##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364466_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2472397 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.87836621707598 31.0 31.0 34.0 27.0 34.0 2 30.899959836547286 31.0 31.0 34.0 27.0 34.0 3 30.836788751968232 31.0 31.0 34.0 27.0 34.0 4 34.455535255867076 37.0 35.0 37.0 30.0 37.0 5 34.6094401505907 37.0 35.0 37.0 32.0 37.0 6 34.75681413624106 37.0 35.0 37.0 32.0 37.0 7 34.61144751429483 37.0 35.0 37.0 31.0 37.0 8 34.479543940556475 37.0 35.0 37.0 31.0 37.0 9 35.64337280784599 39.0 35.0 39.0 30.0 39.0 10-11 35.848028249508474 39.0 35.0 39.0 30.5 39.0 12-13 35.903517113149704 39.0 35.0 39.0 30.0 39.0 14-15 37.068435004572486 40.0 37.0 41.0 31.0 41.0 16-17 37.211723481301746 40.0 37.0 41.0 31.0 41.0 18-19 37.18153840180197 40.0 37.0 41.0 31.0 41.0 20-21 37.132768321592366 40.0 37.0 41.0 30.5 41.0 22-23 37.02815142551945 40.0 37.0 41.0 30.0 41.0 24-25 37.078440274761704 40.0 37.0 41.0 30.0 41.0 26-27 37.08715853481459 40.0 37.0 41.0 30.0 41.0 28-29 37.03459496998257 40.0 37.0 41.0 30.0 41.0 30-31 36.81191693728799 40.0 36.0 41.0 30.0 41.0 32-33 36.89422430944545 40.0 36.0 41.0 30.0 41.0 34-35 36.840255428234215 40.0 36.0 41.0 30.0 41.0 36-37 36.71858868134851 40.0 36.0 41.0 30.0 41.0 38-39 36.57768149694406 40.0 36.0 41.0 30.0 41.0 40-41 36.547808462799466 40.0 36.0 41.0 30.0 41.0 42-43 36.40853046658769 40.0 35.0 41.0 29.5 41.0 44-45 36.38067814351821 39.5 35.0 41.0 29.5 41.0 46-47 36.23127657087434 39.0 35.0 41.0 29.0 41.0 48-49 36.055326268394595 39.0 35.0 41.0 28.5 41.0 50-51 35.913253211357244 39.0 35.0 41.0 28.5 41.0 52-53 35.78304111354285 39.0 35.0 40.5 28.0 41.0 54-55 35.656763052212085 39.0 34.5 40.0 28.0 41.0 56-57 35.610880048794755 38.5 34.5 40.0 28.0 41.0 58-59 35.59740385544878 38.5 35.0 40.0 28.0 41.0 60-61 35.40953617076869 38.0 34.0 40.0 28.0 41.0 62-63 35.149661239679546 38.0 34.0 40.0 28.0 41.0 64-65 34.846626168855565 37.0 34.0 40.0 28.0 41.0 66-67 34.54882650318699 37.0 34.0 40.0 28.0 41.0 68-69 34.250421958932975 36.0 34.0 39.0 27.0 41.0 70-71 33.909923648993264 36.0 33.0 39.0 27.0 41.0 72-73 33.57873007449855 35.0 33.0 38.5 26.0 40.0 74-75 33.22191864817827 35.0 33.0 37.0 26.0 39.5 76-77 32.877222387828496 35.0 33.0 37.0 26.0 39.0 78-79 32.56816320356318 35.0 33.0 36.5 26.0 39.0 80-81 32.26139107109417 35.0 32.0 36.0 26.0 38.0 82-83 31.983669896056337 35.0 32.0 36.0 25.0 37.0 84-85 31.650420219730087 35.0 32.0 35.0 24.5 37.0 86-87 31.47232806867182 35.0 32.0 35.0 24.0 36.0 88-89 31.345429759055683 35.0 32.0 35.0 24.0 36.0 90-91 31.23087756537482 35.0 32.0 35.0 24.0 36.0 92-93 31.02219829582385 35.0 32.0 35.0 23.0 35.5 94-95 30.95708719109431 34.5 32.0 35.0 23.5 35.0 96-97 30.894693894224915 34.0 32.0 35.0 23.0 35.0 98-99 30.751759527292744 34.0 31.5 35.0 21.5 35.0 100-101 30.632784297990977 34.0 31.0 35.0 20.0 35.0 102-103 29.56679509803644 33.5 29.5 34.5 19.0 35.0 104-105 30.571114388182806 34.0 31.5 35.0 21.5 35.0 106-107 30.845154722320082 35.0 32.0 35.0 23.0 35.0 108-109 30.844945815740758 35.0 32.0 35.0 23.0 35.0 110-111 30.74947025093462 35.0 32.0 35.0 20.0 35.0 112-113 30.64365977632233 35.0 32.0 35.0 19.5 35.0 114-115 30.553856237489367 35.0 32.0 35.0 19.0 35.0 116-117 30.43862636138128 35.0 32.0 35.0 18.0 35.0 118-119 30.186362263018438 35.0 31.5 35.0 10.0 35.0 120-121 30.067615961352487 35.0 31.5 35.0 2.0 35.0 122-123 29.84106820223451 35.0 31.0 35.0 2.0 35.0 124-125 28.628193206835313 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6166547756971994 1101 2 0.6346445052411624 1101 3 0.8005000512750229 1101 4 0.6694455591500912 1101 5 0.6365708086970159 1101 6 0.5814489545809849 1101 7 0.7122812205061777 1101 8 0.7627669226540448 1101 9 1.2197149532560587 1101 10-11 0.9714016060497457 1101 12-13 0.873477424625662 1101 14-15 1.1046137912858 1101 16-17 0.7974940160256239 1101 18-19 0.889265140546307 1101 20-21 1.008366088886504 1101 22-23 0.9694101861472291 1101 24-25 0.9798819649268182 1101 26-27 0.848732989634712 1101 28-29 0.8111062284876809 1101 30-31 0.9851969221930545 1101 32-33 0.9256174443075125 1101 34-35 0.8842609457278456 1101 36-37 0.791363778301772 1101 38-39 0.9264042584295993 1101 40-41 0.9459611149320679 1101 42-43 0.9665389436160581 1101 44-45 0.8702840388022466 1101 46-47 0.8820005817318588 1101 48-49 0.7712060325491095 1101 50-51 0.7824674469452191 1101 52-53 0.6590382332674025 1101 54-55 0.5575635581216005 1101 56-57 0.6299668250428851 1101 58-59 0.6845828280949391 1101 60-61 0.6555503757784535 1101 62-63 0.6954790815093119 1101 64-65 0.7508521731109354 1101 66-67 0.7299980826058317 1101 68-69 0.567504472126565 1101 70-71 0.6180327760847817 1101 72-73 0.7003960437409376 1101 74-75 0.6839161862556722 1101 76-77 0.7135815674117367 1101 78-79 0.672004751048398 1101 80-81 0.7534444763107047 1101 82-83 0.6753447700552471 1101 84-85 0.7789301410054534 1101 86-87 0.7728120063016171 1101 88-89 0.6675540091785521 1101 90-91 0.6140062880953288 1101 92-93 0.7422058527833428 1101 94-95 0.5622555945455616 1101 96-97 0.6138108457251192 1101 98-99 0.6112337431775217 1101 100-101 0.6599874320091672 1101 102-103 0.5875631155060219 1101 104-105 0.5944429318008773 1101 106-107 0.40949882593374554 1101 108-109 0.47257049303122045 1101 110-111 0.5728948358193193 1101 112-113 0.5064724510241589 1101 114-115 0.5078758419652729 1101 116-117 0.5419870594936533 1101 118-119 0.5805009561848244 1101 120-121 0.39857537301280743 1101 122-123 0.4481962015893224 1101 124-125 0.239996946059847 1102 1 0.47431847016854434 1102 2 0.4048017061892111 1102 3 0.5292084649620001 1102 4 0.45013486493269994 1102 5 0.3570967540738579 1102 6 0.37792031633723866 1102 7 0.4938332385246582 1102 8 0.5945835371461001 1102 9 0.8998949770471327 1102 10-11 0.6504921699172357 1102 12-13 0.616710628854058 1102 14-15 0.7541161359985011 1102 16-17 0.5701749485685284 1102 18-19 0.5394732100325257 1102 20-21 0.7233159963005633 1102 22-23 0.6933283976919995 1102 24-25 0.6223095230119995 1102 26-27 0.5886653958938268 1102 28-29 0.5656246676498995 1102 30-31 0.6598179437487133 1102 32-33 0.6236000907236559 1102 34-35 0.5123169988775871 1102 36-37 0.5206784338648731 1102 38-39 0.5629222161348437 1102 40-41 0.4938340965487882 1102 42-43 0.5535278960546748 1102 44-45 0.41312781492863593 1102 46-47 0.5337838450138506 1102 48-49 0.5243720362240865 1102 50-51 0.43498809845181086 1102 52-53 0.4200076064079781 1102 54-55 0.2945388640081461 1102 56-57 0.40680282180795757 1102 58-59 0.4807601618224595 1102 60-61 0.452254595082465 1102 62-63 0.46454107207727446 1102 64-65 0.49025553737237004 1102 66-67 0.5371035119784011 1102 68-69 0.5454896041775257 1102 70-71 0.5936684513130857 1102 72-73 0.5276494553609865 1102 74-75 0.5270038033017244 1102 76-77 0.5407357477168659 1102 78-79 0.4527659375372153 1102 80-81 0.48175574909589614 1102 82-83 0.4389704946936348 1102 84-85 0.583556979866664 1102 86-87 0.5393667978371433 1102 88-89 0.491531781076489 1102 90-91 0.5158749995668224 1102 92-93 0.5399941409579831 1102 94-95 0.5209591943816463 1102 96-97 0.46703691291819993 1102 98-99 0.5309599222529187 1102 100-101 0.33456838007111855 1102 102-103 0.34888053502604777 1102 104-105 0.35906459956408554 1102 106-107 0.3618668603249269 1102 108-109 0.4183447305404613 1102 110-111 0.3709905218340417 1102 112-113 0.3164055595577686 1102 114-115 0.2805894083487921 1102 116-117 0.2037219909075958 1102 118-119 0.3525413238143855 1102 120-121 0.2714540925069038 1102 122-123 0.3530031447480013 1102 124-125 0.2148471089307442 1103 1 0.4523872647449032 1103 2 0.5001212653289819 1103 3 0.6377631897914817 1103 4 0.5497328781246864 1103 5 0.48948727882746823 1103 6 0.3060073419547038 1103 7 0.4171719887902654 1103 8 0.5124201564241133 1103 9 0.9878712230157234 1103 10-11 0.7070944767905658 1103 12-13 0.5834185019381977 1103 14-15 0.7627925679765468 1103 16-17 0.6020417932717592 1103 18-19 0.6177670835683458 1103 20-21 0.6323762674827762 1103 22-23 0.6779110347877619 1103 24-25 0.5650750398390727 1103 26-27 0.7004134732212535 1103 28-29 0.6144892812408216 1103 30-31 0.8539701530560819 1103 32-33 0.7108958710789679 1103 34-35 0.7128450663371666 1103 36-37 0.6552204578747833 1103 38-39 0.7596602851630436 1103 40-41 0.7579646135594302 1103 42-43 0.6912895900646632 1103 44-45 0.5760934017873822 1103 46-47 0.585868371963528 1103 48-49 0.5347987385388606 1103 50-51 0.46946034110071366 1103 52-53 0.4682830138877421 1103 54-55 0.5721573616034874 1103 56-57 0.5369002125579954 1103 58-59 0.5538439000119197 1103 60-61 0.5712739514140708 1103 62-63 0.6319405148832544 1103 64-65 0.6027574008044994 1103 66-67 0.45404544048046347 1103 68-69 0.46854442440361055 1103 70-71 0.5648699075157566 1103 72-73 0.48891975703674007 1103 74-75 0.5371608294420582 1103 76-77 0.5223587261711415 1103 78-79 0.47696051767535863 1103 80-81 0.5699570670708383 1103 82-83 0.4808728600286827 1103 84-85 0.5133650032491275 1103 86-87 0.5776695755191206 1103 88-89 0.5458103950501147 1103 90-91 0.5077062137791906 1103 92-93 0.5317448382153422 1103 94-95 0.4815409648424094 1103 96-97 0.48670995845603215 1103 98-99 0.5055378409716766 1103 100-101 0.5779814604189752 1103 102-103 0.47445680836205995 1103 104-105 0.5292804670501425 1103 106-107 0.5040250025776665 1103 108-109 0.5465245244838037 1103 110-111 0.5826896562559156 1103 112-113 0.6666906090471905 1103 114-115 0.6483847053242506 1103 116-117 0.4997796708066744 1103 118-119 0.4607394238923703 1103 120-121 0.4310890725765226 1103 122-123 0.43242300409503187 1103 124-125 0.03196209326122812 1104 1 0.5098420789934082 1104 2 0.5405489554614604 1104 3 0.6706923997402932 1104 4 0.5597059531967048 1104 5 0.4675649356851821 1104 6 0.4195089815141415 1104 7 0.5145868905894062 1104 8 0.6629566688601258 1104 9 1.110880781739354 1104 10-11 0.7879754751717343 1104 12-13 0.7800697104224597 1104 14-15 0.8336354385411653 1104 16-17 0.6714601238686626 1104 18-19 0.8198618539033689 1104 20-21 0.8509605412984129 1104 22-23 0.9038791549131275 1104 24-25 0.9834634933799009 1104 26-27 0.9896109180876138 1104 28-29 0.8197217670846584 1104 30-31 1.0083619760988043 1104 32-33 0.97565859838938 1104 34-35 0.8935006396878862 1104 36-37 0.7850617061506 1104 38-39 0.9802311863220154 1104 40-41 0.9647963606804097 1104 42-43 0.8674712040865842 1104 44-45 0.7866660415241356 1104 46-47 0.7825269924308529 1104 48-49 0.8352817551670242 1104 50-51 0.806949662088229 1104 52-53 0.6193775119223446 1104 54-55 0.6102329344706234 1104 56-57 0.6235169344523328 1104 58-59 0.6800290976916656 1104 60-61 0.7747969317278418 1104 62-63 0.771325303018557 1104 64-65 0.6675895129116753 1104 66-67 0.7098677330773455 1104 68-69 0.6418798369301228 1104 70-71 0.6701002777061689 1104 72-73 0.6656014280517724 1104 74-75 0.6475485725838865 1104 76-77 0.6392400141233736 1104 78-79 0.5500348988683257 1104 80-81 0.6171926996253063 1104 82-83 0.6205741326445775 1104 84-85 0.5597697021297492 1104 86-87 0.5820406306170689 1104 88-89 0.6342696783646034 1104 90-91 0.5870705621118795 1104 92-93 0.7325243773425534 1104 94-95 0.63913490848946 1104 96-97 0.685499775916881 1104 98-99 0.7306597716910801 1104 100-101 0.7303691695952068 1104 102-103 0.7148690124872132 1104 104-105 0.7027957820781339 1104 106-107 0.6784522074051544 1104 108-109 0.5868753602676477 1104 110-111 0.7014923029239526 1104 112-113 0.6660127364216173 1104 114-115 0.6656801720114878 1104 116-117 0.6525067480617004 1104 118-119 0.5286637001949792 1104 120-121 0.4209989169565418 1104 122-123 0.5359355212793169 1104 124-125 0.33015466320156506 1105 1 0.27791841030038 1105 2 0.2975685464330482 1105 3 0.3397046283219147 1105 4 0.2329772017830436 1105 5 0.1881057261272545 1105 6 0.26876141587491276 1105 7 0.2345155218987287 1105 8 0.4265269191261609 1105 9 0.856822164520743 1105 10-11 0.4154242381968274 1105 12-13 0.5906216019862285 1105 14-15 0.21177232943776403 1105 16-17 0.30612295349961727 1105 18-19 0.2433612255529951 1105 20-21 0.5854781701658993 1105 22-23 0.618977157477417 1105 24-25 0.5578510921013091 1105 26-27 0.5538545578561411 1105 28-29 0.4739909644427627 1105 30-31 0.6530369939778353 1105 32-33 0.48533839629951814 1105 34-35 0.48117423837546625 1105 36-37 0.5267657174879403 1105 38-39 0.658256263667738 1105 40-41 0.5321412019404406 1105 42-43 0.5582073838117054 1105 44-45 0.48753251883997706 1105 46-47 0.5615871673338759 1105 48-49 0.3403401094224918 1105 50-51 0.43493725381270565 1105 52-53 0.3803056258346871 1105 54-55 0.3198627419148039 1105 56-57 0.40047217534841195 1105 58-59 0.5377509260474156 1105 60-61 0.4169436217966407 1105 62-63 0.422843579102107 1105 64-65 0.37312597147315074 1105 66-67 0.35573649379229977 1105 68-69 0.4147969165096512 1105 70-71 0.3609151728989488 1105 72-73 0.35725653315824246 1105 74-75 0.3211806184675581 1105 76-77 0.3331749403570754 1105 78-79 0.22720368347982145 1105 80-81 0.24820921050184808 1105 82-83 0.3199324389479301 1105 84-85 0.4893818828026397 1105 86-87 0.36816842810624095 1105 88-89 0.3775868146713144 1105 90-91 0.25346606158212737 1105 92-93 0.2580257312630536 1105 94-95 0.3670121033969096 1105 96-97 0.35060380682827486 1105 98-99 0.39176001153833795 1105 100-101 0.2049880934875432 1105 102-103 0.17266949848474766 1105 104-105 0.16067129714401318 1105 106-107 0.12741856272287322 1105 108-109 0.2003433816210105 1105 110-111 0.2564780929388384 1105 112-113 0.29622778071840017 1105 114-115 0.2130453434023103 1105 116-117 0.1015114853016641 1105 118-119 -9.596264146480848E-4 1105 120-121 -0.005633930249143049 1105 122-123 -0.0318798780104963 1105 124-125 -0.15706263876181126 1106 1 0.3450374141467627 1106 2 0.2600333207550598 1106 3 0.418933863128391 1106 4 0.19854240824575697 1106 5 0.1697273980520677 1106 6 0.13959935399167733 1106 7 0.18871318722859343 1106 8 0.3907699020486177 1106 9 0.8622676246469467 1106 10-11 0.30162667612594873 1106 12-13 0.49042770515654865 1106 14-15 0.6634820803687376 1106 16-17 0.4618952384214552 1106 18-19 0.46726916130396745 1106 20-21 0.6403591673845881 1106 22-23 0.6212427042713955 1106 24-25 0.5730755678882389 1106 26-27 0.6167229821355278 1106 28-29 0.47527913887961404 1106 30-31 0.5262027857522966 1106 32-33 0.5180583198910682 1106 34-35 0.4859353547919767 1106 36-37 0.4367835894396137 1106 38-39 0.48311819338852047 1106 40-41 0.4998638834095104 1106 42-43 0.4358866954433438 1106 44-45 0.22679636723939467 1106 46-47 0.3646540762517603 1106 48-49 0.3586276066050331 1106 50-51 0.413161870322611 1106 52-53 0.3262918656034728 1106 54-55 0.286346641781293 1106 56-57 0.24855978731936546 1106 58-59 0.3889628012030997 1106 60-61 0.4202996052725396 1106 62-63 0.4780752188470174 1106 64-65 0.42601454696384167 1106 66-67 0.33881309089494493 1106 68-69 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-1.1072927189635209 2216 76-77 -1.0652589484570107 2216 78-79 -1.079654728159717 2216 80-81 -1.0654733322443235 2216 82-83 -1.2944884226215692 2216 84-85 -1.4275656126349148 2216 86-87 -1.4012091423317763 2216 88-89 -1.8902033147567998 2216 90-91 -1.5199192299696627 2216 92-93 -1.3955203648173402 2216 94-95 -1.3423689483868557 2216 96-97 -1.2961392825045834 2216 98-99 -1.240071078086281 2216 100-101 -1.3752559949504501 2216 102-103 -1.7864466801610668 2216 104-105 -1.5459521910758198 2216 106-107 -1.364577937696371 2216 108-109 -1.291284437734518 2216 110-111 -1.3163938188997726 2216 112-113 -1.562313076970387 2216 114-115 -1.438312551589334 2216 116-117 -1.4025775228595947 2216 118-119 -1.1396779633920957 2216 120-121 -1.1721519880126472 2216 122-123 -1.1672076590403968 2216 124-125 -1.039640127758684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31036.0 3 6113.0 4 4266.0 5 3667.0 6 3954.0 7 4840.0 8 5414.0 9 6111.0 10 6794.0 11 7538.0 12 8048.0 13 8365.0 14 8577.0 15 8315.0 16 7848.0 17 7322.0 18 6997.0 19 6900.0 20 7062.0 21 7576.0 22 8817.0 23 11724.0 24 16267.0 25 22782.0 26 27841.0 27 32329.0 28 39017.0 29 47427.0 30 58076.0 31 70987.0 32 90231.0 33 120064.0 34 165430.0 35 244695.0 36 431727.0 37 680875.0 38 246896.0 39 469.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.26955794120159 18.074136206364006 12.37379247416341 25.282513378270995 2 15.924218922214784 19.738026237822993 39.11130345799704 25.226451381965187 3 18.842119611049522 23.56907082479068 29.47940804005182 28.10940152410798 4 12.699183124705746 15.62502527961274 36.94293845364811 34.732853142033406 5 13.628974686181044 37.87980334618877 34.789078473445464 13.702143494184718 6 33.640579649181845 35.854508508359515 17.18198408666587 13.32292775579277 7 29.349788626093876 31.91491168890421 20.88856315897615 17.846736526025765 8 26.533302377955458 35.22761101062298 19.93980689021411 18.299279721207455 9 26.58613730034501 15.827600014560812 19.67068302330943 37.91557966178475 10-11 25.22971229509639 25.953894323950816 28.045903828966352 20.770489551986444 12-13 25.543369668533987 23.218112322284465 27.865472121665626 23.373045887515925 14-15 22.58441270839632 25.03761170002457 24.888661480542932 27.489314111036165 16-17 23.336006236999932 28.229826571358945 25.606646328330317 22.827520863310806 18-19 23.23375723712487 27.262518645867573 26.700396935757087 22.803327181250474 20-21 23.636014263956003 26.737777436169203 26.71664040550736 22.909567894367434 22-23 23.583193373848772 26.370814159692962 26.67213155867943 23.373860907778834 24-25 23.336063766744132 26.636834153474627 26.62943164511647 23.397670434664775 26-27 23.19430955248139 27.0044249058408 26.464488502485494 23.33677703919231 28-29 23.200765466475854 27.04488439126323 26.612337585115874 23.14201255714504 30-31 23.376888691114303 26.650923360100325 26.57753999878638 23.39464794999899 32-33 23.17678129505453 26.782457915065876 26.477667111654636 23.563093678224963 34-35 23.68841276396263 26.76281474392758 26.20391713689756 23.344855355212232 36-37 23.732795174529166 26.48229105511874 26.268812587706464 23.51610118264563 38-39 23.25960182721686 26.74003415313255 26.514766659359307 23.48559736029128 40-41 23.405882670570083 26.49555779318049 26.645596410074813 23.45296312617461 42-43 23.27015775524629 26.397859519868305 26.545492786699253 23.786489938186154 44-45 23.284572615166887 26.55591506077445 26.55114233069802 23.608369993360647 46-47 23.550118297638438 26.918329834322634 26.04487483747852 23.48667703056041 48-49 23.448569610071633 26.54858880342107 26.47319069310643 23.529650893400866 50-51 23.30296952651108 26.688404889947464 26.53458029811554 23.47404528542592 52-53 23.531212963300042 26.639787882097394 26.361373022518315 23.46762613208425 54-55 23.35853833507032 26.412100666027694 26.43129413968035 23.798066859221635 56-57 23.213276621908328 26.86976799687723 26.417573260684463 23.49938212052998 58-59 23.29649842529049 26.475789599717174 26.516765215787387 23.71094675920495 60-61 23.259176530217232 26.510962729188247 26.732935595710455 23.496925144884067 62-63 23.14431439381911 26.566848895754024 26.76706559660592 23.52177111382094 64-65 23.173774335214663 26.540810593364828 26.490329016106777 23.795086055313732 66-67 23.09547796423543 26.71622944775126 26.392575331698946 23.79571725631436 68-69 23.033034733697534 26.384402702970554 26.88169346242762 23.700869100904292 70-71 23.17910447761194 26.30052987097035 26.677850584475994 23.842515066941715 72-73 23.1819661053424 26.48037569670003 26.34411505519294 23.993543142764626 74-75 23.1160212323054 26.607733885850067 26.41146207933232 23.864782802512213 76-77 23.2823962783955 26.50278064626515 26.452725373108972 23.76209770223038 78-79 23.253925043341926 26.460620936315078 26.598574873736048 23.68687914660695 80-81 23.291539664207857 26.63056998905773 26.527093745512353 23.550796601222054 82-83 23.35484990617809 26.632295976023983 26.432920619400598 23.57993349839733 84-85 23.377598691941664 26.36961628374057 26.49473455549843 23.758050468819334 86-87 23.308730412846458 26.22167952098638 26.787982911788106 23.681607154379062 88-89 23.30513831161988 26.456563559550865 26.52875182265757 23.709546306171685 90-91 23.30375140011577 26.54817712915691 26.55624713555224 23.591824335175072 92-93 23.251706387312908 26.12338007476796 26.634062540702907 23.990850997216224 94-95 23.186065866368146 26.16727391870205 26.747283040123126 23.899377174806673 96-97 23.364508285612235 25.91877523332674 26.84271797690861 23.873998504152414 98-99 23.2658034818626 26.081448403067665 26.570923534931882 24.08182458013785 100-101 23.108550851881493 26.04003283787301 26.8523232147946 23.999093095450892 102-103 23.13904514266714 25.628186033190975 26.952677825249445 24.280090998892447 104-105 22.8600368223557 26.029740635591153 26.80675989360633 24.30346264844682 106-107 23.139769132183826 26.03604243126943 26.524033175038188 24.300155261508554 108-109 23.181619068382243 25.87428907954179 26.589628458741032 24.35446339333493 110-111 23.068134704022878 26.122516670138555 26.737931466871068 24.071417158967492 112-113 23.14592284139571 26.057163405696198 26.83843315662791 23.958480596280186 114-115 22.950171778907823 26.303491037683756 26.60038760991281 24.145949573495603 116-117 22.973555683380134 26.15549779178994 26.84645840968425 24.024488115145676 118-119 23.01960933737821 26.21492281275791 26.54068442975807 24.224783420105815 120-121 22.96644213572959 26.39539548064313 26.668400933672174 23.9697614499551 122-123 22.96761782233496 26.414859228151943 26.613647121651635 24.003875827861457 124-125 23.126480921064235 26.422378502392885 26.49020516857035 23.960935407972535 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 199.0 1 178.5 2 156.0 3 134.5 4 112.0 5 102.5 6 94.0 7 95.0 8 92.5 9 81.0 10 83.5 11 97.5 12 93.5 13 108.0 14 137.5 15 149.5 16 188.5 17 240.5 18 291.5 19 396.5 20 544.5 21 792.5 22 1169.0 23 1701.5 24 2578.5 25 4020.0 26 6253.5 27 9495.0 28 13093.5 29 16899.0 30 21106.5 31 26280.0 32 32838.0 33 37786.5 34 40908.0 35 45644.5 36 52552.0 37 59571.5 38 64967.0 39 68264.0 40 71386.0 41 77106.5 42 81542.0 43 84977.5 44 90015.0 45 94831.0 46 96095.5 47 95797.0 48 95356.5 49 93434.5 50 92198.5 51 91339.5 52 92674.0 53 94197.0 54 96594.5 55 92682.5 56 82346.5 57 75151.0 58 69495.5 59 60205.5 60 48360.5 61 40392.5 62 34178.5 63 26882.5 64 20747.0 65 16304.5 66 12184.5 67 9906.5 68 7474.5 69 4808.0 70 3582.5 71 2624.0 72 2050.5 73 1697.5 74 1295.5 75 1030.0 76 767.5 77 476.5 78 284.0 79 171.5 80 108.0 81 65.0 82 38.5 83 26.5 84 20.0 85 16.5 86 10.0 87 8.0 88 11.0 89 12.0 90 9.0 91 5.0 92 5.5 93 5.5 94 2.0 95 1.5 96 2.5 97 3.0 98 2.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08736461013340495 2 4.8535894518558304E-4 3 0.0 4 0.0019818823595077976 5 0.001294290520494888 6 2.0223289382732628E-4 7 4.044657876546525E-5 8 8.493781540747703E-4 9 2.8312605135825676E-4 10-11 0.00924204324790881 12-13 0.001900989201976867 14-15 0.0038222016933364663 16-17 0.0022043385427178565 18-19 0.013529380597048129 20-21 0.01747292202668099 22-23 0.01023298442766271 24-25 0.010597003636551897 26-27 0.010273431006428174 28-29 0.00723993759901828 30-31 0.01807962070816297 32-33 0.0018403193338286691 34-35 0.0012133973629639577 36-37 6.673685496301768E-4 38-39 1.2133973629639576E-4 40-41 0.001354960388643086 42-43 0.0022043385427178565 44-45 7.07815128395642E-4 46-47 0.0012740672311121555 48-49 0.00754328693975927 50-51 0.004064881165929258 52-53 0.007563510229142002 54-55 0.008190432200006715 56-57 0.009383606273587938 58-59 0.007887082859265725 60-61 0.010030751533835383 62-63 0.013165361388158941 64-65 0.008412888383216773 66-67 0.012680002442973357 68-69 0.012214866787170507 70-71 0.0039233181402501295 72-73 0.011992410603960448 74-75 0.012740672311121557 76-77 0.005258055239510483 78-79 0.007887082859265725 80-81 0.013428264150134464 82-83 0.01725046584347093 84-85 0.013590050465196325 86-87 0.016198854795568835 88-89 0.015713495850383254 90-91 0.011325042054330272 92-93 0.00869601443457503 94-95 0.007866859569882992 96-97 0.008898247328402357 98-99 0.006350112866178045 100-101 0.010172314559514511 102-103 0.011284595475564806 104-105 0.010414994032107303 106-107 0.004712026426176702 108-109 0.0029930468286444288 110-111 0.006552345760005372 112-113 0.00823087877877218 114-115 0.013104691520010744 116-117 0.010475663900255502 118-119 0.21877554454240158 120-121 0.02635094606570061 122-123 0.026977868036565322 124-125 0.23521707881056317 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2472397.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.57575514349173 #Duplication Level Percentage of deduplicated Percentage of total 1 82.99912678956802 56.08728669055474 2 9.562996990421954 12.924534861254047 3 3.193678424244613 6.474456936114195 4 1.4786955651019411 3.9969587775638367 5 0.8076442468745069 2.7288584934920737 6 0.5103298747262193 2.0691555994144686 7 0.3303490195720304 1.5626509120944458 8 0.20164538494313514 1.090107132698595 9 0.15337512583364926 0.9327995953593224 >10 0.7061897136630375 8.27582517352696 >50 0.03825425616171498 1.7694557985336887 >100 0.017156669480017338 1.8857267432546212 >500 5.579394092199864E-4 0.20218328613887457 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 2.0223289382732627E-5 0.0 0.0 0.0 0.0 12-13 4.044657876546525E-5 0.0 0.0 0.0 0.0 14-15 4.044657876546525E-5 0.0 0.0 0.0 0.0 16-17 4.044657876546525E-5 0.0 0.0 0.0 0.0 18-19 8.08931575309305E-5 0.0 0.0 0.0 0.0 20-21 8.08931575309305E-5 0.0 0.0 0.0 0.0 22-23 8.08931575309305E-5 0.0 0.0 6.066986814819788E-5 0.0 24-25 8.08931575309305E-5 0.0 0.0 2.0223289382732628E-4 0.0 26-27 8.08931575309305E-5 0.0 0.0 5.258055239510483E-4 0.0 28-29 8.08931575309305E-5 0.0 0.0 0.0036199687995091406 0.0 30-31 8.08931575309305E-5 0.0 0.0 0.014682108091863887 0.0 32-33 8.08931575309305E-5 0.0 0.0 0.037514201804969026 0.0 34-35 8.08931575309305E-5 0.0 0.0 0.06641328233289395 0.0 36-37 8.08931575309305E-5 0.0 0.0 0.11668837973836726 0.0 38-39 8.08931575309305E-5 0.0 0.0 0.19179767650583623 0.0 40-41 8.08931575309305E-5 0.0 0.0 0.27406601771479255 0.0 42-43 8.08931575309305E-5 2.0223289382732627E-5 0.0 0.3593274057523933 0.0 44-45 8.08931575309305E-5 4.044657876546525E-5 0.0 0.46376047212482463 0.0 46-47 8.08931575309305E-5 4.044657876546525E-5 0.0 0.5940793489071536 0.0 48-49 8.08931575309305E-5 4.044657876546525E-5 0.0 0.7241353229275072 0.0 50-51 8.08931575309305E-5 4.044657876546525E-5 0.0 0.8624828455947811 0.0 52-53 8.08931575309305E-5 4.044657876546525E-5 0.0 0.9999000969504493 0.0 54-55 8.08931575309305E-5 4.044657876546525E-5 0.0 1.1568328225604545 0.0 56-57 8.08931575309305E-5 4.044657876546525E-5 0.0 1.3405209600238148 0.0 58-59 8.08931575309305E-5 4.044657876546525E-5 0.0 1.543299882664475 0.0 60-61 8.08931575309305E-5 4.044657876546525E-5 0.0 1.754633256714031 0.0 62-63 8.08931575309305E-5 4.044657876546525E-5 0.0 1.9635802826164244 0.0 64-65 8.08931575309305E-5 4.044657876546525E-5 0.0 2.191254074487228 0.0 66-67 8.08931575309305E-5 4.044657876546525E-5 0.0 2.454439962514111 0.0 68-69 1.2133973629639576E-4 4.044657876546525E-5 0.0 2.748729269611636 0.0 70-71 1.2133973629639576E-4 4.044657876546525E-5 0.0 3.0552334434963315 0.0 72-73 1.2133973629639576E-4 4.044657876546525E-5 0.0 3.365923838283253 0.0 74-75 1.2133973629639576E-4 4.044657876546525E-5 0.0 3.696594842980314 0.0 76-77 1.2133973629639576E-4 4.044657876546525E-5 0.0 4.066842824999384 0.0 78-79 1.2133973629639576E-4 4.044657876546525E-5 0.0 4.463542869531066 0.0 80-81 1.2133973629639576E-4 4.044657876546525E-5 0.0 4.8862500642089435 0.0 82-83 1.2133973629639576E-4 4.044657876546525E-5 0.0 5.309624627436451 0.0 84-85 1.2133973629639576E-4 4.044657876546525E-5 0.0 5.752777567680271 0.0 86-87 1.2133973629639576E-4 4.044657876546525E-5 0.0 6.222908375960657 0.0 88-89 1.2133973629639576E-4 4.044657876546525E-5 0.0 6.736296800230708 0.0 90-91 1.2133973629639576E-4 4.044657876546525E-5 0.0 7.277896713189669 0.0 92-93 1.2133973629639576E-4 4.044657876546525E-5 0.0 7.837556023567412 0.0 94-95 1.2133973629639576E-4 4.044657876546525E-5 0.0 8.408216803369363 0.0 96-97 1.2133973629639576E-4 4.044657876546525E-5 0.0 9.01534017392838 0.0 98-99 1.2133973629639576E-4 4.044657876546525E-5 0.0 9.647520200032599 0.0 100-101 1.2133973629639576E-4 4.044657876546525E-5 0.0 10.324676821724019 0.0 102-103 1.2133973629639576E-4 4.044657876546525E-5 0.0 11.020802888856442 0.0 104-105 1.2133973629639576E-4 4.044657876546525E-5 0.0 11.723501525038252 0.0 106-107 1.2133973629639576E-4 4.044657876546525E-5 0.0 12.440882269311928 0.0 108-109 1.61786315061861E-4 4.044657876546525E-5 0.0 13.197395078541188 0.0 110-111 1.61786315061861E-4 4.044657876546525E-5 0.0 13.985112423288008 0.0 112-113 1.61786315061861E-4 4.044657876546525E-5 0.0 14.790889165453606 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2450 0.0 47.880417 1 ATCAACG 2635 0.0 42.663414 3 TCAACGC 2785 0.0 40.36638 4 GGTATCA 1845 0.0 39.697807 1 CAACGCA 2850 0.0 39.028328 5 AACGCAG 2930 0.0 37.35368 6 GTACATG 7655 0.0 36.71593 1 TATCAAC 3270 0.0 34.925022 2 TACATGG 8105 0.0 34.786324 2 ACATGGG 7985 0.0 33.44613 3 CATGGGG 5465 0.0 30.475517 4 ACGCAGA 3660 0.0 29.57832 7 CGCAGAG 3920 0.0 27.768232 8 GAGTACA 6285 0.0 22.359625 1 AGTACAT 5970 0.0 21.421375 2 ATGGGGA 3445 0.0 21.064629 5 CATGGGA 3205 0.0 19.672567 4 GTGTAGC 1555 0.0 19.529789 1 GCAGAGT 5375 0.0 19.477184 9 CAGAGTA 4680 0.0 18.493076 10-11 >>END_MODULE