FastQCFastQC Report
Thu 26 May 2016
SRR1364463_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364463_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771666
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32550.048.4619141
CAACGCA36150.040.8063475
ATCAACG35800.040.7053763
TCAACGC36250.040.200794
AACGCAG37600.039.0745056
GTACATG100400.037.648331
TATCAAC41500.036.4044342
TACATGG103950.035.47612
ACATGGG106200.033.1562843
GGTATCA24400.031.470641
CATGGGG72350.029.1032334
ACGCAGA50400.029.03287
CGCAGAG52250.028.0048438
CATGGGA44350.022.397494
ATTCCGT4850.022.0757316
AGTACAT71700.021.900472
GAGTACA77850.021.4094311
AATACCG2858.081406E-820.870825
ATGGGGA44900.020.6662945
GCAGAGT67450.020.2829079
Produced by FastQC (version 0.11.5)