FastQCFastQC Report
Thu 26 May 2016
SRR1364465_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364465_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2290387
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32850.052.552351
GGTATCA19750.050.6374321
TCAACGC38250.041.8292854
CAACGCA38450.041.616255
ATCAACG38550.041.5028573
AACGCAG40850.038.8791476
GTACATG111000.038.291711
TATCAAC43550.038.103612
TACATGG115200.036.243882
ACATGGG118900.033.615343
ACGCAGA51750.030.4602187
CGCAGAG51950.029.8849478
CATGGGA75200.029.4228464
GAGTACA80800.026.0071541
AGTACAT81300.024.8735892
CATGGGG54100.024.8468174
GTATAGG7100.022.6378361
CGTATAG1852.6818338E-522.5245341
GCAGAGT66050.022.5146249
ATGGGAT36050.022.1109055
Produced by FastQC (version 0.11.5)