##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364464_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2694974 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.76909795790238 31.0 31.0 34.0 27.0 34.0 2 30.776675396497332 31.0 31.0 34.0 27.0 34.0 3 30.741853168156723 31.0 31.0 34.0 27.0 34.0 4 34.3479543772964 37.0 35.0 37.0 30.0 37.0 5 34.50901604245533 37.0 35.0 37.0 31.0 37.0 6 34.605966885023754 37.0 35.0 37.0 31.0 37.0 7 34.49350643085982 37.0 35.0 37.0 31.0 37.0 8 34.36308142490429 37.0 35.0 37.0 30.0 37.0 9 35.48685887136573 39.0 35.0 39.0 29.0 39.0 10-11 35.71454399931131 39.0 35.0 39.0 30.0 39.0 12-13 35.75629096978301 39.0 35.0 39.0 30.0 39.0 14-15 36.905044352932535 40.0 37.0 41.0 30.0 41.0 16-17 37.03864360101433 40.0 37.0 41.0 31.0 41.0 18-19 37.00291672572722 40.0 37.0 41.0 30.0 41.0 20-21 36.94600448835499 40.0 37.0 41.0 30.0 41.0 22-23 36.83807339143161 40.0 37.0 41.0 30.0 41.0 24-25 36.88213541206706 40.0 36.5 41.0 30.0 41.0 26-27 36.882407585379305 40.0 36.0 41.0 30.0 41.0 28-29 36.827581082414895 40.0 36.0 41.0 30.0 41.0 30-31 36.60258206572679 40.0 36.0 41.0 30.0 41.0 32-33 36.68326187933538 40.0 36.0 41.0 30.0 41.0 34-35 36.63135432846477 40.0 36.0 41.0 30.0 41.0 36-37 36.51977941160101 40.0 36.0 41.0 30.0 41.0 38-39 36.38192539148801 40.0 36.0 41.0 29.5 41.0 40-41 36.35738545158506 40.0 35.0 41.0 29.0 41.0 42-43 36.22284704787504 40.0 35.0 41.0 29.0 41.0 44-45 36.200980046560744 39.5 35.0 41.0 29.0 41.0 46-47 36.05088249459921 39.0 35.0 41.0 28.0 41.0 48-49 35.87512105868183 39.0 35.0 41.0 28.0 41.0 50-51 35.74165799002142 39.0 35.0 41.0 28.0 41.0 52-53 35.617299647417745 39.0 35.0 40.5 28.0 41.0 54-55 35.48757613245991 39.0 34.0 40.0 28.0 41.0 56-57 35.440803325004254 38.5 34.5 40.0 28.0 41.0 58-59 35.41649028896012 38.5 34.0 40.0 28.0 41.0 60-61 35.223668391606004 38.0 34.0 40.0 28.0 41.0 62-63 34.94983476649496 37.5 34.0 40.0 28.0 41.0 64-65 34.639501902430226 37.0 34.0 40.0 27.0 41.0 66-67 34.32138807276063 36.5 34.0 39.5 26.0 41.0 68-69 34.01754840677498 36.0 33.0 39.0 26.0 41.0 70-71 33.67191037835615 35.5 33.0 39.0 26.0 41.0 72-73 33.341237985969435 35.0 33.0 38.5 26.0 40.0 74-75 32.97821834273726 35.0 33.0 37.0 26.0 39.5 76-77 32.63808834519368 35.0 33.0 37.0 26.0 39.0 78-79 32.336078566991745 35.0 32.0 36.0 25.5 39.0 80-81 32.02615479778284 35.0 32.0 36.0 25.0 37.5 82-83 31.76190363988669 35.0 32.0 35.5 25.0 37.0 84-85 31.43757101181681 35.0 32.0 35.0 23.5 37.0 86-87 31.262861348569597 35.0 32.0 35.0 23.5 36.0 88-89 31.151027987654054 35.0 32.0 35.0 24.0 36.0 90-91 31.043260714203548 35.0 32.0 35.0 23.0 36.0 92-93 30.83375368370901 35.0 31.0 35.0 22.0 35.0 94-95 30.771766443757897 34.0 31.0 35.0 21.5 35.0 96-97 30.712887768119472 34.0 31.0 35.0 20.5 35.0 98-99 30.578039899457288 34.0 31.0 35.0 20.0 35.0 100-101 30.4631697745507 34.0 31.0 35.0 19.5 35.0 102-103 29.403918924635263 33.5 29.5 34.5 18.5 35.0 104-105 30.40375306032637 34.0 31.0 35.0 19.5 35.0 106-107 30.668912019188312 35.0 32.0 35.0 20.0 35.0 108-109 30.675582398939657 35.0 32.0 35.0 20.0 35.0 110-111 30.58392567052595 35.0 32.0 35.0 19.0 35.0 112-113 30.480406304476407 35.0 32.0 35.0 18.5 35.0 114-115 30.39442625420505 35.0 32.0 35.0 18.0 35.0 116-117 30.287371418054498 35.0 32.0 35.0 16.0 35.0 118-119 30.043636042499855 35.0 31.0 35.0 3.5 35.0 120-121 29.929488188012204 35.0 31.0 35.0 2.0 35.0 122-123 29.70746934849835 35.0 31.0 35.0 2.0 35.0 124-125 28.51567770227097 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5187023948875868 1101 2 0.4713766398790078 1101 3 0.6485571830881973 1101 4 0.5079560569702792 1101 5 0.4441349077250294 1101 6 0.4280442278853087 1101 7 0.503806525958808 1101 8 0.5216412914949373 1101 9 1.0683536283527673 1101 10-11 0.7870183517449618 1101 12-13 0.6873366988829659 1101 14-15 0.9398087090877851 1101 16-17 0.716290961466342 1101 18-19 0.751600352297487 1101 20-21 0.8213352146808148 1101 22-23 0.8375910186928834 1101 24-25 0.7744139572432047 1101 26-27 0.7768343293393869 1101 28-29 0.7437798721993971 1101 30-31 0.9058276466888273 1101 32-33 0.8292644118178032 1101 34-35 0.8002096507478313 1101 36-37 0.7909453137974509 1101 38-39 0.928571047330152 1101 40-41 0.8169368720639412 1101 42-43 0.7645281445910541 1101 44-45 0.5806279278989734 1101 46-47 0.662718389472559 1101 48-49 0.6933005203119507 1101 50-51 0.6355829031273146 1101 52-53 0.5901034209357618 1101 54-55 0.5459934680276675 1101 56-57 0.5559557320594735 1101 58-59 0.6704707222284512 1101 60-61 0.5918357236978409 1101 62-63 0.6008117591838058 1101 64-65 0.48991159059598743 1101 66-67 0.53848991622867 1101 68-69 0.4883343914624305 1101 70-71 0.514718859431639 1101 72-73 0.5819409100113688 1101 74-75 0.46507313050081933 1101 76-77 0.5376020854101 1101 78-79 0.5283516686018359 1101 80-81 0.5730561187080596 1101 82-83 0.5483513631371792 1101 84-85 0.6290024775897152 1101 86-87 0.6743044978322068 1101 88-89 0.4852704936200851 1101 90-91 0.47727561341044833 1101 92-93 0.6106973213564721 1101 94-95 0.553099544922464 1101 96-97 0.5581319770692978 1101 98-99 0.566885012592703 1101 100-101 0.5084162264742176 1101 102-103 0.5671259677515188 1101 104-105 0.5626048345608687 1101 106-107 0.4735011693244573 1101 108-109 0.543908785637786 1101 110-111 0.6026628208640794 1101 112-113 0.5782301334636486 1101 114-115 0.6901341813010085 1101 116-117 0.6201779935927796 1101 118-119 0.5058837780917607 1101 120-121 0.31929616841728503 1101 122-123 0.489510306921332 1101 124-125 0.14455769807366536 1102 1 0.3069424846021356 1102 2 0.2410661311276563 1102 3 0.2559575268956067 1102 4 0.2836234442061212 1102 5 0.25206682500466826 1102 6 0.1410679624010598 1102 7 0.29932879105448507 1102 8 0.3254936313773342 1102 9 0.6047255256630564 1102 10-11 0.377287040432023 1102 12-13 0.3976037644449164 1102 14-15 0.5288648495422024 1102 16-17 0.3408133692002906 1102 18-19 0.3274462832949965 1102 20-21 0.4287477934085331 1102 22-23 0.4660787702465399 1102 24-25 0.43912340555282015 1102 26-27 0.4088065739000726 1102 28-29 0.36710682369992753 1102 30-31 0.3957631028730546 1102 32-33 0.21911539171810546 1102 34-35 0.17907453296059117 1102 36-37 0.17094583024633891 1102 38-39 0.21451447650164113 1102 40-41 0.2683489294241994 1102 42-43 0.40249138080740465 1102 44-45 0.1967106999015371 1102 46-47 0.20286983196351827 1102 48-49 0.2106487765591396 1102 50-51 0.08135490815408986 1102 52-53 0.006577218532058282 1102 54-55 -0.10199416425087549 1102 56-57 0.06202917109268924 1102 58-59 0.12071056355567578 1102 60-61 -0.035201667202329645 1102 62-63 -0.04617955709687749 1102 64-65 0.0285008981363859 1102 66-67 -0.013828140054428673 1102 68-69 0.04233484151075828 1102 70-71 0.12150118605851645 1102 72-73 0.11976335242115965 1102 74-75 0.10662986733075996 1102 76-77 0.15318599237060937 1102 78-79 0.06314231005595872 1102 80-81 0.1117488389919501 1102 82-83 0.06204340711894929 1102 84-85 0.23503558442331496 1102 86-87 0.16947514730358293 1102 88-89 0.12301848131384929 1102 90-91 0.08784289563501346 1102 92-93 0.10735331474592869 1102 94-95 0.15460099712757724 1102 96-97 0.11095676809194188 1102 98-99 0.12618330609799244 1102 100-101 0.029724231678052604 1102 102-103 -0.02163081678846268 1102 104-105 0.028272295575124673 1102 106-107 -0.030337337105333262 1102 108-109 0.05529961940764139 1102 110-111 0.040186583661562025 1102 112-113 -0.03555714615408334 1102 114-115 -0.07652922681904073 1102 116-117 -0.11664770244507494 1102 118-119 0.03275879499931378 1102 120-121 -0.024196083619610675 1102 122-123 0.03464007085499787 1102 124-125 -0.25595721377342784 1103 1 0.4657402236466055 1103 2 0.5217405492324936 1103 3 0.7734283515425986 1103 4 0.5685253333505287 1103 5 0.5155476649109616 1103 6 0.3683713645803479 1103 7 0.49315199808665966 1103 8 0.6479591904910009 1103 9 1.0184846866164392 1103 10-11 0.8359364848645328 1103 12-13 0.6536709483517313 1103 14-15 0.8326345415622356 1103 16-17 0.7291776151544269 1103 18-19 0.695885321314293 1103 20-21 0.7890703879194803 1103 22-23 0.8106703153044563 1103 24-25 0.7109613990622421 1103 26-27 0.7460693562508638 1103 28-29 0.72476656278738 1103 30-31 0.9780913282808612 1103 32-33 0.8739715029605577 1103 34-35 0.8685294965316999 1103 36-37 0.7717732711971479 1103 38-39 0.8548549093499744 1103 40-41 0.8537709243091101 1103 42-43 0.8908126428160443 1103 44-45 0.8181274507450951 1103 46-47 0.6261671304225445 1103 48-49 0.679430611543772 1103 50-51 0.6866129192869295 1103 52-53 0.6140574996470605 1103 54-55 0.5054108949595033 1103 56-57 0.5340391003840921 1103 58-59 0.6505145567642074 1103 60-61 0.5566818892893224 1103 62-63 0.5579232605007505 1103 64-65 0.5873190545549605 1103 66-67 0.4929525773476229 1103 68-69 0.4767424152819544 1103 70-71 0.6166611937956219 1103 72-73 0.45087405757278987 1103 74-75 0.6187201766002417 1103 76-77 0.5544841075246012 1103 78-79 0.4640793600677853 1103 80-81 0.5938943826313974 1103 82-83 0.5603444666732074 1103 84-85 0.6421827399607238 1103 86-87 0.6507588628358292 1103 88-89 0.5685567477713249 1103 90-91 0.6522994711040759 1103 92-93 0.6076015470311695 1103 94-95 0.5576614540469187 1103 96-97 0.6065659580594875 1103 98-99 0.5544484740577573 1103 100-101 0.600927137392766 1103 102-103 0.5747896951503293 1103 104-105 0.58028179502708 1103 106-107 0.6204750594645496 1103 108-109 0.6522542068692232 1103 110-111 0.7420359679165394 1103 112-113 0.6790187097637315 1103 114-115 0.6439332804344993 1103 116-117 0.5728599164592616 1103 118-119 0.4954175669479923 1103 120-121 0.34506502235730707 1103 122-123 0.4623958790606366 1103 124-125 0.2215611081333435 1104 1 0.3900755593986993 1104 2 0.4877638645242328 1104 3 0.6459644842970178 1104 4 0.5455579866545222 1104 5 0.5548030030421742 1104 6 0.4815635373038347 1104 7 0.5343373903426496 1104 8 0.6103989692336427 1104 9 1.0268948454221203 1104 10-11 0.7711949158405673 1104 12-13 0.6942065458060824 1104 14-15 0.7805126715806736 1104 16-17 0.5979086566104428 1104 18-19 0.6654133650899468 1104 20-21 0.7541554411521005 1104 22-23 0.7807529894256646 1104 24-25 0.8225149588076732 1104 26-27 0.7231629742845271 1104 28-29 0.7043969083533028 1104 30-31 0.7958112473910788 1104 32-33 0.8038932896732192 1104 34-35 0.7022786787121547 1104 36-37 0.5356286212239212 1104 38-39 0.8044816862487991 1104 40-41 0.7591819158037865 1104 42-43 0.6958325678740351 1104 44-45 0.516237544125218 1104 46-47 0.5500757130669243 1104 48-49 0.5929930334791749 1104 50-51 0.634980254087381 1104 52-53 0.4767664153670381 1104 54-55 0.4344152368378147 1104 56-57 0.48518999552476316 1104 58-59 0.5816095427869357 1104 60-61 0.6797116876596263 1104 62-63 0.6607841419615283 1104 64-65 0.5584202857451075 1104 66-67 0.5163273468712077 1104 68-69 0.47791115375813575 1104 70-71 0.5928120889362916 1104 72-73 0.527399662759251 1104 74-75 0.5604854772604497 1104 76-77 0.5187528281574387 1104 78-79 0.44960246718727603 1104 80-81 0.510305790470575 1104 82-83 0.5451092320333402 1104 84-85 0.4069674303788524 1104 86-87 0.4766644121131769 1104 88-89 0.5240138345494145 1104 90-91 0.4039232146358671 1104 92-93 0.428470814859633 1104 94-95 0.4712551018194766 1104 96-97 0.38551528298662774 1104 98-99 0.47140261668548433 1104 100-101 0.45096133711917474 1104 102-103 0.4662085377126317 1104 104-105 0.3800409099976356 1104 106-107 0.33665896477591417 1104 108-109 0.23154404211575752 1104 110-111 0.4089633455410002 1104 112-113 0.4039042150275165 1104 114-115 0.43323025301524254 1104 116-117 0.4100647579509662 1104 118-119 0.34235297407342813 1104 120-121 0.3061249164868478 1104 122-123 0.4512229771395013 1104 124-125 0.1696503842886976 1105 1 0.3156627942411916 1105 2 0.3285281262379023 1105 3 0.2988864986516866 1105 4 0.1945643705986697 1105 5 0.12736608725059995 1105 6 0.19163182402565582 1105 7 0.23094710711379918 1105 8 0.2854844221162409 1105 9 0.7309123682918468 1105 10-11 0.36327221025564427 1105 12-13 0.42355982127438097 1105 14-15 0.04656600651671283 1105 16-17 0.22326396358860734 1105 18-19 0.18352356718519047 1105 20-21 0.5817189422930511 1105 22-23 0.641762739063779 1105 24-25 0.5733291353915249 1105 26-27 0.514071461738645 1105 28-29 0.4147938342195374 1105 30-31 0.5590655264964468 1105 32-33 0.521514668851907 1105 34-35 0.4139729718323082 1105 36-37 0.46321280441285495 1105 38-39 0.5306025764460855 1105 40-41 0.4788980152268323 1105 42-43 0.473602407949933 1105 44-45 0.4695254045859443 1105 46-47 0.5197141557580878 1105 48-49 0.33224955377977494 1105 50-51 0.33468307384355 1105 52-53 0.27094526308743383 1105 54-55 0.19232945747997832 1105 56-57 0.2756120921687426 1105 58-59 0.38958779061211146 1105 60-61 0.32648035081544435 1105 62-63 0.3554693249839289 1105 64-65 0.3426022007235474 1105 66-67 0.22120573789199227 1105 68-69 0.2728746453413393 1105 70-71 0.3697553480759481 1105 72-73 0.3594840779431081 1105 74-75 0.31304753986226075 1105 76-77 0.2936478452772775 1105 78-79 0.2510278644252253 1105 80-81 0.1889456846233557 1105 82-83 0.1792851917035314 1105 84-85 0.35925014208853767 1105 86-87 0.29781340014964996 1105 88-89 0.24669396464211601 1105 90-91 0.17052572408207212 1105 92-93 0.2022668429311416 1105 94-95 0.27660874716300654 1105 96-97 0.2134351273415689 1105 98-99 0.26211839475351795 1105 100-101 0.13190167480526327 1105 102-103 0.09388336715430867 1105 104-105 0.14337220580688026 1105 106-107 0.16830941049142112 1105 108-109 0.10905865121225133 1105 110-111 0.21871599483027282 1105 112-113 0.2073254634210464 1105 114-115 0.19525503831327384 1105 116-117 0.15728091040491066 1105 118-119 -0.009803999562048915 1105 120-121 -0.09763401068478927 1105 122-123 -0.02021805872571747 1105 124-125 -0.26340990903187134 1106 1 0.41410055465487616 1106 2 0.5331823427546531 1106 3 0.5575686782503055 1106 4 0.44938706142763607 1106 5 0.5064083394102497 1106 6 0.40935658922963114 1106 7 0.319128914258215 1106 8 0.6004234227562364 1106 9 0.996128689448156 1106 10-11 0.5138566667946236 1106 12-13 0.6178910758777718 1106 14-15 0.8467548564526197 1106 16-17 0.6889028777941277 1106 18-19 0.703079286223371 1106 20-21 0.8016222581433396 1106 22-23 0.7770296062897089 1106 24-25 0.6191250560493415 1106 26-27 0.6932698582714565 1106 28-29 0.6707389997128743 1106 30-31 0.7693223569486562 1106 32-33 0.7778695252532799 1106 34-35 0.7037244484768479 1106 36-37 0.7126708825329047 1106 38-39 0.8148167340974695 1106 40-41 0.804378518325521 1106 42-43 0.7639221945772192 1106 44-45 0.5982269060268806 1106 46-47 0.7123524763256981 1106 48-49 0.6330418881535422 1106 50-51 0.6322264620132074 1106 52-53 0.6125163802298701 1106 54-55 0.5330235369075567 1106 56-57 0.5170131287666209 1106 58-59 0.6720951884303261 1106 60-61 0.546229288276777 1106 62-63 0.7565090017112297 1106 64-65 0.7550315461063164 1106 66-67 0.675260202355247 1106 68-69 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2216 70-71 -1.5408755344473306 2216 72-73 -1.4334189893836893 2216 74-75 -1.2415807335700926 2216 76-77 -1.1564444373530094 2216 78-79 -1.2161269215021733 2216 80-81 -1.1472635300609184 2216 82-83 -1.386882850327332 2216 84-85 -1.3194734244555661 2216 86-87 -1.3537128399844711 2216 88-89 -1.753387130706681 2216 90-91 -1.5064674678376662 2216 92-93 -1.3012614595813297 2216 94-95 -1.30229616283032 2216 96-97 -1.2856106664370444 2216 98-99 -1.293787942423652 2216 100-101 -1.3594859258102012 2216 102-103 -1.619049444563398 2216 104-105 -1.4550944163062418 2216 106-107 -1.160561382353393 2216 108-109 -1.2361894468582193 2216 110-111 -1.2424555207323031 2216 112-113 -1.3910163724383011 2216 114-115 -1.2115557451259455 2216 116-117 -1.1523133137203203 2216 118-119 -1.0402293451903724 2216 120-121 -0.9676923087061091 2216 122-123 -0.9756010794446865 2216 124-125 -1.0162595225795314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40083.0 3 8042.0 4 5234.0 5 4728.0 6 5004.0 7 5849.0 8 6565.0 9 7375.0 10 7853.0 11 8526.0 12 8927.0 13 9383.0 14 9565.0 15 9251.0 16 8837.0 17 8254.0 18 7978.0 19 7735.0 20 7868.0 21 8398.0 22 9926.0 23 12904.0 24 18176.0 25 25657.0 26 31363.0 27 36087.0 28 43468.0 29 52934.0 30 64318.0 31 77416.0 32 97947.0 33 128645.0 34 175551.0 35 258773.0 36 467850.0 37 750099.0 38 257889.0 39 516.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.650455419069104 17.945721103404733 12.072421381020028 25.331402096506135 2 15.914332160653544 19.73801534490033 38.9795125506175 25.368139943828627 3 18.8711282557828 23.946279259094894 28.925956242991585 28.25663624213072 4 12.777507928797965 15.4723901202372 36.33881190668527 35.41129004427956 5 13.914326393560694 37.65733600153472 34.45085602733113 13.977481577573453 6 33.97942682764829 35.402487452531865 17.052756880791815 13.565328839028023 7 29.798729041541772 31.31009798239241 20.993226650795147 17.89794632527067 8 26.609384091097997 35.282551325741835 19.900413515086026 18.207651068074142 9 26.77147210238957 15.57780777613933 19.37570919367323 38.27501092779787 10-11 25.213712164337565 25.965285782036602 27.79954029062479 21.02146176300104 12-13 25.70601882988997 23.044304695783822 27.751016496794428 23.49865997753178 14-15 23.120207564589336 24.683287369492874 24.54359613369392 27.65290893222387 16-17 23.600809007959647 27.880959091144387 25.300757299281923 23.217474601614047 18-19 23.71287704662973 27.039309652649784 26.24154178465537 23.006271516065112 20-21 23.898371257653924 26.54498942859486 26.36505101255023 23.191588301200987 22-23 23.96835952474345 26.117679322436192 26.19735385549407 23.716607297326284 24-25 23.81451243151612 26.45387516659996 26.094317983407077 23.637294418476838 26-27 23.701309183507234 26.75363384795451 25.98225872470482 23.562798243833434 28-29 23.656767082539126 26.649705457948 26.228909892898354 23.464617566614525 30-31 23.75815951027258 26.473081896923656 26.104828819380078 23.66392977342369 32-33 23.552960389057283 26.515770664447185 26.232107801164744 23.699161145330788 34-35 23.933379827711057 26.433408121376672 25.952527125276376 23.680684925635898 36-37 23.976680880875236 26.2404469683364 25.979923234368208 23.802948916420156 38-39 23.622057178033806 26.47591242717679 26.162624906376458 23.73940548841294 40-41 23.88773837755762 26.245789790262258 26.155453726747602 23.711018105432522 42-43 23.635606987722728 26.154774689060588 26.201455491079813 24.008162832136875 44-45 23.56446966524654 26.325166649443116 26.26763318921829 23.84273049609205 46-47 23.826044203251467 26.55263804819221 25.933978060715695 23.687339687840627 48-49 23.783554610926547 26.32726199941182 26.092566427344842 23.796616962316786 50-51 23.576356496809094 26.39635066345164 26.227305753278667 23.7999870864606 52-53 23.784524953706967 26.307244720367823 26.02095153982314 23.88727878610207 54-55 23.728613862690466 26.180534076156164 26.02488045805841 24.06597160309496 56-57 23.469454590323473 26.587981857096338 26.250414004436458 23.69214954814373 58-59 23.71376014285534 26.125724277614953 26.198495298643216 23.96202028088649 60-61 23.693149115134396 26.221683799661783 26.232816751976006 23.852350333227818 62-63 23.57770584081082 26.246166931946085 26.43049561667353 23.745631610569564 64-65 23.601623818377856 26.18304156641808 26.199573653975428 24.015760961228636 66-67 23.509403531324942 26.37141480858568 26.046712976123587 24.072468683965788 68-69 23.192173344281002 26.208460321657366 26.53959948230513 24.059766851756496 70-71 23.364574935780464 26.051154838428374 26.47349627948788 24.110773946303283 72-73 23.54337955463521 26.130566364029402 26.071690333977205 24.254363747358187 74-75 23.44277057183133 26.399370307715348 26.0016005792219 24.15625854123143 76-77 23.59598343500913 26.230165798340533 26.176470588235297 23.997380178415046 78-79 23.667062248911037 26.073749741164526 26.26641981740874 23.992768192515705 80-81 23.54465123028068 26.32687097184807 26.19870861628818 23.92976918158307 82-83 23.58954308958085 26.30198770218406 26.100228109139824 24.008241099095265 84-85 23.702838197261002 26.095056358324122 26.137047234261583 24.065058210153293 86-87 23.59408287251708 25.859051738774568 26.540826609816836 24.006038778891515 88-89 23.606118854096326 26.01045539589611 26.384946575350344 23.998479174657216 90-91 23.707961071372242 26.057873398461783 26.305250169315407 23.928915360850567 92-93 23.619589556254365 25.843197604817085 26.373656550423007 24.163556288505543 94-95 23.56364236850064 25.87126616780188 26.398822169486618 24.166269294210853 96-97 23.65175115794794 25.778593934009724 26.374327371545846 24.19532753649649 98-99 23.594778251198786 25.754655437163194 26.250702276898597 24.399864034739423 100-101 23.41538584285737 25.711396463636728 26.543728609770785 24.329489083735123 102-103 23.445446789434445 25.57313834179259 26.510021037740643 24.471393831032316 104-105 23.454188087505567 25.635611421420908 26.52479850789056 24.385401983182966 106-107 23.38948860953396 25.66173727034408 26.400228289872473 24.548545830249484 108-109 23.479050450873675 25.678889478896828 26.359161209093244 24.482898861136253 110-111 23.402229743306957 25.741499027105778 26.429028261709753 24.427242967877515 112-113 23.469681979742713 25.7844187960667 26.659458781460433 24.086440442730154 114-115 23.435293654855208 25.882853498589608 26.29661888420841 24.385233962346774 116-117 23.18053282566826 25.852213056047262 26.58747388382337 24.37978023446111 118-119 23.38611327156471 25.856139263324472 26.346466680661322 24.41128078444949 120-121 23.41860042604782 25.827965980755284 26.455643277832802 24.297790315364093 122-123 23.329671857129313 25.9812450021221 26.49876951758891 24.190313623159682 124-125 23.37796136303874 26.087034142172204 26.285868584046607 24.249135910742453 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 145.0 1 141.5 2 122.0 3 98.0 4 80.5 5 76.0 6 74.0 7 71.0 8 71.5 9 73.5 10 72.5 11 76.0 12 87.5 13 95.5 14 128.5 15 157.0 16 173.0 17 215.5 18 304.5 19 382.5 20 493.5 21 730.0 22 1016.0 23 1464.0 24 2193.5 25 3361.0 26 5406.5 27 8157.5 28 10701.0 29 13748.5 30 18003.0 31 22349.0 32 27319.5 33 33404.0 34 39779.5 35 46810.0 36 55552.5 37 63160.0 38 68627.0 39 74274.5 40 77514.5 41 80424.5 42 84454.5 43 88601.0 44 94421.0 45 99080.0 46 100876.5 47 102919.0 48 104421.0 49 105796.5 50 106326.0 51 104862.0 52 107684.0 53 111657.0 54 114103.0 55 108734.0 56 96435.0 57 87097.5 58 80506.5 59 71894.0 60 59579.0 61 48424.5 62 38595.0 63 29920.0 64 23345.0 65 17794.0 66 12914.0 67 10199.0 68 7828.5 69 5174.0 70 3702.5 71 2677.0 72 1974.5 73 1584.5 74 1251.5 75 964.5 76 739.0 77 466.5 78 277.0 79 193.5 80 130.5 81 78.0 82 57.0 83 41.5 84 22.0 85 19.5 86 17.5 87 16.0 88 14.0 89 7.0 90 4.5 91 6.0 92 6.0 93 4.0 94 3.0 95 2.5 96 2.0 97 1.5 98 2.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0890175563845885 2 2.5974276560738617E-4 3 0.0 4 0.001892411577996671 5 9.276527343120935E-4 6 2.968488749798699E-4 7 0.0 8 0.0011131832811745123 9 4.452733124698049E-4 10-11 0.008460192936926294 12-13 0.0020964951795453316 14-15 0.003784823155993342 16-17 0.0025417684920151363 18-19 0.012263569147605877 20-21 0.016363794233265328 22-23 0.009684694546218257 24-25 0.010037202585256852 26-27 0.008942572358768582 28-29 0.007328456601065539 30-31 0.018386077194065695 32-33 0.0018367524139379454 34-35 9.090996796258517E-4 36-37 6.679099687047073E-4 38-39 2.0408360154866058E-4 40-41 0.0013729260467818985 42-43 0.0028200643123087645 44-45 7.606752421359167E-4 46-47 0.0013543729920956564 48-49 0.007662411585417893 50-51 0.004749581999677919 52-53 0.006827524124537008 54-55 0.007736623804162861 56-57 0.00951771705404208 58-59 0.008163344061946423 60-61 0.010055755639943095 62-63 0.012282122202292118 64-65 0.007959260460397763 66-67 0.012912926061624343 68-69 0.012486205803840779 70-71 0.004322861741894356 72-73 0.012207909983547153 74-75 0.012040932491370974 76-77 0.004972218655912822 78-79 0.007866495186966553 80-81 0.01280160773350689 82-83 0.01597418008485425 84-85 0.012653183296016954 86-87 0.014452829600582418 88-89 0.01426729905372 90-91 0.011094726702372638 92-93 0.008608617374416228 94-95 0.007013054671399428 96-97 0.008478745991612534 98-99 0.006233826374577268 100-101 0.00951771705404208 102-103 0.010872090046137737 104-105 0.009851672038394434 106-107 0.005732893898048738 108-109 0.003525080390385955 110-111 0.006660546632360831 112-113 0.008905466249396098 114-115 0.012282122202292118 116-117 0.010538135061785383 118-119 0.2171635051024611 120-121 0.025065176881112768 122-123 0.02617836016228728 124-125 0.23395401959351 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2694974.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.47831876806161 #Duplication Level Percentage of deduplicated Percentage of total 1 85.2339845653915 56.662119958101385 2 8.637879174026414 11.484633704218572 3 2.654963135738151 5.294924569651595 4 1.1586462032351164 3.0809940655227344 5 0.6066842952749243 2.0165675986431615 6 0.37711775839527 1.504209273341856 7 0.25147707739361896 1.170244131968345 8 0.16602807933295397 0.8829814065876108 9 0.11477769366855035 0.6867205296444735 >10 0.6849741039738001 8.675776930687702 >50 0.07093156394647686 3.268719610027667 >100 0.04152357990369429 4.8725692127766616 >500 0.0010127697195824535 0.39953900882822196 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.710610937248374E-5 0.0 6 3.710610937248374E-5 0.0 0.0 3.710610937248374E-5 0.0 7 1.1131832811745123E-4 0.0 0.0 3.710610937248374E-5 0.0 8 1.1131832811745123E-4 0.0 0.0 3.710610937248374E-5 0.0 9 1.1131832811745123E-4 0.0 0.0 3.710610937248374E-5 0.0 10-11 1.1131832811745123E-4 0.0 0.0 7.421221874496748E-5 0.0 12-13 1.4842443748993496E-4 0.0 0.0 7.421221874496748E-5 1.855305468624187E-5 14-15 2.5974276560738617E-4 0.0 0.0 7.421221874496748E-5 7.421221874496748E-5 16-17 2.5974276560738617E-4 0.0 0.0 7.421221874496748E-5 1.1131832811745123E-4 18-19 2.5974276560738617E-4 0.0 0.0 7.421221874496748E-5 1.1131832811745123E-4 20-21 2.5974276560738617E-4 0.0 0.0 1.2987138280369309E-4 1.1131832811745123E-4 22-23 2.5974276560738617E-4 0.0 0.0 2.0408360154866058E-4 1.1131832811745123E-4 24-25 3.710610937248374E-4 0.0 0.0 2.968488749798699E-4 1.1131832811745123E-4 26-27 3.8961414841107926E-4 0.0 0.0 6.493569140184655E-4 1.1131832811745123E-4 28-29 5.009324765285306E-4 0.0 0.0 0.003024147913857425 1.1131832811745123E-4 30-31 5.194855312147723E-4 0.0 0.0 0.013117009663173002 1.1131832811745123E-4 32-33 5.194855312147723E-4 0.0 0.0 0.033748006474273964 1.1131832811745123E-4 34-35 5.751446952734979E-4 0.0 0.0 0.0635813184097509 1.1131832811745123E-4 36-37 7.421221874496748E-4 0.0 0.0 0.11124411589870625 1.1131832811745123E-4 38-39 8.534405155671261E-4 0.0 0.0 0.18133755650332806 1.1131832811745123E-4 40-41 8.534405155671261E-4 0.0 0.0 0.257349421552861 1.1131832811745123E-4 42-43 9.090996796258517E-4 0.0 0.0 0.3325635052508855 1.1131832811745123E-4 44-45 0.0010760771718020285 0.0 0.0 0.42566273366644725 1.1131832811745123E-4 46-47 0.0012245016092919635 0.0 0.0 0.5400979749711872 1.1131832811745123E-4 48-49 0.001298713828036931 0.0 0.0 0.6620657564785412 1.1131832811745123E-4 50-51 0.001298713828036931 0.0 0.0 0.7909723804385497 1.4842443748993496E-4 52-53 0.001298713828036931 0.0 0.0 0.9213446957187713 1.4842443748993496E-4 54-55 0.001298713828036931 0.0 0.0 1.0619768502404847 1.4842443748993496E-4 56-57 0.0013358199374094147 0.0 0.0 1.2264682330887053 1.4842443748993496E-4 58-59 0.0013358199374094147 0.0 0.0 1.403111866756414 1.4842443748993496E-4 60-61 0.0013358199374094147 0.0 0.0 1.5983642142744234 1.4842443748993496E-4 62-63 0.0013729260467818985 0.0 0.0 1.7969746646906426 1.4842443748993496E-4 64-65 0.0013729260467818985 0.0 0.0 1.9978671408332698 1.4842443748993496E-4 66-67 0.0014471382655268658 0.0 0.0 2.225179166849105 1.4842443748993496E-4 68-69 0.0014471382655268658 0.0 0.0 2.481192768464557 1.4842443748993496E-4 70-71 0.0015213504842718334 0.0 0.0 2.7635517077344716 1.4842443748993496E-4 72-73 0.0016326688123892846 0.0 0.0 3.0485822868792054 1.4842443748993496E-4 74-75 0.0016697749217617684 0.0 0.0 3.3410897470624947 1.4842443748993496E-4 76-77 0.0017068810311342522 0.0 0.0 3.6581243455409957 1.4842443748993496E-4 78-79 0.0017068810311342522 0.0 0.0 4.011096210946747 1.4842443748993496E-4 80-81 0.0018924115779966708 0.0 0.0 4.39067315677257 1.4842443748993496E-4 82-83 0.002003729906114122 0.0 0.0 4.774962578488698 1.4842443748993496E-4 84-85 0.0020593890701728476 0.0 0.0 5.169456180282259 1.4842443748993496E-4 86-87 0.0021150482342315734 0.0 0.0 5.5837087853166665 1.4842443748993496E-4 88-89 0.0021150482342315734 0.0 0.0 6.03462222641109 1.4842443748993496E-4 90-91 0.002319131835780234 0.0 0.0 6.516185313847183 1.4842443748993496E-4 92-93 0.0023376848904664758 0.0 0.0 7.026672613539128 1.4842443748993496E-4 94-95 0.0023376848904664758 0.0 0.0 7.54793923800378 1.4842443748993496E-4 96-97 0.0023376848904664758 0.0 0.0 8.073992550577483 1.4842443748993496E-4 98-99 0.0025417684920151363 0.0 0.0 8.628320718493017 1.4842443748993496E-4 100-101 0.002690192929505071 0.0 0.0 9.235896153358066 1.4842443748993496E-4 102-103 0.0027829582029362805 0.0 0.0 9.876273388908391 1.4842443748993496E-4 104-105 0.003154019296661118 0.0 0.0 10.513181203232387 1.4842443748993496E-4 106-107 0.003154019296661118 0.0 0.0 11.152296831064048 1.4842443748993496E-4 108-109 0.00317257235134736 0.0 0.0 11.818036092370464 1.4842443748993496E-4 110-111 0.0032282315154060857 0.0 0.0 12.518321141502664 1.4842443748993496E-4 112-113 0.0032282315154060857 0.0 0.0 13.24591628713301 1.4842443748993496E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1995 0.0 46.243237 1 GTATCAA 3025 0.0 42.499886 1 ATCAACG 3075 0.0 38.10078 3 CAACGCA 3160 0.0 37.08142 5 GTACATG 9455 0.0 35.818726 1 TCAACGC 3275 0.0 35.77667 4 AACGCAG 3410 0.0 34.18841 6 TACATGG 9595 0.0 34.02829 2 ACATGGG 9320 0.0 33.054195 3 TATCAAC 3780 0.0 32.72535 2 CATGGGG 6420 0.0 28.53387 4 ACGCAGA 4410 0.0 26.166182 7 CGCAGAG 4550 0.0 25.491796 8 GTATAGA 635 0.0 24.370197 1 AGTACAT 6615 0.0 24.364202 2 GAGTACA 6970 0.0 23.995674 1 CTATGCG 130 0.002056493 22.877678 9 ATGGGGA 4060 0.0 21.829184 5 CATGGGA 4330 0.0 21.428003 4 GTATAGG 760 0.0 21.145092 1 >>END_MODULE