FastQCFastQC Report
Thu 26 May 2016
SRR1364465_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364465_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2290387
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28850.072.072171
GTATCAA51300.056.094171
ATCAACG68700.042.302033
TCAACGC70100.041.7111474
CAACGCA70950.041.0924875
TATCAAC72550.039.5664182
AACGCAG74500.038.9738046
GTACATG110100.033.7123261
ACGCAGA87750.032.953267
TACATGG113050.032.2928962
CGCAGAG89750.031.8860638
ACATGGG114050.031.3295463
CATGGGA74700.028.778974
GCAGAGT106000.026.4365839
GAGTACA82950.025.7131121
TGGTATC14050.025.4326762
GTGGTAT15650.025.1256661
AGTACAT82800.024.5987422
AGAGTAC95300.024.44157210-11
GAGTACT50500.024.32870512-13
Produced by FastQC (version 0.11.5)