FastQCFastQC Report
Thu 26 May 2016
SRR1364471_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364471_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939398
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22560.11632475644504119No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53000.049.4126361
GGTATCA31850.044.102561
ATCAACG62700.039.7583583
TCAACGC63700.039.2833374
TATCAAC68500.038.5623172
CAACGCA63900.038.414475
AACGCAG65650.036.5585866
GTACATG72850.034.886671
TACATGG73550.032.1937182
CGCAGAG75050.030.7131378
ACGCAGA76650.030.6135947
ACATGGG76450.028.7164553
GTATACG1306.3660744E-527.4706631
GCAGAGT79850.027.3004689
CGCATAG3002.2191671E-1023.8245078
AGAGTAC73200.023.8241710-11
GAGTACT41250.023.21546712-13
GAGTACA55000.022.6175121
CATGGGA43100.022.532474
CATGGGG40850.022.0233524
Produced by FastQC (version 0.11.5)