FastQCFastQC Report
Thu 26 May 2016
SRR1364467_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364467_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975240
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15750.050.6450731
GTATCAA27050.049.2939641
ATCAACG30800.041.3208353
TCAACGC31600.040.4629334
CAACGCA32750.039.227665
GTACATG79700.038.6140371
AACGCAG34450.037.464556
ACATGGG82100.035.9289473
TACATGG85950.035.426652
TATCAAC36550.035.1457142
CATGGGG43850.031.193514
ACGCAGA41200.031.0378237
CGCAGAG42000.030.4466258
CATGGGA42450.028.4396574
TAAGGTG8100.024.9657065
ATGGGGA27700.022.1160455
GCAGAGT55650.021.8040399
ACCGTAT1653.2247766E-421.6280468
CCGTTGC3609.458745E-1121.478949
AGAGTAC44350.021.25727510-11
Produced by FastQC (version 0.11.5)