##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364471_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1939398 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.832635178545093 31.0 28.0 31.0 25.0 34.0 2 29.05716103656908 31.0 28.0 33.0 25.0 34.0 3 29.105189857883737 31.0 28.0 34.0 25.0 34.0 4 32.533026743350256 35.0 32.0 37.0 26.0 37.0 5 31.602223473469603 35.0 32.0 37.0 19.0 37.0 6 31.637009525636305 35.0 32.0 37.0 19.0 37.0 7 31.08077764337181 35.0 31.0 37.0 17.0 37.0 8 31.203610604940295 35.0 31.0 37.0 17.0 37.0 9 31.76882878088974 37.0 30.0 39.0 10.0 39.0 10-11 31.961515119640218 37.0 30.5 39.0 12.0 39.0 12-13 31.694923630941148 37.0 30.0 39.0 10.5 39.0 14-15 32.63911610716315 37.5 31.0 40.0 10.5 41.0 16-17 32.71013015378999 38.0 31.0 40.0 10.5 41.0 18-19 32.56476081753204 38.0 31.0 40.0 10.0 41.0 20-21 32.44203665261076 38.0 30.5 40.0 10.0 41.0 22-23 32.41356854034087 37.5 30.5 40.0 10.0 41.0 24-25 32.21133233096043 37.0 30.0 40.0 9.5 41.0 26-27 32.311082356483816 37.0 30.0 40.0 9.5 41.0 28-29 32.193954515782735 37.0 30.0 40.0 8.0 41.0 30-31 31.679988584086402 37.0 30.0 40.0 8.0 41.0 32-33 31.61606049918583 37.0 30.0 40.0 3.5 41.0 34-35 31.411138146992002 37.0 30.0 40.0 2.0 41.0 36-37 31.39467401740128 37.0 30.0 40.0 2.0 41.0 38-39 31.161342076252527 36.0 30.0 40.0 2.0 41.0 40-41 30.87301575024827 36.0 28.5 40.0 2.0 41.0 42-43 30.759422769333575 36.0 28.5 40.0 2.0 41.0 44-45 30.69776317187086 36.0 28.0 40.0 2.0 41.0 46-47 30.55764907460975 36.0 28.0 40.0 2.0 41.0 48-49 30.297111010736323 36.0 28.0 40.0 2.0 41.0 50-51 29.95348891769508 36.0 27.0 40.0 2.0 41.0 52-53 29.861453657268903 35.0 27.0 40.0 2.0 41.0 54-55 29.39252927970432 35.0 26.0 40.0 2.0 41.0 56-57 29.409187026077163 35.0 26.0 39.0 2.0 41.0 58-59 29.167639133380565 35.0 26.0 39.0 2.0 41.0 60-61 28.882530816263603 35.0 26.0 39.0 2.0 41.0 62-63 28.614264581071033 34.0 25.5 39.0 2.0 40.5 64-65 28.138108320210705 34.0 23.0 38.0 2.0 40.0 66-67 27.742197063212398 34.0 22.5 38.0 2.0 40.0 68-69 27.560567248187326 34.0 22.0 37.5 2.0 40.0 70-71 27.640810447365624 34.0 23.5 37.0 2.0 40.0 72-73 27.412273550864754 34.0 23.5 37.0 2.0 39.0 74-75 27.03958444837006 34.0 22.0 36.0 2.0 39.0 76-77 26.950730845344793 33.0 23.0 36.0 2.0 39.0 78-79 26.554953650565793 33.0 22.0 35.5 2.0 37.5 80-81 26.32983688752902 33.0 21.5 35.0 2.0 37.0 82-83 26.19091207684034 33.0 21.5 35.0 2.0 37.0 84-85 25.7601760958813 33.0 19.5 35.0 2.0 36.0 86-87 25.632135590528605 33.0 20.0 35.0 2.0 36.0 88-89 25.586700099721668 33.0 20.0 35.0 2.0 36.0 90-91 25.427792026185443 33.0 19.5 35.0 2.0 35.0 92-93 25.448008608856973 33.0 20.0 35.0 2.0 35.0 94-95 25.4668951396258 33.0 20.0 35.0 2.0 35.0 96-97 25.350222594846443 33.0 19.0 35.0 2.0 35.0 98-99 25.21190209539249 33.0 18.5 35.0 2.0 35.0 100-101 25.118177908815003 33.0 18.0 35.0 2.0 35.0 102-103 24.01371301816337 30.5 16.0 34.0 2.0 35.0 104-105 24.95355285506121 32.0 18.0 35.0 2.0 35.0 106-107 25.047920798103327 33.0 18.0 35.0 2.0 35.0 108-109 25.127052569921183 33.0 18.0 35.0 2.0 35.0 110-111 25.056981599444775 33.0 17.5 35.0 2.0 35.0 112-113 25.009386675659147 33.0 17.0 35.0 2.0 35.0 114-115 24.959730803063632 33.0 15.0 35.0 2.0 35.0 116-117 24.88506485002047 33.0 8.5 35.0 2.0 35.0 118-119 24.773396951012636 33.0 4.5 35.0 2.0 35.0 120-121 24.614255042028507 33.0 2.0 35.0 2.0 35.0 122-123 24.328962904983918 33.0 2.0 35.0 2.0 35.0 124-125 23.57540303743739 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8188500045841991 1101 2 0.8463685010851343 1101 3 0.8173122438561116 1101 4 0.8841048926835455 1101 5 1.065039626866504 1101 6 1.0190237181429858 1101 7 1.540134105657934 1101 8 1.366343315786363 1101 9 2.0164840961066623 1101 10-11 1.5155060712173878 1101 12-13 1.8535245643738598 1101 14-15 1.783393305496837 1101 16-17 1.698569047760408 1101 18-19 1.7044766365221449 1101 20-21 1.9759464810806122 1101 22-23 1.9143580578753685 1101 24-25 2.048391413379875 1101 26-27 1.7974041939343977 1101 28-29 1.7714650141440629 1101 30-31 2.2749926750229577 1101 32-33 2.1543652778834392 1101 34-35 2.1034417053350865 1101 36-37 2.0187433623397837 1101 38-39 2.044593004733496 1101 40-41 2.3165910789681377 1101 42-43 2.2562901213297444 1101 44-45 2.1131520809989226 1101 46-47 1.8918354056508093 1101 48-49 1.9782788982502453 1101 50-51 2.2541379515915025 1101 52-53 2.102129913995313 1101 54-55 2.369100633324038 1101 56-57 2.1954969385281693 1101 58-59 2.3295330493544917 1101 60-61 2.345020331136798 1101 62-63 2.4724173000162146 1101 64-65 2.479920054039617 1101 66-67 2.6825184456213407 1101 68-69 2.5207345442235933 1101 70-71 2.2126996092103504 1101 72-73 2.4049906566618517 1101 74-75 2.3963427051018265 1101 76-77 2.2268691804506417 1101 78-79 2.3789187682143087 1101 80-81 2.4255676962598756 1101 82-83 2.248579790287433 1101 84-85 2.452739191291336 1101 86-87 2.339504947222327 1101 88-89 2.225545317298284 1101 90-91 2.277856758360656 1101 92-93 2.104599200380111 1101 94-95 2.0129933190713416 1101 96-97 1.9192215959877679 1101 98-99 2.0714412371488358 1101 100-101 2.1419568091883896 1101 102-103 1.8948060252136791 1101 104-105 1.9518015322611681 1101 106-107 2.0476356075211584 1101 108-109 1.9821274502117454 1101 110-111 1.9860791429183635 1101 112-113 1.899884551194365 1101 114-115 1.8643040652123162 1101 116-117 1.9651632206014185 1101 118-119 1.7725789148004694 1101 120-121 1.8335896108033403 1101 122-123 1.8881190767768885 1101 124-125 1.734660324879144 1102 1 0.46328073477789644 1102 2 -1.6301014507729263 1102 3 -0.01810527312142085 1102 4 0.47208809463872825 1102 5 0.9041821201172873 1102 6 0.3665701512630619 1102 7 1.1371777284493803 1102 8 0.9706229815957563 1102 9 1.646467327566608 1102 10-11 1.3631803583085613 1102 12-13 1.6322794142192087 1102 14-15 1.6599392321184894 1102 16-17 1.5655566606775864 1102 18-19 1.675907420807917 1102 20-21 1.8975904372758734 1102 22-23 1.8948799971250665 1102 24-25 1.9767218863903935 1102 26-27 1.7817519173960932 1102 28-29 1.707308137321526 1102 30-31 2.132677682177871 1102 32-33 1.7875731340182277 1102 34-35 2.1003547695521974 1102 36-37 1.4493875004810874 1102 38-39 1.386184393276796 1102 40-41 1.6622713358562677 1102 42-43 1.5209392359410039 1102 44-45 1.3407260027227892 1102 46-47 1.1333183641917017 1102 48-49 1.182032641667 1102 50-51 1.3572374268984717 1102 52-53 1.227328456460043 1102 54-55 1.5720184084682458 1102 56-57 1.1476180850832414 1102 58-59 1.0286213521913758 1102 60-61 1.1456861552785789 1102 62-63 1.4435530366763736 1102 64-65 1.542537765748584 1102 66-67 1.6408171518375028 1102 68-69 1.5742106969992555 1102 70-71 1.0035796871029241 1102 72-73 1.297165501884784 1102 74-75 1.4444674315680928 1102 76-77 1.4153603560679038 1102 78-79 1.2903331773568532 1102 80-81 1.3526589692428566 1102 82-83 0.953619449605771 1102 84-85 1.049166902164334 1102 86-87 1.0990620315616901 1102 88-89 1.2133328043284806 1102 90-91 0.9452483406519008 1102 92-93 0.9207845296286763 1102 94-95 0.8606885053693318 1102 96-97 0.8998025447625828 1102 98-99 0.7898477832721582 1102 100-101 0.6153250728822854 1102 102-103 0.4385800285673902 1102 104-105 0.6382089340226003 1102 106-107 0.9326813644904277 1102 108-109 0.7427909631471259 1102 110-111 0.6921453221007887 1102 112-113 0.7102500660278857 1102 114-115 0.9482819956499213 1102 116-117 0.5690433427511365 1102 118-119 0.1870741129003619 1102 120-121 0.522811029293976 1102 122-123 0.772028900387582 1102 124-125 0.7119355108820855 1103 1 0.7016095112645679 1103 2 0.688746806233226 1103 3 0.5472057316632686 1103 4 0.6526417515081775 1103 5 0.8791992082922739 1103 6 0.9960301649336039 1103 7 1.2400827181964758 1103 8 1.0640098578773696 1103 9 1.924474846363598 1103 10-11 1.592384049354358 1103 12-13 1.6542206308972673 1103 14-15 1.430077646335853 1103 16-17 1.4558847536372639 1103 18-19 1.508973039399848 1103 20-21 1.7768539369341099 1103 22-23 1.5400611317798862 1103 24-25 1.9673148460623011 1103 26-27 1.4631941593646545 1103 28-29 1.4743450477794866 1103 30-31 2.1137867047342596 1103 32-33 1.9039179733893796 1103 34-35 2.081446703933608 1103 36-37 2.0554281703375423 1103 38-39 2.0674557534955262 1103 40-41 2.3046072893764418 1103 42-43 2.145853795066092 1103 44-45 2.060317595340699 1103 46-47 2.110121166652398 1103 48-49 2.1488046854127276 1103 50-51 2.144848500035863 1103 52-53 1.994860923992512 1103 54-55 2.3694124617833516 1103 56-57 2.1532400946526202 1103 58-59 2.1391903884183066 1103 60-61 2.0436270301931394 1103 62-63 2.177934444741993 1103 64-65 2.3638638081272596 1103 66-67 2.5484555660548622 1103 68-69 2.590162508345802 1103 70-71 2.268753986487752 1103 72-73 2.394140894912809 1103 74-75 2.32091992644164 1103 76-77 2.2679663027527965 1103 78-79 2.460693737848988 1103 80-81 2.376992530418523 1103 82-83 2.221066476263328 1103 84-85 2.433223634905275 1103 86-87 2.3213791880893773 1103 88-89 2.2520460647522675 1103 90-91 2.329031662593117 1103 92-93 2.1276966964092594 1103 94-95 2.047146126695374 1103 96-97 1.903519222821366 1103 98-99 2.1045008933200045 1103 100-101 2.050637786484476 1103 102-103 1.9699368296610302 1103 104-105 1.9533746893199364 1103 106-107 2.0308529297672564 1103 108-109 2.003436896161478 1103 110-111 2.000962820391969 1103 112-113 1.9024749464106598 1103 114-115 1.771299767351902 1103 116-117 1.8409503830792282 1103 118-119 1.912578214062357 1103 120-121 1.9084996827457879 1103 122-123 1.996516255138097 1103 124-125 1.793480281900539 1104 1 0.6478983732717012 1104 2 0.6594111287916107 1104 3 0.7517669461129692 1104 4 0.6727711956946862 1104 5 0.9334837725309235 1104 6 1.040228603425252 1104 7 1.4638411997935918 1104 8 1.3391165566885626 1104 9 1.8015626077330928 1104 10-11 1.304020076495469 1104 12-13 1.4294988783959148 1104 14-15 1.5154576197569867 1104 16-17 1.3906545099550698 1104 18-19 1.3159268841775713 1104 20-21 1.6337231787123443 1104 22-23 1.6028775544520002 1104 24-25 1.9079943002584798 1104 26-27 1.6297307713259954 1104 28-29 1.5746592874918903 1104 30-31 2.056689050830272 1104 32-33 2.003233748627583 1104 34-35 1.9554900883943418 1104 36-37 1.8781994749594517 1104 38-39 2.0144750018758764 1104 40-41 2.2429259525106104 1104 42-43 2.19704260297841 1104 44-45 2.040588378181994 1104 46-47 2.019077104941722 1104 48-49 1.831137140592837 1104 50-51 2.1561326979632156 1104 52-53 2.0921414886230245 1104 54-55 2.3498373296344255 1104 56-57 2.3133650877310394 1104 58-59 2.3839443102917777 1104 60-61 2.4440561148834554 1104 62-63 2.5559255254880746 1104 64-65 2.6735912895667795 1104 66-67 2.6799426833979965 1104 68-69 2.802130185634077 1104 70-71 2.6184089723677246 1104 72-73 2.7602301598623527 1104 74-75 2.841275759522958 1104 76-77 2.7140451214414867 1104 78-79 2.6538404689466866 1104 80-81 2.5292381038021716 1104 82-83 2.4446376492687385 1104 84-85 2.8212798636879732 1104 86-87 2.7563695830666504 1104 88-89 2.4574603905052292 1104 90-91 2.565848822147238 1104 92-93 2.3938257338198667 1104 94-95 2.2631466356976873 1104 96-97 2.326631921044754 1104 98-99 2.334747929819091 1104 100-101 2.3433844502831214 1104 102-103 2.254929673812615 1104 104-105 2.2524051889940893 1104 106-107 2.3427132160863806 1104 108-109 2.293038660418869 1104 110-111 2.366031205598105 1104 112-113 2.2503898471871544 1104 114-115 2.1554960974239314 1104 116-117 2.129511887170416 1104 118-119 1.974719995698237 1104 120-121 1.9421496872181905 1104 122-123 2.2395119360724465 1104 124-125 2.059126033328514 1105 1 0.34111979532075765 1105 2 0.4405834279859384 1105 3 0.29286689840044744 1105 4 0.4144411728831159 1105 5 0.6459630959347429 1105 6 0.7534546423975677 1105 7 1.002577002457791 1105 8 0.9420343917508625 1105 9 1.5058403630450066 1105 10-11 1.130452685226544 1105 12-13 1.3032802583379386 1105 14-15 1.2034407549284794 1105 16-17 1.1478104981789983 1105 18-19 1.3385274681892199 1105 20-21 1.6314311752601185 1105 22-23 1.5163332308555368 1105 24-25 1.708007326500585 1105 26-27 1.5307415839703182 1105 28-29 1.640111405475757 1105 30-31 2.002276969816336 1105 32-33 1.7778086393210053 1105 34-35 1.9431190436044687 1105 36-37 1.5604841418421884 1105 38-39 1.7474643431289927 1105 40-41 1.8456944228747219 1105 42-43 1.6943264642199019 1105 44-45 1.6812048809737874 1105 46-47 1.5898807744352013 1105 48-49 1.5254958496285695 1105 50-51 1.6983797581821207 1105 52-53 1.6694349679150235 1105 54-55 1.8993027302841838 1105 56-57 1.541616632560256 1105 58-59 1.5953404698275193 1105 60-61 1.3850459970439921 1105 62-63 1.2765639494919299 1105 64-65 1.636425057342013 1105 66-67 2.0085132362556735 1105 68-69 2.006421755022128 1105 70-71 1.932294838528108 1105 72-73 2.1049058210034985 1105 74-75 1.9753495705060296 1105 76-77 1.8232674634216792 1105 78-79 1.9810535729793095 1105 80-81 1.9184648619698272 1105 82-83 1.887928546407725 1105 84-85 2.1302804034082428 1105 86-87 2.0683589276653613 1105 88-89 2.0095229453905503 1105 90-91 2.049443473552806 1105 92-93 1.8144519410001436 1105 94-95 1.7189503877518533 1105 96-97 1.7759612962135876 1105 98-99 1.8978322288157052 1105 100-101 1.9827086894216173 1105 102-103 1.7422640006802048 1105 104-105 1.3896016287181467 1105 106-107 1.548445118756284 1105 108-109 1.6418984757138766 1105 110-111 1.8587347292991225 1105 112-113 1.8711880611997849 1105 114-115 1.746997407980441 1105 116-117 1.759517452732183 1105 118-119 1.7853109888558443 1105 120-121 1.714617499611382 1105 122-123 1.816715461985872 1105 124-125 1.7242630069083376 1106 1 0.4979608961325823 1106 2 0.3454101873039157 1106 3 0.419558617499856 1106 4 0.39628850646543157 1106 5 0.5035429087143406 1106 6 0.5903743785073168 1106 7 0.9749580009644134 1106 8 0.564115160872035 1106 9 1.3574928408814309 1106 10-11 0.8904653967195344 1106 12-13 0.8669720217520442 1106 14-15 0.898742735773908 1106 16-17 0.9417564058572907 1106 18-19 0.9966709769578443 1106 20-21 0.8507602290285803 1106 22-23 0.8061999649585587 1106 24-25 0.5311051017401098 1106 26-27 0.7173192571841867 1106 28-29 0.7084725189253973 1106 30-31 1.2267812546361228 1106 32-33 0.9221075422992264 1106 34-35 0.9285178374428398 1106 36-37 1.0322843360684217 1106 38-39 1.1285235793147557 1106 40-41 1.2641561328609114 1106 42-43 1.2321413139350987 1106 44-45 1.1751949841685274 1106 46-47 1.0830569606904312 1106 48-49 1.1220121291050233 1106 50-51 1.4128223848931825 1106 52-53 1.0479445377774752 1106 54-55 1.6759125430323607 1106 56-57 1.4092122176892836 1106 58-59 1.2100949571784874 1106 60-61 1.560224011578562 1106 62-63 1.6154659287337978 1106 64-65 1.6163513107464702 1106 66-67 1.6823566090355904 1106 68-69 1.6239973226918103 1106 70-71 1.4390803509365995 1106 72-73 1.3385189978766867 1106 74-75 1.8194253722635167 1106 76-77 1.3423665033095666 1106 78-79 1.2135311992022544 1106 80-81 1.473601667083635 1106 82-83 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-4.550961132870114 2216 94-95 -4.272218892876829 2216 96-97 -4.4524043578950305 2216 98-99 -4.154649111064806 2216 100-101 -4.576073186704402 2216 102-103 -4.480318485925775 2216 104-105 -4.224895482803824 2216 106-107 -4.418561299343104 2216 108-109 -4.5580865864946 2216 110-111 -4.317643925839086 2216 112-113 -4.3215747454567115 2216 114-115 -4.1193396576797845 2216 116-117 -4.075158615350212 2216 118-119 -4.1721087303621545 2216 120-121 -4.119873133817638 2216 122-123 -4.28764879712088 2216 124-125 -4.16347721732215 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104050.0 3 34619.0 4 16200.0 5 13336.0 6 13489.0 7 15354.0 8 16931.0 9 17876.0 10 18797.0 11 20233.0 12 22123.0 13 23349.0 14 23459.0 15 19339.0 16 14550.0 17 12623.0 18 10939.0 19 10195.0 20 10165.0 21 10830.0 22 13201.0 23 18217.0 24 25107.0 25 33247.0 26 40616.0 27 45964.0 28 51713.0 29 59213.0 30 65297.0 31 73940.0 32 86499.0 33 102525.0 34 124416.0 35 157658.0 36 221475.0 37 287442.0 38 103884.0 39 527.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.396037866352835 19.877357614048126 14.260945420008877 24.46565909959016 2 15.673436471973748 20.69139051539758 39.12501624217024 24.510156770458433 3 19.22725505543473 24.87952447099564 29.263874666262417 26.629345807307214 4 12.93431058110566 17.343289703617877 36.673393897349726 33.049005817926734 5 13.101900692895423 38.50153501241107 34.166375339151635 14.230188955541875 6 31.57034458150888 37.55669290851378 16.988227262508534 13.884735247468798 7 27.551720989589 32.51704947274431 20.94460862168663 18.986620915980055 8 25.55684805284939 36.51158761636343 19.20400041662413 18.727563914163053 9 25.246494015153154 16.507957623963726 19.956244154113804 38.289304206769316 10-11 24.82305579224315 27.168802440983146 26.97943804277908 21.02870372399462 12-13 25.59019937743215 24.221674168177884 27.733428139922562 22.454698314467407 14-15 22.398870159039863 25.63367928439281 25.808863414644208 26.158587141923125 16-17 22.053190471782926 28.91915102947512 26.37257978145359 22.65507871728836 18-19 21.991989656421488 27.393028912776973 28.810501361913097 21.80448006888844 20-21 22.680038930820448 26.592612167815037 28.995649287449965 21.731699613914547 22-23 22.34390851656532 26.310536811615126 28.875548342436385 22.470006329383164 24-25 22.10400416231159 26.547318191498302 28.938739456929717 22.40993818926039 26-27 21.94760119402739 26.83081019677526 28.828624893583775 22.392963715613572 28-29 21.932495486859686 26.895248221428254 28.94718659005629 22.225069701655773 30-31 22.068751264900037 26.51846657376768 28.86008819817648 22.552693963155797 32-33 21.95411769740801 26.67308446903857 28.79224831223313 22.580549521320286 34-35 22.28992448078607 26.674535185064872 28.73496808764065 22.30057224650841 36-37 22.390804858479964 26.44637026831366 28.755880769651203 22.40694410355517 38-39 22.134115985528428 26.67379434290757 28.786135423614194 22.405954247949804 40-41 22.287879350386312 26.644968876458723 28.549893573576547 22.517258199578418 42-43 22.188637683759975 26.710226878682384 28.428039927100585 22.673095510457056 44-45 22.302594518086345 26.746288435335565 28.393956308145267 22.55716073843282 46-47 22.53209827495217 27.027364970167252 27.85053383902355 22.59000291585703 48-49 22.485262171695634 27.072048683173172 27.666614932373314 22.776074212757884 50-51 22.476289416515765 27.243887914121174 27.472785668911452 22.807037000451604 52-53 22.6989309824314 27.217911748365147 27.407996555631325 22.675160713572126 54-55 22.52422316889211 27.05136310911611 27.42444031319794 22.999973408793842 56-57 22.385632415494843 27.32684212873767 27.37417651446187 22.91334894130562 58-59 22.516368481353492 27.089411250295193 27.414280101351036 22.979940167000276 60-61 22.587060520842034 27.1095463643873 27.58593130445633 22.717461810314337 62-63 22.423975392243484 27.16871838726485 27.661631488482065 22.7456747320096 64-65 22.30942941815015 26.95861486137882 27.544664415818126 23.1872913046529 66-67 22.16653722031786 27.183294740179125 27.42849905604335 23.22166898345967 68-69 22.295023810622595 27.071766513326136 27.578452849656244 23.054756826395025 70-71 22.318961792195495 26.925498442255158 27.617769325884762 23.13777043966459 72-73 22.360222327483097 27.03911989523447 27.310321448141988 23.290336329140445 74-75 22.255854042255432 27.298366782670662 27.236849772160177 23.208929402913732 76-77 22.448868572960738 26.991747298729223 27.438182085373302 23.121202042936734 78-79 22.40535720086564 27.172005768880737 27.322105981784816 23.10053104846881 80-81 22.28222085639034 27.166628166707575 27.392961519978385 23.158189456923704 82-83 22.396022261598795 27.21386866564212 27.27863118031488 23.11147789244421 84-85 22.272360945180385 27.114484553704067 27.21008127784048 23.40307322327507 86-87 22.284907848863213 27.177411724426502 27.343288153447816 23.19439227326247 88-89 22.336978492051053 27.065148790126308 27.320024585227415 23.277848132595228 90-91 22.26284777320733 27.20282253334485 27.32889293941273 23.20543675403509 92-93 22.196257043921168 27.136496026115974 27.46838566230584 23.198861267657016 94-95 22.167308732525385 26.994250025653415 27.462096113417484 23.37634512840372 96-97 22.21903102054286 27.02383776091082 27.375087657784007 23.382043560762312 98-99 21.94430952285194 26.972338839165555 27.3105881309561 23.772763507026408 100-101 22.01271903624696 27.032347767439692 27.344020101305755 23.610913095007596 102-103 21.970224905220633 26.834427114956 27.494563318805437 23.70078466101793 104-105 21.967183107124765 26.85086476599047 27.461905050408696 23.72004707647607 106-107 21.628753259710813 26.833720266216698 27.553015820635018 23.98451065343747 108-109 21.909736938988285 26.790040002103744 27.249770289543456 24.050452769364515 110-111 21.687869557426982 26.824619501675134 27.479075331385133 24.00843560951275 112-113 21.798797151278027 26.886205808406327 27.46401456948452 23.850982470831124 114-115 21.744658832774267 26.963120171331912 27.2889436826513 24.003277313242517 116-117 21.538642403467467 26.796691550677064 27.502271323369413 24.16239472248605 118-119 21.460039414536354 26.80740193030165 27.44144280715649 24.291115848005504 120-121 21.411054586105298 26.856258617542505 27.583251777134492 24.149435019217698 122-123 21.480167207300855 26.825871447141342 27.39906743824988 24.29489390730792 124-125 21.36828692638469 26.746042427589533 27.347303632267412 24.53836701375836 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 226.0 1 197.0 2 288.0 3 438.0 4 363.5 5 287.0 6 478.0 7 1019.0 8 1629.5 9 1868.5 10 1546.0 11 1116.5 12 1054.0 13 1119.5 14 1258.0 15 1426.5 16 1562.0 17 1798.0 18 2067.5 19 2306.5 20 2700.0 21 3233.5 22 3942.0 23 4939.5 24 6256.5 25 8020.5 26 10446.0 27 13309.5 28 16134.5 29 19479.0 30 23521.5 31 27486.5 32 31303.0 33 34967.5 34 38579.0 35 41966.5 36 45868.0 37 50230.0 38 53406.5 39 55453.0 40 57133.5 41 59408.5 42 61919.5 43 64532.0 44 66698.5 45 68684.5 46 70575.5 47 71211.0 48 72138.5 49 72603.0 50 71218.5 51 69187.0 52 68045.0 53 67257.5 54 65300.0 55 60854.0 56 55380.5 57 51446.0 58 48142.5 59 43257.0 60 36800.5 61 30134.5 62 23762.5 63 18002.0 64 13161.0 65 9713.0 66 7278.5 67 5599.5 68 4387.0 69 3318.0 70 2505.0 71 1836.5 72 1329.5 73 1074.5 74 802.5 75 562.0 76 389.5 77 225.5 78 133.0 79 76.0 80 44.0 81 33.0 82 26.0 83 15.0 84 7.0 85 4.0 86 2.0 87 1.5 88 1.0 89 0.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10611540282087534 2 1.0312478408248333E-4 3 0.0 4 0.1796949362637272 5 0.0 6 1.0312478408248333E-4 7 0.03996085383196229 8 0.0 9 0.0 10-11 7.992170766392459E-4 12-13 5.671863124536583E-4 14-15 5.156239204124166E-4 16-17 0.0043570221274849205 18-19 0.0015726529572578705 20-21 0.003119524718495121 22-23 0.0015726529572578705 24-25 0.004692177675752991 26-27 0.004073428971258091 28-29 0.003145305914515741 30-31 7.992170766392459E-4 32-33 5.1562392041241666E-5 34-35 0.0012374974089897999 36-37 0.0013406221930722833 38-39 4.898427243917958E-4 40-41 0.0015468717612372497 42-43 6.960922925567625E-4 44-45 0.0010570290368454541 46-47 1.2890598010310416E-4 48-49 5.1562392041241666E-5 50-51 0.03916163675532304 52-53 2.5781196020620833E-5 54-55 0.13754268077001214 56-57 2.320307641855875E-4 58-59 0.0 60-61 0.0 62-63 4.898427243917958E-4 64-65 0.06012174912008778 66-67 0.052310046725839665 68-69 0.0016757777413403542 70-71 0.002887493954309533 72-73 0.06991860360792371 74-75 0.005001552028000442 76-77 0.001701558937360975 78-79 0.001392184585113525 80-81 1.0312478408248333E-4 82-83 2.320307641855875E-4 84-85 5.1562392041241666E-5 86-87 1.2890598010310416E-4 88-89 0.0011343726249073167 90-91 2.8359315622682917E-4 92-93 0.002500776014000221 94-95 0.004382803323505541 96-97 0.001959370897567183 98-99 0.0 100-101 0.0011085914288866958 102-103 0.0013148409970516624 104-105 2.5781196020620833E-5 106-107 2.5781196020620833E-5 108-109 0.0 110-111 2.5781196020620833E-5 112-113 1.0312478408248333E-4 114-115 2.320307641855875E-4 116-117 0.0 118-119 6.187487044948999E-4 120-121 0.0020624956816496665 122-123 4.64061528371175E-4 124-125 0.007270297277815074 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1939398.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.22426663274865 #Duplication Level Percentage of deduplicated Percentage of total 1 84.06751814491153 58.19512291216792 2 9.756254357451102 13.507391059542218 3 2.7473582524171163 5.705515806030145 4 1.1535946779713047 3.194269822960217 5 0.62667038296434 2.1690398840585092 6 0.35708650439768025 1.4831430834828714 7 0.25564258651215593 1.2387669399982097 8 0.16881405370284863 0.9348823251904914 9 0.1267414127894466 0.7896223217113119 >10 0.6870359013803478 8.512232074089425 >50 0.03713505547659279 1.7397972149510519 >100 0.015620809330826648 1.7975219268715992 >500 2.2622601197594145E-4 0.12058453924660177 >1k 3.016346826345874E-4 0.6121100896994148 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4242 0.21872766703894714 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3901 0.20114489135288371 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2467 0.12720442116574318 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.5781196020620833E-5 0.0 18-19 0.0 0.0 0.0 1.0312478408248333E-4 0.0 20-21 0.0 0.0 0.0 3.0937435224744997E-4 0.0 22-23 5.1562392041241666E-5 0.0 0.0 5.671863124536583E-4 0.0 24-25 5.1562392041241666E-5 0.0 0.0 8.507794686804875E-4 0.0 26-27 1.0312478408248333E-4 0.0 0.0 0.0011085914288866958 0.0 28-29 1.0312478408248333E-4 0.0 0.0 0.0012890598010310416 0.0 30-31 1.0312478408248333E-4 0.0 0.0 0.002578119602062083 0.0 32-33 1.0312478408248333E-4 0.0 0.0 0.005981237476784033 0.0 34-35 1.0312478408248333E-4 0.0 0.0 0.010312478408248333 0.0 36-37 1.0312478408248333E-4 0.0 0.0 0.01838199276270265 0.0 38-39 1.0312478408248333E-4 0.0 0.0 0.03186555828148735 0.0 40-41 1.0312478408248333E-4 0.0 0.0 0.04983505190786007 0.0 42-43 1.0312478408248333E-4 0.0 0.0 0.06764985835810905 0.0 44-45 1.0312478408248333E-4 0.0 0.0 0.0863927878651004 0.0 46-47 1.5468717612372498E-4 0.0 0.0 0.11382398043104097 0.0 48-49 1.5468717612372498E-4 0.0 0.0 0.14422001053935293 0.0 50-51 1.5468717612372498E-4 0.0 0.0 0.17706525426962388 0.0 52-53 1.5468717612372498E-4 0.0 0.0 0.2084151886306988 0.0 54-55 1.5468717612372498E-4 0.0 0.0 0.2440448015311968 0.0 56-57 1.5468717612372498E-4 0.0 0.0 0.28601658865276747 0.0 58-59 1.5468717612372498E-4 0.0 0.0 0.33739851232186485 0.0 60-61 1.5468717612372498E-4 0.0 0.0 0.3953288597801998 0.0 62-63 1.5468717612372498E-4 0.0 0.0 0.45199592863352445 0.0 64-65 1.5468717612372498E-4 0.0 0.0 0.5125043956939215 0.0 66-67 1.5468717612372498E-4 0.0 0.0 0.5848979941198248 0.0 68-69 1.5468717612372498E-4 0.0 0.0 0.6689446931470487 0.0 70-71 1.5468717612372498E-4 0.0 0.0 0.7647218363636552 0.0 72-73 1.5468717612372498E-4 0.0 0.0 0.868826305894922 0.0 74-75 1.5468717612372498E-4 0.0 0.0 0.9769784232014265 0.0 76-77 1.5468717612372498E-4 0.0 0.0 1.0951594257599524 0.0 78-79 1.5468717612372498E-4 0.0 0.0 1.2343005406832428 0.0 80-81 1.5468717612372498E-4 0.0 0.0 1.3904572449801433 0.0 82-83 1.5468717612372498E-4 0.0 0.0 1.5565654909410034 0.0 84-85 1.804683721443458E-4 0.0 0.0 1.722622174509822 0.0 86-87 2.0624956816496666E-4 0.0 0.0 1.9036319517706008 0.0 88-89 2.0624956816496666E-4 0.0 0.0 2.1044674687712375 0.0 90-91 3.0937435224744997E-4 0.0 2.5781196020620833E-5 2.337220106445402 0.0 92-93 3.0937435224744997E-4 0.0 5.1562392041241666E-5 2.588947704390744 0.0 94-95 3.0937435224744997E-4 0.0 5.1562392041241666E-5 2.843098734762024 0.0 96-97 3.0937435224744997E-4 0.0 5.1562392041241666E-5 3.0999567907154697 0.0 98-99 3.867179403093125E-4 0.0 5.1562392041241666E-5 3.382131981161164 0.0 100-101 4.382803323505542E-4 0.0 5.1562392041241666E-5 3.7149156593953383 0.0 102-103 4.64061528371175E-4 0.0 5.1562392041241666E-5 4.0824008274732675 0.0 104-105 5.671863124536583E-4 0.0 5.1562392041241666E-5 4.447385219537197 0.0 106-107 6.187487044948999E-4 0.0 5.1562392041241666E-5 4.80259853830931 0.0 108-109 6.187487044948999E-4 0.0 5.1562392041241666E-5 5.1807313403437565 0.0 110-111 6.187487044948999E-4 0.0 5.1562392041241666E-5 5.610246066047299 0.0 112-113 6.187487044948999E-4 0.0 5.1562392041241666E-5 6.069306042390474 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3765 0.0 49.93422 1 GTATCAA 6365 0.0 49.509464 1 ATCAACG 7835 0.0 37.742867 3 TATCAAC 8230 0.0 37.452682 2 TCAACGC 7980 0.0 36.53408 4 CAACGCA 7990 0.0 36.41588 5 AACGCAG 8255 0.0 34.814392 6 GTACATG 7030 0.0 30.648172 1 TACATGG 7050 0.0 29.8523 2 ACGCAGA 9850 0.0 28.995708 7 CGCAGAG 9910 0.0 28.924568 8 ACATGGG 7135 0.0 26.32139 3 GCAGAGT 10795 0.0 25.120932 9 GAGTACT 6440 0.0 24.196568 12-13 AGAGTAC 9860 0.0 23.766083 10-11 AGTACTT 6640 0.0 23.064684 12-13 GTACTTT 6940 0.0 22.796097 14-15 TATAACG 245 4.2652573E-7 21.901237 2 ACCGTCG 165 3.2275822E-4 21.625235 8 CAGAGTA 10665 0.0 21.35877 10-11 >>END_MODULE