FastQCFastQC Report
Thu 26 May 2016
SRR1364467_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364467_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975240
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT19960.10105101152265042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21900.059.3015331
GTATCAA34100.050.8384321
ATCAACG42100.040.024583
TCAACGC42200.039.9297334
CAACGCA44150.038.2757845
AACGCAG47050.035.912056
TATCAAC47350.035.7170682
GTACATG77600.034.700061
ACATGGG81850.032.080593
TACATGG83750.031.712282
TTACGGT1004.421527E-429.7709334
ACGCAGA56800.029.747577
CGCAGAG58900.028.5859478
CATGGGG42400.027.8049264
CATGGGA43350.025.6847254
AGAGTAC61750.022.49735310-11
GAGTACA58100.022.3529011
GCAGAGT73650.022.295539
CAGAGTA64900.021.77210410-11
AGTACAT57250.021.4273852
Produced by FastQC (version 0.11.5)