FastQCFastQC Report
Thu 26 May 2016
SRR1364470_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364470_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3464264
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100290.2894987218064212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89040.25702429145122885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56930.16433505067743104No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112000.040.5372661
GTATCAA166150.038.672581
GTACATG137000.032.399641
TACATGG136000.031.289662
ATCAACG205800.030.1182333
TATCAAC213250.030.1001192
TCAACGC208000.029.5417944
CAACGCA207200.029.486635
AACGCAG208500.028.7891986
ACATGGG138600.028.6386493
GAGTACT121550.027.64354512-13
GTACTTT132050.025.94085714-15
CATGGGG75600.025.4637454
AGTACTT128350.025.391312-13
CGCAGAG242450.025.1413968
ACGCAGA238350.025.0589587
GCAGAGT247450.023.8162789
AGAGTAC207250.022.84119210-11
ATGGGGA45900.022.551795
TACTTTT147450.022.2030614-15
Produced by FastQC (version 0.11.5)