##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364468_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1602755 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.937410271688435 30.0 26.0 31.0 7.0 34.0 2 26.131571575193963 30.0 26.0 31.0 7.0 34.0 3 26.183752975345577 30.0 26.0 31.0 7.0 34.0 4 28.928758294311983 35.0 27.0 37.0 7.0 37.0 5 29.59409641523502 35.0 28.0 37.0 7.0 37.0 6 29.731731300167525 35.0 30.0 37.0 8.0 37.0 7 29.485404194652332 35.0 29.0 37.0 7.0 37.0 8 29.136339615225033 35.0 28.0 37.0 2.0 37.0 9 29.721742873988852 35.0 27.0 39.0 2.0 39.0 10-11 29.858406306640752 35.0 27.0 39.0 2.0 39.0 12-13 29.722821641486064 35.0 27.0 39.0 2.0 39.0 14-15 30.312114452926366 36.0 27.0 40.0 2.0 41.0 16-17 30.594380925344172 36.0 27.0 40.0 2.0 41.0 18-19 30.54591812223328 36.0 27.0 40.0 2.0 41.0 20-21 30.316203973782642 36.0 27.0 40.0 2.0 41.0 22-23 30.372230315924767 36.0 27.0 40.0 2.0 41.0 24-25 30.373120345904397 36.0 27.0 40.0 2.0 41.0 26-27 30.42786576862964 36.0 27.0 40.0 2.0 41.0 28-29 30.34102092958687 36.0 27.0 40.0 2.0 41.0 30-31 29.96534560803117 36.0 27.0 40.0 2.0 41.0 32-33 29.93683002080792 36.0 27.0 40.0 2.0 41.0 34-35 29.583893982548798 35.0 25.5 40.0 2.0 41.0 36-37 29.25095476226872 35.0 25.0 40.0 2.0 41.0 38-39 29.0287520550552 35.0 25.0 40.0 2.0 41.0 40-41 29.030077897120897 35.0 25.0 40.0 2.0 41.0 42-43 28.885894288272382 35.0 25.0 40.0 2.0 41.0 44-45 28.494583389226676 35.0 23.5 39.0 2.0 41.0 46-47 28.185150880827074 34.5 23.0 39.0 2.0 41.0 48-49 27.875901494613963 34.0 21.5 39.0 2.0 40.0 50-51 27.8056580700107 34.0 22.0 39.0 2.0 40.0 52-53 27.581580466134874 34.0 20.5 39.0 2.0 40.0 54-55 27.45774213775655 34.0 20.0 39.0 2.0 40.0 56-57 27.03114761769578 33.0 18.0 38.5 2.0 40.0 58-59 26.74524365857539 33.0 15.0 38.0 2.0 40.0 60-61 26.31904314758026 33.0 13.0 38.0 2.0 40.0 62-63 26.179917704203078 33.0 13.0 38.0 2.0 40.0 64-65 25.931383461602053 32.0 10.5 37.5 2.0 40.0 66-67 25.692326962012284 32.0 8.0 37.0 2.0 40.0 68-69 25.5194371566459 32.0 8.0 36.5 2.0 39.5 70-71 25.354208222716508 32.0 7.5 36.0 2.0 39.0 72-73 25.298288883828157 32.0 7.0 36.0 2.0 39.0 74-75 24.97534869646328 32.0 4.0 35.5 2.0 39.0 76-77 24.65551909056593 32.0 2.0 35.0 2.0 38.5 78-79 24.35367819785307 31.5 2.0 35.0 2.0 37.0 80-81 24.34033398741542 32.0 2.0 35.0 2.0 37.0 82-83 24.14241197188591 32.0 2.0 35.0 2.0 36.0 84-85 23.923862349516924 32.0 2.0 35.0 2.0 36.0 86-87 23.68018443243041 31.0 2.0 35.0 2.0 36.0 88-89 23.430180220932083 31.0 2.0 35.0 2.0 35.0 90-91 23.231558160791884 31.0 2.0 35.0 2.0 35.0 92-93 23.12274021918509 31.0 2.0 35.0 2.0 35.0 94-95 23.056116811365428 31.0 2.0 35.0 2.0 35.0 96-97 22.90102885344298 31.0 2.0 35.0 2.0 35.0 98-99 22.772200679455064 31.0 2.0 34.5 2.0 35.0 100-101 22.668264332352727 31.0 2.0 34.0 2.0 35.0 102-103 21.555094509142073 29.0 2.0 33.5 2.0 34.5 104-105 22.294186884458323 30.0 2.0 34.0 2.0 35.0 106-107 22.35346668704824 30.5 2.0 34.0 2.0 35.0 108-109 22.285591060392886 30.5 2.0 34.0 2.0 35.0 110-111 22.151643576217204 30.0 2.0 34.0 2.0 35.0 112-113 22.087125917560698 30.5 2.0 34.0 2.0 35.0 114-115 21.97146351126654 30.5 2.0 34.0 2.0 35.0 116-117 21.757064242507433 30.0 2.0 34.0 2.0 35.0 118-119 21.6090444266279 30.0 2.0 34.0 2.0 35.0 120-121 21.42020084167574 30.0 2.0 34.0 2.0 35.0 122-123 21.10050537979916 30.0 2.0 34.0 2.0 35.0 124-125 20.081725528855003 27.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6474511940424215 1101 2 1.4995566729023047 1101 3 1.7429854555239999 1101 4 2.075401274292453 1101 5 1.5348906153139872 1101 6 1.5599373926868978 1101 7 1.314691573994505 1101 8 1.6614810959326043 1101 9 2.2007820194688392 1101 10-11 2.1755184050245227 1101 12-13 1.9667638335164597 1101 14-15 2.418061976435755 1101 16-17 1.9571147834771168 1101 18-19 1.9398419093518413 1101 20-21 2.185364939159882 1101 22-23 2.1023131219214903 1101 24-25 2.0682178815753076 1101 26-27 1.641782175814381 1101 28-29 1.522983666709628 1101 30-31 1.6978486634736143 1101 32-33 1.9827367166584722 1101 34-35 2.289455750744512 1101 36-37 2.224679353360113 1101 38-39 2.252167774356785 1101 40-41 1.9644585448508103 1101 42-43 1.986195392368785 1101 44-45 2.2179571995470155 1101 46-47 2.2811248452713464 1101 48-49 2.195363002206637 1101 50-51 2.190526924614712 1101 52-53 2.0939872217548086 1101 54-55 2.143079321228196 1101 56-57 2.484659817585108 1101 58-59 2.469162865728279 1101 60-61 2.5531085172731025 1101 62-63 2.158549257571817 1101 64-65 2.063036099213498 1101 66-67 2.118451685214904 1101 68-69 2.015747638044978 1101 70-71 2.0261037056202973 1101 72-73 1.847861017632102 1101 74-75 1.7705681895349201 1101 76-77 2.0836364885762357 1101 78-79 2.094847265626804 1101 80-81 1.9600282092224646 1101 82-83 1.8780827008634162 1101 84-85 1.748667222285956 1101 86-87 1.752607598066362 1101 88-89 1.8457566725479282 1101 90-91 1.8079709735898462 1101 92-93 1.8486616413607706 1101 94-95 1.7968495554526136 1101 96-97 1.7103124060213304 1101 98-99 1.582155515582027 1101 100-101 1.6644256261887413 1101 102-103 1.715758367990862 1101 104-105 1.630193325415732 1101 106-107 1.672103259117371 1101 108-109 1.8082285655949093 1101 110-111 2.015226231836138 1101 112-113 1.8912761591015865 1101 114-115 1.85036200109246 1101 116-117 1.9413243125939914 1101 118-119 1.9156977744070716 1101 120-121 1.7079717645380015 1101 122-123 1.7992992863892958 1101 124-125 1.8363194420171496 1102 1 0.9642791779464197 1102 2 0.5882683322261357 1102 3 0.7441311431200646 1102 4 1.3997173337378435 1102 5 1.2240429914706503 1102 6 1.3145277036697323 1102 7 1.260100822588452 1102 8 1.2607888974426658 1102 9 1.6118105104467695 1102 10-11 1.1260904413799935 1102 12-13 1.4905692956452015 1102 14-15 1.8725983355744624 1102 16-17 1.3116901934744298 1102 18-19 1.4753789247211415 1102 20-21 1.557846195481794 1102 22-23 1.4987682288489097 1102 24-25 1.267087682195715 1102 26-27 1.3835701913114065 1102 28-29 1.0811182835340887 1102 30-31 0.9149840627833257 1102 32-33 0.86582116193809 1102 34-35 1.2492045186605587 1102 36-37 1.5284972354235826 1102 38-39 1.3273792142030487 1102 40-41 0.8043497309545771 1102 42-43 0.9116666085094138 1102 44-45 1.3012935669069563 1102 46-47 1.1729411946620232 1102 48-49 1.1983633119709687 1102 50-51 1.0425839212513495 1102 52-53 1.1408449489067571 1102 54-55 0.8725074463612401 1102 56-57 1.049258599374486 1102 58-59 1.1059899541050697 1102 60-61 1.4860835173832072 1102 62-63 1.1780448849033114 1102 64-65 1.4435012742156594 1102 66-67 1.2952873324828857 1102 68-69 1.0572199434990281 1102 70-71 1.3246997098072555 1102 72-73 1.1887588644029954 1102 74-75 1.3678874802186485 1102 76-77 1.2114527381376483 1102 78-79 1.1013021817041562 1102 80-81 0.6892287089560085 1102 82-83 0.5578633113780782 1102 84-85 0.7272680858090759 1102 86-87 0.7202167459502036 1102 88-89 0.5322074192854913 1102 90-91 0.5065541320996303 1102 92-93 0.4349013519733518 1102 94-95 0.5878632335296636 1102 96-97 0.7157097925499585 1102 98-99 0.7567232768961318 1102 100-101 0.6795430797204212 1102 102-103 0.4849718308477726 1102 104-105 0.443971451943451 1102 106-107 0.6856821675431064 1102 108-109 0.7436829282070896 1102 110-111 0.568356246237979 1102 112-113 0.4563980168654389 1102 114-115 0.5107022573894078 1102 116-117 0.5974943482535906 1102 118-119 0.7878150209398385 1102 120-121 0.6879949014376869 1102 122-123 0.8263618250180791 1102 124-125 0.6950020187278625 1103 1 1.3036071821919997 1103 2 0.9788808824857718 1103 3 1.1402002959572712 1103 4 1.6142584462125527 1103 5 1.1928829382534794 1103 6 1.00471016780158 1103 7 1.088430488949232 1103 8 1.317206467953575 1103 9 2.0578558924568604 1103 10-11 1.5034186004123526 1103 12-13 1.6974116084545443 1103 14-15 1.9675224347706752 1103 16-17 1.5852304988466344 1103 18-19 1.2371248459051856 1103 20-21 1.7532449741403866 1103 22-23 1.5432926464636445 1103 24-25 1.676097747021899 1103 26-27 1.4509437176122617 1103 28-29 1.1462488756672329 1103 30-31 1.4239937590695497 1103 32-33 1.489211287278529 1103 34-35 1.3634220530545527 1103 36-37 1.7807550954594795 1103 38-39 1.6875499956183155 1103 40-41 1.7493167650555783 1103 42-43 1.6725711529755998 1103 44-45 1.5502459920841325 1103 46-47 1.7656890654224284 1103 48-49 1.7471955931227292 1103 50-51 1.4774411672996806 1103 52-53 1.5203574442539356 1103 54-55 1.4313356367194991 1103 56-57 1.6207859395110695 1103 58-59 1.7657527617898658 1103 60-61 1.8117154712714871 1103 62-63 1.422543504721176 1103 64-65 1.3704994057180855 1103 66-67 1.3630504055164465 1103 68-69 1.337800467636555 1103 70-71 1.5478594976756561 1103 72-73 1.428008738178061 1103 74-75 1.44518062795526 1103 76-77 1.3795655555887514 1103 78-79 1.5680167585650082 1103 80-81 1.117971508606633 1103 82-83 1.267796937332136 1103 84-85 1.204061861906311 1103 86-87 1.2482342589688749 1103 88-89 1.3792298007169776 1103 90-91 1.3308877197255313 1103 92-93 1.3602605862947428 1103 94-95 1.1378954807757502 1103 96-97 1.186838740994908 1103 98-99 1.2069302120810086 1103 100-101 1.118940738017546 1103 102-103 1.0356519713352021 1103 104-105 1.323390706681952 1103 106-107 1.146350846437933 1103 108-109 1.3642216599136852 1103 110-111 1.5882474532431878 1103 112-113 1.4532355755093604 1103 114-115 1.3239571654202642 1103 116-117 1.4348624570171005 1103 118-119 1.6675762807568226 1103 120-121 1.613017658779924 1103 122-123 1.6386168841503128 1103 124-125 1.431692013973695 1104 1 1.0949263397175635 1104 2 0.8834007265841279 1104 3 0.9092163507989888 1104 4 1.3104731144519732 1104 5 0.837513954438684 1104 6 0.7886376641447903 1104 7 0.8011816371504139 1104 8 1.1869076237592004 1104 9 1.6275792658589623 1104 10-11 1.477830026845126 1104 12-13 1.0947447960068004 1104 14-15 1.2630676314594886 1104 16-17 1.013189767053678 1104 18-19 0.35400741574518335 1104 20-21 -3.980607634184416 1104 22-23 -0.8339224023484846 1104 24-25 0.4757578248045107 1104 26-27 0.6244325881383403 1104 28-29 0.3930262378287743 1104 30-31 1.1672562554162624 1104 32-33 0.912770560251488 1104 34-35 0.9578933057975796 1104 36-37 1.1257517558113008 1104 38-39 1.221794741016577 1104 40-41 1.2183994025996725 1104 42-43 1.1280440058061032 1104 44-45 1.4480292086173918 1104 46-47 1.330389720105611 1104 48-49 1.3824785380686428 1104 50-51 1.2824204320444181 1104 52-53 1.3723892329386622 1104 54-55 1.2288041623752157 1104 56-57 1.476871072855193 1104 58-59 1.368581965944344 1104 60-61 1.4615690944568485 1104 62-63 1.0638170706173788 1104 64-65 1.1794512748347188 1104 66-67 1.3229175765295622 1104 68-69 1.1677232947801492 1104 70-71 1.510202423824225 1104 72-73 1.3257127563390192 1104 74-75 1.2540064104597093 1104 76-77 1.2554900213676952 1104 78-79 1.165860390139784 1104 80-81 0.8526719600373553 1104 82-83 0.8408828072912122 1104 84-85 0.8569485724895252 1104 86-87 0.7438900530306789 1104 88-89 0.9739396998827274 1104 90-91 1.0094047056737736 1104 92-93 0.6961867902326553 1104 94-95 0.8663757060635824 1104 96-97 0.9651175010427622 1104 98-99 0.8325086230252445 1104 100-101 0.37665995105205496 1104 102-103 0.7935515183625661 1104 104-105 0.8076903652879075 1104 106-107 0.9551944310543838 1104 108-109 0.9106274905959744 1104 110-111 1.0311514543029041 1104 112-113 1.0287377667811377 1104 114-115 0.9417008248196019 1104 116-117 1.09033404828061 1104 118-119 1.0881616198064563 1104 120-121 1.0976989414010667 1104 122-123 0.8893573889313053 1104 124-125 0.983458943677082 1105 1 0.9053970738933543 1105 2 0.6120815604235439 1105 3 0.4681123935202507 1105 4 1.063286690550619 1105 5 0.7919861509504358 1105 6 0.8227532847005179 1105 7 0.6923775712741929 1105 8 0.9920789296708428 1105 9 1.3907275878466407 1105 10-11 1.0958755157388325 1105 12-13 1.196689263350791 1105 14-15 1.2864924396363655 1105 16-17 1.1225060983987376 1105 18-19 0.8069144033121098 1105 20-21 1.0175660554412147 1105 22-23 0.8693514638687674 1105 24-25 0.7672827145379699 1105 26-27 0.7516754116280353 1105 28-29 0.4309936468278224 1105 30-31 0.7959684830110518 1105 32-33 0.7212347813777384 1105 34-35 0.601027555880826 1105 36-37 0.9249825318249059 1105 38-39 0.8905170711582322 1105 40-41 0.9123914664038786 1105 42-43 1.0358823393029581 1105 44-45 1.2915182515249413 1105 46-47 1.6189868587250658 1105 48-49 1.068463234767595 1105 50-51 1.1345011186145086 1105 52-53 1.0733571347482886 1105 54-55 1.079666678861134 1105 56-57 1.4633395664993962 1105 58-59 1.0748117158874138 1105 60-61 1.494686357201303 1105 62-63 1.3275407814964275 1105 64-65 0.9301155053944896 1105 66-67 1.210792182900434 1105 68-69 0.8422665562288287 1105 70-71 1.034970039353034 1105 72-73 0.9651999142216141 1105 74-75 1.2155978373191907 1105 76-77 1.1889680113779555 1105 78-79 1.0077453143209993 1105 80-81 0.8992868580328448 1105 82-83 1.012150159059292 1105 84-85 0.9465546501414117 1105 86-87 0.9970508779348428 1105 88-89 1.0344331430946738 1105 90-91 1.0020599245601929 1105 92-93 0.9465305049662902 1105 94-95 0.9543878823058556 1105 96-97 1.0296478340866955 1105 98-99 0.8449486960132759 1105 100-101 0.9396201064560437 1105 102-103 0.9732448672952678 1105 104-105 1.0117986223774302 1105 106-107 1.1034476268001185 1105 108-109 1.1296970485486675 1105 110-111 1.0382560584917506 1105 112-113 1.1062242172895083 1105 114-115 0.9611270231114091 1105 116-117 1.0720264362256877 1105 118-119 1.0891451415139812 1105 120-121 0.999410034033918 1105 122-123 1.1021717796160253 1105 124-125 0.805873931053096 1106 1 -0.7101679863568222 1106 2 -0.5497601729950183 1106 3 -0.4708542529646884 1106 4 -0.8942161300586342 1106 5 -1.4550592599569008 1106 6 -0.8549445211172149 1106 7 -0.8838531715129321 1106 8 -0.5569134048790225 1106 9 -0.5364172400698664 1106 10-11 -3.04764210316927 1106 12-13 -1.0904485420745615 1106 14-15 -0.7719079124691497 1106 16-17 -0.9277690525566911 1106 18-19 -3.7064291233085136 1106 20-21 -6.260739322808945 1106 22-23 -6.905646649954733 1106 24-25 -6.833119722676159 1106 26-27 -5.5823308863982355 1106 28-29 -2.464850532921986 1106 30-31 -4.423136847576181 1106 32-33 -4.935570319824468 1106 34-35 -4.2472478296385034 1106 36-37 -6.634668394969378 1106 38-39 -7.003962642021765 1106 40-41 -3.151867573502713 1106 42-43 -1.707380259887202 1106 44-45 -1.1574698706842952 1106 46-47 -0.802023243246758 1106 48-49 -0.5462481773431414 1106 50-51 -0.677322517607756 1106 52-53 -0.7284396881947721 1106 54-55 -0.6643478664087574 1106 56-57 -0.3950180995926935 1106 58-59 -0.3170831060063115 1106 60-61 0.05462493492757403 1106 62-63 -0.284266279453977 1106 64-65 -0.4291670724079495 1106 66-67 -0.7691876584530064 1106 68-69 -0.5781964463650766 1106 70-71 -0.3831812249334021 1106 72-73 -0.3568765879430522 1106 74-75 -0.39101895638849626 1106 76-77 -0.37049718293139833 1106 78-79 -0.3975984148381926 1106 80-81 -0.7221551386858565 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>>Per sequence quality scores pass #Quality Count 2 155013.0 3 22989.0 4 13928.0 5 12993.0 6 14989.0 7 16632.0 8 17681.0 9 18097.0 10 19260.0 11 19473.0 12 19460.0 13 19772.0 14 20252.0 15 17554.0 16 14403.0 17 13075.0 18 12255.0 19 11729.0 20 11571.0 21 12428.0 22 14739.0 23 18769.0 24 23930.0 25 29089.0 26 34440.0 27 39305.0 28 44623.0 29 50259.0 30 56249.0 31 63933.0 32 74708.0 33 89271.0 34 108061.0 35 131353.0 36 162192.0 37 157981.0 38 40239.0 39 60.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.901202154122295 19.325771963560033 13.927975673381802 22.84505020893587 2 14.625836329139204 22.381353604953066 39.311151887357696 23.68165817855003 3 18.86806585471814 27.767041681023073 29.516401260367825 23.848491203890962 4 11.113850848160165 17.626275174195253 38.41559411163228 32.84427986601229 5 13.2035071181764 39.645292889314774 33.41076294490553 13.740437047603294 6 29.435856479242776 37.80020527923279 18.351339462592694 14.412598778931738 7 26.53842938130886 34.75684249013855 21.260917473323243 17.443810655229345 8 24.901604280744138 34.727075107692464 20.88060737050699 19.490713241056408 9 25.973932782820196 17.77020964342273 20.58590089016402 35.66995668359305 10-11 23.2174368443097 27.603145503244942 27.85644864993418 21.322969002511183 12-13 25.709662061525158 25.494577971570465 27.432425096513292 21.36333487039109 14-15 23.24877866753861 26.670412772619688 25.967565503293805 24.113243056547894 16-17 21.490302781621857 29.820783609508904 26.749563543264216 21.939350065605026 18-19 21.296280694922626 29.543478844225273 28.324044275450365 20.836196185401732 20-21 22.493877677256823 28.402079231088123 27.499577474600834 21.60446561705422 22-23 22.046264305681273 28.166049025049823 26.898222209325436 22.88946445994347 24-25 22.09585208422323 28.322608807164144 27.490835296176957 22.090703812435667 26-27 22.65981788582578 27.80548239415308 27.451187773029307 22.083511946991834 28-29 21.939422868436527 27.885260043022026 28.254207438536067 21.921109650005384 30-31 21.92042193389906 28.835529449861813 27.587803325044273 21.65624529119485 32-33 21.535161679299527 28.850251071284276 27.060258668160042 22.554328581256154 34-35 22.16338545332914 27.995651674679365 27.267176134633335 22.573786737358155 36-37 22.78055347140221 27.89260639451177 27.406677159360516 21.920162974725503 38-39 23.006582481949497 27.400197305980896 27.662273561435352 21.930946650634255 40-41 22.075545064399112 27.90285957523299 28.239928198547492 21.7816671618204 42-43 21.810260719203225 28.692341605463177 27.276811775530817 22.220585899802778 44-45 21.854863292132332 28.94314062058851 26.829434354488512 22.37256173279065 46-47 21.8993216040033 28.505422956271616 26.204242587794486 23.391012851930597 48-49 22.35160068801708 28.35156387230188 26.456521732347877 22.84031370733316 50-51 23.02373375907188 28.545844322340326 26.23986107128739 22.190560847300407 52-53 22.25193217851392 28.515525913547357 26.889441417807646 22.343100490131075 54-55 21.712453118170135 28.38606073445314 26.94702528725017 22.954460860126552 56-57 22.131884800860583 29.070495531261514 25.97212650303391 22.82549316484399 58-59 21.936962010466186 28.175238097615342 26.41594913927915 23.471850752639323 60-61 22.15953627935075 27.698921080616895 26.688994373243197 23.45254826678915 62-63 23.1341106643775 27.525387326569202 26.45039795759508 22.89010405145822 64-65 22.749996724136746 27.638520657593666 26.734288803848173 22.87719381442141 66-67 21.93340074523325 27.978039237220102 26.914445438324506 23.174114579222138 68-69 22.04402959183928 28.50694869100539 26.64092314757732 22.80809856957801 70-71 21.923186235955843 28.174104626128237 26.347432422862244 23.555276715053676 72-73 22.14230696878688 27.864447091994677 25.89126305304137 24.101982886177073 74-75 22.382237540544143 28.006989331006466 26.294690585460984 23.316082542988408 76-77 22.86420551174985 27.3278736448927 26.772399381128537 23.035521462228907 78-79 22.227110767040763 27.416342696560847 27.26322723214857 23.09331930424982 80-81 22.20111537338428 28.42548661876393 26.51342241678376 22.85997559106803 82-83 22.21520266855352 28.18731413781786 26.25241316656122 23.3450700270674 84-85 22.432679903724814 27.434715534278926 26.19620982749396 23.9363947345023 86-87 22.842874765158545 27.442959498389257 26.50861652098333 23.205549215468867 88-89 23.341508393814326 26.71956277101792 26.78167908733005 23.157249747837707 90-91 22.572045495883224 27.181409795835087 27.18559037045195 23.060954337829738 92-93 22.382223453480883 27.5857679809521 26.825205778263626 23.206802787303392 94-95 22.24969714667398 27.779613252480935 26.399513063640224 23.571176537204863 96-97 22.38490042870843 27.208728156678546 25.965311492143673 24.44105992246935 98-99 22.49524473286115 27.070034782006452 26.2775266558578 24.1571938292746 100-101 22.86453042755564 27.373506575187246 26.339215519887787 23.422747477369327 102-103 22.271231966457496 27.138638926714677 27.15192864730298 23.438200459524847 104-105 21.83972161959295 27.36290470013396 27.06060214675369 23.736771533519395 106-107 21.98445291325377 28.159643711035574 26.30607258551584 23.549830790194815 108-109 21.72764873132806 27.84564423777047 26.38283588282309 24.043871148078384 110-111 21.700705208497443 27.638120879069454 26.64309212322197 24.018081789211138 112-113 22.67865276503878 27.66533141156431 26.26827396439736 23.387741858999558 114-115 22.319061491358717 28.001925485099328 26.37852191877148 23.300491104770472 116-117 21.53836314542383 28.086686186085473 26.844046915240277 23.53090375325042 118-119 21.698809851010655 28.445836818341068 26.398508515481854 23.456844815166424 120-121 21.583932367038226 28.518132930417455 26.057053612125664 23.84088109041865 122-123 21.561299669803905 28.194578594407883 25.97826521011004 24.265856525678174 124-125 22.020809325054717 28.21656830295441 25.90858979273078 23.854032579260092 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2899.0 1 2269.5 2 1786.0 3 1693.0 4 1237.0 5 908.5 6 743.0 7 723.0 8 845.5 9 924.5 10 811.0 11 717.5 12 692.5 13 737.0 14 858.0 15 943.0 16 1043.0 17 1203.5 18 1400.0 19 1596.0 20 1896.5 21 2324.0 22 2869.5 23 3560.5 24 4529.5 25 5989.5 26 8080.0 27 10447.5 28 12887.5 29 15620.5 30 18826.5 31 22896.0 32 27220.5 33 30781.5 34 33948.5 35 36935.5 36 40244.5 37 43579.5 38 44945.5 39 45515.0 40 46355.0 41 47439.5 42 48399.0 43 49794.0 44 52309.5 45 54532.0 46 57191.0 47 62576.0 48 76961.0 49 78554.5 50 65844.5 51 59101.5 52 53807.0 53 50624.0 54 47983.5 55 44238.0 56 40307.0 57 36526.0 58 33226.0 59 29720.5 60 25344.5 61 20993.5 62 17178.5 63 13644.5 64 10313.0 65 8015.0 66 6539.5 67 5402.0 68 4469.0 69 3755.0 70 3136.0 71 2532.5 72 2114.5 73 1803.5 74 1478.0 75 1172.0 76 883.0 77 680.5 78 536.0 79 399.5 80 307.0 81 229.0 82 167.5 83 123.5 84 93.0 85 67.0 86 52.0 87 35.5 88 27.0 89 19.0 90 14.5 91 13.0 92 12.0 93 11.0 94 7.5 95 5.0 96 3.0 97 1.5 98 1.0 99 2.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09645890981466287 2 0.03213217241562185 3 0.04261412380557228 4 0.05097472789041369 5 0.054281533983671866 6 0.0037435540678394386 7 0.0030572358220688754 8 0.0026828804152849313 9 0.008048641245854794 10-11 0.04504743394966792 12-13 0.007861463542462822 14-15 0.012603298695059445 16-17 0.022617305826529942 18-19 0.02071433250871156 20-21 0.14097600693805354 22-23 0.01800025580952797 24-25 0.017189152428162758 26-27 0.008360604084841414 28-29 0.0054593496822658485 30-31 0.002464506427994297 32-33 0.007643089555172187 34-35 0.01865537777139987 36-37 0.003275609809359509 38-39 0.01048195138995043 40-41 0.034877445398704104 42-43 0.015317375394243037 44-45 0.11374165109452163 46-47 0.004835424004292608 48-49 0.01113707335182233 50-51 0.012884065250147401 52-53 0.014007131470499234 54-55 0.01176099902979557 56-57 0.007580696987374864 58-59 0.01628446019510156 60-61 0.025643345364700157 62-63 0.008391800368740076 64-65 0.007736678406868174 66-67 0.010575540241646414 68-69 0.00942127773739592 70-71 0.005396957114468525 72-73 9.670848008585218E-4 74-75 0.0016534030466290855 76-77 0.0100140071314705 78-79 0.02305405380111121 80-81 0.003930731771231411 82-83 0.005552938533961834 84-85 0.003587572648346129 86-87 0.004866620288191271 88-89 0.007268734148388243 90-91 0.006520023334820357 92-93 0.003681161500042115 94-95 0.0050849942754819044 96-97 0.0021213473051090153 98-99 0.003649965216143453 100-101 0.003930731771231411 102-103 7.799070974665497E-4 104-105 0.003493983796650143 106-107 6.863182457705637E-4 108-109 9.982810847571836E-4 110-111 0.0023397212923996495 112-113 0.004055516906826058 114-115 0.0026204878474876073 116-117 0.004835424004292608 118-119 0.0035251800805488048 120-121 0.006520023334820357 122-123 0.004429872313610003 124-125 0.05471828195825313 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1602755.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.31529486023271 #Duplication Level Percentage of deduplicated Percentage of total 1 89.08654041798788 65.3140597882282 2 6.683016884963447 9.799347069540179 3 1.7763314185766927 3.906967851673371 4 0.7399052304439311 2.1698548055450093 5 0.429262043255077 1.573573663677597 6 0.2626139576479943 1.1552171843625219 7 0.19762653249549353 1.014233325147873 8 0.13247185820895066 0.7769770676217724 9 0.10374299805400468 0.6845353642812511 >10 0.5446415496984066 7.0670352309728885 >50 0.027745886742684477 1.3827925068459348 >100 0.01362431136081279 1.7290246860029157 >500 9.730720075231038E-4 0.5313611945605964 >1k 0.0013269163738951417 2.0520283407661744 >5k 1.7692218318601888E-4 0.8429919207736644 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7120 0.4442350827169468 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5878 0.36674351351267037 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4358 0.2719068104607379 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4150 0.2589291563588945 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2695 0.16814797021378813 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2559 0.1596625809933521 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2417 0.15080283636613206 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2307 0.14393965390842645 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2099 0.13096199980658305 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1837 0.11461514704368415 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 3.119628389866199E-5 16-17 0.0 0.0 0.0 9.358885169598597E-5 1.2478513559464796E-4 18-19 3.119628389866199E-5 0.0 0.0 1.8717770339197196E-4 1.2478513559464796E-4 20-21 6.239256779732398E-5 0.0 0.0 4.3674797458126787E-4 1.5598141949330996E-4 22-23 6.239256779732398E-5 0.0 0.0 0.0016222067627304237 1.8717770339197196E-4 24-25 1.8717770339197196E-4 0.0 0.0 0.003587572648346129 2.495702711892959E-4 26-27 2.1837398729063394E-4 0.0 0.0 0.006676004754313666 2.495702711892959E-4 28-29 2.495702711892959E-4 0.0 0.0 0.012447317275566135 2.495702711892959E-4 30-31 3.119628389866199E-4 0.0 0.0 0.02807665550879579 2.495702711892959E-4 32-33 3.119628389866199E-4 0.0 0.0 0.049071754572595314 2.495702711892959E-4 34-35 3.119628389866199E-4 0.0 0.0 0.08301331145433956 2.495702711892959E-4 36-37 3.119628389866199E-4 0.0 0.0 0.13046285926420445 2.495702711892959E-4 38-39 3.119628389866199E-4 0.0 0.0 0.19878272100227423 2.495702711892959E-4 40-41 3.119628389866199E-4 0.0 0.0 0.2806729662362619 3.119628389866199E-4 42-43 3.431591228852819E-4 0.0 0.0 0.3459043958683642 3.119628389866199E-4 44-45 3.743554067839439E-4 0.0 0.0 0.4166263714666309 3.119628389866199E-4 46-47 4.991405423785918E-4 0.0 0.0 0.5080002870058118 3.119628389866199E-4 48-49 5.615331101759158E-4 0.0 0.0 0.6121022863756469 3.119628389866199E-4 50-51 5.615331101759158E-4 0.0 0.0 0.7186687921734762 3.119628389866199E-4 52-53 5.615331101759158E-4 0.0 0.0 0.8139734394838887 3.119628389866199E-4 54-55 5.615331101759158E-4 0.0 0.0 0.9118361820739913 3.119628389866199E-4 56-57 6.239256779732398E-4 0.0 0.0 1.0225829899142416 3.119628389866199E-4 58-59 6.239256779732398E-4 0.0 0.0 1.1566958143945894 3.119628389866199E-4 60-61 6.239256779732398E-4 0.0 0.0 1.3166391619430293 3.119628389866199E-4 62-63 6.239256779732398E-4 0.0 0.0 1.4685338682456146 3.119628389866199E-4 64-65 6.239256779732398E-4 0.0 0.0 1.6134718032387982 3.119628389866199E-4 66-67 6.239256779732398E-4 0.0 0.0 1.7812454180458024 3.119628389866199E-4 68-69 6.239256779732398E-4 0.0 0.0 1.980808046145543 3.119628389866199E-4 70-71 6.239256779732398E-4 0.0 0.0 2.20866570374137 3.119628389866199E-4 72-73 6.239256779732398E-4 0.0 0.0 2.454866776269611 4.3674797458126787E-4 74-75 6.239256779732398E-4 0.0 0.0 2.6950469660054095 5.615331101759158E-4 76-77 6.239256779732398E-4 0.0 0.0 2.936225436825965 5.615331101759158E-4 78-79 6.239256779732398E-4 3.119628389866199E-5 0.0 3.2089433506680685 5.615331101759158E-4 80-81 6.239256779732398E-4 9.358885169598597E-5 0.0 3.5212493487775736 5.615331101759158E-4 82-83 6.239256779732398E-4 1.2478513559464796E-4 0.0 3.860228169620435 6.239256779732398E-4 84-85 6.551219618719018E-4 1.2478513559464796E-4 0.0 4.238171149052725 6.239256779732398E-4 86-87 6.863182457705638E-4 1.2478513559464796E-4 0.0 4.635518217069983 6.239256779732398E-4 88-89 6.863182457705638E-4 1.2478513559464796E-4 0.0 5.02890335703211 6.239256779732398E-4 90-91 8.734959491625357E-4 1.8717770339197196E-4 0.0 5.454358276842063 6.239256779732398E-4 92-93 9.670848008585216E-4 1.8717770339197196E-4 0.0 5.912007761635434 6.239256779732398E-4 94-95 9.982810847571836E-4 1.8717770339197196E-4 0.0 6.381012693767918 6.239256779732398E-4 96-97 9.982810847571836E-4 1.8717770339197196E-4 0.0 6.8727285205786295 6.551219618719018E-4 98-99 0.0011542625042504937 1.8717770339197196E-4 0.0 7.391803488368465 6.863182457705638E-4 100-101 0.0011854587881491557 1.8717770339197196E-4 0.0 7.967218944879287 6.863182457705638E-4 102-103 0.0011854587881491557 1.8717770339197196E-4 0.0 8.56702989539886 6.863182457705638E-4 104-105 0.0012166550720478177 1.8717770339197196E-4 0.0 9.1746087206092 6.863182457705638E-4 106-107 0.0012790476398451417 1.8717770339197196E-4 0.0 9.771144061319415 6.863182457705638E-4 108-109 0.0013102439237438037 1.8717770339197196E-4 0.0 10.412352480572515 6.863182457705638E-4 110-111 0.0013102439237438037 1.8717770339197196E-4 0.0 11.139631447102023 6.863182457705638E-4 112-113 0.0013102439237438037 1.8717770339197196E-4 0.0 11.891961029602154 6.863182457705638E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9055 0.0 27.08388 1 TACATGG 8880 0.0 26.401138 2 GTATAGG 510 0.0 24.510447 1 GAGTACT 3075 0.0 23.600359 12-13 CCCTATA 550 0.0 22.719357 2 ACATGGG 9420 0.0 22.550528 3 AGTACTT 3430 0.0 21.071043 12-13 CATGGGG 3355 0.0 20.593914 4 TAAGGTG 640 0.0 19.54336 5 GTACTTT 3680 0.0 19.110214 14-15 CTCAACG 460 1.2551027E-10 18.127739 4 CATGGGA 6005 0.0 17.853893 4 GTATAGT 475 2.0008883E-10 17.54432 1 TACCGTG 170 0.0096630175 17.509102 7 GGATCGA 210 0.0016231254 17.008842 6 GTATTAG 555 1.05501385E-10 16.087938 1 ACTTTTT 4830 0.0 15.98816 16-17 GTGTAGC 935 0.0 15.915875 1 GTATAAA 1200 0.0 15.873432 1 TACTTTT 4475 0.0 15.648625 14-15 >>END_MODULE