FastQCFastQC Report
Thu 26 May 2016
SRR1364466_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364466_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472397
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25250.058.7632061
GTATCAA41200.052.6467671
ATCAACG51200.041.8715133
TCAACGC52200.041.0693744
CAACGCA53550.040.0024345
AACGCAG55300.038.8425646
GTACATG76150.038.343671
TATCAAC55900.038.2452552
TACATGG79500.035.656172
ACATGGG81000.034.0394133
ACGCAGA65400.032.752967
CGCAGAG68000.031.3231058
CATGGGG56200.030.1991464
GACCGTA800.005362848329.7506087
GCAGAGT85350.024.3980549
CATGGGA33200.024.214854
AGTACAT57950.023.224692
GAGTACT48100.022.57399712-13
GTGGTAT15950.022.4159981
GAGTACA64500.022.0803381
Produced by FastQC (version 0.11.5)