FastQCFastQC Report
Thu 26 May 2016
SRR1364468_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364468_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602755
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA74050.4620169645391841No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA67360.42027633668277437No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59550.37154774123306433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57790.3605666493007353No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT50060.3123371943934039No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG38630.24102248940106255No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT32810.20471001494302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32410.202214312231127No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC30090.18773923650214785No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA28410.17725728511219743No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG18050.11261858487416979No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG17960.11205705176399387No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG17150.10700325377241064No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT17050.10637932809443738No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA16140.10070160442488092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT83450.027.08270312-13
AGTACTT86800.025.28374512-13
GTACTTT92050.024.87549214-15
GTACATG81100.023.319361
TACATGG78450.022.4059832
ACTTTTT109350.022.05498916-17
TACTTTT102700.021.48514214-15
ACATGGG78500.019.6591643
GTATCAA239650.017.9488261
CATGGGG28000.017.8754184
TATACTA8800.017.5749135
TATCAAC263900.016.0984862
ATAAGAC7600.015.6801893
CGATACT2300.002962114515.543844
GTATAGA5451.36788E-915.3253281
ATCAACG271500.015.2089183
TCAACGC270900.015.1546224
AACGCAG263800.015.0627466
GTATAAA10700.015.0542191
CAACGCA268950.015.0397235
Produced by FastQC (version 0.11.5)