FastQCFastQC Report
Thu 26 May 2016
SRR1364469_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364469_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2269568
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26690.11759947267497603No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34350.052.8549731
GTATCAA62450.052.8068281
ATCAACG71550.043.409233
CAACGCA73700.042.2506265
TCAACGC73500.042.2035834
TATCAAC81700.040.2739562
AACGCAG76450.040.087456
GTACATG97450.036.100931
TACATGG97850.034.0524562
ACGCAGA88300.033.8996777
CGCAGAG87000.033.587628
ACATGGG99550.030.8410853
CGCATAG2751.8189894E-1228.133758
GCAGAGT99300.027.5093579
CATGGGG44450.024.2440834
GAGTACT47550.023.9578912-13
CATGGGA65000.023.9070554
AGAGTAC95500.022.67950610-11
CAGAGTA101350.022.57398210-11
GAGTACA76700.022.1964441
Produced by FastQC (version 0.11.5)