FastQCFastQC Report
Thu 26 May 2016
SRR1364470_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364470_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3464264
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35060.10120475806693716No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG142050.036.752171
TACATGG144350.034.2105522
GTATCAA132300.031.6765171
ACATGGG148700.030.2897873
GGTATCA94150.025.98641
TATCAAC162600.025.9066662
CAACGCA154700.025.686535
ATCAACG153400.025.6769813
AACGCAG152200.025.157016
TCAACGC157650.025.0925034
CATGGGG79650.024.4586164
ACGCATA5250.023.8196437
GAGTACT51800.023.42586912-13
CATGGGA88850.022.1942484
ACGCAGA175200.021.5151757
CGCATAG5000.021.4382978
CGCAGAG174500.020.9537018
GTACTTT62850.020.15025714-15
AGTACAT108800.020.1241572
AGTACTT59750.019.7614112-13
Produced by FastQC (version 0.11.5)