FastQCFastQC Report
Thu 26 May 2016
SRR1364471_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364471_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939398
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42420.21872766703894714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39010.20114489135288371No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24670.12720442116574318No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37650.049.934221
GTATCAA63650.049.5094641
ATCAACG78350.037.7428673
TATCAAC82300.037.4526822
TCAACGC79800.036.534084
CAACGCA79900.036.415885
AACGCAG82550.034.8143926
GTACATG70300.030.6481721
TACATGG70500.029.85232
ACGCAGA98500.028.9957087
CGCAGAG99100.028.9245688
ACATGGG71350.026.321393
GCAGAGT107950.025.1209329
GAGTACT64400.024.19656812-13
AGAGTAC98600.023.76608310-11
AGTACTT66400.023.06468412-13
GTACTTT69400.022.79609714-15
TATAACG2454.2652573E-721.9012372
ACCGTCG1653.2275822E-421.6252358
CAGAGTA106650.021.3587710-11
Produced by FastQC (version 0.11.5)