FastQCFastQC Report
Thu 26 May 2016
SRR1364469_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364469_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2269568
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62730.2763962128475551No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56390.24846138119677402No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37530.16536186622299928No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47650.066.349991
GTATCAA81000.048.7529451
ATCAACG99650.037.7395063
CAACGCA102000.036.3943025
TCAACGC102600.036.3058664
TATCAAC107950.036.1084482
AACGCAG104450.035.0849846
GTACATG94100.030.4284271
ACGCAGA124350.029.0396867
CGCAGAG125800.029.0251228
TACATGG95100.028.8917712
TGGTATC22750.026.7222182
GTGGTAT24400.025.9145641
GAGTACT85100.025.89476212-13
ACATGGG97900.025.8732153
GCAGAGT139100.025.608619
GTACTTT92250.024.30682814-15
CAGAGTA138900.023.8081610-11
AGTACTT89300.022.21251112-13
AGAGTAC133850.022.06247310-11
Produced by FastQC (version 0.11.5)