87 1.5230613493812015 2208 88-89 1.297684022376231 2208 90-91 1.3728179746654767 2208 92-93 1.25545482673472 2208 94-95 1.191887941679333 2208 96-97 1.2838443254651146 2208 98-99 1.2670499376977986 2208 100-101 1.3115749908434147 2208 102-103 1.0922495478688319 2208 104-105 1.13832439088862 2208 106-107 1.3035349032649037 2208 108-109 1.2920223789173662 2208 110-111 1.30464487447502 2208 112-113 1.2164908727001524 2208 114-115 1.1853154852634482 2208 116-117 1.1050212732320013 2208 118-119 1.090370734534062 2208 120-121 1.2130357698208982 2208 122-123 1.3114428506409297 2208 124-125 1.2536428086663136 2209 1 0.1507406668009068 2209 2 0.2550957762185959 2209 3 0.22304594170093495 2209 4 0.32383689287649986 2209 5 0.42755211381090774 2209 6 0.3839364025619645 2209 7 0.48376768640585155 2209 8 0.49067705097376546 2209 9 0.932403343878704 2209 10-11 0.3251689982251911 2209 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-5.656887494982843 2216 122-123 -5.601106509724261 2216 124-125 -5.5059216417303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 133260.0 3 44059.0 4 21085.0 5 17119.0 6 17043.0 7 19768.0 8 21895.0 9 23326.0 10 24463.0 11 26476.0 12 28375.0 13 29698.0 14 30248.0 15 25207.0 16 18863.0 17 16447.0 18 14803.0 19 13700.0 20 13445.0 21 14350.0 22 17544.0 23 23804.0 24 32694.0 25 43029.0 26 52834.0 27 60179.0 28 67947.0 29 76794.0 30 85749.0 31 97996.0 32 114095.0 33 135569.0 34 165316.0 35 208046.0 36 291108.0 37 382921.0 38 138708.0 39 631.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.90287052021305 20.063710779769668 14.343527581818469 24.689891118198815 2 15.664529272310215 20.791529106728202 38.9014143529 24.642527268061585 3 19.176416486894343 24.65739933469199 29.554413139166147 26.61177103924752 4 12.888941296700274 17.53906019692235 36.863313876933354 32.708684629444015 5 13.15431174992957 38.48055045252402 34.32735853572597 14.037779261820438 6 31.578742921088555 37.612351295284334 17.10160633856119 13.707299445065921 7 27.471131680515164 32.706386062035044 20.99173015230666 18.83075210514313 8 25.425469886533513 36.50365652591193 19.419060077831148 18.6518135097234 9 25.365907633777685 16.36007932216744 20.077462318439107 38.19655072561577 10-11 24.754759772382382 27.05569490371571 27.29987081003189 20.88967451387002 12-13 25.481278753813406 24.184142118634924 27.841028810217473 22.493550317334197 14-15 22.289266184475924 25.631465251288315 25.930082930608517 26.14918563362724 16-17 22.094645512428247 28.995579144265314 26.446479942915186 22.46329540039125 18-19 21.88486527749957 27.54676329141217 28.70467502447957 21.86369640660869 20-21 22.610539863410654 26.707180456069512 28.825041249511973 21.85723843100786 22-23 22.191272124861737 26.54377180409072 28.71345908080173 22.551496990245816 24-25 22.073642965989613 26.79925987008291 28.691982625664046 22.435114538263427 26-27 22.040301575131274 27.042840337361085 28.641377665013657 22.27548042249398 28-29 21.8981152494382 27.096864948771966 28.831939641523448 22.173080160266384 30-31 21.99910145689544 26.666934790882728 28.780806491483457 22.553157260738374 32-33 21.92461132958682 26.8029510406234 28.68322417177324 22.589213458016534 34-35 22.349165342553178 26.92013785821447 28.467344604925017 22.263352194307334 36-37 22.34766115166121 26.699997881158083 28.48990921154753 22.462431755633176 38-39 22.065073555738447 26.990056217635132 28.677997824276485 22.26687240234994 40-41 22.314527251717152 26.82390609580723 28.502092362142466 22.35947429033315 42-43 22.22677596968499 26.850069990161156 28.29872408966196 22.62442995049189 44-45 22.31858155332663 26.992687053976503 28.15253696936999 22.536194423326876 46-47 22.41501081578866 27.15074890458995 27.765834349908907 22.668405929712478 48-49 22.481454480391932 27.24555186114383 27.579539934567844 22.693453723896393 50-51 22.409015812056225 27.289250761399003 27.438213731834466 22.863519694710302 52-53 22.68510952614041 27.309622860461214 27.299205345695714 22.706062267702663 54-55 22.408384307064697 27.12499904230874 27.382171069394406 23.084445581232156 56-57 22.418356816906602 27.438779874361902 27.335055854413614 22.807807454317878 58-59 22.543586777650738 27.21200395198294 27.406640681097105 22.83776858926922 60-61 22.60689619452922 27.229209517090602 27.58014811303801 22.58374617534217 62-63 22.400881904247704 27.277840645128887 27.53396406463845 22.78731338598496 64-65 22.23755745641792 27.12121557129778 27.424986690997144 23.21624028128715 66-67 22.190609290995777 27.385079525223716 27.21876650028167 23.205544683498836 68-69 22.213467768262976 27.202414398181862 27.496758933728433 23.087358899826725 70-71 22.264617140081306 27.15540978923679 27.511262893985478 23.068710176696428 72-73 22.42421232172109 27.113491478892378 27.27033215907324 23.191964040313295 74-75 22.31041100717417 27.289168658402545 27.171449906550933 23.228970427872348 76-77 22.310928540850924 27.177878218451536 27.310698998291965 23.200494242405576 78-79 22.338460500163134 27.296027523117573 27.276761654722286 23.08875032199701 80-81 22.23324574245142 27.37653864429404 27.2487425404861 23.14147307276844 82-83 22.39703773633397 27.338394560289476 27.227725588820334 23.036842114556215 84-85 22.313832646549432 27.19954610267465 27.085816728753187 23.40080452202273 86-87 22.315986652203744 27.11984746048347 27.3416567739242 23.222509113388583 88-89 22.30594063737649 27.130259815703518 27.20302649754871 23.360773049371282 90-91 22.295258407166802 27.31023829109845 27.231469076265668 23.16303422546908 92-93 22.343747639570193 27.200881920338766 27.262407596223625 23.192962843867416 94-95 22.28926843375158 27.157678798944158 27.27366900648266 23.279383760821602 96-97 22.258609232739598 27.11978696726366 27.284254423680178 23.337349376316567 98-99 21.969819437697804 26.95480331508275 27.239725119026414 23.835652128193036 100-101 22.013520571020273 27.09434853629166 27.31284438551794 23.579286507170124 102-103 22.25704800242019 26.983817129601473 27.305037866496324 23.454097001482012 104-105 22.244223515967178 26.859289704591266 27.33518076189262 23.561306017548937 106-107 21.962671553023874 26.97369234194953 27.39031445788214 23.673321647144455 108-109 22.12492456625104 26.989959954390542 27.126603923575114 23.758511555783308 110-111 21.849192209727068 27.086973828846826 27.249985629300298 23.813848332125804 112-113 21.9859000605236 27.072727397573367 27.24947201249317 23.69190052940986 114-115 21.8707096358024 27.092873743079014 27.180216190213486 23.8562004309051 116-117 21.8222282560502 27.030158589402625 27.209080771594063 23.93853238295311 118-119 21.8499853741596 27.086369756547935 27.017056417683392 24.046588451609068 120-121 21.72893790428761 27.107308238849555 27.149685418110714 24.014068438752126 122-123 21.780739250296833 27.086140449865848 27.04272415013525 24.09039614970207 124-125 21.713405167483415 27.029255225745086 26.99596004028384 24.26137956648766 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 172.0 1 143.0 2 189.0 3 298.5 4 255.0 5 213.0 6 395.5 7 870.5 8 1399.0 9 1660.0 10 1437.5 11 1058.5 12 1032.5 13 1153.5 14 1352.0 15 1572.5 16 1794.5 17 2078.0 18 2399.5 19 2752.5 20 3258.0 21 3957.0 22 4741.0 23 5876.5 24 7486.0 25 9577.0 26 12576.0 27 16336.5 28 20239.0 29 24506.5 30 29372.5 31 34420.5 32 39399.5 33 44493.0 34 50306.5 35 55823.0 36 61654.0 37 67510.5 38 71873.0 39 75247.0 40 78035.5 41 81869.5 42 85678.5 43 89172.5 44 92747.5 45 95414.5 46 96285.5 47 97010.0 48 100647.0 49 101690.0 50 98228.0 51 94163.0 52 89968.0 53 87415.5 54 84547.0 55 78418.5 56 71100.5 57 63924.5 58 57800.5 59 51347.0 60 43112.0 61 34738.5 62 27328.5 63 21220.0 64 16126.5 65 12213.0 66 9369.5 67 7301.0 68 5534.5 69 3888.5 70 2815.0 71 2238.0 72 1775.0 73 1400.5 74 1089.5 75 803.0 76 540.5 77 309.5 78 182.5 79 121.0 80 76.5 81 50.5 82 32.5 83 20.0 84 14.0 85 8.0 86 6.0 87 6.0 88 6.0 89 3.5 90 1.5 91 2.0 92 1.5 93 0.0 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10649008826042908 2 3.138985652481329E-4 3 0.0 4 0.17437065299533783 5 0.0 6 1.1771196196804983E-4 7 0.03550977519369503 8 0.0 9 0.0 10-11 8.436023941043572E-4 12-13 7.455090924643156E-4 14-15 6.866531114802907E-4 16-17 0.004139537329209752 18-19 0.0015891114865686725 20-21 0.0030997483318253123 22-23 0.0018441540708327808 24-25 0.004806571780362035 26-27 0.004237630630849794 28-29 0.0034136468970734453 30-31 9.416956957443986E-4 32-33 5.885598098402491E-5 34-35 0.0013144502419765564 36-37 0.0012555942609925317 38-39 6.47415790824274E-4 40-41 0.001530255505584648 42-43 8.436023941043572E-4 44-45 0.0012359756006645232 46-47 1.9618660328008306E-4 48-49 0.0 50-51 0.038491811563552296 52-53 5.885598098402491E-5 54-55 0.13381888209734466 56-57 1.5694928262406646E-4 58-59 0.0 60-61 0.0 62-63 7.06271771808299E-4 64-65 0.056737165668600015 66-67 0.050576906325605415 68-69 0.0019618660328008302 70-71 0.002766231106249171 72-73 0.06835141258278093 74-75 0.005767886136434442 76-77 0.0018441540708327808 78-79 0.0017852980898487559 80-81 1.1771196196804983E-4 82-83 9.809330164004153E-5 84-85 0.0 86-87 3.335172255761412E-4 88-89 0.001471399524600623 90-91 3.727545462321578E-4 92-93 0.0025308071823130713 94-95 0.004041444027569711 96-97 0.0025700445029690883 98-99 0.0 100-101 0.001294831581648548 102-103 0.0013733062229605813 104-105 2.5504258426410794E-4 106-107 5.885598098402491E-5 108-109 0.0 110-111 2.1580526360809134E-4 112-113 5.885598098402491E-5 114-115 1.9618660328008306E-4 116-117 0.0 118-119 8.828397147603738E-4 120-121 0.0020599593344408722 122-123 4.708478478721993E-4 124-125 0.006964624416442949 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2548594.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.35141586774565 #Duplication Level Percentage of deduplicated Percentage of total 1 80.38202165759517 50.9231488232045 2 11.103931931188555 14.068996190797323 3 3.762581931789467 7.150946780917809 4 1.6891422068609823 4.280382016264469 5 0.8747813409020729 2.77093182604157 6 0.540625032072323 2.054961674119624 7 0.3368301398781781 1.493706638774938 8 0.23274556792634904 1.1795809012061427 9 0.17095528882844244 0.9747233637625107 >10 0.8270742182388123 9.370885553547199 >50 0.05450134590263037 2.3747679311870447 >100 0.024055971347420317 2.649417069040574 >500 5.022449796667057E-4 0.20450974876584394 >1k 2.511224898283355E-4 0.5030414823704692 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4564 0.1790791314740598 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4346 0.1705253955710482 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.923732065601661E-5 0.0 0.0 0.0 3.923732065601661E-5 6 3.923732065601661E-5 0.0 0.0 0.0 3.923732065601661E-5 7 3.923732065601661E-5 0.0 0.0 3.923732065601661E-5 3.923732065601661E-5 8 3.923732065601661E-5 0.0 0.0 3.923732065601661E-5 3.923732065601661E-5 9 3.923732065601661E-5 0.0 0.0 3.923732065601661E-5 3.923732065601661E-5 10-11 3.923732065601661E-5 0.0 0.0 9.809330164004153E-5 3.923732065601661E-5 12-13 3.923732065601661E-5 0.0 0.0 1.5694928262406643E-4 3.923732065601661E-5 14-15 3.923732065601661E-5 0.0 0.0 1.5694928262406643E-4 3.923732065601661E-5 16-17 3.923732065601661E-5 0.0 0.0 1.5694928262406643E-4 5.885598098402491E-5 18-19 3.923732065601661E-5 0.0 0.0 1.5694928262406643E-4 7.847464131203322E-5 20-21 3.923732065601661E-5 0.0 0.0 1.7656794295207475E-4 7.847464131203322E-5 22-23 3.923732065601661E-5 0.0 0.0 3.1389856524813287E-4 7.847464131203322E-5 24-25 3.923732065601661E-5 0.0 0.0 3.923732065601661E-4 7.847464131203322E-5 26-27 3.923732065601661E-5 0.0 0.0 5.10085168528216E-4 7.847464131203322E-5 28-29 3.923732065601661E-5 0.0 0.0 0.0013340689023045647 7.847464131203322E-5 30-31 5.885598098402491E-5 0.0 0.0 0.0036686894813375533 7.847464131203322E-5 32-33 7.847464131203322E-5 0.0 0.0 0.008651829204651663 7.847464131203322E-5 34-35 7.847464131203322E-5 3.923732065601661E-5 0.0 0.014851325868302288 7.847464131203322E-5 36-37 7.847464131203322E-5 3.923732065601661E-5 0.0 0.026779471347731336 7.847464131203322E-5 38-39 7.847464131203322E-5 3.923732065601661E-5 0.0 0.04726135273017201 7.847464131203322E-5 40-41 7.847464131203322E-5 3.923732065601661E-5 0.0 0.07178467814018238 7.847464131203322E-5 42-43 9.809330164004153E-5 3.923732065601661E-5 0.0 0.09546440115608842 7.847464131203322E-5 44-45 1.1771196196804984E-4 3.923732065601661E-5 0.0 0.12404878925399651 7.847464131203322E-5 46-47 1.1771196196804984E-4 3.923732065601661E-5 0.0 0.15859725009161912 7.847464131203322E-5 48-49 1.1771196196804984E-4 3.923732065601661E-5 0.0 0.19644164586434718 7.847464131203322E-5 50-51 1.1771196196804984E-4 3.923732065601661E-5 0.0 0.23936727466202934 7.847464131203322E-5 52-53 1.1771196196804984E-4 3.923732065601661E-5 0.0 0.2839016336066082 7.847464131203322E-5 54-55 1.1771196196804984E-4 3.923732065601661E-5 0.0 0.3304959518856279 1.1771196196804984E-4 56-57 1.5694928262406643E-4 7.847464131203322E-5 0.0 0.3866837950650437 1.1771196196804984E-4 58-59 1.5694928262406643E-4 7.847464131203322E-5 0.0 0.4494046521336863 1.1771196196804984E-4 60-61 1.5694928262406643E-4 7.847464131203322E-5 0.0 0.5191489895997558 1.1771196196804984E-4 62-63 1.5694928262406643E-4 7.847464131203322E-5 0.0 0.5900115907045218 1.1771196196804984E-4 64-65 1.5694928262406643E-4 7.847464131203322E-5 0.0 0.6656415262689938 1.5694928262406643E-4 66-67 1.5694928262406643E-4 7.847464131203322E-5 0.0 0.7552988039679918 1.5694928262406643E-4 68-69 1.5694928262406643E-4 7.847464131203322E-5 0.0 0.8571588883910108 1.5694928262406643E-4 70-71 1.7656794295207475E-4 7.847464131203322E-5 0.0 0.9768327163918615 1.5694928262406643E-4 72-73 1.9618660328008306E-4 7.847464131203322E-5 0.0 1.1004891324392978 1.5694928262406643E-4 74-75 1.9618660328008306E-4 7.847464131203322E-5 0.0 1.225715041312975 1.5694928262406643E-4 76-77 1.9618660328008306E-4 7.847464131203322E-5 0.0 1.3614369334621363 1.5694928262406643E-4 78-79 1.9618660328008306E-4 7.847464131203322E-5 0.0 1.520936641928844 1.5694928262406643E-4 80-81 2.3542392393609968E-4 7.847464131203322E-5 0.0 1.7063526006888505 1.5694928262406643E-4 82-83 2.3542392393609968E-4 7.847464131203322E-5 0.0 1.9018917881781092 1.5694928262406643E-4 84-85 2.3542392393609968E-4 7.847464131203322E-5 0.0 2.0957437708791593 1.5694928262406643E-4 86-87 2.3542392393609968E-4 7.847464131203322E-5 0.0 2.3061539028970484 1.5694928262406643E-4 88-89 2.3542392393609968E-4 7.847464131203322E-5 0.0 2.536712399071802 1.5694928262406643E-4 90-91 2.3542392393609968E-4 7.847464131203322E-5 0.0 2.8041147393425554 1.5694928262406643E-4 92-93 2.3542392393609968E-4 7.847464131203322E-5 0.0 3.0860152695957064 1.5694928262406643E-4 94-95 2.3542392393609968E-4 7.847464131203322E-5 0.0 3.3766461036948217 1.5694928262406643E-4 96-97 2.3542392393609968E-4 7.847464131203322E-5 0.0 3.668414820092961 1.5694928262406643E-4 98-99 2.3542392393609968E-4 7.847464131203322E-5 0.0 3.993829538953635 1.5694928262406643E-4 100-101 2.3542392393609968E-4 7.847464131203322E-5 0.0 4.369212985669746 1.5694928262406643E-4 102-103 2.3542392393609968E-4 7.847464131203322E-5 0.0 4.775947051590014 1.5694928262406643E-4 104-105 2.746612445921163E-4 7.847464131203322E-5 0.0 5.184623364882755 1.5694928262406643E-4 106-107 2.746612445921163E-4 7.847464131203322E-5 0.0 5.606012570068046 1.5694928262406643E-4 108-109 2.746612445921163E-4 7.847464131203322E-5 0.0 6.0411544561432695 1.5694928262406643E-4 110-111 2.746612445921163E-4 7.847464131203322E-5 0.0 6.520634514559792 1.5694928262406643E-4 112-113 2.746612445921163E-4 7.847464131203322E-5 0.0 7.0335251515149135 1.7656794295207475E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5245 0.0 51.19609 1 GTATCAA 8300 0.0 43.783348 1 ATCAACG 10335 0.0 33.963364 3 TATCAAC 10830 0.0 33.40282 2 TCAACGC 10410 0.0 33.1462 4 CAACGCA 10585 0.0 32.322792 5 AACGCAG 10840 0.0 31.61732 6 GTACATG 8210 0.0 29.000101 1 TACATGG 8380 0.0 27.664864 2 ACGCAGA 12670 0.0 26.722437 7 CGCAGAG 12920 0.0 26.513172 8 ACATGGG 8190 0.0 26.268131 3 GAGTACT 8115 0.0 24.807354 12-13 GCAGAGT 13885 0.0 23.856737 9 AGAGTAC 11800 0.0 23.637476 10-11 GTACTTT 8885 0.0 22.925665 14-15 AGTACTT 8690 0.0 22.549967 12-13 CAGAGTA 13390 0.0 22.074257 10-11 CATGGGG 4505 0.0 21.297909 4 ACTTTTT 11605 0.0 18.116388 16-17 >>END_MODULE