##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364470_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3464264 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.988508670239913 31.0 28.0 31.0 25.0 34.0 2 29.199441786191816 31.0 28.0 34.0 25.0 34.0 3 29.251957991654216 31.0 28.0 34.0 25.0 34.0 4 32.71388381485938 35.0 32.0 37.0 26.0 37.0 5 31.799448887267253 35.0 32.0 37.0 22.0 37.0 6 31.82272771359227 35.0 32.0 37.0 22.0 37.0 7 31.29101708183903 35.0 31.0 37.0 17.0 37.0 8 31.400985894839422 35.0 32.0 37.0 17.0 37.0 9 31.99625952294629 37.0 30.0 39.0 11.0 39.0 10-11 32.18240815365111 37.0 31.0 39.0 16.5 39.0 12-13 31.917139975475312 37.0 30.5 39.0 12.5 39.0 14-15 32.86438966545275 38.0 31.0 40.0 13.5 41.0 16-17 32.9138838725917 38.0 31.0 40.0 13.0 41.0 18-19 32.75846254788895 38.0 31.0 40.0 10.5 41.0 20-21 32.62453525481892 38.0 30.5 40.0 10.5 41.0 22-23 32.5787923495438 38.0 31.0 40.0 10.0 41.0 24-25 32.35360440197398 37.0 30.0 40.0 10.0 41.0 26-27 32.432251843393 37.0 30.0 40.0 10.0 41.0 28-29 32.30704429570032 37.0 30.0 40.0 9.0 41.0 30-31 31.763185196047413 37.0 30.0 40.0 8.0 41.0 32-33 31.685214666087802 37.0 30.0 40.0 7.5 41.0 34-35 31.458497966667668 37.0 30.0 40.0 2.0 41.0 36-37 31.4357905459861 36.5 30.0 40.0 2.0 41.0 38-39 31.180899030789803 36.0 30.0 40.0 2.0 41.0 40-41 30.88237270600624 36.0 28.5 40.0 2.0 41.0 42-43 30.7475222731293 36.0 28.5 40.0 2.0 41.0 44-45 30.654043254209263 36.0 28.0 40.0 2.0 41.0 46-47 30.47598840619537 36.0 28.0 40.0 2.0 41.0 48-49 30.181429735147205 36.0 28.0 40.0 2.0 41.0 50-51 29.800091880988283 35.0 27.0 40.0 2.0 41.0 52-53 29.685580977662212 35.0 27.0 40.0 2.0 41.0 54-55 29.18613304297825 35.0 26.0 39.0 2.0 41.0 56-57 29.175447512083373 35.0 26.0 39.0 2.0 41.0 58-59 28.89967363919147 34.5 26.0 39.0 2.0 41.0 60-61 28.58183801811871 34.0 25.5 39.0 2.0 40.0 62-63 28.287616648153836 34.0 24.5 38.0 2.0 40.0 64-65 27.779083522502905 34.0 23.0 38.0 2.0 40.0 66-67 27.368852806829963 33.5 22.0 37.0 2.0 40.0 68-69 27.169226854535335 33.0 22.0 37.0 2.0 39.5 70-71 27.23819561673129 33.0 23.5 36.0 2.0 39.0 72-73 27.01997798089291 33.5 22.5 36.0 2.0 39.0 74-75 26.664115234866628 33.0 21.5 35.5 2.0 39.0 76-77 26.597647725462032 33.0 22.5 35.0 2.0 37.5 78-79 26.215889002685707 33.0 21.0 35.0 2.0 37.0 80-81 26.010459797521207 33.0 20.0 35.0 2.0 36.5 82-83 25.905426809273195 33.0 20.0 35.0 2.0 36.0 84-85 25.504364707770538 33.0 19.0 35.0 2.0 36.0 86-87 25.397076839409465 33.0 19.0 35.0 2.0 35.5 88-89 25.3853586216293 33.0 19.5 35.0 2.0 35.0 90-91 25.245010195527826 32.0 18.5 35.0 2.0 35.0 92-93 25.2819354991421 33.0 19.0 35.0 2.0 35.0 94-95 25.314487435137735 33.0 19.5 35.0 2.0 35.0 96-97 25.203924556558043 33.0 19.0 35.0 2.0 35.0 98-99 25.073797349162767 33.0 18.0 35.0 2.0 35.0 100-101 24.982260589839573 32.5 17.5 35.0 2.0 35.0 102-103 23.8418115651694 30.5 15.0 34.0 2.0 35.0 104-105 24.801482796923096 32.0 17.5 35.0 2.0 35.0 106-107 24.90068121251729 32.5 17.0 35.0 2.0 35.0 108-109 24.97536114453171 33.0 17.0 35.0 2.0 35.0 110-111 24.90511447741858 33.0 15.0 35.0 2.0 35.0 112-113 24.860976241995413 33.0 12.5 35.0 2.0 35.0 114-115 24.79878785219602 33.0 10.0 35.0 2.0 35.0 116-117 24.71478400606882 33.0 4.5 35.0 2.0 35.0 118-119 24.58955249946309 33.0 2.0 35.0 2.0 35.0 120-121 24.42625706354943 33.0 2.0 35.0 2.0 35.0 122-123 24.131148059154846 33.0 2.0 35.0 2.0 35.0 124-125 23.32847525477273 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8600915240581948 1101 2 0.8683635673059804 1101 3 0.8037274351874331 1101 4 0.8878649933225233 1101 5 1.0299627496411112 1101 6 1.0760138893441038 1101 7 1.639436474945306 1101 8 1.3768038021066573 1101 9 2.188240935225565 1101 10-11 1.7900251408498775 1101 12-13 1.9304483644143247 1101 14-15 1.8782254528001303 1101 16-17 1.8625002963050434 1101 18-19 1.842437630353686 1101 20-21 2.141397124412144 1101 22-23 2.1783618439646304 1101 24-25 2.3080026563157077 1101 26-27 1.9507102459002326 1101 28-29 2.059135814342916 1101 30-31 2.4435932993406766 1101 32-33 2.2704426553679937 1101 34-35 2.351864117774678 1101 36-37 2.173653248659434 1101 38-39 2.3228926089819133 1101 40-41 2.547067033252457 1101 42-43 2.5200759380226394 1101 44-45 2.3421517377123457 1101 46-47 2.027898274675426 1101 48-49 2.064167237803982 1101 50-51 2.2875536518088744 1101 52-53 2.191193233601279 1101 54-55 2.43093834076965 1101 56-57 2.155010846227487 1101 58-59 2.3215450590924647 1101 60-61 2.387370798837665 1101 62-63 2.3004184949006614 1101 64-65 2.42608656814987 1101 66-67 2.6197175330649145 1101 68-69 2.5500411397310536 1101 70-71 2.1380992066544344 1101 72-73 2.3014333229409196 1101 74-75 2.471682324810086 1101 76-77 2.2140838307936725 1101 78-79 2.2361903519875916 1101 80-81 2.349233620971436 1101 82-83 2.211664656164537 1101 84-85 2.463710597085644 1101 86-87 2.3065278279628885 1101 88-89 2.154361009979837 1101 90-91 2.191052001164877 1101 92-93 2.0964334860956306 1101 94-95 2.0249388453613477 1101 96-97 1.9652511557645944 1101 98-99 2.0554529038389155 1101 100-101 2.120005728207989 1101 102-103 1.9566044875424673 1101 104-105 2.004372742452123 1101 106-107 2.048287252521124 1101 108-109 2.0608834173158996 1101 110-111 2.0898902361394995 1101 112-113 1.9977091094899038 1101 114-115 1.9612082246271285 1101 116-117 2.0290093110927714 1101 118-119 1.9364288932992544 1101 120-121 1.8927201853406572 1101 122-123 1.9590269223370598 1101 124-125 1.7748026237495438 1102 1 0.5456221420645981 1102 2 -1.683070186817229 1102 3 0.051736444306136775 1102 4 0.431242189571293 1102 5 1.1112574734905003 1102 6 0.6231000272904339 1102 7 1.462022799354564 1102 8 1.0437117709689048 1102 9 1.8375466333459016 1102 10-11 1.5972257248455506 1102 12-13 1.7557977594220944 1102 14-15 1.7738541004723984 1102 16-17 1.686353351054919 1102 18-19 1.9164869171648249 1102 20-21 2.099242768216122 1102 22-23 1.9363600348352819 1102 24-25 2.1832963318951215 1102 26-27 1.8482044077840385 1102 28-29 1.8022396944425765 1102 30-31 2.1456056053279013 1102 32-33 1.8245514163508219 1102 34-35 2.0849099720821407 1102 36-37 1.5997199525526113 1102 38-39 1.467389488948772 1102 40-41 1.695940482828039 1102 42-43 1.5111668820970685 1102 44-45 1.5443205975247771 1102 46-47 1.2060037886264432 1102 48-49 1.307126740093782 1102 50-51 1.4660479841568979 1102 52-53 1.3120354075240463 1102 54-55 1.6073198512921323 1102 56-57 1.171090230166417 1102 58-59 1.1822215698315688 1102 60-61 1.142942104556738 1102 62-63 1.4810094733794124 1102 64-65 1.4927374390322896 1102 66-67 1.6393361731852245 1102 68-69 1.5649859243330972 1102 70-71 1.0462347812052037 1102 72-73 1.331924153693457 1102 74-75 1.5164228100327897 1102 76-77 1.440506746847408 1102 78-79 1.322171886069249 1102 80-81 1.4050661730104537 1102 82-83 1.0418992525848445 1102 84-85 1.0618363614491635 1102 86-87 1.1858246871455762 1102 88-89 1.282890497032625 1102 90-91 1.0652987871923614 1102 92-93 1.1340130938750264 1102 94-95 1.1640066029045997 1102 96-97 1.207359044888097 1102 98-99 1.0694685239676254 1102 100-101 0.8763637319417761 1102 102-103 0.6290208645096342 1102 104-105 0.9628013129907522 1102 106-107 1.2803359478044456 1102 108-109 1.1961777073593431 1102 110-111 1.018992236602191 1102 112-113 1.0170043772956987 1102 114-115 1.3339135412700713 1102 116-117 0.9779396693819358 1102 118-119 0.5412918522465446 1102 120-121 0.8661742065829934 1102 122-123 1.2239558144074465 1102 124-125 1.3703078547639507 1103 1 0.7587695541253652 1103 2 0.6570345042721364 1103 3 0.5634034266648804 1103 4 0.5466657177934522 1103 5 0.9934728058715372 1103 6 0.979319897092374 1103 7 1.3413556731541618 1103 8 1.1119338738769287 1103 9 1.8541316473241807 1103 10-11 1.5234604487906296 1103 12-13 1.4705322362728452 1103 14-15 1.2341703242444382 1103 16-17 1.1750741714935344 1103 18-19 1.4136376774605424 1103 20-21 1.544633284066471 1103 22-23 1.422983473049591 1103 24-25 1.8073853940902609 1103 26-27 1.3395711669745936 1103 28-29 1.4645461312854096 1103 30-31 2.0172529198327 1103 32-33 1.872576406345992 1103 34-35 1.952531464873676 1103 36-37 1.9290332439487443 1103 38-39 1.9319721843704762 1103 40-41 2.205625725956036 1103 42-43 2.1284732762146596 1103 44-45 1.9564755377735246 1103 46-47 1.9461094054294286 1103 48-49 1.9459784421962887 1103 50-51 2.029233742124088 1103 52-53 1.9747134827802917 1103 54-55 2.238503796284803 1103 56-57 2.055695790714495 1103 58-59 2.0682792546075675 1103 60-61 2.1404607648070204 1103 62-63 2.2571373130059342 1103 64-65 2.246487220065415 1103 66-67 2.492713461300056 1103 68-69 2.4574138361652835 1103 70-71 2.194686382760974 1103 72-73 2.32555257367121 1103 74-75 2.392045210023678 1103 76-77 2.193266797604995 1103 78-79 2.315341508135983 1103 80-81 2.262796130954573 1103 82-83 2.123528002971156 1103 84-85 2.297608784013221 1103 86-87 2.180121187520843 1103 88-89 2.0853875739138523 1103 90-91 2.1687273455999048 1103 92-93 1.9896565289276715 1103 94-95 1.9069306260275027 1103 96-97 1.7442393330271422 1103 98-99 2.017422376431661 1103 100-101 1.9372062951146098 1103 102-103 1.7670307368893319 1103 104-105 1.8334204801048521 1103 106-107 1.778499937259859 1103 108-109 1.7456449591719618 1103 110-111 1.7985327194828962 1103 112-113 1.697182676859473 1103 114-115 1.5764873598102653 1103 116-117 1.6598617285677904 1103 118-119 1.7523389625246715 1103 120-121 1.6712441976534151 1103 122-123 1.7101787390643253 1103 124-125 1.5479391957242115 1104 1 0.7447763580095277 1104 2 0.6475505978679479 1104 3 0.6378475315583216 1104 4 0.6369472295104259 1104 5 0.9419318351535502 1104 6 1.103538305274263 1104 7 1.3044078492797198 1104 8 1.1648593152072308 1104 9 1.6625669693643772 1104 10-11 1.174689832846596 1104 12-13 1.3699683983652235 1104 14-15 1.5060549624633168 1104 16-17 1.2818584679027225 1104 18-19 1.2270251003716197 1104 20-21 1.555113608132757 1104 22-23 1.5751463381660287 1104 24-25 1.8096692859550814 1104 26-27 1.667078668882887 1104 28-29 1.6390181706063132 1104 30-31 2.0220657187815547 1104 32-33 1.9922313051022087 1104 34-35 1.9482676279084252 1104 36-37 1.8493316778707012 1104 38-39 1.9979750437788617 1104 40-41 2.144832199742776 1104 42-43 2.0461646876234774 1104 44-45 1.6639090235458163 1104 46-47 1.8771940958823201 1104 48-49 1.6732714548199574 1104 50-51 1.9879071799164727 1104 52-53 1.8047729460892619 1104 54-55 2.1413135285047105 1104 56-57 1.9990494499898723 1104 58-59 2.13406227343739 1104 60-61 2.081868538789905 1104 62-63 2.079932815495802 1104 64-65 2.2686443367478546 1104 66-67 2.33558230680719 1104 68-69 2.4620269544416686 1104 70-71 2.183166729713296 1104 72-73 2.346114836481 1104 74-75 2.378359831104948 1104 76-77 2.239159359777606 1104 78-79 2.3206373034324983 1104 80-81 2.1968698388021934 1104 82-83 2.183840754128582 1104 84-85 2.4027335061834627 1104 86-87 2.2961399661872868 1104 88-89 2.2008659392046184 1104 90-91 2.2547889413490267 1104 92-93 2.0301308891619705 1104 94-95 1.9503888206422815 1104 96-97 1.967853549833304 1104 98-99 1.993601411206317 1104 100-101 2.094103843750805 1104 102-103 2.0336959451320737 1104 104-105 1.9046306196962135 1104 106-107 1.987847503464451 1104 108-109 1.924019438009779 1104 110-111 1.9346932973418696 1104 112-113 1.8936617610410522 1104 114-115 1.8025504162270352 1104 116-117 1.869449598160461 1104 118-119 1.8228470729322979 1104 120-121 1.8069964280323418 1104 122-123 1.956556990059532 1104 124-125 1.7665654007750256 1105 1 0.30047492140192134 1105 2 0.4711482100451505 1105 3 0.30764055402872614 1105 4 0.4780836679313438 1105 5 0.5982627259847071 1105 6 0.676023537234272 1105 7 0.894702887532695 1105 8 0.8611761278150709 1105 9 1.452732257030128 1105 10-11 1.0745424438230629 1105 12-13 1.2524981404415456 1105 14-15 1.1196644492451284 1105 16-17 1.0210152679773117 1105 18-19 1.2788666872914973 1105 20-21 1.4959007404631777 1105 22-23 1.4428013151730354 1105 24-25 1.6054377756170481 1105 26-27 1.3434977939080284 1105 28-29 1.4264511608111832 1105 30-31 1.7503934811788753 1105 32-33 1.616737320466278 1105 34-35 1.7313099146195476 1105 36-37 1.5032922894738263 1105 38-39 1.592663970337373 1105 40-41 1.6991750836264394 1105 42-43 1.7182726955684338 1105 44-45 1.612914528797905 1105 46-47 1.437537334567562 1105 48-49 1.5524446424452165 1105 50-51 1.709507671163525 1105 52-53 1.6334307837348767 1105 54-55 1.8724451449850683 1105 56-57 1.7183260111846081 1105 58-59 1.6051884648958072 1105 60-61 1.5246225973243064 1105 62-63 1.331763031960005 1105 64-65 1.6866901172652646 1105 66-67 1.914441363571651 1105 68-69 1.9462804657305384 1105 70-71 1.9067526800584211 1105 72-73 2.0174053642273506 1105 74-75 2.039713441163638 1105 76-77 1.7731690500079083 1105 78-79 1.9272886879718207 1105 80-81 1.8720825639006229 1105 82-83 1.8190450604819972 1105 84-85 2.0618457880103023 1105 86-87 2.013086430991997 1105 88-89 1.887465755542621 1105 90-91 1.9398100397106504 1105 92-93 1.738174551440732 1105 94-95 1.62722624020207 1105 96-97 1.6889688993792689 1105 98-99 1.7133017895348566 1105 100-101 1.67710454495149 1105 102-103 1.6179662681614353 1105 104-105 1.2297966500329665 1105 106-107 1.3923343543930322 1105 108-109 1.4144409132904912 1105 110-111 1.6057315812189152 1105 112-113 1.6672506793597073 1105 114-115 1.5905223850044976 1105 116-117 1.5530560641144149 1105 118-119 1.596276445733082 1105 120-121 1.6008415285450042 1105 122-123 1.7017630013036786 1105 124-125 1.5310782492544206 1106 1 0.3471891031997174 1106 2 0.32171016388243956 1106 3 0.41423796737574037 1106 4 0.3095954856152616 1106 5 0.4318266686038008 1106 6 0.6405906838338993 1106 7 0.9403316827958932 1106 8 0.5751244079461308 1106 9 1.17189490624029 1106 10-11 0.862875563951583 1106 12-13 0.7995674250434561 1106 14-15 0.9114893592250652 1106 16-17 0.9076949985340619 1106 18-19 0.9574343446208502 1106 20-21 0.7509790283080875 1106 22-23 0.7813285730532087 1106 24-25 0.6019818137922073 1106 26-27 0.5873946287028318 1106 28-29 0.5567550827812511 1106 30-31 0.9621750073002886 1106 32-33 0.7077428094710925 1106 34-35 0.723701353981486 1106 36-37 0.7428498968184414 1106 38-39 0.854665706571101 1106 40-41 1.1436690373120264 1106 42-43 1.122715518572349 1106 44-45 1.065231049454777 1106 46-47 0.9659397879389147 1106 48-49 0.9600126277573047 1106 50-51 1.2237461686150972 1106 52-53 1.0364001920276316 1106 54-55 1.4879634621409394 1106 56-57 1.1740547820998586 1106 58-59 1.1555881414748725 1106 60-61 1.413752356886988 1106 62-63 1.478114766723511 1106 64-65 1.6043087743305264 1106 66-67 1.6305727144071085 1106 68-69 1.4858633416239044 1106 70-71 1.4222974233895975 1106 72-73 1.3575797838545398 1106 74-75 1.5706541882717708 1106 76-77 1.257694691745229 1106 78-79 1.1984786298253063 1106 80-81 1.3352494117570295 1106 82-83 1.2245268619055523 1106 84-85 1.4968000401203412 1106 86-87 1.4925217964076438 1106 88-89 1.2648363136573977 1106 90-91 1.3450914205963045 1106 92-93 1.3324157784177046 1106 94-95 1.1767836489982741 1106 96-97 1.138193732715397 1106 98-99 1.132733248562019 1106 100-101 0.9236382778457575 1106 102-103 0.7458733879013799 1106 104-105 0.9586319389422044 1106 106-107 0.8945506252773718 1106 108-109 0.8146623153802963 1106 110-111 0.8487554900201992 1106 112-113 0.6168290181814058 1106 114-115 0.673598303871433 1106 116-117 1.0181085379177048 1106 118-119 0.7806555085900762 1106 120-121 0.8674578321589124 1106 122-123 0.991958354391457 1106 124-125 1.081439332556542 1107 1 0.2558809577717476 1107 2 0.3134828608074578 1107 3 0.16563040826926212 1107 4 0.20940725833269624 1107 5 0.14797008097367836 1107 6 0.40742897485597496 1107 7 0.5993768993923041 1107 8 0.5559223876992228 1107 9 1.2279900208432153 1107 10-11 0.7567488787174455 1107 12-13 1.0111824944390193 1107 14-15 0.8743685358383928 1107 16-17 0.8517812398581199 1107 18-19 0.9312487366580058 1107 20-21 1.1349662058799836 1107 22-23 0.916154517514201 1107 24-25 1.3548804660835003 1107 26-27 1.2641881807048065 1107 28-29 1.0479563119676243 1107 30-31 1.3962439232362627 1107 32-33 1.1954179734862507 1107 34-35 1.288208358149081 1107 36-37 1.0140974615040435 1107 38-39 1.144200802959812 1107 40-41 1.4889472366203158 1107 42-43 1.4446922161304876 1107 44-45 1.253367483125512 1107 46-47 1.3139286686497904 1107 48-49 1.0795178764815745 1107 50-51 1.4372998149289948 1107 52-53 0.9958694669833896 1107 54-55 1.5880386197291543 1107 56-57 1.3272608506349641 1107 58-59 1.3175511373448785 1107 60-61 1.5957199199863723 1107 62-63 1.518530046156929 1107 64-65 1.6872580353521265 1107 66-67 1.6557029368510072 1107 68-69 1.6328121691689468 1107 70-71 1.5015956528703107 1107 72-73 1.2099505724571138 1107 74-75 1.4301354624603952 1107 76-77 1.1675681801751985 1107 78-79 1.2377035751023087 1107 80-81 1.1601876977537877 1107 82-83 0.7065154663947695 1107 84-85 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-4.38730741448758 2216 102-103 -4.405270572261379 2216 104-105 -4.09875059662852 2216 106-107 -4.316804532484991 2216 108-109 -4.253708481248431 2216 110-111 -3.9378534506319447 2216 112-113 -4.050468361052211 2216 114-115 -4.004803031822934 2216 116-117 -3.9392367206035424 2216 118-119 -4.076808653160427 2216 120-121 -4.069456884646208 2216 122-123 -4.0957692600188835 2216 124-125 -4.00428668791082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 171819.0 3 59137.0 4 29669.0 5 24678.0 6 25539.0 7 30152.0 8 32596.0 9 34192.0 10 36433.0 11 38485.0 12 40975.0 13 43190.0 14 43516.0 15 35204.0 16 25509.0 17 22533.0 18 20088.0 19 18669.0 20 18630.0 21 20211.0 22 24703.0 23 33835.0 24 45906.0 25 60355.0 26 73586.0 27 84183.0 28 94700.0 29 106970.0 30 119442.0 31 136324.0 32 158685.0 33 189422.0 34 230088.0 35 295029.0 36 416283.0 37 489827.0 38 133326.0 39 375.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.19637845660205 18.944097943029927 13.868279246748564 24.991244353619464 2 16.127137328844825 20.57611826384555 37.42618179844781 25.870562608861817 3 19.080185574771438 24.35432172605783 29.286307279121914 27.27918542004882 4 13.239363061892792 16.808345989813894 35.234485159818675 34.71780578847464 5 14.189103370874736 37.69380740035979 33.026062678825866 15.091026549939613 6 32.9025721258972 36.00895430619282 16.446306637357814 14.642166930552166 7 28.387085036762887 32.36303906560662 20.240973736502152 19.008902161128336 8 26.78785450531484 34.459844861708 19.147876720711817 19.604423912265347 9 26.26197079668293 15.504996155027445 19.438068230365815 38.794964817923805 10-11 25.625635782319677 26.037920049326896 26.43608864984949 21.900355518503936 12-13 26.3892406977058 23.424015573980956 27.017011958901804 23.169731769411438 14-15 23.37044579699184 24.591895203716827 25.17008756597758 26.867571433313753 16-17 23.3338197492663 27.792998122030355 25.501488331718924 23.371693796984424 18-19 23.007612125130983 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23.676066012351082 44-45 23.49203384658995 25.4872454657168 27.105878698777413 23.91484198891583 46-47 23.445141884763103 25.71588985221931 26.83633277573389 24.0026354872837 48-49 23.915292215875674 25.734207497266738 26.32584857527396 24.024651711583633 50-51 23.63037275762091 25.807314227628193 26.31946610296751 24.242846911783392 52-53 23.980648692275583 25.8429501001945 26.059201065046466 24.11720014248345 54-55 23.575302708022203 25.515362783516192 26.26621251369967 24.64312199476193 56-57 23.65366210402601 25.93624292182584 26.211799725684443 24.198295248463705 58-59 23.936310858525793 25.526100204834272 26.15923613211926 24.378352804520674 60-61 23.949805788473398 25.391150905358252 26.50697233236266 24.152070973805692 62-63 23.84739576507646 25.66212175708279 26.254791091384494 24.235691386456256 64-65 23.666630754108834 25.5033270144562 26.253331419280652 24.57671081215431 66-67 23.520611603713903 25.906197639944622 26.074081946776374 24.4991088095651 68-69 23.3567611452902 25.667692640770746 26.483295144965453 24.492251068973605 70-71 23.443979060463207 25.59599868886134 26.452265762783494 24.507756487891957 72-73 23.644342454509307 25.59347006359013 26.136196716168662 24.6259907657319 74-75 23.451119930844463 25.934769608803272 26.04593924122035 24.568171219131916 76-77 23.66195349449453 25.549247847694357 26.271595223359444 24.51720343445167 78-79 23.553265790227634 25.758445474181325 26.26825904626094 24.4200296893301 80-81 23.33543094340494 25.911349032693852 26.220664730707277 24.532555293193926 82-83 23.380496885768 25.807828345298862 26.178456159574765 24.63321860935837 84-85 23.232881457453068 25.63180474920708 26.05309210834918 25.082221684990675 86-87 23.497735298201373 25.447203192145608 26.22689509109179 24.82816641856123 88-89 23.414485166297847 25.43451515296723 26.165202069391945 24.985797611342974 90-91 23.297487159547224 25.711027533544804 26.283099905982453 24.70838540092551 92-93 23.44006171756995 25.531632058784687 26.072483504334016 24.95582271931135 94-95 23.279870061168996 25.367574730632835 26.187154698425257 25.165400509772905 96-97 23.28757692121723 25.347228887665253 26.134255533961525 25.230938657155992 98-99 23.01019783711634 25.216395170806845 26.103625474271013 25.669781517805802 100-101 22.985202846187164 25.37282993672301 26.34665848395314 25.295308733136686 102-103 23.29330931439954 25.0210762247051 26.390911755820913 25.294702705074446 104-105 23.274681042809167 25.085718211518888 26.379674954925413 25.259925790746536 106-107 22.803053002342203 25.235894398287428 26.486651413778752 25.47440118559162 108-109 23.12048100260257 25.218054975025 25.985072153854325 25.676391868518106 110-111 22.622979780521575 25.311780284294606 26.429321022088853 25.63591891309497 112-113 22.87914754703131 25.238048571102382 26.281026995219612 25.601776886646693 114-115 22.790126257880832 25.305007506655823 26.18217921927893 25.722687016184416 116-117 22.55552694598333 25.17367325353957 26.45652871721093 25.81427108326617 118-119 22.614193075722568 25.31967046659555 26.066531639124 25.99960481855788 120-121 22.35757208537289 25.28321908347432 26.44236417680203 25.916844654350758 122-123 22.524933639951325 25.186941860789027 26.221164175575893 26.06696032368376 124-125 22.440697625913124 25.12911714487751 26.13165596879104 26.298529260418324 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 875.0 1 665.5 2 716.0 3 983.5 4 752.0 5 570.5 6 1066.5 7 2435.5 8 3946.0 9 4540.0 10 3628.5 11 2386.5 12 2067.5 13 2139.5 14 2347.0 15 2634.0 16 2885.5 17 3218.5 18 3656.0 19 3941.0 20 4366.5 21 4930.0 22 5644.0 23 6565.0 24 7506.0 25 8999.0 26 11323.5 27 14428.0 28 17888.5 29 21614.0 30 26094.0 31 30840.5 32 35538.0 33 40629.0 34 47087.0 35 53172.5 36 58823.0 37 65560.0 38 71360.0 39 74681.0 40 78278.0 41 84490.0 42 90614.0 43 98038.0 44 105969.5 45 111691.0 46 115341.5 47 120726.5 48 134488.0 49 143245.5 50 142710.0 51 142251.5 52 141974.0 53 143316.0 54 142631.5 55 134937.0 56 125046.0 57 116573.0 58 110246.5 59 103841.0 60 91791.5 61 74727.0 62 58820.5 63 45910.0 64 35986.0 65 28643.5 66 21727.5 67 16965.5 68 12870.0 69 9175.0 70 6799.5 71 5305.5 72 4342.0 73 3704.0 74 2933.0 75 2156.0 76 1466.5 77 891.0 78 547.0 79 321.0 80 204.0 81 136.5 82 102.5 83 78.5 84 60.5 85 41.5 86 24.5 87 17.5 88 13.0 89 9.0 90 8.5 91 5.0 92 1.0 93 1.5 94 1.5 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1050728235492445 2 2.886616031572652E-4 3 0.0 4 0.17954751716381892 5 0.0 6 2.5979544284153867E-4 7 0.04032602596106994 8 0.0 9 0.0 10-11 8.082524888403424E-4 12-13 7.072209277352997E-4 14-15 5.484570459988039E-4 16-17 0.00434435712751684 18-19 0.0015587726570492318 20-21 0.0031608445545720535 22-23 0.0018763004205222234 24-25 0.00505157805525214 26-27 0.004358790207674703 28-29 0.0034495061577293185 30-31 8.226855689982057E-4 32-33 1.1546464126290606E-4 34-35 0.0012556779737341035 36-37 0.0012556779737341035 38-39 5.628901261566671E-4 40-41 0.0015732057372070951 42-43 9.670163705768382E-4 44-45 0.0012989772142076932 46-47 2.5979544284153867E-4 48-49 4.329924047358977E-5 50-51 0.04110541228959456 52-53 5.773232063145303E-5 54-55 0.13808127787027777 56-57 1.5876388173649583E-4 58-59 0.0 60-61 0.0 62-63 6.350555269459833E-4 64-65 0.06209111083912773 66-67 0.05081887523583653 68-69 0.0022082612641530783 70-71 0.0028721829514147886 72-73 0.07171797530442253 74-75 0.0054412712195144475 76-77 0.0020783635427323094 78-79 0.001630938057838548 80-81 1.1546464126290606E-4 82-83 2.020631222100856E-4 84-85 4.329924047358977E-5 86-87 3.1752776347299165E-4 88-89 0.0016742372983121379 90-91 4.907247253673507E-4 92-93 0.0027855844704676087 94-95 0.004979412654462823 96-97 0.0024969228673103437 98-99 0.0 100-101 0.001457741095944189 102-103 0.001515473416575642 104-105 1.7319696189435909E-4 106-107 5.773232063145303E-5 108-109 0.0 110-111 1.5876388173649583E-4 112-113 7.21654007893163E-5 114-115 1.443308015786326E-4 116-117 0.0 118-119 9.381502102611117E-4 120-121 0.0021072297030480355 122-123 5.340239658409405E-4 124-125 0.0074619024416153045 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3464264.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.178660590618605 #Duplication Level Percentage of deduplicated Percentage of total 1 73.75648326255238 30.371931906262862 2 10.327036697281738 8.505070781284552 3 4.908876223257433 6.064228436366266 4 2.7717565160693765 4.565488832602254 5 1.8049276913869445 3.716225239711589 6 1.2476194659785187 3.0825179121466952 7 0.9107082496812582 2.625122213749061 8 0.6665195387691467 2.195710549119228 9 0.5466652390319866 2.025984810130881 >10 2.7668050387079046 20.389920541600855 >50 0.17607140593264745 5.010251499734551 >100 0.10921941270472332 8.37409177940771 >500 0.005863484657027107 1.6280317876112904 >1k 0.0012306078905609719 0.711354741636084 >5k 1.447773988895261E-4 0.43511754792415014 >10k+ 7.238869944476305E-5 0.29895142071187925 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10029 0.2894987218064212 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8904 0.25702429145122885 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5693 0.16433505067743104 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.8866160315726515E-5 0.0 10-11 0.0 0.0 0.0 4.329924047358977E-5 0.0 12-13 0.0 0.0 0.0 8.659848094717954E-5 0.0 14-15 0.0 0.0 0.0 1.1546464126290605E-4 0.0 16-17 0.0 0.0 0.0 1.4433080157863257E-4 0.0 18-19 0.0 0.0 0.0 2.4536236268367536E-4 0.0 20-21 0.0 0.0 0.0 4.47425484893761E-4 0.0 22-23 0.0 0.0 0.0 0.001010315611050428 0.0 24-25 0.0 0.0 0.0 0.0017608357792593173 0.0 26-27 0.0 0.0 0.0 0.0027134190696782926 0.0 28-29 0.0 0.0 0.0 0.004315490967201114 0.0 30-31 0.0 0.0 0.0 0.009092840499453853 0.0 32-33 0.0 0.0 0.0 0.01794031863622403 0.0 34-35 0.0 0.0 0.0 0.028548632552253525 0.0 36-37 0.0 0.0 0.0 0.04693637667337131 0.0 38-39 0.0 0.0 0.0 0.07698604956204261 0.0 40-41 0.0 0.0 0.0 0.11176977274249306 0.0 42-43 0.0 0.0 0.0 0.14669782672452214 0.0 44-45 0.0 0.0 0.0 0.18687952188401347 0.0 46-47 0.0 0.0 0.0 0.23629838834453726 0.0 48-49 1.4433080157863258E-5 0.0 0.0 0.29205337699436307 0.0 50-51 2.8866160315726515E-5 0.0 0.0 0.35154653340507536 0.0 52-53 2.8866160315726515E-5 0.0 0.0 0.4091633893952655 0.0 54-55 2.8866160315726515E-5 0.0 0.0 0.47038851542492144 0.0 56-57 2.8866160315726515E-5 0.0 0.0 0.5472302341853854 0.0 58-59 2.8866160315726515E-5 0.0 0.0 0.633511187369092 0.0 60-61 5.773232063145303E-5 0.0 0.0 0.7274705391967817 0.0 62-63 5.773232063145303E-5 0.0 0.0 0.8214587571847873 1.4433080157863258E-5 64-65 5.773232063145303E-5 0.0 0.0 0.9187665836091014 2.8866160315726515E-5 66-67 5.773232063145303E-5 0.0 0.0 1.0369735101020015 2.8866160315726515E-5 68-69 5.773232063145303E-5 0.0 0.0 1.1764403636674343 2.8866160315726515E-5 70-71 5.773232063145303E-5 0.0 0.0 1.335940332491981 5.773232063145303E-5 72-73 5.773232063145303E-5 0.0 0.0 1.496161955324421 5.773232063145303E-5 74-75 5.773232063145303E-5 0.0 0.0 1.6648702292896846 5.773232063145303E-5 76-77 5.773232063145303E-5 0.0 0.0 1.847520858687444 5.773232063145303E-5 78-79 5.773232063145303E-5 0.0 0.0 2.0583592936335107 5.773232063145303E-5 80-81 5.773232063145303E-5 0.0 0.0 2.293676232527313 5.773232063145303E-5 82-83 5.773232063145303E-5 0.0 0.0 2.544407701029714 5.773232063145303E-5 84-85 5.773232063145303E-5 0.0 0.0 2.802745402775308 5.773232063145303E-5 86-87 5.773232063145303E-5 0.0 0.0 3.0777533121032348 5.773232063145303E-5 88-89 5.773232063145303E-5 0.0 0.0 3.3881511339782415 5.773232063145303E-5 90-91 5.773232063145303E-5 0.0 0.0 3.7372873429969538 5.773232063145303E-5 92-93 5.773232063145303E-5 0.0 0.0 4.115664972415497 5.773232063145303E-5 94-95 5.773232063145303E-5 0.0 0.0 4.504131324864387 5.773232063145303E-5 96-97 5.773232063145303E-5 0.0 0.0 4.899063697223999 5.773232063145303E-5 98-99 5.773232063145303E-5 0.0 0.0 5.3266148307403824 5.773232063145303E-5 100-101 5.773232063145303E-5 0.0 0.0 5.808506511051121 5.773232063145303E-5 102-103 5.773232063145303E-5 0.0 0.0 6.327548939688199 8.659848094717954E-5 104-105 5.773232063145303E-5 0.0 0.0 6.849867677521113 8.659848094717954E-5 106-107 5.773232063145303E-5 0.0 0.0 7.39012673399025 8.659848094717954E-5 108-109 5.773232063145303E-5 0.0 0.0 7.9523385053794975 8.659848094717954E-5 110-111 5.773232063145303E-5 0.0 0.0 8.560346440109644 8.659848094717954E-5 112-113 5.773232063145303E-5 0.0 0.0 9.203498925024189 8.659848094717954E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11200 0.0 40.537266 1 GTATCAA 16615 0.0 38.67258 1 GTACATG 13700 0.0 32.39964 1 TACATGG 13600 0.0 31.28966 2 ATCAACG 20580 0.0 30.118233 3 TATCAAC 21325 0.0 30.100119 2 TCAACGC 20800 0.0 29.541794 4 CAACGCA 20720 0.0 29.48663 5 AACGCAG 20850 0.0 28.789198 6 ACATGGG 13860 0.0 28.638649 3 GAGTACT 12155 0.0 27.643545 12-13 GTACTTT 13205 0.0 25.940857 14-15 CATGGGG 7560 0.0 25.463745 4 AGTACTT 12835 0.0 25.3913 12-13 CGCAGAG 24245 0.0 25.141396 8 ACGCAGA 23835 0.0 25.058958 7 GCAGAGT 24745 0.0 23.816278 9 AGAGTAC 20725 0.0 22.841192 10-11 ATGGGGA 4590 0.0 22.55179 5 TACTTTT 14745 0.0 22.20306 14-15 >>END_MODULE