FastQCFastQC Report
Thu 26 May 2016
SRR1364464_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364464_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2694974
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29350.055.210441
GTATCAA46600.045.0004841
ATCAACG53150.038.6515123
TCAACGC54150.037.8277634
CAACGCA54400.037.623885
AACGCAG57650.035.500876
TATCAAC58300.035.341952
GTACATG90750.034.4645841
ACATGGG88000.033.765143
TACATGG92100.033.0403182
ACGCAGA70950.028.8460227
CGCAGAG73250.027.77688
CATGGGG60300.027.6497634
CATGGGA41650.024.5903154
AGTACAT64650.023.5805742
GCAGAGT87000.022.9081129
GAGTACA71200.022.1730921
GTGTACG3256.002665E-1021.996741
TATCACG3809.094947E-1221.919187
CAGAGTA85500.020.49193210-11
Produced by FastQC (version 0.11.5)