FastQCFastQC Report
Thu 26 May 2016
SRR1364464_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364464_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2694974
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19950.046.2432371
GTATCAA30250.042.4998861
ATCAACG30750.038.100783
CAACGCA31600.037.081425
GTACATG94550.035.8187261
TCAACGC32750.035.776674
AACGCAG34100.034.188416
TACATGG95950.034.028292
ACATGGG93200.033.0541953
TATCAAC37800.032.725352
CATGGGG64200.028.533874
ACGCAGA44100.026.1661827
CGCAGAG45500.025.4917968
GTATAGA6350.024.3701971
AGTACAT66150.024.3642022
GAGTACA69700.023.9956741
CTATGCG1300.00205649322.8776789
ATGGGGA40600.021.8291845
CATGGGA43300.021.4280034
GTATAGG7600.021.1450921
Produced by FastQC (version 0.11.5)