FastQCFastQC Report
Thu 26 May 2016
SRR1364468_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364468_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602755
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA71200.4442350827169468No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA58780.36674351351267037No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43580.2719068104607379No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT41500.2589291563588945No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT26950.16814797021378813No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG25590.1596625809933521No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA24170.15080283636613206No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC23070.14393965390842645No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20990.13096199980658305No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG18370.11461514704368415No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG90550.027.083881
TACATGG88800.026.4011382
GTATAGG5100.024.5104471
GAGTACT30750.023.60035912-13
CCCTATA5500.022.7193572
ACATGGG94200.022.5505283
AGTACTT34300.021.07104312-13
CATGGGG33550.020.5939144
TAAGGTG6400.019.543365
GTACTTT36800.019.11021414-15
CTCAACG4601.2551027E-1018.1277394
CATGGGA60050.017.8538934
GTATAGT4752.0008883E-1017.544321
TACCGTG1700.009663017517.5091027
GGATCGA2100.001623125417.0088426
GTATTAG5551.05501385E-1016.0879381
ACTTTTT48300.015.9881616-17
GTGTAGC9350.015.9158751
GTATAAA12000.015.8734321
TACTTTT44750.015.64862514-15
Produced by FastQC (version 0.11.5)