FastQCFastQC Report
Thu 26 May 2016
SRR1364466_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364466_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472397
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24500.047.8804171
ATCAACG26350.042.6634143
TCAACGC27850.040.366384
GGTATCA18450.039.6978071
CAACGCA28500.039.0283285
AACGCAG29300.037.353686
GTACATG76550.036.715931
TATCAAC32700.034.9250222
TACATGG81050.034.7863242
ACATGGG79850.033.446133
CATGGGG54650.030.4755174
ACGCAGA36600.029.578327
CGCAGAG39200.027.7682328
GAGTACA62850.022.3596251
AGTACAT59700.021.4213752
ATGGGGA34450.021.0646295
CATGGGA32050.019.6725674
GTGTAGC15550.019.5297891
GCAGAGT53750.019.4771849
CAGAGTA46800.018.49307610-11
Produced by FastQC (version 0.11.5)