##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364465_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2290387 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.902614710963693 31.0 31.0 34.0 27.0 34.0 2 30.962335186149765 31.0 31.0 34.0 27.0 34.0 3 30.900756946315187 31.0 31.0 34.0 27.0 34.0 4 34.5003416453202 37.0 35.0 37.0 31.0 37.0 5 34.69684380849175 37.0 35.0 37.0 32.0 37.0 6 34.8074622323651 37.0 35.0 37.0 32.0 37.0 7 34.673517619511465 37.0 35.0 37.0 32.0 37.0 8 34.54546677046281 37.0 35.0 37.0 31.0 37.0 9 35.63890338183023 39.0 35.0 39.0 30.0 39.0 10-11 35.899261347536466 39.0 36.0 39.0 30.5 39.0 12-13 35.967245055093315 39.0 36.0 39.0 30.5 39.0 14-15 37.14792783926909 40.0 37.0 41.0 31.0 41.0 16-17 37.31349265429816 40.0 37.0 41.0 31.0 41.0 18-19 37.29449477315406 40.0 37.0 41.0 31.0 41.0 20-21 37.24138933726047 40.0 37.0 41.0 31.0 41.0 22-23 37.1512788013554 40.0 37.0 41.0 30.0 41.0 24-25 37.22075636126122 40.0 37.0 41.0 30.5 41.0 26-27 37.24144893417575 40.0 37.0 41.0 30.5 41.0 28-29 37.1897092063481 40.0 37.0 41.0 30.0 41.0 30-31 36.95533789704535 40.0 36.0 41.0 30.0 41.0 32-33 37.04085030171757 40.0 37.0 41.0 30.0 41.0 34-35 36.989429297319624 40.0 36.5 41.0 30.0 41.0 36-37 36.88094108113607 40.0 36.0 41.0 30.0 41.0 38-39 36.7392248558868 40.0 36.0 41.0 30.0 41.0 40-41 36.708077062959234 40.0 36.0 41.0 30.0 41.0 42-43 36.58198003219543 40.0 36.0 41.0 30.0 41.0 44-45 36.57341837864082 40.0 35.5 41.0 30.0 41.0 46-47 36.42091205547359 40.0 35.0 41.0 30.0 41.0 48-49 36.25381365681869 39.0 35.0 41.0 29.0 41.0 50-51 36.12310932606586 39.0 35.0 41.0 28.5 41.0 52-53 35.99245062079028 39.0 35.0 41.0 29.0 41.0 54-55 35.86846851645595 39.0 35.0 40.5 29.0 41.0 56-57 35.81876752705984 39.0 35.0 40.5 28.5 41.0 58-59 35.786507258380354 39.0 35.0 41.0 29.0 41.0 60-61 35.598378570957664 38.0 35.0 40.5 29.0 41.0 62-63 35.33190264352706 38.0 34.0 40.0 28.0 41.0 64-65 35.02367787627156 37.0 34.0 40.0 28.0 41.0 66-67 34.7098734842627 37.0 34.0 40.0 28.0 41.0 68-69 34.40459276096136 36.0 34.0 39.0 28.0 41.0 70-71 34.05724578422773 36.0 34.0 39.0 28.0 41.0 72-73 33.729997812596736 35.0 33.0 38.5 27.0 40.0 74-75 33.37104821150312 35.0 33.0 37.0 26.5 39.5 76-77 33.02584606880846 35.0 33.0 37.0 26.0 39.0 78-79 32.719722256544415 35.0 33.0 36.5 26.0 39.0 80-81 32.4073746052523 35.0 33.0 36.0 26.0 38.0 82-83 32.13354860990741 35.0 32.5 36.0 26.0 37.0 84-85 31.80016193769874 35.0 32.0 35.0 25.0 37.0 86-87 31.616833749056383 35.0 32.0 35.0 25.0 36.0 88-89 31.491844173058965 35.0 32.0 35.0 25.0 36.0 90-91 31.37518572188892 35.0 32.0 35.0 25.0 36.0 92-93 31.166394805768633 35.0 32.0 35.0 24.0 35.5 94-95 31.091521432840825 35.0 32.0 35.0 24.0 35.0 96-97 31.024066893498784 35.0 32.0 35.0 24.0 35.0 98-99 30.878346104828573 35.0 32.0 35.0 23.0 35.0 100-101 30.74974338397834 34.5 31.5 35.0 21.5 35.0 102-103 29.70829471176705 33.5 29.5 34.5 19.5 35.0 104-105 30.70104833811928 34.0 31.5 35.0 23.0 35.0 106-107 30.963563799480177 35.0 32.0 35.0 23.0 35.0 108-109 30.95632484815885 35.0 32.0 35.0 23.0 35.0 110-111 30.85564841225522 35.0 32.0 35.0 22.5 35.0 112-113 30.74359005705149 35.0 32.0 35.0 20.0 35.0 114-115 30.644078489792335 35.0 32.0 35.0 19.0 35.0 116-117 30.5196017092308 35.0 32.0 35.0 18.5 35.0 118-119 30.25684043788233 35.0 32.0 35.0 12.5 35.0 120-121 30.127017835850445 35.0 32.0 35.0 4.5 35.0 122-123 29.88082166900179 35.0 31.0 35.0 2.0 35.0 124-125 28.661168833040005 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5817449099157379 1101 2 0.6489074727461244 1101 3 0.8153397962261728 1101 4 0.6913313938859034 1101 5 0.5621708190739128 1101 6 0.5425776909618492 1101 7 0.6766604097686795 1101 8 0.7741626528370844 1101 9 1.2486102970249249 1101 10-11 0.9064822223829907 1101 12-13 0.7651988432371795 1101 14-15 1.0351575758607225 1101 16-17 0.8712568777152967 1101 18-19 0.8795699587932191 1101 20-21 0.9013185797476098 1101 22-23 0.936226567900242 1101 24-25 0.9419289882421467 1101 26-27 0.8862073199133746 1101 28-29 0.8498095164285004 1101 30-31 0.965171228249389 1101 32-33 0.9366619030733219 1101 34-35 0.8847170758465595 1101 36-37 0.7521204691376795 1101 38-39 0.9516978013953192 1101 40-41 0.9150147638589132 1101 42-43 0.9044954848647819 1101 44-45 0.6777313064510437 1101 46-47 0.730292654909654 1101 48-49 0.7613523481670228 1101 50-51 0.7238710363554688 1101 52-53 0.5944718815567285 1101 54-55 0.5259880331345173 1101 56-57 0.5828696726123397 1101 58-59 0.6389907586345629 1101 60-61 0.620155605221882 1101 62-63 0.5806628885352367 1101 64-65 0.5700710523614987 1101 66-67 0.542453339867194 1101 68-69 0.4654622284886969 1101 70-71 0.5200228972618675 1101 72-73 0.5800912822912565 1101 74-75 0.5786737176252217 1101 76-77 0.6158575877595069 1101 78-79 0.5521758080780899 1101 80-81 0.5883669735530574 1101 82-83 0.596287040423249 1101 84-85 0.6335830049274485 1101 86-87 0.6326742023848126 1101 88-89 0.5479234688483814 1101 90-91 0.5192405917741567 1101 92-93 0.628770491261438 1101 94-95 0.5479797929639609 1101 96-97 0.5771479647050803 1101 98-99 0.5793976012739783 1101 100-101 0.5266298088413564 1101 102-103 0.5172997090023266 1101 104-105 0.5358219918869302 1101 106-107 0.4600019603865917 1101 108-109 0.4335944775084073 1101 110-111 0.46421053613478946 1101 112-113 0.40978856729956803 1101 114-115 0.4608236233382641 1101 116-117 0.41996067358698497 1101 118-119 0.37699459314833206 1101 120-121 0.3553299831296748 1101 122-123 0.38493000789007326 1101 124-125 0.0989509104658346 1102 1 0.29046726352685326 1102 2 0.26985099132522805 1102 3 0.39588341022808393 1102 4 0.3210515394252269 1102 5 0.2679369458955847 1102 6 0.18419943931947813 1102 7 0.23467132270486246 1102 8 0.4032602976753026 1102 9 0.7256305924279403 1102 10-11 0.43356593597570736 1102 12-13 0.41090012127170183 1102 14-15 0.5071090234121982 1102 16-17 0.3258804579264378 1102 18-19 0.3307064926128973 1102 20-21 0.5398427169609832 1102 22-23 0.5992859633839274 1102 24-25 0.5177972385004637 1102 26-27 0.4823429569336639 1102 28-29 0.49009937576363427 1102 30-31 0.5326364171222195 1102 32-33 0.42872948684262013 1102 34-35 0.26206554304562246 1102 36-37 0.3462892440818024 1102 38-39 0.44336906220031125 1102 40-41 0.35516134006316946 1102 42-43 0.40412005774487625 1102 44-45 0.3279895074184367 1102 46-47 0.28046618349549846 1102 48-49 0.2139434002965217 1102 50-51 0.24464625263676254 1102 52-53 0.2261323621537059 1102 54-55 0.15675027165373479 1102 56-57 0.21535174616514752 1102 58-59 0.31978044098327985 1102 60-61 0.26498474934950167 1102 62-63 0.37881415080713055 1102 64-65 0.34938622340538217 1102 66-67 0.28280585881236675 1102 68-69 0.2819285354986292 1102 70-71 0.35128997752043745 1102 72-73 0.34608653926034805 1102 74-75 0.3618007746907921 1102 76-77 0.3728496677225479 1102 78-79 0.27321963601807653 1102 80-81 0.3506727876332789 1102 82-83 0.3077118922186628 1102 84-85 0.4255088878792641 1102 86-87 0.3819602300084739 1102 88-89 0.4062340998628571 1102 90-91 0.34858539396310917 1102 92-93 0.390068976717604 1102 94-95 0.4164966710819904 1102 96-97 0.4026619396503577 1102 98-99 0.26866913490702515 1102 100-101 0.3379570877472666 1102 102-103 0.2644333715747429 1102 104-105 0.3023520242415181 1102 106-107 0.22185271975274645 1102 108-109 0.28823069761117637 1102 110-111 0.23020044116154637 1102 112-113 0.21732909104343534 1102 114-115 0.12001845107227282 1102 116-117 0.1204229790194482 1102 118-119 0.23988332217185615 1102 120-121 0.08728651456098291 1102 122-123 0.20501083059623326 1102 124-125 -0.03322977892576873 1103 1 0.4856775473932373 1103 2 0.5699116499985131 1103 3 0.7042868018609845 1103 4 0.6438928687973515 1103 5 0.5592635193213127 1103 6 0.512229399656178 1103 7 0.5687738560729585 1103 8 0.6242555935313945 1103 9 1.115452210478729 1103 10-11 0.850634874981651 1103 12-13 0.7724840698300213 1103 14-15 0.9347443810576195 1103 16-17 0.7929239292305468 1103 18-19 0.7823889675091138 1103 20-21 0.8189458179854796 1103 22-23 0.8152110025633945 1103 24-25 0.7063503424074753 1103 26-27 0.7880760816823766 1103 28-29 0.7720123992543648 1103 30-31 0.9582062319209541 1103 32-33 0.8191331040479284 1103 34-35 0.7390086811973546 1103 36-37 0.7137669934876243 1103 38-39 0.8034133533697627 1103 40-41 0.7869168400669437 1103 42-43 0.8929324302142732 1103 44-45 0.6550614835491118 1103 46-47 0.6307906976184086 1103 48-49 0.6192910495610491 1103 50-51 0.6033343619069385 1103 52-53 0.5585711360568553 1103 54-55 0.4913545220078106 1103 56-57 0.5471532919973754 1103 58-59 0.6264619104897022 1103 60-61 0.6098152436091908 1103 62-63 0.6615745609689085 1103 64-65 0.6651496830788233 1103 66-67 0.4698849653407535 1103 68-69 0.5193169472425936 1103 70-71 0.642740094213643 1103 72-73 0.5518892729333871 1103 74-75 0.6452466567324393 1103 76-77 0.6085586267934531 1103 78-79 0.5105164497193897 1103 80-81 0.5948816445819105 1103 82-83 0.46191684775192954 1103 84-85 0.6071552572206436 1103 86-87 0.6682295856248643 1103 88-89 0.6361777374963467 1103 90-91 0.5312496797326105 1103 92-93 0.4691083549350026 1103 94-95 0.5025287938216927 1103 96-97 0.5832613345165285 1103 98-99 0.6591064668977289 1103 100-101 0.645771681387707 1103 102-103 0.6432057603576276 1103 104-105 0.5214799680335673 1103 106-107 0.4570508346305715 1103 108-109 0.43942586346975787 1103 110-111 0.5685427484952861 1103 112-113 0.5149459865841344 1103 114-115 0.4828482168318189 1103 116-117 0.4366393530613344 1103 118-119 0.34807257835011285 1103 120-121 0.20969893970740472 1103 122-123 0.2870417440741271 1103 124-125 0.09095001811308734 1104 1 0.6055355378043075 1104 2 0.6429560991457954 1104 3 0.7929515760394672 1104 4 0.5841517845329776 1104 5 0.5971327430362834 1104 6 0.606774752478735 1104 7 0.6399270332881741 1104 8 0.7373208162636331 1104 9 1.2910181933684939 1104 10-11 0.9713679587400676 1104 12-13 0.8945623283516895 1104 14-15 1.0790496457262293 1104 16-17 0.9137336368678817 1104 18-19 0.9600085919791397 1104 20-21 0.9726934554158788 1104 22-23 0.9686047106483073 1104 24-25 1.0354766599125043 1104 26-27 0.9934153065406264 1104 28-29 0.9382777027457365 1104 30-31 1.0196652722007826 1104 32-33 1.037095808895181 1104 34-35 0.9925242577373155 1104 36-37 0.8821874956051232 1104 38-39 1.0495620912650168 1104 40-41 0.9050416713119702 1104 42-43 0.9537936826133375 1104 44-45 0.8158414903114775 1104 46-47 0.9366878172903128 1104 48-49 0.8606410650074494 1104 50-51 0.9304374441328704 1104 52-53 0.8212700236727386 1104 54-55 0.7649108916413994 1104 56-57 0.7618789420232801 1104 58-59 0.8212319050652184 1104 60-61 0.9147831467176886 1104 62-63 0.9455329262288643 1104 64-65 0.823708205478539 1104 66-67 0.8829199033970383 1104 68-69 0.8082625540185759 1104 70-71 0.9470609512334818 1104 72-73 0.9090228344355964 1104 74-75 0.9665013909935283 1104 76-77 0.945731059711072 1104 78-79 0.7560120602657534 1104 80-81 0.8746263046272986 1104 82-83 0.8518515106920006 1104 84-85 0.8284553576830582 1104 86-87 0.8069102927051475 1104 88-89 0.9188681599979844 1104 90-91 0.8420714039401602 1104 92-93 0.8447848951357848 1104 94-95 0.8710215413107427 1104 96-97 0.9054027165194007 1104 98-99 0.9411315995300136 1104 100-101 0.8726744469016126 1104 102-103 0.8585857539844675 1104 104-105 0.815013885654384 1104 106-107 0.7267079002035857 1104 108-109 0.6510081100818894 1104 110-111 0.7124582043830401 1104 112-113 0.7344618452945681 1104 114-115 0.6901547293750241 1104 116-117 0.7073168541012294 1104 118-119 0.7984203048101648 1104 120-121 0.7004517753990491 1104 122-123 0.8151164900598893 1104 124-125 0.7437313432926658 1105 1 0.29788095187484487 1105 2 0.2937882662628617 1105 3 0.5097469495993359 1105 4 0.2471485944517653 1105 5 0.23310182048710715 1105 6 0.3088668622301114 1105 7 0.26832029357120035 1105 8 0.48315372351386543 1105 9 0.7853393696868096 1105 10-11 0.4232180056151691 1105 12-13 0.37208203484419045 1105 14-15 0.13213060199258564 1105 16-17 0.29864757761574623 1105 18-19 0.32181545011741974 1105 20-21 0.6429735153702367 1105 22-23 0.6865778613344204 1105 24-25 0.6784466105059366 1105 26-27 0.6373838111927341 1105 28-29 0.597531029948243 1105 30-31 0.7616305367143639 1105 32-33 0.6608601976917754 1105 34-35 0.5748813244358217 1105 36-37 0.6279882231524496 1105 38-39 0.6358797642863365 1105 40-41 0.5124607375714092 1105 42-43 0.541082199393621 1105 44-45 0.42418205811875254 1105 46-47 0.5711408731821592 1105 48-49 0.5383610722729131 1105 50-51 0.38981039044211485 1105 52-53 0.25443295951999545 1105 54-55 0.35851351307151447 1105 56-57 0.43292496829479177 1105 58-59 0.4705029096511879 1105 60-61 0.44900608197855973 1105 62-63 0.39371609595820445 1105 64-65 0.420549267065482 1105 66-67 0.34604991940823027 1105 68-69 0.461871471943887 1105 70-71 0.4568441172442803 1105 72-73 0.3732134398216189 1105 74-75 0.37508841956982053 1105 76-77 0.3684230777860549 1105 78-79 0.23552145566634408 1105 80-81 0.24094806447089212 1105 82-83 0.3033399828497636 1105 84-85 0.43508038977336483 1105 86-87 0.41568430323291494 1105 88-89 0.35301776129146134 1105 90-91 0.34684696123898107 1105 92-93 0.3458047235536057 1105 94-95 0.329577748882695 1105 96-97 0.3081016639570784 1105 98-99 0.3006753306736023 1105 100-101 0.1698302259829454 1105 102-103 0.1971808560906645 1105 104-105 0.19437343090512726 1105 106-107 0.19315814644982154 1105 108-109 0.22645851865685174 1105 110-111 0.19948468899363192 1105 112-113 0.20778290505084485 1105 114-115 0.12910169865484988 1105 116-117 0.09995714151603607 1105 118-119 -0.02951902500819159 1105 120-121 -0.04486992151534608 1105 122-123 0.07298818171826582 1105 124-125 -0.07512901968008023 1106 1 0.40808502119636714 1106 2 0.4173692174947732 1106 3 0.494903147084667 1106 4 0.3441608920413657 1106 5 0.3771245197520088 1106 6 0.24771052686010364 1106 7 0.17484318673643173 1106 8 0.4413658763245536 1106 9 0.9154843209531904 1106 10-11 0.3026402688197791 1106 12-13 0.5105559530958175 1106 14-15 0.706541101910716 1106 16-17 0.5259807156124552 1106 18-19 0.5532817243872756 1106 20-21 0.6639692581808418 1106 22-23 0.567656291862086 1106 24-25 0.501441724217706 1106 26-27 0.6221273456940679 1106 28-29 0.6018159255436828 1106 30-31 0.5741491726901984 1106 32-33 0.6131545125342015 1106 34-35 0.5884253484277622 1106 36-37 0.5210164291866235 1106 38-39 0.5886900694423645 1106 40-41 0.5330372678758764 1106 42-43 0.5433140352419059 1106 44-45 0.4120513520630311 1106 46-47 0.5139915526950887 1106 48-49 0.5351553828064439 1106 50-51 0.464449434211879 1106 52-53 0.3594635095936809 1106 54-55 0.2333184924318985 1106 56-57 0.34727695340324516 1106 58-59 0.5076614080870598 1106 60-61 0.43544693376114907 1106 62-63 0.5916903977015835 1106 64-65 0.5758911779747464 1106 66-67 0.4947324744112862 1106 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0.7773435387876475 2204 58-59 0.7498174930784955 2204 60-61 0.7613040045112882 2204 62-63 0.6750105399532274 2204 64-65 0.6980164248753837 2204 66-67 0.6804268730800374 2204 68-69 0.7265288801555627 2204 70-71 0.6583179383681852 2204 72-73 0.6265274823253222 2204 74-75 0.6577320504459365 2204 76-77 0.5733686331349119 2204 78-79 0.5548128941988182 2204 80-81 0.6179431163805518 2204 82-83 0.5715231337689417 2204 84-85 0.6987303696984277 2204 86-87 0.7044581997729793 2204 88-89 0.6629997031662214 2204 90-91 0.5594815257731085 2204 92-93 0.6954746054413867 2204 94-95 0.6810723625733921 2204 96-97 0.7507833328350308 2204 98-99 0.7136361642253313 2204 100-101 0.6846155024224956 2204 102-103 0.7833508541036736 2204 104-105 0.8091706740213986 2204 106-107 0.755896944093319 2204 108-109 0.7616788061627844 2204 110-111 0.7266170314911058 2204 112-113 0.7743936775203295 2204 114-115 0.8043884888230863 2204 116-117 0.89220566143198 2204 118-119 0.9059367376176795 2204 120-121 0.890954627245879 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-1.0551547138696478 2216 82-83 -1.2315324524072864 2216 84-85 -1.2576635446139228 2216 86-87 -1.3089206722112188 2216 88-89 -1.7610186219057589 2216 90-91 -1.5097759612930304 2216 92-93 -1.3085352371849552 2216 94-95 -1.3479804621370555 2216 96-97 -1.292548175713744 2216 98-99 -1.2837209373939942 2216 100-101 -1.4349051086125328 2216 102-103 -1.8209343503811866 2216 104-105 -1.515991099669197 2216 106-107 -1.2706424236094875 2216 108-109 -1.2726693814151062 2216 110-111 -1.2353449918150687 2216 112-113 -1.5110448406300492 2216 114-115 -1.3219572773341959 2216 116-117 -1.2525885645261745 2216 118-119 -1.1068072961775783 2216 120-121 -0.9944948769794628 2216 122-123 -0.9952818699084283 2216 124-125 -0.885036769273146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25456.0 3 5385.0 4 3473.0 5 3186.0 6 3773.0 7 4624.0 8 4952.0 9 5410.0 10 5957.0 11 6778.0 12 7215.0 13 7718.0 14 7892.0 15 7596.0 16 7182.0 17 6705.0 18 6283.0 19 6209.0 20 6385.0 21 6822.0 22 7963.0 23 10557.0 24 15497.0 25 21756.0 26 25759.0 27 28612.0 28 34656.0 29 42530.0 30 51486.0 31 63186.0 32 81415.0 33 107441.0 34 149924.0 35 223695.0 36 403620.0 37 644663.0 38 238150.0 39 476.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.88329948841516 18.268521079768693 12.162408412242902 24.685771019573245 2 15.90897381824977 20.37706505575072 39.09064633112321 24.623314794876293 3 18.596813551596302 24.991540730889582 29.515928967462703 26.895716750051413 4 12.638419159543227 16.053216878295927 36.99626166755301 34.31210229460785 5 13.558550927631957 38.583176006440944 34.29762638690714 13.560646679019959 6 32.70640679712537 36.2339873732743 17.568656729451007 13.490949100149322 7 29.202295683913405 32.41887280959315 21.113742886021345 17.265088620472106 8 26.51393991787335 35.19377915747054 20.306283059686994 17.985997864969118 9 27.193482642151334 15.803213096888896 19.560543770979088 37.442760489980685 10-11 25.01824075703399 26.517890787751817 27.898380971801167 20.56548748341303 12-13 25.429001868931998 23.919491622552144 27.812397736498706 22.83910877201715 14-15 22.81509396396007 25.29899012921054 24.86688384411951 27.01903206270988 16-17 23.18187583516731 28.725039945038596 25.494223660343064 22.59886055945103 18-19 23.213065017899755 27.63463240172821 26.721665348319767 22.43063723205227 20-21 23.879852386406657 26.895987961745533 26.548744057400047 22.675415594447763 22-23 23.74067593902131 26.81207609717502 26.391104268360046 23.056143695443627 24-25 23.4724627720554 26.933258346554627 26.460959535303207 23.13331934608676 26-27 23.224835406094364 27.31193416069116 26.195625500506075 23.267604932708405 28-29 23.280522621482138 27.24441522847006 26.41910276442452 23.055959385623282 30-31 23.282541587352327 26.99986768206489 26.385833858456493 23.331756872126288 32-33 23.154524755004157 27.082666585165114 26.34465549949898 23.41815316033174 34-35 23.56391320825234 26.944217405503775 26.21144922448737 23.280420161756513 36-37 23.731520176111324 26.72424689553367 26.12829501964639 23.415937908708617 38-39 23.327734555953793 26.938334713744133 26.30440088352113 23.42952984678095 40-41 23.4981374074598 26.654284312133136 26.534936053609133 23.31264222679793 42-43 23.358269283373236 26.673571043880273 26.3858401469305 23.58231952581599 44-45 23.21110546912351 26.768941396728124 26.42792617951621 23.59202695463216 46-47 23.515137067209466 26.91290802362691 26.123248606274707 23.44870630288892 48-49 23.32892687202741 26.73086054805317 26.363883464199052 23.57632911572037 50-51 23.38346591823275 26.717732901918467 26.41997630434702 23.478824875501765 52-53 23.531754201389425 26.594095906318355 26.362262738871234 23.511887153420986 54-55 23.306848319772808 26.641388229807887 26.302251393530597 23.749512056888708 56-57 23.227895883848078 26.96944728629671 26.301899463490315 23.5007573663649 58-59 23.291644088570468 26.48634463642679 26.324262651411516 23.897748623591227 60-61 23.197393881927795 26.595883805838877 26.620052533688305 23.586669778545026 62-63 23.088073086828597 26.569365153805645 26.665125247915157 23.677436511450605 64-65 23.162950387609087 26.525176469766315 26.465880183076194 23.845992959548404 66-67 23.016379512302265 26.73106452529195 26.343721077784615 23.90883488462117 68-69 22.99023859963674 26.48867960079324 26.70760098450215 23.81348081506787 70-71 23.2011764420792 26.366335697729838 26.580281889389383 23.852205970801585 72-73 23.183182947149998 26.43744563565672 26.2875179467832 24.091853470410076 74-75 23.099097155752425 26.657419601623687 26.255318554331346 23.988164688292542 76-77 23.285448594949955 26.355432240886557 26.51344947607397 23.845669688089522 78-79 23.218887624563468 26.371235150847223 26.634160015858892 23.775717208730423 80-81 23.25389670742387 26.661358325475256 26.46689138692895 23.61785358017193 82-83 23.349802052228867 26.46268686697543 26.399433536623174 23.788077544172527 84-85 23.22621170376817 26.29327256138019 26.50540382338435 23.97511191146729 86-87 23.333604070134804 26.185095764410075 26.63408218259276 23.84721798286236 88-89 23.323844507266617 26.234660895643874 26.54620267922877 23.89529191786074 90-91 23.41245929298831 26.279503577501174 26.54136461168851 23.76667251782201 92-93 23.31334861460946 26.01913441664613 26.592280517595558 24.075236451148857 94-95 23.280894922104324 25.887205564728653 26.73472591274907 24.097173600417953 96-97 23.436971592138395 25.75826016447955 26.680976956750595 24.12379128663146 98-99 23.333308590830022 25.982772231575858 26.431957824802115 24.251961352792005 100-101 23.161078417716354 25.890810708373706 26.82414078134327 24.123970092566676 102-103 23.20896511385968 25.63075801873394 26.84372859693252 24.31654827047386 104-105 23.06141527917445 25.871520935409404 26.851538514944135 24.215525270472014 106-107 23.213418691611636 25.73155194064625 26.67539344740441 24.37963592033771 108-109 23.198609793663078 25.9399010831515 26.472995438385453 24.38849368479997 110-111 23.060833123385276 26.0665106719837 26.68308118462228 24.189575020008743 112-113 23.168464080576772 26.084228437964825 26.752579536575265 23.99472794488314 114-115 23.07002046197069 26.187479858975713 26.516613670482798 24.225886008570797 116-117 22.979595971592953 26.184951461397926 26.676232328560403 24.159220238448718 118-119 23.06825274691857 26.21482354809796 26.486829908404324 24.230093796579148 120-121 23.000162896393963 26.28818075298312 26.624716841958616 24.086939508664297 122-123 23.196670480649136 26.354080233380788 26.435615026508636 24.01363425946144 124-125 23.301611377891987 26.348879658897808 26.335553986914146 24.01395497629606 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 302.0 1 266.5 2 204.5 3 159.5 4 136.0 5 114.5 6 88.0 7 91.5 8 112.5 9 104.0 10 98.5 11 114.5 12 124.0 13 127.5 14 137.5 15 165.0 16 211.5 17 255.5 18 325.5 19 434.5 20 597.5 21 859.5 22 1252.5 23 1810.5 24 2650.5 25 4024.0 26 5984.0 27 8320.5 28 11420.0 29 14915.0 30 19094.5 31 24409.0 32 30656.0 33 35699.5 34 39489.5 35 44045.5 36 49978.5 37 56086.0 38 61175.0 39 65812.5 40 67946.0 41 70427.5 42 73688.0 43 77445.0 44 81878.5 45 84731.5 46 84737.0 47 85747.5 48 88184.0 49 87963.5 50 87812.0 51 86964.0 52 86567.5 53 88168.5 54 89588.0 55 84311.5 56 75186.0 57 68635.5 58 63927.5 59 57414.0 60 47230.0 61 38415.0 62 31198.0 63 24160.0 64 18544.5 65 14287.5 66 10815.0 67 8802.0 68 6546.0 69 4364.5 70 3285.0 71 2435.0 72 1855.0 73 1458.0 74 1191.0 75 912.5 76 665.5 77 433.0 78 257.5 79 163.0 80 96.5 81 59.0 82 42.5 83 27.5 84 22.0 85 16.5 86 13.5 87 12.5 88 9.0 89 5.5 90 5.0 91 6.0 92 6.5 93 5.0 94 5.5 95 4.0 96 1.0 97 1.5 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0876707735417639 2 5.67589669344089E-4 3 0.0 4 0.0026633053715376482 5 0.001702769008032267 6 3.056252065698941E-4 7 8.732148759139831E-5 8 9.605363635053814E-4 9 4.802681817526907E-4 10-11 0.008120898346000043 12-13 0.001811920867521515 14-15 0.004104109916795721 16-17 0.0022921890492742054 18-19 0.01185389194053232 20-21 0.0154559033036775 22-23 0.009168756197096822 24-25 0.008972282850016177 26-27 0.008950452478118327 28-29 0.007138531610596813 30-31 0.019560013220473224 32-33 0.0018337512394193645 34-35 0.0013534830576666737 36-37 7.640630164247352E-4 38-39 2.6196446277419494E-4 40-41 0.0013534830576666737 42-43 0.0022921890492742054 44-45 8.732148759139831E-4 46-47 0.0014189741733602224 48-49 0.008273710949284991 50-51 0.004082279544897871 52-53 0.007094870866801112 54-55 0.007029379751107564 56-57 0.00934339917227962 58-59 0.008208219833591442 60-61 0.01036942665147855 62-63 0.012617954956957058 64-65 0.008426523552569938 66-67 0.013120053510607596 68-69 0.0129017497916291 70-71 0.003994958057306473 72-73 0.012355990494182862 74-75 0.012705276444548453 76-77 0.00569772706533874 78-79 0.00825188057738714 80-81 0.012246838634693613 82-83 0.01698402933652697 84-85 0.0129017497916291 86-87 0.014844652890537711 88-89 0.014189741733602225 90-91 0.010522239254763496 92-93 0.009627194006951664 94-95 0.007706121279940901 96-97 0.008448353924467786 98-99 0.00571955743723659 100-101 0.011089828924107585 102-103 0.011591927477758127 104-105 0.009780006610236612 106-107 0.0051956285116881995 108-109 0.003471029131758083 110-111 0.006025182643806483 112-113 0.008666657643446282 114-115 0.012443311981774258 116-117 0.010958846692720488 118-119 0.21666644108615704 120-121 0.025672517351871104 122-123 0.024755641732161417 124-125 0.2324497999683023 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2290387.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.42095136606545 #Duplication Level Percentage of deduplicated Percentage of total 1 82.7115293392556 53.28355408977081 2 9.251474935853047 11.91977633813925 3 3.246322300121681 6.273935130441376 4 1.5422080486792091 3.9740203880127214 5 0.8791932952073785 2.8319234255962678 6 0.5250704428963513 2.029532247935058 7 0.3508873521601751 1.5823147933934683 8 0.2670389637787723 1.3762323278749442 9 0.19079933408745053 1.1062327159732792 >10 0.9558572265553806 10.867561973888245 >50 0.060800327092973665 2.6886400484628146 >100 0.018651899515976008 2.0098455047279753 >500 1.665347960472768E-4 0.05643101578379524 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.3098223138709747E-4 0.0 24-25 4.366074379569916E-5 0.0 0.0 5.239289255483899E-4 0.0 26-27 4.366074379569916E-5 0.0 0.0 0.0011133489667903285 0.0 28-29 6.549111569354874E-5 0.0 0.0 0.0053266107430752965 0.0 30-31 8.732148759139831E-5 0.0 0.0 0.021044478509526992 0.0 32-33 8.732148759139831E-5 0.0 0.0 0.05492521569498954 0.0 34-35 8.732148759139831E-5 0.0 0.0 0.10089997891186075 0.0 36-37 8.732148759139831E-5 0.0 0.0 0.17752458427331275 0.0 38-39 1.0915185948924789E-4 0.0 0.0 0.28968903508446386 0.0 40-41 1.3098223138709747E-4 0.0 0.0 0.41180813548103445 0.0 42-43 1.3098223138709747E-4 0.0 0.0 0.5308928141838039 0.0 44-45 1.3098223138709747E-4 0.0 0.0 0.6789900571388154 0.0 46-47 1.3098223138709747E-4 0.0 0.0 0.8589596430646873 0.0 48-49 1.3098223138709747E-4 0.0 0.0 1.0489930304354678 0.0 50-51 1.3098223138709747E-4 0.0 0.0 1.244440350036915 0.0 52-53 1.3098223138709747E-4 0.0 0.0 1.4387088295558785 0.0 54-55 1.3098223138709747E-4 0.0 0.0 1.6494810702296163 0.0 56-57 1.3098223138709747E-4 0.0 0.0 1.8945706555267736 0.0 58-59 1.3098223138709747E-4 0.0 0.0 2.1560766804911133 0.0 60-61 1.3098223138709747E-4 0.0 0.0 2.4420545523529427 0.0 62-63 1.3098223138709747E-4 0.0 0.0 2.722858626074982 0.0 64-65 1.3098223138709747E-4 0.0 0.0 3.018965790497414 0.0 66-67 1.3098223138709747E-4 0.0 0.0 3.359410440244378 0.0 68-69 1.3098223138709747E-4 0.0 0.0 3.7344998901932294 0.0 70-71 1.3098223138709747E-4 0.0 0.0 4.142465880220243 2.183037189784958E-5 72-73 1.3098223138709747E-4 0.0 0.0 4.55180718367682 4.366074379569916E-5 74-75 1.3098223138709747E-4 0.0 0.0 4.957830270604924 4.366074379569916E-5 76-77 1.3098223138709747E-4 0.0 0.0 5.399262220751341 4.366074379569916E-5 78-79 1.3098223138709747E-4 0.0 0.0 5.888153399403682 4.366074379569916E-5 80-81 1.3098223138709747E-4 0.0 0.0 6.418107507595877 4.366074379569916E-5 82-83 1.3098223138709747E-4 0.0 0.0 6.95947890029065 4.366074379569916E-5 84-85 1.3098223138709747E-4 0.0 0.0 7.50574029629054 4.366074379569916E-5 86-87 1.3098223138709747E-4 0.0 0.0 8.077914343733177 4.366074379569916E-5 88-89 1.3098223138709747E-4 0.0 0.0 8.68931756947625 4.366074379569916E-5 90-91 1.3098223138709747E-4 0.0 0.0 9.346455424345319 4.366074379569916E-5 92-93 1.3098223138709747E-4 0.0 0.0 10.019573111443613 4.366074379569916E-5 94-95 1.3098223138709747E-4 0.0 0.0 10.709434693787557 4.366074379569916E-5 96-97 1.3098223138709747E-4 0.0 0.0 11.40955655092349 4.366074379569916E-5 98-99 1.5281260328494706E-4 0.0 0.0 12.12434841797478 4.366074379569916E-5 100-101 1.7464297518279663E-4 0.0 0.0 12.91951971435395 4.366074379569916E-5 102-103 1.7464297518279663E-4 0.0 0.0 13.75468425204998 4.366074379569916E-5 104-105 1.7464297518279663E-4 0.0 0.0 14.592009996563899 4.366074379569916E-5 106-107 1.7464297518279663E-4 0.0 0.0 15.412810149551145 4.366074379569916E-5 108-109 1.7464297518279663E-4 0.0 0.0 16.258780721336613 4.366074379569916E-5 110-111 1.7464297518279663E-4 0.0 0.0 17.139199619976885 4.366074379569916E-5 112-113 1.7464297518279663E-4 0.0 0.0 18.063432075016145 4.366074379569916E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3285 0.0 52.55235 1 GGTATCA 1975 0.0 50.637432 1 TCAACGC 3825 0.0 41.829285 4 CAACGCA 3845 0.0 41.61625 5 ATCAACG 3855 0.0 41.502857 3 AACGCAG 4085 0.0 38.879147 6 GTACATG 11100 0.0 38.29171 1 TATCAAC 4355 0.0 38.10361 2 TACATGG 11520 0.0 36.24388 2 ACATGGG 11890 0.0 33.61534 3 ACGCAGA 5175 0.0 30.460218 7 CGCAGAG 5195 0.0 29.884947 8 CATGGGA 7520 0.0 29.422846 4 GAGTACA 8080 0.0 26.007154 1 AGTACAT 8130 0.0 24.873589 2 CATGGGG 5410 0.0 24.846817 4 GTATAGG 710 0.0 22.637836 1 CGTATAG 185 2.6818338E-5 22.524534 1 GCAGAGT 6605 0.0 22.514624 9 ATGGGAT 3605 0.0 22.110905 5 >>END_MODULE