##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364470_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3464264 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.069064309186597 31.0 27.0 31.0 10.0 34.0 2 27.184804044957314 31.0 27.0 31.0 10.0 34.0 3 27.245609168354374 31.0 27.0 31.0 10.0 34.0 4 30.108670413109394 35.0 30.0 37.0 10.0 37.0 5 30.743589691778688 35.0 30.0 37.0 16.0 37.0 6 30.879704606808257 35.0 31.0 37.0 13.0 37.0 7 30.66919351412017 35.0 30.0 37.0 11.0 37.0 8 30.29882335757321 35.0 30.0 37.0 10.0 37.0 9 30.97628009874536 35.0 29.0 39.0 10.0 39.0 10-11 31.108510061588838 36.0 29.5 39.0 10.0 39.0 12-13 30.959915150808367 36.0 29.5 39.0 8.0 39.0 14-15 31.677552143831996 37.0 30.0 40.0 8.0 41.0 16-17 31.950523545549647 37.0 30.0 40.0 8.0 41.0 18-19 31.893813231324174 37.0 30.0 40.0 8.0 41.0 20-21 31.670865730787263 37.0 29.5 40.0 7.0 41.0 22-23 31.72081717790561 37.0 30.0 40.0 4.5 41.0 24-25 31.731993144864248 37.0 30.0 40.0 2.0 41.0 26-27 31.839811140259517 37.0 30.0 40.0 2.0 41.0 28-29 31.76554110772158 37.0 30.0 40.0 2.0 41.0 30-31 31.40837924015029 37.0 30.0 40.0 2.0 41.0 32-33 31.419076028847687 37.0 30.0 40.0 2.0 41.0 34-35 31.11434506723506 36.5 29.5 40.0 2.0 41.0 36-37 30.870087412506667 36.0 29.0 40.0 2.0 41.0 38-39 30.716004611657773 36.0 28.5 40.0 2.0 41.0 40-41 30.725371247687818 36.0 28.5 40.0 2.0 41.0 42-43 30.58137413892244 36.0 28.0 40.0 2.0 41.0 44-45 30.215541165453903 36.0 27.5 40.0 2.0 41.0 46-47 29.94620863190565 35.0 27.0 40.0 2.0 41.0 48-49 29.68300741513926 35.0 26.5 40.0 2.0 41.0 50-51 29.648070268316733 35.0 26.5 40.0 2.0 41.0 52-53 29.43252203065355 35.0 26.0 39.5 2.0 41.0 54-55 29.284996322451175 35.0 26.0 39.0 2.0 41.0 56-57 28.838237357199105 34.5 25.0 39.0 2.0 40.5 58-59 28.574365579528582 34.0 25.0 39.0 2.0 40.0 60-61 28.124296243011504 34.0 23.5 38.5 2.0 40.0 62-63 28.053845636475742 34.0 23.5 38.0 2.0 40.0 64-65 27.844261147533793 33.5 24.0 38.0 2.0 40.0 66-67 27.573449944923368 33.0 23.0 37.0 2.0 40.0 68-69 27.383735621765545 33.0 23.5 37.0 2.0 39.5 70-71 27.230242845233505 33.0 23.5 36.0 2.0 39.0 72-73 27.202670899215533 33.0 24.0 36.0 2.0 39.0 74-75 26.929956839317093 33.0 23.5 36.0 2.0 39.0 76-77 26.63333452646796 33.0 23.0 35.0 2.0 37.5 78-79 26.339324312465795 33.0 22.5 35.0 2.0 37.0 80-81 26.359480686229453 33.0 23.5 35.0 2.0 37.0 82-83 26.18428070147079 33.0 23.0 35.0 2.0 36.0 84-85 26.0263496084594 33.0 23.0 35.0 2.0 36.0 86-87 25.83055318532306 33.0 22.5 35.0 2.0 36.0 88-89 25.62138321444324 33.0 20.5 35.0 2.0 35.0 90-91 25.469568572141153 33.0 20.0 35.0 2.0 35.0 92-93 25.375964707077753 33.0 20.0 35.0 2.0 35.0 94-95 25.328747174002906 33.0 20.0 35.0 2.0 35.0 96-97 25.2046789736579 33.0 19.0 35.0 2.0 35.0 98-99 25.129280562913216 33.0 18.5 35.0 2.0 35.0 100-101 25.068472552900126 33.0 18.0 35.0 2.0 35.0 102-103 23.873732342569735 30.5 14.5 34.0 2.0 35.0 104-105 24.679762858719776 32.0 15.0 35.0 2.0 35.0 106-107 24.766166493084825 32.0 14.0 35.0 2.0 35.0 108-109 24.70651875838562 32.0 10.5 35.0 2.0 35.0 110-111 24.600515145496995 32.0 7.5 35.0 2.0 35.0 112-113 24.58132376747269 32.0 7.0 35.0 2.0 35.0 114-115 24.519853712072752 32.0 2.0 35.0 2.0 35.0 116-117 24.309928891100675 32.0 2.0 35.0 2.0 35.0 118-119 24.14839558994349 32.0 2.0 35.0 2.0 35.0 120-121 23.996313935658485 32.0 2.0 35.0 2.0 35.0 122-123 23.70203613235019 32.0 2.0 35.0 2.0 35.0 124-125 22.665736935753166 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.0706511555257485 1101 2 1.692090588476205 1101 3 1.9874538805578261 1101 4 2.4089638096402552 1101 5 1.8820337924147843 1101 6 1.8566914552153015 1101 7 1.6225985593362502 1101 8 1.9830145961788794 1101 9 2.6110133896441994 1101 10-11 2.644832405922646 1101 12-13 2.418130585039993 1101 14-15 2.7394634765957484 1101 16-17 2.448894498830448 1101 18-19 2.4727669169332636 1101 20-21 2.822659525488337 1101 22-23 2.6650463549004506 1101 24-25 2.6158315552836804 1101 26-27 2.2945434400543547 1101 28-29 2.28372949836076 1101 30-31 2.319825083040339 1101 32-33 2.574109626267102 1101 34-35 2.8433505875395078 1101 36-37 2.816764206673657 1101 38-39 2.8576791248915754 1101 40-41 2.674733782014968 1101 42-43 2.698241834274018 1101 44-45 2.85544576540925 1101 46-47 2.803670007546671 1101 48-49 2.840718588310242 1101 50-51 2.7756178839811376 1101 52-53 2.599460039628795 1101 54-55 2.6487324483978334 1101 56-57 2.967253456200897 1101 58-59 2.941735500364299 1101 60-61 3.058375380917134 1101 62-63 2.6997761103816806 1101 64-65 2.6438841737182734 1101 66-67 2.659994012313689 1101 68-69 2.5318776537254166 1101 70-71 2.5062173235830656 1101 72-73 2.3015291466231993 1101 74-75 2.2711574243523636 1101 76-77 2.470309010457381 1101 78-79 2.420103661513199 1101 80-81 2.2742113086084395 1101 82-83 2.2949359599019914 1101 84-85 2.1433514198756605 1101 86-87 2.1519616852242045 1101 88-89 2.3154979259097956 1101 90-91 2.3338216516983614 1101 92-93 2.2599355724504164 1101 94-95 2.264777553683146 1101 96-97 2.300147655839833 1101 98-99 2.225654253760421 1101 100-101 2.1665442774372394 1101 102-103 2.144510903725493 1101 104-105 2.137812757367211 1101 106-107 2.3288768272298697 1101 108-109 2.305429284119352 1101 110-111 2.462133127856692 1101 112-113 2.3695249472414126 1101 114-115 2.332419726485494 1101 116-117 2.4030904598624723 1101 118-119 2.4449387602298316 1101 120-121 2.468222380372918 1101 122-123 2.4372535481462094 1101 124-125 2.4482476020974175 1102 1 1.1986523663313662 1102 2 0.9596266698323781 1102 3 1.115307280200419 1102 4 1.6512309780342633 1102 5 1.44316949467213 1102 6 1.3639148055280437 1102 7 1.366772123621221 1102 8 1.5031217281241283 1102 9 2.0626624327084997 1102 10-11 1.728654608631789 1102 12-13 1.9946071109566397 1102 14-15 2.3299602948330467 1102 16-17 1.842224215698451 1102 18-19 1.8738665951749844 1102 20-21 2.1447245465429354 1102 22-23 2.015577767179476 1102 24-25 1.7963962703805016 1102 26-27 1.8128271429108764 1102 28-29 1.5323317696689962 1102 30-31 1.6046845902288531 1102 32-33 1.4736446511415622 1102 34-35 1.9280996540213984 1102 36-37 2.1644498293196826 1102 38-39 2.105447162171707 1102 40-41 1.668189952465589 1102 42-43 1.644881650467159 1102 44-45 2.0911535656761586 1102 46-47 1.9517578917601242 1102 48-49 2.111156702579592 1102 50-51 1.9825878457512793 1102 52-53 2.0456179475401797 1102 54-55 1.819471119111089 1102 56-57 2.010245267405832 1102 58-59 2.021530379811832 1102 60-61 2.349884125136711 1102 62-63 2.165988623474437 1102 64-65 2.3095232885162993 1102 66-67 2.3612693154185997 1102 68-69 2.0958686315812116 1102 70-71 2.2860043169369426 1102 72-73 2.115800880739801 1102 74-75 2.259186702289167 1102 76-77 2.1714024520307724 1102 78-79 2.1258069283381182 1102 80-81 1.7156570631858443 1102 82-83 1.6661096470797716 1102 84-85 1.6871512725175606 1102 86-87 1.8196972691759896 1102 88-89 1.5858677624651953 1102 90-91 1.5927012586565858 1102 92-93 1.5000828753355933 1102 94-95 1.7385441870403824 1102 96-97 1.9025640851921857 1102 98-99 1.9438032842239608 1102 100-101 1.8896263992806404 1102 102-103 1.5845107210852625 1102 104-105 1.5800471921013646 1102 106-107 1.8533269739508569 1102 108-109 2.025361463713878 1102 110-111 1.8289319090092384 1102 112-113 1.7095867560932625 1102 114-115 1.7573423804717372 1102 116-117 2.001382582575186 1102 118-119 2.1252796348584972 1102 120-121 2.047751192647926 1102 122-123 2.1681782627106756 1102 124-125 1.9990185066875767 1103 1 1.5756133482144392 1103 2 1.2462274636674096 1103 3 1.496113961289229 1103 4 2.089753553341069 1103 5 1.4809717223663519 1103 6 1.490128522894853 1103 7 1.4500441224932707 1103 8 1.7455007551563888 1103 9 2.5501525524958453 1103 10-11 2.0560137185795035 1103 12-13 2.2193480535609744 1103 14-15 2.513559106216757 1103 16-17 2.3090274132557056 1103 18-19 1.9789155796671487 1103 20-21 2.343252056615416 1103 22-23 2.2464598583238136 1103 24-25 2.2700067386349367 1103 26-27 2.033077591169892 1103 28-29 1.8635178153195007 1103 30-31 2.0605969450876422 1103 32-33 2.0663253540003055 1103 34-35 1.9659216065365719 1103 36-37 2.337810141270932 1103 38-39 2.414719501421537 1103 40-41 2.42252855856875 1103 42-43 2.388236094818243 1103 44-45 2.3445239465432266 1103 46-47 2.5140216033590264 1103 48-49 2.5559236790992266 1103 50-51 2.2500511127228116 1103 52-53 2.288050096292391 1103 54-55 2.292837661827072 1103 56-57 2.452686744502966 1103 58-59 2.64231695867338 1103 60-61 2.5423559371570335 1103 62-63 2.3449347982121367 1103 64-65 2.245655350821096 1103 66-67 2.25816489919497 1103 68-69 2.2080811797005175 1103 70-71 2.426037194012146 1103 72-73 2.384654605170521 1103 74-75 2.3720184104016973 1103 76-77 2.3355858444436244 1103 78-79 2.4694835566327633 1103 80-81 2.039255247932644 1103 82-83 2.112520014502625 1103 84-85 2.0705607042575735 1103 86-87 2.1082925486062045 1103 88-89 2.0965788386998483 1103 90-91 2.1410844998220746 1103 92-93 2.1279396631663055 1103 94-95 1.9139417454351104 1103 96-97 2.015688247599382 1103 98-99 1.9722377885612872 1103 100-101 1.77391230379542 1103 102-103 1.8046493004139315 1103 104-105 2.0720816400413646 1103 106-107 1.8431671245715826 1103 108-109 2.0495512150672006 1103 110-111 2.1803462213962845 1103 112-113 2.06842436598615 1103 114-115 1.9852387603618205 1103 116-117 2.1413467076120405 1103 118-119 2.2221779601713543 1103 120-121 2.2568268533582234 1103 122-123 2.2769739662601474 1103 124-125 2.202666103178977 1104 1 1.538256200837786 1104 2 1.3634317053924256 1104 3 1.398343330536587 1104 4 1.7333298466377443 1104 5 1.3453722899400695 1104 6 1.2445581826602599 1104 7 1.253181326977323 1104 8 1.6672297010120651 1104 9 2.3135054780717965 1104 10-11 2.049867740665878 1104 12-13 1.5810617667396976 1104 14-15 2.002101716651982 1104 16-17 1.6992444401979583 1104 18-19 1.1233405410637864 1104 20-21 -3.648902778194522 1104 22-23 -0.21164004865117647 1104 24-25 1.2766368908539967 1104 26-27 1.3176950443309252 1104 28-29 0.9503909566703932 1104 30-31 1.8445394620939872 1104 32-33 1.6046055827529813 1104 34-35 1.8740131668392515 1104 36-37 1.9746099463357503 1104 38-39 1.9620219444386677 1104 40-41 2.0099705189059023 1104 42-43 2.0624846587194305 1104 44-45 2.2018230441602427 1104 46-47 2.1433089994799843 1104 48-49 2.188981381529583 1104 50-51 2.051875163524432 1104 52-53 2.0410772458634128 1104 54-55 1.9686629727069622 1104 56-57 2.2043164995819815 1104 58-59 2.225097268873416 1104 60-61 2.3802855047051708 1104 62-63 1.9452681602652042 1104 64-65 2.137573786331611 1104 66-67 2.0250775458843684 1104 68-69 1.991075407034252 1104 70-71 2.241637158202227 1104 72-73 2.0694046963781076 1104 74-75 2.0870606284086968 1104 76-77 2.0912387965404555 1104 78-79 2.0672832324587844 1104 80-81 1.874031489803052 1104 82-83 1.8932795886069407 1104 84-85 1.833671845584238 1104 86-87 1.7661798173730965 1104 88-89 1.926567355294246 1104 90-91 1.9398451199528992 1104 92-93 1.5718970762902345 1104 94-95 1.753871990140695 1104 96-97 1.8535502676825537 1104 98-99 1.7570611150871471 1104 100-101 1.254832988868742 1104 102-103 1.6242713509759206 1104 104-105 1.8025101743522463 1104 106-107 1.900967291774279 1104 108-109 1.963467944894841 1104 110-111 2.007893975804876 1104 112-113 1.9683595109961196 1104 114-115 1.8944216480681142 1104 116-117 1.9778837271230607 1104 118-119 1.9512315485000151 1104 120-121 1.9499176597679266 1104 122-123 1.7129710099226685 1104 124-125 1.8755126953807633 1105 1 1.2429026514938002 1105 2 0.9978616620301963 1105 3 0.9669751435266072 1105 4 1.6290364086510465 1105 5 1.1730846111772593 1105 6 1.1259125597842576 1105 7 0.9880757646967702 1105 8 1.3311636184715034 1105 9 1.7504376667882937 1105 10-11 1.5393751749652473 1105 12-13 1.6014092242841222 1105 14-15 1.8296304368780518 1105 16-17 1.5056401046797987 1105 18-19 1.2255886522671169 1105 20-21 1.37517382289845 1105 22-23 1.3529280461483388 1105 24-25 1.4101457592673405 1105 26-27 1.3721353021407694 1105 28-29 0.939856344678045 1105 30-31 1.3203641657167573 1105 32-33 1.2578196270411084 1105 34-35 1.2240200175726876 1105 36-37 1.4968155721499095 1105 38-39 1.374423188168656 1105 40-41 1.4570009326386995 1105 42-43 1.7653172235418602 1105 44-45 1.8994456634795611 1105 46-47 2.0825007105126545 1105 48-49 1.7260268411263873 1105 50-51 1.8172406945369097 1105 52-53 1.6902890659332002 1105 54-55 1.8421609951421303 1105 56-57 2.0758814090276196 1105 58-59 1.786532017094391 1105 60-61 2.0983310006223803 1105 62-63 1.9274163754424727 1105 64-65 1.643552084617557 1105 66-67 1.800803947242212 1105 68-69 1.3770181831493034 1105 70-71 1.450559421961021 1105 72-73 1.4308284536103422 1105 74-75 1.7159436254710805 1105 76-77 1.5052413907390587 1105 78-79 1.3338727815057005 1105 80-81 1.2762694570420479 1105 82-83 1.4741636435689287 1105 84-85 1.4363622729344812 1105 86-87 1.4425524576986142 1105 88-89 1.6023848897024067 1105 90-91 1.6437027537163509 1105 92-93 1.5891842007602683 1105 94-95 1.5333160155151084 1105 96-97 1.6108694733715225 1105 98-99 1.4222136635057723 1105 100-101 1.4750019749818826 1105 102-103 1.525156145898439 1105 104-105 1.6188105018322112 1105 106-107 1.6463384727783819 1105 108-109 1.7636943917887145 1105 110-111 1.646026479424716 1105 112-113 1.6849524278120889 1105 114-115 1.594660759670969 1105 116-117 1.7935861570124771 1105 118-119 1.867773081427174 1105 120-121 1.8244170344606268 1105 122-123 1.9293792754172543 1105 124-125 1.5796234539189058 1106 1 -0.32293424998578857 1106 2 -0.07089036295749906 1106 3 -0.13650468189369036 1106 4 -0.37118450222036614 1106 5 -0.7167330774114404 1106 6 -0.26110549314421405 1106 7 -0.13314068577426497 1106 8 0.3129941029213832 1106 9 0.3812940548670376 1106 10-11 -2.321249628032138 1106 12-13 -0.31180895761150396 1106 14-15 0.05550300905693106 1106 16-17 -0.13537174268332564 1106 18-19 -2.872372145619945 1106 20-21 -5.256375646846752 1106 22-23 -6.236980222424318 1106 24-25 -6.112678205100025 1106 26-27 -4.900181109967143 1106 28-29 -1.6222178239399803 1106 30-31 -3.392395434614219 1106 32-33 -4.029139541512336 1106 34-35 -3.318132377058568 1106 36-37 -5.66159079928924 1106 38-39 -6.145328273875897 1106 40-41 -2.250206422803629 1106 42-43 -0.8755826054179536 1106 44-45 -0.06498337518063124 1106 46-47 0.13572078789295006 1106 48-49 0.350702877446043 1106 50-51 0.15662147013158645 1106 52-53 0.002055141003459937 1106 54-55 0.0751170051956862 1106 56-57 0.2164915908924705 1106 58-59 0.3722729846530939 1106 60-61 0.5333491282984895 1106 62-63 0.24893809924940413 1106 64-65 -0.024297754249072767 1106 66-67 -0.3417318794103217 1106 68-69 -0.11903470533848903 1106 70-71 0.004116842711347601 1106 72-73 0.02684066028426102 1106 74-75 -0.07264173358276693 1106 76-77 -0.12561398398706203 1106 78-79 -0.09751379953462092 1106 80-81 -0.4556428341626564 1106 82-83 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-8.908561471686461 2216 96-97 -10.98841825441491 2216 98-99 -7.702300277261362 2216 100-101 -6.6832641322331305 2216 102-103 -7.564460756959871 2216 104-105 -10.435690697099384 2216 106-107 -7.084892569636608 2216 108-109 -9.454288356979951 2216 110-111 -6.860783527294473 2216 112-113 -6.244632807654394 2216 114-115 -6.536657497274788 2216 116-117 -6.0690720711111545 2216 118-119 -6.4902335651242105 2216 120-121 -7.288228957445995 2216 122-123 -10.092758124581604 2216 124-125 -8.546921030311644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 259427.0 3 43044.0 4 26087.0 5 22606.0 6 24073.0 7 26225.0 8 28568.0 9 30097.0 10 31696.0 11 32516.0 12 32472.0 13 32702.0 14 33085.0 15 28239.0 16 22850.0 17 20981.0 18 19416.0 19 18700.0 20 18879.0 21 21269.0 22 25936.0 23 35489.0 24 47105.0 25 59678.0 26 72440.0 27 83072.0 28 94569.0 29 108636.0 30 123469.0 31 140404.0 32 168091.0 33 205843.0 34 255414.0 35 325895.0 36 427332.0 37 420326.0 38 97472.0 39 161.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.39023023078554 18.038077274468776 13.545280185104605 24.026412309641078 2 15.470881864521646 20.249766758654186 38.962840488329846 25.31651088849432 3 18.774064639430012 24.81805955800623 28.99105899285811 27.416816809705647 4 11.47520483340409 16.163341102129344 36.79593481142619 35.56551925304037 5 14.356910898775546 37.847168069721846 32.87599741685353 14.919923614649075 6 32.84900445271962 35.71170625040594 17.021888328389874 14.417400968484559 7 29.358587361689363 32.23551785066334 20.245348654947026 18.16054613270027 8 26.21998409447478 34.859715288211866 19.697304287708146 19.222996329605206 9 26.420735512917403 16.446387301008116 19.809871925812466 37.323005260262015 10-11 25.002415526963993 26.450171903196946 26.863245067306185 21.684167502532873 12-13 25.745409732141518 23.942803663207535 27.197748427571284 23.114038177079664 14-15 23.132911506602742 25.219979488452694 24.780345289741845 26.866763715202723 16-17 23.52389131339536 28.442759488236412 24.856597551991914 23.17675164637631 18-19 23.27446542434827 27.20050201253963 26.65000028149426 22.875032281617834 20-21 24.086159842251583 26.49507787700965 26.507000924361346 22.91176135637743 22-23 23.7564809135589 26.33176936356299 26.379680162576253 23.532069560301856 24-25 23.623436313612046 26.529143101302914 26.36384632928861 23.48357425579643 26-27 23.647462040485816 26.835187977087532 26.09944566371324 23.417904318713415 28-29 23.430350813684754 26.676169807148604 26.459332505416967 23.434146873749683 30-31 23.4707639317279 26.36577827415704 26.488692761987842 23.674765032127215 32-33 23.469051799032382 26.599068927725344 26.126763297686928 23.805115975555346 34-35 23.89141266976334 26.330906047839992 26.178276211067157 23.59940507132951 36-37 23.864256838100967 26.182904469737306 26.062226368792913 23.890612323368813 38-39 23.62686383397438 26.339699344297646 26.32074724115548 23.71268958057249 40-41 23.692484008236338 26.260507295443592 26.422886536079897 23.624122160240173 42-43 23.70047346844506 26.448019516138345 26.04075004330504 23.810756972111555 44-45 23.579763995739278 26.484490727495817 25.976275890448804 23.959469386316098 46-47 23.549846332778266 26.682022082527517 25.800129700244632 23.96800188444958 48-49 23.968521576461622 26.558866153188838 25.532598528851373 23.94001374149817 50-51 23.74222603938494 26.682910458264015 25.550324599167844 24.024538903183203 52-53 24.094671976742678 26.579187061814075 25.345575955113596 23.980565006329652 54-55 23.725485328004538 26.29112867232919 25.645160172820248 24.33822582684603 56-57 23.65147563987889 26.71630668344653 25.62138187887757 24.010835797797014 58-59 24.0471946219518 26.22615523569668 25.503896099771794 24.222754042579727 60-61 24.05187659658086 26.110140310698803 25.817466004459344 24.02051708826099 62-63 23.95229520678693 26.44303390937533 25.538908996431164 24.065761887406584 64-65 23.79718475261253 26.32037342638104 25.54195915479357 24.34048266621286 66-67 23.650579335605872 26.708604121054236 25.42367596929302 24.217140574046873 68-69 23.438282150781493 26.50275432548327 25.797790188585125 24.26117333515011 70-71 23.370112517022612 26.37434805294528 25.99312816952536 24.26241126050675 72-73 23.79400193953271 26.383360373923665 25.56729311061759 24.25534457592604 74-75 23.581626999058518 26.766510080394617 25.50391869810978 24.147944222437083 76-77 23.829056004618938 26.371420323325633 25.69086316397229 24.10866050808314 78-79 23.699438320247104 26.45988159526924 25.855154500547616 23.985525583936038 80-81 23.679567503654557 26.77276333018491 25.56605222379515 23.981616942365385 82-83 23.67857793923067 26.6551379370021 25.58286792859154 24.083416195175687 84-85 23.723690175717085 26.502676754489844 25.425999512438235 24.34763355735483 86-87 23.802704299365747 26.249465778117898 25.698623659682223 24.249206262834136 88-89 23.734766674331052 26.207707137938286 25.697498711416284 24.36002747631438 90-91 23.6681158543811 26.507549021404976 25.807386541678856 24.016948582535065 92-93 23.827979312901032 26.343547174691444 25.491919029798616 24.33655448260891 94-95 23.693265680393264 26.235461444954332 25.59390453822596 24.477368336426444 96-97 23.696506939098107 26.149980471743785 25.581972345218002 24.571540243940106 98-99 23.476677832394003 26.062476112617965 25.61115614351712 24.84968991147091 100-101 23.470455388170897 26.228426871436834 25.767191712085108 24.53392602830716 102-103 23.784124172636346 25.951193067804308 25.854591834893725 24.410090924665614 104-105 23.789102299578726 26.083412754325792 25.8399175898049 24.28756735629058 106-107 23.455034975955744 26.268670417179884 25.93273785829318 24.343556748571185 108-109 23.525950323522068 26.24746154612228 25.56154857594 24.665039554415646 110-111 23.185741053518246 26.408752078051307 25.968590573881343 24.436916294549103 112-113 23.430998517947156 26.396966150652084 25.799670565114198 24.372364766286562 114-115 23.397943289190028 26.50497723554142 25.676987170382993 24.420092304885554 116-117 23.048671617953673 26.48590211116508 26.05531190437872 24.41011436650253 118-119 23.426344608115397 26.62733406232788 25.493442513160584 24.452878816396144 120-121 23.168172290346487 26.62858078188506 25.80439218905934 24.398854738709122 122-123 23.261394106326758 26.537406709295286 25.775968940890788 24.42523024348717 124-125 23.510224841232606 26.669904327273482 25.38626287464694 24.43360795684698 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2362.0 1 2245.0 2 2780.5 3 3190.0 4 2522.5 5 1852.0 6 1517.0 7 1460.0 8 1670.5 9 1812.5 10 1637.0 11 1490.5 12 1537.5 13 1634.0 14 1767.5 15 1949.5 16 2141.0 17 2296.5 18 2511.0 19 2794.5 20 3178.0 21 3805.5 22 4722.0 23 5786.5 24 6939.0 25 8803.5 26 11549.0 27 14784.0 28 18389.5 29 22351.5 30 26961.5 31 31942.5 32 36886.0 33 42507.5 34 49237.0 35 55061.5 36 60891.0 37 67520.0 38 72943.5 39 76265.5 40 79787.5 41 85906.0 42 92074.0 43 99286.5 44 107366.5 45 113081.5 46 116765.5 47 122865.0 48 134656.5 49 141824.5 50 141254.0 51 140075.5 52 140422.0 53 142195.5 54 141029.0 55 133280.5 56 123629.0 57 115251.0 58 108727.5 59 102014.0 60 89860.5 61 73119.5 62 57563.5 63 45108.0 64 35331.5 65 28188.0 66 21943.0 67 17210.0 68 13003.5 69 9466.0 70 7245.5 71 5702.5 72 4556.5 73 3758.0 74 2951.5 75 2239.5 76 1587.5 77 1021.5 78 696.0 79 452.5 80 309.5 81 217.5 82 159.5 83 121.0 84 100.5 85 70.0 86 56.5 87 44.0 88 31.5 89 31.5 90 24.5 91 18.5 92 12.5 93 12.0 94 9.0 95 7.0 96 5.5 97 4.0 98 3.5 99 3.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09531606136252896 2 0.032128036431403606 3 0.04200026325938208 4 0.048899275574840716 5 0.05253641177462225 6 0.00256908826809966 7 0.0014721741761020524 8 0.0017031034586278645 9 0.00513817653619932 10-11 0.06600247556190868 12-13 0.005657767421882397 14-15 0.010435116954135135 16-17 0.01941249281232608 18-19 0.017478460071172406 20-21 0.132149281925396 22-23 0.015457828849071549 24-25 0.014259883195968898 26-27 0.006105192906776157 28-29 0.004214459406096071 30-31 0.0019340327411536764 32-33 0.005585602021093081 34-35 0.01892176808695873 36-37 0.002583521348257523 38-39 0.007750564044772569 40-41 0.031031122339406005 42-43 0.013393898386497103 44-45 0.11021100008544384 46-47 0.0035361046386764984 48-49 0.008775312735980862 50-51 0.010868109358871033 52-53 0.011806259569132145 54-55 0.009179438980401031 56-57 0.005499003540145902 58-59 0.013841323871390863 60-61 0.03462495929871395 62-63 0.007259839319405218 64-65 0.006769114594037868 66-67 0.008544383453455049 68-69 0.00744746936145744 70-71 0.0035072384783607713 72-73 7.938194086824792E-4 74-75 0.0011402133324711974 76-77 0.0076206663233518 78-79 0.0183444448806442 80-81 0.0027711513903097454 82-83 0.004748483371937012 84-85 0.00252578902762607 86-87 0.0032763091958349596 88-89 0.005470137379830174 90-91 0.004026829364043849 92-93 0.0021938281839952154 94-95 0.003261876115677096 96-97 0.0015299064967335054 98-99 0.0026556867490468393 100-101 0.00252578902762607 102-103 6.494886071038466E-4 104-105 0.0037381677608865835 106-107 7.793863285246159E-4 108-109 0.0014144418554705993 110-111 0.0023381589855738477 112-113 0.004329924047358977 114-115 0.0026556867490468393 116-117 0.005239208097304363 118-119 0.0038103331616759 120-121 0.007245406239247355 122-123 0.004604152570358379 124-125 0.05448487759593379 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3464264.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.26470429069305 #Duplication Level Percentage of deduplicated Percentage of total 1 72.80368569744562 31.498299329725437 2 11.245726402049963 9.730860546374622 3 5.094404122602754 6.612236637050871 4 2.878172509558538 4.980931300946082 5 1.8323617199515332 3.9638293983644393 6 1.2925357735279293 3.355270681609684 7 0.8614538252198221 2.6089381505755385 8 0.6585241208994264 2.2792681087201823 9 0.4901869859391496 1.9087015494423079 >10 2.5989653440969733 19.710460466455125 >50 0.1508538310322503 4.492185653460936 >100 0.08781709220331865 6.8883906978629295 >500 0.004235702066438267 1.2224754723652411 >1k 0.0010768734062760325 0.7481520070466029 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3506 0.10120475806693716 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.4433080157863258E-5 0.0 12-13 0.0 0.0 0.0 1.1546464126290606E-4 0.0 14-15 0.0 0.0 0.0 1.7319696189435909E-4 0.0 16-17 0.0 0.0 0.0 2.1649620236794886E-4 0.0 18-19 0.0 0.0 0.0 3.4639392378871817E-4 0.0 20-21 0.0 0.0 0.0 5.484570459988037E-4 0.0 22-23 1.4433080157863258E-5 0.0 0.0 0.00128454413404983 0.0 24-25 2.8866160315726515E-5 0.0 0.0 0.0020494973824165827 0.0 26-27 2.8866160315726515E-5 0.0 0.0 0.0030453799133091475 0.0 28-29 2.8866160315726515E-5 0.0 0.0 0.004748483371937012 0.0 30-31 2.8866160315726515E-5 0.0 0.0 0.009785628347031288 0.0 32-33 2.8866160315726515E-5 0.0 0.0 0.019066098888537362 0.0 34-35 2.8866160315726515E-5 0.0 0.0 0.030554830694196518 0.0 36-37 2.8866160315726515E-5 0.0 0.0 0.04970752806368106 0.0 38-39 2.8866160315726515E-5 0.0 0.0 0.08128710744908588 0.0 40-41 2.8866160315726515E-5 0.0 0.0 0.11673475231679803 0.0 42-43 2.8866160315726515E-5 0.0 0.0 0.15192260174166866 0.0 44-45 2.8866160315726515E-5 0.0 0.0 0.1921764623019493 0.0 46-47 2.8866160315726515E-5 0.0 0.0 0.2425334789727342 0.0 48-49 2.8866160315726515E-5 0.0 0.0 0.2998328071994513 0.0 50-51 2.8866160315726515E-5 0.0 0.0 0.3608847362672129 0.0 52-53 2.8866160315726515E-5 0.0 1.4433080157863258E-5 0.4207820189223454 0.0 54-55 2.8866160315726515E-5 0.0 2.8866160315726515E-5 0.4838978784526814 0.0 56-57 2.8866160315726515E-5 0.0 2.8866160315726515E-5 0.5616921805035644 0.0 58-59 2.8866160315726515E-5 0.0 2.8866160315726515E-5 0.6514948052457896 0.0 60-61 2.8866160315726515E-5 0.0 2.8866160315726515E-5 0.7501737742851007 0.0 62-63 2.8866160315726515E-5 0.0 2.8866160315726515E-5 0.8509744061076177 0.0 64-65 2.8866160315726515E-5 0.0 2.8866160315726515E-5 0.9564657889814403 0.0 66-67 2.8866160315726515E-5 0.0 2.8866160315726515E-5 1.0813985308279046 0.0 68-69 2.8866160315726515E-5 0.0 2.8866160315726515E-5 1.2278509951897432 0.0 70-71 2.8866160315726515E-5 0.0 2.8866160315726515E-5 1.3944664725321165 0.0 72-73 2.8866160315726515E-5 0.0 2.8866160315726515E-5 1.5644592906314299 0.0 74-75 2.8866160315726515E-5 0.0 2.8866160315726515E-5 1.7432995868675136 0.0 76-77 2.8866160315726515E-5 0.0 2.8866160315726515E-5 1.938001838197089 0.0 78-79 2.8866160315726515E-5 0.0 2.8866160315726515E-5 2.164240369671595 0.0 80-81 2.8866160315726515E-5 0.0 2.8866160315726515E-5 2.416588343151677 0.0 82-83 2.8866160315726515E-5 0.0 2.8866160315726515E-5 2.6843364131601977 0.0 84-85 2.8866160315726515E-5 0.0 2.8866160315726515E-5 2.956515438777183 0.0 86-87 2.8866160315726515E-5 0.0 2.8866160315726515E-5 3.246995610034339 0.0 88-89 2.8866160315726515E-5 0.0 2.8866160315726515E-5 3.573789410968679 0.0 90-91 2.8866160315726515E-5 0.0 2.8866160315726515E-5 3.938296850355516 0.0 92-93 2.8866160315726515E-5 0.0 2.8866160315726515E-5 4.329664251916136 0.0 94-95 2.8866160315726515E-5 0.0 2.8866160315726515E-5 4.731712132793575 0.0 96-97 2.8866160315726515E-5 0.0 2.8866160315726515E-5 5.140485829024578 0.0 98-99 2.8866160315726515E-5 2.8866160315726515E-5 2.8866160315726515E-5 5.5761772197499955 0.0 100-101 2.8866160315726515E-5 2.8866160315726515E-5 2.8866160315726515E-5 6.0636978013223 0.0 102-103 2.8866160315726515E-5 2.8866160315726515E-5 4.329924047358977E-5 6.584197971055324 0.0 104-105 2.8866160315726515E-5 2.8866160315726515E-5 5.773232063145303E-5 7.108248678507181 0.0 106-107 2.8866160315726515E-5 2.8866160315726515E-5 5.773232063145303E-5 7.648103608731898 1.4433080157863258E-5 108-109 2.8866160315726515E-5 2.8866160315726515E-5 5.773232063145303E-5 8.213634988557455 2.8866160315726515E-5 110-111 2.8866160315726515E-5 2.8866160315726515E-5 5.773232063145303E-5 8.823735719910491 2.8866160315726515E-5 112-113 2.8866160315726515E-5 2.8866160315726515E-5 5.773232063145303E-5 9.472271743723919 2.8866160315726515E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14205 0.0 36.75217 1 TACATGG 14435 0.0 34.210552 2 GTATCAA 13230 0.0 31.676517 1 ACATGGG 14870 0.0 30.289787 3 GGTATCA 9415 0.0 25.9864 1 TATCAAC 16260 0.0 25.906666 2 CAACGCA 15470 0.0 25.68653 5 ATCAACG 15340 0.0 25.676981 3 AACGCAG 15220 0.0 25.15701 6 TCAACGC 15765 0.0 25.092503 4 CATGGGG 7965 0.0 24.458616 4 ACGCATA 525 0.0 23.819643 7 GAGTACT 5180 0.0 23.425869 12-13 CATGGGA 8885 0.0 22.194248 4 ACGCAGA 17520 0.0 21.515175 7 CGCATAG 500 0.0 21.438297 8 CGCAGAG 17450 0.0 20.953701 8 GTACTTT 6285 0.0 20.150257 14-15 AGTACAT 10880 0.0 20.124157 2 AGTACTT 5975 0.0 19.76141 12-13 >>END_MODULE