##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364467_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1975240 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.850271865697334 31.0 31.0 34.0 27.0 34.0 2 30.93496486502906 31.0 31.0 34.0 27.0 34.0 3 30.90190812255726 31.0 31.0 34.0 27.0 34.0 4 34.53103470970616 37.0 35.0 37.0 31.0 37.0 5 34.70197900002025 37.0 35.0 37.0 32.0 37.0 6 34.80869818351188 37.0 35.0 37.0 32.0 37.0 7 34.6735237236994 37.0 35.0 37.0 32.0 37.0 8 34.57324375772058 37.0 35.0 37.0 31.0 37.0 9 35.71852736882607 39.0 35.0 39.0 30.0 39.0 10-11 35.92438640367753 39.0 36.0 39.0 30.5 39.0 12-13 36.01414764788076 39.0 36.0 39.0 30.5 39.0 14-15 37.175253386930194 40.0 37.0 41.0 31.0 41.0 16-17 37.305890929709804 40.0 37.0 41.0 31.0 41.0 18-19 37.2801953180373 40.0 37.0 41.0 31.0 41.0 20-21 37.2069882647172 40.0 37.0 41.0 31.0 41.0 22-23 37.100075180737534 40.0 37.0 41.0 30.5 41.0 24-25 37.14140610761224 40.0 37.0 41.0 30.5 41.0 26-27 37.13232062939187 40.0 37.0 41.0 30.0 41.0 28-29 37.076379072922784 40.0 37.0 41.0 30.0 41.0 30-31 36.853167463194346 40.0 36.0 41.0 30.0 41.0 32-33 36.92990573297422 40.0 36.0 41.0 30.0 41.0 34-35 36.885633897652944 40.0 36.0 41.0 30.0 41.0 36-37 36.780662603025455 40.0 36.0 41.0 30.0 41.0 38-39 36.65204203033555 40.0 36.0 41.0 30.0 41.0 40-41 36.62627857880561 40.0 36.0 41.0 30.0 41.0 42-43 36.5015818330937 40.0 36.0 41.0 30.0 41.0 44-45 36.483302282254314 40.0 35.5 41.0 30.0 41.0 46-47 36.34727374901278 39.5 35.0 41.0 29.0 41.0 48-49 36.17903394017942 39.0 35.0 41.0 29.0 41.0 50-51 36.04223436139406 39.0 35.0 41.0 28.5 41.0 52-53 35.9181104574634 39.0 35.0 41.0 28.0 41.0 54-55 35.783739697454486 39.0 35.0 40.0 28.0 41.0 56-57 35.73680160385574 39.0 35.0 40.5 28.5 41.0 58-59 35.71500855592232 39.0 35.0 41.0 28.5 41.0 60-61 35.52075697130475 38.0 34.5 40.0 28.0 41.0 62-63 35.2567067799356 38.0 34.0 40.0 28.0 41.0 64-65 34.949308691602035 37.0 34.0 40.0 28.0 41.0 66-67 34.6401363884895 37.0 34.0 40.0 28.0 41.0 68-69 34.327216186387474 36.0 34.0 39.0 28.0 41.0 70-71 33.97829175188838 36.0 33.5 39.0 27.0 41.0 72-73 33.63826547660031 35.0 33.0 38.5 26.5 40.0 74-75 33.2733829813086 35.0 33.0 37.0 26.0 39.5 76-77 32.92806823474616 35.0 33.0 37.0 26.0 39.0 78-79 32.61689060569854 35.0 33.0 36.5 26.0 39.0 80-81 32.302273900893056 35.0 33.0 36.0 26.0 37.5 82-83 32.023092889977924 35.0 32.0 35.5 25.5 37.0 84-85 31.69074163139669 35.0 32.0 35.0 24.5 37.0 86-87 31.51286476580061 35.0 32.0 35.0 24.5 36.0 88-89 31.382719821388793 35.0 32.0 35.0 24.0 36.0 90-91 31.26609323423989 35.0 32.0 35.0 24.0 36.0 92-93 31.044750005062674 35.0 32.0 35.0 23.0 35.0 94-95 30.97212237500253 35.0 32.0 35.0 23.0 35.0 96-97 30.899930388205988 35.0 32.0 35.0 23.0 35.0 98-99 30.757501113788706 34.5 31.5 35.0 21.5 35.0 100-101 30.629207336829957 34.0 31.0 35.0 20.0 35.0 102-103 29.572716986290274 33.5 29.5 34.5 19.0 35.0 104-105 30.567355865616328 34.0 31.5 35.0 21.0 35.0 106-107 30.823775338693018 35.0 32.0 35.0 23.0 35.0 108-109 30.815883639456473 35.0 32.0 35.0 21.5 35.0 110-111 30.71565303456795 35.0 32.0 35.0 20.0 35.0 112-113 30.600032147992145 35.0 32.0 35.0 19.0 35.0 114-115 30.493498764707073 35.0 32.0 35.0 18.0 35.0 116-117 30.366844029080013 35.0 32.0 35.0 16.5 35.0 118-119 30.09475886474555 35.0 31.5 35.0 4.5 35.0 120-121 29.954558939673152 35.0 31.0 35.0 2.0 35.0 122-123 29.70641390413317 35.0 31.0 35.0 2.0 35.0 124-125 28.445075028857254 34.0 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5948401620347781 1101 2 0.6512544052931588 1101 3 0.8653713288776252 1101 4 0.7029196174212728 1101 5 0.6127144679542269 1101 6 0.556465329743915 1101 7 0.6874615785424041 1101 8 0.7860002479174852 1101 9 1.2846417666250787 1101 10-11 1.0472174506184615 1101 12-13 0.8782168288567718 1101 14-15 1.0891010363660882 1101 16-17 0.7736200617668487 1101 18-19 0.9539709821552194 1101 20-21 1.0181194193749903 1101 22-23 1.0214161891133742 1101 24-25 0.9776102613957889 1101 26-27 0.9493806223195307 1101 28-29 0.8958268446680719 1101 30-31 1.0833975874446509 1101 32-33 0.9894892421222323 1101 34-35 0.9514451138959643 1101 36-37 0.9179595150979338 1101 38-39 1.0561941632071665 1101 40-41 0.9583225071434924 1101 42-43 0.9560920153468118 1101 44-45 0.8042717881241259 1101 46-47 0.8576233794245596 1101 48-49 0.7952015036200706 1101 50-51 0.8170117810869897 1101 52-53 0.6117487550026652 1101 54-55 0.5974970910487585 1101 56-57 0.623894225017537 1101 58-59 0.6999542781381791 1101 60-61 0.6401640068750964 1101 62-63 0.6833592921193414 1101 64-65 0.6148045346698012 1101 66-67 0.6838265247831998 1101 68-69 0.6109644735767006 1101 70-71 0.6151051512529193 1101 72-73 0.709748315734565 1101 74-75 0.6203260691671275 1101 76-77 0.7323647106535986 1101 78-79 0.6404140872599839 1101 80-81 0.7348103203044616 1101 82-83 0.6715906330507089 1101 84-85 0.7754630258658821 1101 86-87 0.7594585882478988 1101 88-89 0.6851620565551038 1101 90-91 0.7646491683106547 1101 92-93 0.7966791854715964 1101 94-95 0.7143769796831734 1101 96-97 0.7276926166904012 1101 98-99 0.6819295540174899 1101 100-101 0.6593941177405043 1101 102-103 0.5573852723777648 1101 104-105 0.5732212548355022 1101 106-107 0.5355321204819319 1101 108-109 0.4599661915619784 1101 110-111 0.5807288421103962 1101 112-113 0.48229593082294997 1101 114-115 0.4639515870713069 1101 116-117 0.47454556931019454 1101 118-119 0.41683768461546933 1101 120-121 0.2905749717226236 1101 122-123 0.32271022777149483 1101 124-125 -0.036041207787206986 1102 1 0.34917529676890524 1102 2 0.3262036925973817 1102 3 0.43153328994830886 1102 4 0.3447318846760936 1102 5 0.32216320434978485 1102 6 0.31053281784024733 1102 7 0.3590945417360345 1102 8 0.47266043464338736 1102 9 0.8257565431177554 1102 10-11 0.5686808243325743 1102 12-13 0.5427266715059176 1102 14-15 0.6769392839979531 1102 16-17 0.46529614474178516 1102 18-19 0.4951745478351768 1102 20-21 0.5946636930426692 1102 22-23 0.6607682604116221 1102 24-25 0.5981769709079501 1102 26-27 0.590457953864437 1102 28-29 0.6169305742083182 1102 30-31 0.6074194327824145 1102 32-33 0.48131168336014696 1102 34-35 0.41073186065480627 1102 36-37 0.34802811726939353 1102 38-39 0.4754350678847814 1102 40-41 0.39957145870094735 1102 42-43 0.42769914120671615 1102 44-45 0.29612236903244593 1102 46-47 0.3714788628177317 1102 48-49 0.28395858610330293 1102 50-51 0.2926703266518871 1102 52-53 0.3469803578137771 1102 54-55 0.24616589810687373 1102 56-57 0.33577261943190706 1102 58-59 0.41197230500795 1102 60-61 0.28056087002666175 1102 62-63 0.3340923145448471 1102 64-65 0.3088646552887937 1102 66-67 0.3793855092097047 1102 68-69 0.3523314305474159 1102 70-71 0.29986553716291553 1102 72-73 0.24054819759058432 1102 74-75 0.3047468071235855 1102 76-77 0.3891632177161739 1102 78-79 0.17534282620943742 1102 80-81 0.21790477377290784 1102 82-83 0.19467257076120248 1102 84-85 0.34622550327820534 1102 86-87 0.3548505786531244 1102 88-89 0.27780382053724395 1102 90-91 0.0729323147905383 1102 92-93 0.18619266016342806 1102 94-95 0.30647657535661565 1102 96-97 0.21405260429615325 1102 98-99 0.18355382056071434 1102 100-101 0.09438753529601485 1102 102-103 0.11707718775452491 1102 104-105 0.14742140868701625 1102 106-107 0.10008340519112124 1102 108-109 0.103453994986765 1102 110-111 0.0860397252451719 1102 112-113 0.08914332523337265 1102 114-115 -0.07688930728842891 1102 116-117 -0.03294837666355832 1102 118-119 0.039084033999536416 1102 120-121 -0.027274969860911824 1102 122-123 0.09050950195986118 1102 124-125 -0.17458213176821147 1103 1 0.5776312985421228 1103 2 0.637279546460281 1103 3 0.7525694804614567 1103 4 0.7203625977369441 1103 5 0.6995218998437878 1103 6 0.5102380052884357 1103 7 0.5605760069706562 1103 8 0.6666686684738821 1103 9 1.1734122255089474 1103 10-11 0.9446276716055877 1103 12-13 0.8137931467908217 1103 14-15 0.9768905457615062 1103 16-17 0.9402266384489408 1103 18-19 0.8997083426668198 1103 20-21 0.8440601592990831 1103 22-23 0.8625264221681519 1103 24-25 0.7577966460552616 1103 26-27 0.8734052969556743 1103 28-29 0.9063962082683119 1103 30-31 1.0586884722764154 1103 32-33 1.0522226539343649 1103 34-35 0.8794653645754877 1103 36-37 0.8593889664409033 1103 38-39 0.9467237439741751 1103 40-41 0.9172471362119907 1103 42-43 0.9862725470857185 1103 44-45 0.8531558596455326 1103 46-47 0.7662390700617436 1103 48-49 0.8257809385003654 1103 50-51 0.8512467905336081 1103 52-53 0.798433562885343 1103 54-55 0.6673378639407588 1103 56-57 0.7122568843207375 1103 58-59 0.8037774271414122 1103 60-61 0.8177682745268129 1103 62-63 0.8679168826230494 1103 64-65 0.752691096130043 1103 66-67 0.6018210417672023 1103 68-69 0.6037178129871421 1103 70-71 0.7564877685896079 1103 72-73 0.7210113036313643 1103 74-75 0.7811535252813968 1103 76-77 0.7089508468087047 1103 78-79 0.5879805918681527 1103 80-81 0.6895404804467518 1103 82-83 0.6362965642301539 1103 84-85 0.7602571382108891 1103 86-87 0.800297075521204 1103 88-89 0.7352563524264148 1103 90-91 0.6829174423524833 1103 92-93 0.6234218819312609 1103 94-95 0.6295802920531166 1103 96-97 0.6143209598520301 1103 98-99 0.6215609349513151 1103 100-101 0.6031709626928077 1103 102-103 0.6111673338785479 1103 104-105 0.5413699271057908 1103 106-107 0.5803210098934457 1103 108-109 0.5008011670829369 1103 110-111 0.5463561294051544 1103 112-113 0.5590479387471703 1103 114-115 0.429476927131315 1103 116-117 0.4125445174871558 1103 118-119 0.21186313843496762 1103 120-121 0.029510717083546467 1103 122-123 0.1345277639303042 1103 124-125 -0.034327153392506915 1104 1 0.33087836779238344 1104 2 0.46166633717203354 1104 3 0.6829058823414762 1104 4 0.4866810448762209 1104 5 0.4820847147327427 1104 6 0.47032926779712625 1104 7 0.5807412725728511 1104 8 0.7516776806930139 1104 9 1.188847545283437 1104 10-11 0.8781357230093647 1104 12-13 0.8228271721710243 1104 14-15 0.8761121605705426 1104 16-17 0.7412100127037888 1104 18-19 0.7747500735407797 1104 20-21 0.7508310670597709 1104 22-23 0.8741337397477835 1104 24-25 0.9117358343513615 1104 26-27 0.8610686814465751 1104 28-29 0.8100869702745896 1104 30-31 0.8728647826670937 1104 32-33 0.7867348032635633 1104 34-35 0.6941488378296796 1104 36-37 0.696994726755257 1104 38-39 0.7861003986458499 1104 40-41 0.7836420444164389 1104 42-43 0.8143611421177681 1104 44-45 0.6794655698502652 1104 46-47 0.7278860513034147 1104 48-49 0.7096182178886465 1104 50-51 0.7098794984244989 1104 52-53 0.44129332013589107 1104 54-55 0.506962841799421 1104 56-57 0.5428088639261688 1104 58-59 0.5680280437479297 1104 60-61 0.5925802819658941 1104 62-63 0.5716081910743753 1104 64-65 0.5231078295557978 1104 66-67 0.4296391980533727 1104 68-69 0.3535451672837553 1104 70-71 0.4380403200033882 1104 72-73 0.42612878209884997 1104 74-75 0.38132674836068503 1104 76-77 0.40765594732675936 1104 78-79 0.24111794938775688 1104 80-81 0.35585757693883835 1104 82-83 0.4168499047930574 1104 84-85 0.34738120206288414 1104 86-87 0.3712552328837653 1104 88-89 0.3876423480090736 1104 90-91 0.2510823891606968 1104 92-93 0.28710351521219835 1104 94-95 0.32245343205213217 1104 96-97 0.3409286785017045 1104 98-99 0.34184138704489087 1104 100-101 0.3517714963118621 1104 102-103 0.33538084995960205 1104 104-105 0.3176122802260011 1104 106-107 0.29496493289871495 1104 108-109 0.19774563197611172 1104 110-111 0.37972647665757364 1104 112-113 0.33904617362657063 1104 114-115 0.32704786549293274 1104 116-117 0.26038730710354585 1104 118-119 0.12299658505770239 1104 120-121 -0.021654118167397485 1104 122-123 0.09076370199189299 1104 124-125 -0.09586531989823044 1105 1 0.4032252815848558 1105 2 0.49795017762377825 1105 3 0.6290607943836513 1105 4 0.4920280055517594 1105 5 0.48278441832763264 1105 6 0.4442201969672439 1105 7 0.5394828271108736 1105 8 0.6606472431698975 1105 9 1.1384240870848927 1105 10-11 0.6964158440718364 1105 12-13 0.7194135125316095 1105 14-15 0.52968392030008 1105 16-17 0.6393377117354007 1105 18-19 0.676526993532228 1105 20-21 0.8500225426779835 1105 22-23 0.9340073638602675 1105 24-25 0.9244660431893408 1105 26-27 0.8337903992418276 1105 28-29 0.8202687202628667 1105 30-31 0.9421143932772509 1105 32-33 0.8088667321888465 1105 34-35 0.832653841293812 1105 36-37 0.8103637433049755 1105 38-39 0.8030209110255839 1105 40-41 0.7274820273301685 1105 42-43 0.7967727545303163 1105 44-45 0.7432053272043859 1105 46-47 0.7849492965486888 1105 48-49 0.6998139807020394 1105 50-51 0.696087909245108 1105 52-53 0.5237195704809992 1105 54-55 0.4980410479494424 1105 56-57 0.5954275952706212 1105 58-59 0.6373708995512715 1105 60-61 0.6194672990052794 1105 62-63 0.6843757500819123 1105 64-65 0.6974172160649346 1105 66-67 0.5886477776529944 1105 68-69 0.5944385500005751 1105 70-71 0.7103335787405598 1105 72-73 0.6670231545691223 1105 74-75 0.7613560763180871 1105 76-77 0.698061069040385 1105 78-79 0.6529664962734216 1105 80-81 0.5980887937625639 1105 82-83 0.6384234564972537 1105 84-85 0.7356531446363519 1105 86-87 0.7546286251855641 1105 88-89 0.674331362266738 1105 90-91 0.6661255997125117 1105 92-93 0.6754324988395943 1105 94-95 0.7630173831478935 1105 96-97 0.6050407394288406 1105 98-99 0.7103194587968069 1105 100-101 0.45947578923720656 1105 102-103 0.39735801270111537 1105 104-105 0.4931898013624476 1105 106-107 0.44493638632560817 1105 108-109 0.42300497342542087 1105 110-111 0.42375977404723386 1105 112-113 0.4391667672702688 1105 114-115 0.4915198841211321 1105 116-117 0.5247732171818384 1105 118-119 0.2476649916067437 1105 120-121 0.22952082335901025 1105 122-123 0.2933661805428933 1105 124-125 0.16530785049042862 1106 1 0.33270046008305343 1106 2 0.40081350952001316 1106 3 0.5431930144911163 1106 4 0.38632573738910736 1106 5 0.3748123050562029 1106 6 0.3034222916264966 1106 7 0.28042878868775034 1106 8 0.4538835548030349 1106 9 0.9069924211367706 1106 10-11 0.3614579580793489 1106 12-13 0.5587306452038803 1106 14-15 0.7783787541048497 1106 16-17 0.5883344414591605 1106 18-19 0.5997094665340512 1106 20-21 0.6840951407074058 1106 22-23 0.6350508028028443 1106 24-25 0.5841847573714034 1106 26-27 0.6127986958590341 1106 28-29 0.619301097049167 1106 30-31 0.7277054089556501 1106 32-33 0.6788711486390753 1106 34-35 0.598294594614039 1106 36-37 0.5574623458139456 1106 38-39 0.7451579471668666 1106 40-41 0.6413311740088972 1106 42-43 0.649889822007367 1106 44-45 0.4372298700492081 1106 46-47 0.502985553501972 1106 48-49 0.45963248562848946 1106 50-51 0.582429090540387 1106 52-53 0.3908565540454916 1106 54-55 0.3644873828215225 1106 56-57 0.33178318260592476 1106 58-59 0.5085905758885616 1106 60-61 0.5334859532498086 1106 62-63 0.5943322165011153 1106 64-65 0.5602307051657718 1106 66-67 0.5461205165882035 1106 68-69 0.625595449652522 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-0.9193701797771254 2216 78-79 -0.8929665276656777 2216 80-81 -0.9420167118966774 2216 82-83 -1.1544553566753706 2216 84-85 -1.192027066374969 2216 86-87 -1.1866266473150695 2216 88-89 -1.594891368193018 2216 90-91 -1.2938440173286558 2216 92-93 -1.0741128697068127 2216 94-95 -1.0764861392720562 2216 96-97 -1.1062899896508753 2216 98-99 -1.0852763476871168 2216 100-101 -1.2101432552020448 2216 102-103 -1.4285062322322766 2216 104-105 -1.2684687965697314 2216 106-107 -0.9846138791079326 2216 108-109 -0.9584429470669349 2216 110-111 -1.005207094059564 2216 112-113 -1.082189225295366 2216 114-115 -0.9748062263036736 2216 116-117 -0.91361314721334 2216 118-119 -0.7479275873833835 2216 120-121 -0.7214207144861362 2216 122-123 -0.7489352451190179 2216 124-125 -0.6435791093217063 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24866.0 3 4708.0 4 3389.0 5 3038.0 6 3263.0 7 3921.0 8 4342.0 9 4729.0 10 5230.0 11 5731.0 12 5965.0 13 6250.0 14 6352.0 15 6107.0 16 6026.0 17 5703.0 18 5643.0 19 5593.0 20 5698.0 21 6240.0 22 7376.0 23 9705.0 24 13225.0 25 17903.0 26 21514.0 27 25410.0 28 30846.0 29 37262.0 30 45865.0 31 56220.0 32 71426.0 33 94037.0 34 129346.0 35 193585.0 36 349563.0 37 561449.0 38 187576.0 39 138.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41819232113992 18.94936330423062 13.061612286986882 24.57083208764258 2 15.98400596991652 20.495008953355978 38.888548564045735 24.632436512681767 3 18.28304408578198 25.26735991575707 29.499402604240498 26.950193394220452 4 12.816823469909016 16.648854061349954 37.329466031927005 33.204856436814026 5 13.917456878002845 38.002136481690556 34.42256772697586 13.657838913330734 6 32.4011067037593 36.323931076555446 17.944497743306492 13.33046447637876 7 28.887881978898765 32.02005832202669 21.84919300945708 17.242866689617465 8 26.429527924808184 34.405672293902185 20.784775328255407 18.380024453034228 9 26.97908033675185 15.700505715050909 20.066341876334974 37.25407207186227 10-11 24.70637556283186 26.28936785115569 28.39001608561171 20.614240500400747 12-13 25.315182953439695 23.749494988360684 28.003869983930755 22.93145207426886 14-15 23.16188615940053 25.34877616026882 24.957136307933066 26.53220137239758 16-17 23.23198110967496 28.259570846566227 25.870890460720002 22.637557583038806 18-19 23.148082512227973 27.41351811157581 26.834170472612378 22.604228903583834 20-21 23.873511902501416 26.73598083163833 26.707853124251553 22.6826541416087 22-23 23.826724214970206 26.48938386708296 26.472877843017077 23.211014074929754 24-25 23.546038496192327 26.87670266702751 26.391721832593134 23.185537004187033 26-27 23.30543358946213 27.095468059510452 26.241590077810766 23.357508273216652 28-29 23.421610764827484 26.836199096855395 26.52059466386242 23.2215954744547 30-31 23.514320617258164 26.75641975074214 26.373886789586614 23.35537284241308 32-33 23.382775646485985 26.815242526380274 26.27010716649854 23.531874660635204 34-35 23.923952582377108 26.6609204567604 26.155355239809335 23.259771721053152 36-37 23.83445527331197 26.461827503658448 26.075997943524897 23.62771927950468 38-39 23.46821274548047 26.663784307019906 26.390120459176092 23.47788248832353 40-41 23.627367543860093 26.3904787411509 26.60134335695456 23.38081035803445 42-43 23.3935081655477 26.36350242938777 26.44030520623596 23.802684198828565 44-45 23.47599694717164 26.49789200429826 26.375044773550254 23.651066274979843 46-47 23.77624342537929 26.600881223202204 26.019499708638428 23.60337564278008 48-49 23.504955613269008 26.508322306373376 26.239852644204664 23.746869436152952 50-51 23.434415593292076 26.507816846964573 26.37729557700983 23.680471982733525 52-53 23.563660390159434 26.3076619293298 26.388468373593106 23.74020930691766 54-55 23.525921200361 26.3623487239076 26.224657575861137 23.887072499870257 56-57 23.342157607081766 26.73063822106287 26.248471869874322 23.678732301981047 58-59 23.375797500553144 26.152600632785454 26.43947552424538 24.032126342416017 60-61 23.348450449209732 26.437197725192203 26.457602540526086 23.75674928507198 62-63 23.221592763255664 26.32684490169883 26.837534522562674 23.61402781248283 64-65 23.39604872763359 26.21649468876895 26.390413455793748 23.997043127803714 66-67 23.232654908839027 26.464631325672112 26.17366458595785 24.12904917953101 68-69 23.177011800381624 26.14056304916737 26.753658330964107 23.928766819486896 70-71 23.38160738962907 25.868081177775927 26.568383661667628 24.181927770927373 72-73 23.30121322563283 26.204833155525897 26.179086098462616 24.314867520378655 74-75 23.233721794536834 26.41662767877215 26.07978746027792 24.269863066413095 76-77 23.340735407736837 26.21425410683492 26.472567264199377 23.972443221228865 78-79 23.379843053743386 26.11749147245846 26.442467131561347 24.06019834223681 80-81 23.444369196883486 26.249156629556257 26.265663272087448 24.040810901472813 82-83 23.486310583084776 26.171153346128907 26.324882532116156 24.017653538670157 84-85 23.37436493092089 26.05317415551129 26.353683223442037 24.218777690125783 86-87 23.36282804960176 25.942286562055365 26.669147767262636 24.02573762108024 88-89 23.47258781118453 25.931354144329415 26.577553117611302 24.018504926874755 90-91 23.451448077976135 25.966087843912035 26.528134350406035 24.0543297277058 92-93 23.443105859384097 25.672614520919428 26.576437910052082 24.307841709644396 94-95 23.301418194641588 25.623474271821188 26.68889866063755 24.386208872899676 96-97 23.54092153709682 25.520316468257988 26.675199792612638 24.263562202032556 98-99 23.33456449057304 25.605505323383827 26.571858277009362 24.488071909033764 100-101 23.210802047358197 25.42101828171985 26.798033248052057 24.570146422869897 102-103 23.2698710306736 25.237107054692785 26.800951592768524 24.69207032186509 104-105 22.970996606376367 25.675338697815597 26.640970821925265 24.712693873882767 106-107 23.105156849129717 25.476671035154386 26.561814523141635 24.856357592574263 108-109 23.138343316864702 25.580969126953406 26.46955109422255 24.81113646195934 110-111 23.05898435522252 25.655536428535264 26.595311629790896 24.69016758645132 112-113 23.008800270169967 25.612325340130116 26.885987363377083 24.492887026322837 114-115 23.014753257932014 26.0572028936892 26.403129133728694 24.52491471465009 116-117 22.847378743418094 25.864442145166887 26.75949795862465 24.52868115279037 118-119 22.912823182475943 25.78569442499447 26.48761865330403 24.813863739225557 120-121 22.856706628490752 25.892659103730253 26.64142424386672 24.609210023912276 122-123 22.849831952645943 26.076710819292252 26.503197128129713 24.570260099932092 124-125 22.937499444938535 26.197556207109606 26.34538651942842 24.519557828523435 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 210.0 1 170.0 2 126.0 3 100.5 4 74.5 5 65.0 6 60.5 7 62.0 8 61.0 9 58.5 10 57.0 11 66.0 12 75.0 13 86.0 14 98.0 15 106.5 16 125.5 17 155.0 18 192.5 19 223.0 20 291.5 21 384.0 22 487.0 23 680.0 24 958.0 25 1374.5 26 2125.0 27 3354.5 28 4998.5 29 7520.0 30 10766.5 31 14916.0 32 20674.0 33 25961.0 34 30387.5 35 36697.5 36 46701.5 37 55545.0 38 60678.0 39 64575.0 40 66312.0 41 67788.0 42 68946.5 43 70089.0 44 72810.5 45 75549.5 46 76132.5 47 75443.0 48 74730.0 49 74459.0 50 74501.5 51 73367.5 52 73662.0 53 75434.0 54 77546.0 55 73535.5 56 65859.0 57 60177.0 58 55913.5 59 49858.5 60 40179.0 61 32827.5 62 27181.5 63 20748.5 64 15089.5 65 11613.5 66 8748.5 67 6868.5 68 5255.5 69 3669.5 70 2689.0 71 1903.5 72 1405.5 73 1074.0 74 845.5 75 607.0 76 428.5 77 271.0 78 165.5 79 100.0 80 75.0 81 59.5 82 41.0 83 32.5 84 22.0 85 10.0 86 7.5 87 12.0 88 10.5 89 6.5 90 6.5 91 4.0 92 4.5 93 2.5 94 2.0 95 5.0 96 4.0 97 1.5 98 1.5 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08960936392539641 2 1.5188027783965495E-4 3 0.0 4 0.002379457686154594 5 0.0015188027783965493 6 2.5313379639942487E-4 7 0.0 8 0.0012656689819971244 9 8.606549077580446E-4 10-11 0.00926469694821895 12-13 0.0019238168526356288 14-15 0.004227334399870396 16-17 0.002480711204714364 18-19 0.013162957412770094 20-21 0.01614993621028331 22-23 0.010302545513456593 24-25 0.010808813106255442 26-27 0.009720337781737916 28-29 0.007138373058463782 30-31 0.01911160162815658 32-33 0.0021516372693951113 34-35 0.0012656689819971244 36-37 7.847147688382172E-4 38-39 3.290739353192523E-4 40-41 0.001974443611915514 42-43 0.0025819647232741337 44-45 0.0011391020837974118 46-47 0.0015694295376764344 48-49 0.007923087827301998 50-51 0.004202021020230453 52-53 0.007594013891982746 54-55 0.008479982179380733 56-57 0.009897531439217512 58-59 0.00825216176262125 60-61 0.010935380004455155 62-63 0.01334015107024969 64-65 0.009112816670379295 66-67 0.013770478524128714 68-69 0.01323889755168992 70-71 0.004733601992669245 72-73 0.013011077134930438 74-75 0.013567971487009174 76-77 0.005973957595026428 78-79 0.00855592231830056 80-81 0.01404892570016808 82-83 0.017137158016241064 84-85 0.013061703894210323 86-87 0.015694295376764342 88-89 0.015517101719284745 90-91 0.011416334217614062 92-93 0.009416577226058605 94-95 0.008024341345861769 96-97 0.009720337781737916 98-99 0.0067586723638646444 100-101 0.01047973917093619 102-103 0.011922601810412912 104-105 0.010758186346975557 106-107 0.005543630141147405 108-109 0.003569186529231891 110-111 0.007087746299183896 112-113 0.00946720398533849 114-115 0.012783256718170957 116-117 0.011315080699054292 118-119 0.2213401915716571 120-121 0.02642716834409996 122-123 0.025262752880662605 124-125 0.23984427208845505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1975240.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.91770186687191 #Duplication Level Percentage of deduplicated Percentage of total 1 84.362297293147 49.7043268072205 2 8.63553811891636 10.17572120700644 3 2.777220326876044 4.90882317712498 4 1.2941695711327958 3.049979878287182 5 0.6805139645252536 2.0047159439070974 6 0.4312102573765513 1.5243550431629254 7 0.2948862800206902 1.2161815351622953 8 0.2071379559366 0.9763273866548681 9 0.1445688112318658 0.7665895907466437 >10 0.9551076254929189 11.184286499997825 >50 0.1359244534707526 5.5929897167334985 >100 0.0795019086013264 8.149274034944614 >500 0.001923433271898857 0.7464291790510942 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.062675927988498E-5 0.0 10-11 0.0 0.0 0.0 5.062675927988498E-5 0.0 12-13 0.0 0.0 0.0 5.062675927988498E-5 0.0 14-15 0.0 0.0 0.0 7.594013891982747E-5 0.0 16-17 0.0 0.0 0.0 1.0125351855976995E-4 0.0 18-19 0.0 0.0 0.0 1.0125351855976995E-4 0.0 20-21 0.0 0.0 0.0 1.5188027783965495E-4 0.0 22-23 0.0 2.531337963994249E-5 0.0 3.290739353192523E-4 0.0 24-25 0.0 5.062675927988498E-5 0.0 5.062675927988497E-4 0.0 26-27 0.0 5.062675927988498E-5 0.0 0.001139102083797412 0.0 28-29 0.0 5.062675927988498E-5 0.0 0.004227334399870396 0.0 30-31 0.0 5.062675927988498E-5 0.0 0.01627650310848302 2.531337963994249E-5 32-33 0.0 5.062675927988498E-5 0.0 0.044804681962698205 5.062675927988498E-5 34-35 0.0 5.062675927988498E-5 0.0 0.09029282517567486 5.062675927988498E-5 36-37 0.0 5.062675927988498E-5 0.0 0.15762641501792188 5.062675927988498E-5 38-39 0.0 5.062675927988498E-5 0.0 0.25419695834430245 5.062675927988498E-5 40-41 0.0 5.062675927988498E-5 0.0 0.3602853324153015 5.062675927988498E-5 42-43 0.0 5.062675927988498E-5 0.0 0.46743686843117793 5.062675927988498E-5 44-45 0.0 5.062675927988498E-5 0.0 0.6053441607095846 5.062675927988498E-5 46-47 0.0 5.062675927988498E-5 0.0 0.7712986776290476 5.062675927988498E-5 48-49 0.0 5.062675927988498E-5 0.0 0.9403161134849436 5.062675927988498E-5 50-51 0.0 5.062675927988498E-5 0.0 1.1216358518458516 5.062675927988498E-5 52-53 0.0 5.062675927988498E-5 0.0 1.300145805066726 5.062675927988498E-5 54-55 0.0 5.062675927988498E-5 0.0 1.491160567829732 5.062675927988498E-5 56-57 0.0 5.062675927988498E-5 0.0 1.7233855126465645 5.062675927988498E-5 58-59 0.0 5.062675927988498E-5 0.0 1.9719882140904397 5.062675927988498E-5 60-61 0.0 5.062675927988498E-5 0.0 2.236209270772159 5.062675927988498E-5 62-63 0.0 5.062675927988498E-5 0.0 2.4948866973127313 5.062675927988498E-5 64-65 0.0 5.062675927988498E-5 0.0 2.776042404973573 5.062675927988498E-5 66-67 0.0 5.062675927988498E-5 0.0 3.0980032806140017 5.062675927988498E-5 68-69 0.0 5.062675927988498E-5 0.0 3.4514540005265184 5.062675927988498E-5 70-71 0.0 5.062675927988498E-5 0.0 3.82482635021567 5.062675927988498E-5 72-73 0.0 5.062675927988498E-5 0.0 4.203489196249569 5.062675927988498E-5 74-75 0.0 5.062675927988498E-5 0.0 4.603744355116341 5.062675927988498E-5 76-77 0.0 5.062675927988498E-5 0.0 5.032578319596606 5.062675927988498E-5 78-79 0.0 5.062675927988498E-5 0.0 5.503078106964217 5.062675927988498E-5 80-81 0.0 5.062675927988498E-5 0.0 6.008662238512788 5.062675927988498E-5 82-83 0.0 5.062675927988498E-5 0.0 6.531484781596161 5.062675927988498E-5 84-85 0.0 5.062675927988498E-5 0.0 7.059825641441041 5.062675927988498E-5 86-87 0.0 5.062675927988498E-5 0.0 7.621023268058565 5.062675927988498E-5 88-89 0.0 5.062675927988498E-5 0.0 8.223253883072438 5.062675927988498E-5 90-91 0.0 5.062675927988498E-5 0.0 8.872491444077681 5.062675927988498E-5 92-93 0.0 5.062675927988498E-5 0.0 9.54210627569308 5.062675927988498E-5 94-95 0.0 5.062675927988498E-5 0.0 10.235794131346065 5.062675927988498E-5 96-97 0.0 5.062675927988498E-5 0.0 10.953251250480953 5.062675927988498E-5 98-99 0.0 5.062675927988498E-5 0.0 11.691642534578076 5.062675927988498E-5 100-101 0.0 5.062675927988498E-5 0.0 12.483850063789717 5.062675927988498E-5 102-103 0.0 5.062675927988498E-5 0.0 13.30577550069865 5.062675927988498E-5 104-105 0.0 5.062675927988498E-5 0.0 14.131270124136813 5.062675927988498E-5 106-107 0.0 5.062675927988498E-5 0.0 14.973446264757701 5.062675927988498E-5 108-109 0.0 5.062675927988498E-5 0.0 15.843568376501082 5.062675927988498E-5 110-111 0.0 5.062675927988498E-5 0.0 16.741914906543002 5.062675927988498E-5 112-113 0.0 5.062675927988498E-5 0.0 17.67238411534801 5.062675927988498E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1575 0.0 50.645073 1 GTATCAA 2705 0.0 49.293964 1 ATCAACG 3080 0.0 41.320835 3 TCAACGC 3160 0.0 40.462933 4 CAACGCA 3275 0.0 39.22766 5 GTACATG 7970 0.0 38.614037 1 AACGCAG 3445 0.0 37.46455 6 ACATGGG 8210 0.0 35.928947 3 TACATGG 8595 0.0 35.42665 2 TATCAAC 3655 0.0 35.145714 2 CATGGGG 4385 0.0 31.19351 4 ACGCAGA 4120 0.0 31.037823 7 CGCAGAG 4200 0.0 30.446625 8 CATGGGA 4245 0.0 28.439657 4 TAAGGTG 810 0.0 24.965706 5 ATGGGGA 2770 0.0 22.116045 5 GCAGAGT 5565 0.0 21.804039 9 ACCGTAT 165 3.2247766E-4 21.628046 8 CCGTTGC 360 9.458745E-11 21.47894 9 AGAGTAC 4435 0.0 21.257275 10-11 >>END_MODULE