##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364463_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2771666 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.558060025991587 31.0 31.0 34.0 27.0 34.0 2 30.583532431396858 31.0 31.0 34.0 27.0 34.0 3 30.5713072931587 31.0 31.0 34.0 26.0 34.0 4 34.1832507957308 37.0 35.0 37.0 30.0 37.0 5 34.33656760951716 37.0 35.0 37.0 30.0 37.0 6 34.40738386226912 37.0 35.0 37.0 30.0 37.0 7 34.29468557899833 37.0 35.0 37.0 30.0 37.0 8 34.18274965309673 37.0 35.0 37.0 30.0 37.0 9 35.323579392322166 39.0 35.0 39.0 28.0 39.0 10-11 35.51045165615193 39.0 35.0 39.0 29.5 39.0 12-13 35.558689611230214 39.0 35.0 39.0 30.0 39.0 14-15 36.67153131004962 40.0 36.0 41.0 30.0 41.0 16-17 36.78796308790453 40.0 36.0 41.0 30.0 41.0 18-19 36.74130053909815 40.0 36.5 41.0 30.0 41.0 20-21 36.67768789601633 40.0 36.0 41.0 29.5 41.0 22-23 36.56214890250124 40.0 36.0 41.0 29.0 41.0 24-25 36.592068813486186 40.0 36.0 41.0 29.5 41.0 26-27 36.57786291710473 40.0 36.0 41.0 29.5 41.0 28-29 36.514430129748675 40.0 36.0 41.0 29.5 41.0 30-31 36.2945158254999 40.0 36.0 41.0 29.0 41.0 32-33 36.369868880305205 40.0 36.0 41.0 29.0 41.0 34-35 36.3125526308004 40.0 36.0 41.0 29.0 41.0 36-37 36.20009048709332 40.0 35.5 41.0 29.0 41.0 38-39 36.05954397102681 40.0 35.0 41.0 28.0 41.0 40-41 36.03284667055843 40.0 35.0 41.0 28.0 41.0 42-43 35.889235210880386 39.0 35.0 41.0 28.0 41.0 44-45 35.86893604784992 39.0 35.0 41.0 28.0 41.0 46-47 35.717261026400735 39.0 35.0 41.0 28.0 41.0 48-49 35.54174041893937 39.0 35.0 41.0 27.0 41.0 50-51 35.397927275508664 39.0 34.5 40.5 26.5 41.0 52-53 35.265982084421424 39.0 34.0 40.0 27.0 41.0 54-55 35.13441031495137 38.0 34.0 40.0 27.0 41.0 56-57 35.08531565491657 38.0 34.0 40.0 26.5 41.0 58-59 35.06951468899933 38.0 34.0 40.0 27.0 41.0 60-61 34.877562087206755 38.0 34.0 40.0 26.5 41.0 62-63 34.609315660689276 37.5 34.0 40.0 26.0 41.0 64-65 34.309164957105224 37.0 34.0 40.0 26.0 41.0 66-67 33.99475892838459 36.5 33.0 39.5 26.0 41.0 68-69 33.68756264282926 36.0 33.0 39.0 26.0 41.0 70-71 33.349138207850444 35.5 33.0 39.0 26.0 40.5 72-73 33.01345418243035 35.0 33.0 38.0 25.5 40.0 74-75 32.65705932821631 35.0 32.0 37.0 25.0 39.5 76-77 32.31684986574861 35.0 32.0 37.0 24.0 39.0 78-79 32.01247354479219 35.0 32.0 36.0 24.0 39.0 80-81 31.70063149744594 35.0 32.0 36.0 23.5 37.5 82-83 31.433645143390294 35.0 32.0 35.5 23.0 37.0 84-85 31.114810550766215 35.0 31.5 35.0 21.5 36.5 86-87 30.938595415176287 35.0 31.0 35.0 20.5 36.0 88-89 30.825006331931768 35.0 31.0 35.0 20.0 36.0 90-91 30.706129995461215 34.5 31.0 35.0 20.0 36.0 92-93 30.495190077015053 34.0 31.0 35.0 19.5 35.0 94-95 30.432166610262563 34.0 31.0 35.0 19.5 35.0 96-97 30.370568639944352 34.0 31.0 35.0 19.0 35.0 98-99 30.234300056356 34.0 31.0 35.0 18.0 35.0 100-101 30.122396060708613 34.0 31.0 35.0 17.5 35.0 102-103 29.06629929435942 33.5 29.0 34.5 15.0 35.0 104-105 30.0592095151436 34.0 30.5 35.0 18.0 35.0 106-107 30.32865161242372 34.5 31.0 35.0 18.0 35.0 108-109 30.334292984796868 35.0 32.0 35.0 17.5 35.0 110-111 30.24444810449744 35.0 31.5 35.0 16.0 35.0 112-113 30.147121983673358 35.0 31.0 35.0 11.5 35.0 114-115 30.058658943754406 35.0 31.0 35.0 7.0 35.0 116-117 29.946670522350097 35.0 31.0 35.0 2.0 35.0 118-119 29.696179301546437 34.0 31.0 35.0 2.0 35.0 120-121 29.579928101004953 34.0 31.0 35.0 2.0 35.0 122-123 29.36014692968056 34.0 31.0 35.0 2.0 35.0 124-125 28.150551689850076 33.5 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6269203474377534 1101 2 0.6057316296100375 1101 3 0.689992018775829 1101 4 0.5923216371890945 1101 5 0.5657242885755025 1101 6 0.5155045055433831 1101 7 0.5662375591469768 1101 8 0.6860685222317287 1101 9 1.1406209723477474 1101 10-11 0.9608451796876665 1101 12-13 0.8763108313802874 1101 14-15 1.064370244200603 1101 16-17 0.7943501631816332 1101 18-19 0.878244348088316 1101 20-21 0.8834612729841922 1101 22-23 0.9077370460631471 1101 24-25 1.0109086722326808 1101 26-27 0.9634292422897204 1101 28-29 0.858961347833386 1101 30-31 1.0385900026929775 1101 32-33 0.885143531277329 1101 34-35 0.7733253825686788 1101 36-37 0.8523007380980161 1101 38-39 0.9291280716973773 1101 40-41 0.8516348275451762 1101 42-43 0.899312403267551 1101 44-45 0.7282021599531348 1101 46-47 0.7856203135084385 1101 48-49 0.6813641871951575 1101 50-51 0.6410772011679597 1101 52-53 0.5682732525899823 1101 54-55 0.532032849138993 1101 56-57 0.4676465075333738 1101 58-59 0.6542107406988436 1101 60-61 0.5596329722992692 1101 62-63 0.623816406357399 1101 64-65 0.5472041993240637 1101 66-67 0.5745421824999966 1101 68-69 0.48119271337702685 1101 70-71 0.47482319783664906 1101 72-73 0.5269441084758668 1101 74-75 0.5080857275920891 1101 76-77 0.5263294536759133 1101 78-79 0.4446139696503124 1101 80-81 0.5672336578824186 1101 82-83 0.464608679723451 1101 84-85 0.6194638860291946 1101 86-87 0.5570616693263339 1101 88-89 0.5188191709379169 1101 90-91 0.571260518864726 1101 92-93 0.6305093969105542 1101 94-95 0.5352031605554544 1101 96-97 0.531605312099515 1101 98-99 0.5731400349466753 1101 100-101 0.5058699650350391 1101 102-103 0.5163260584359008 1101 104-105 0.5119042158916436 1101 106-107 0.4475757776629372 1101 108-109 0.5561043546840487 1101 110-111 0.5654800019043513 1101 112-113 0.5503828110150408 1101 114-115 0.5378985250136097 1101 116-117 0.5234647306074471 1101 118-119 0.5254317444935985 1101 120-121 0.4773998993889954 1101 122-123 0.4209598699401056 1101 124-125 0.1087699894980787 1102 1 0.22448011458110173 1102 2 0.0661636267192307 1102 3 0.2242005876226969 1102 4 0.23949484618961492 1102 5 0.23761116324346432 1102 6 0.1399294089319838 1102 7 0.18315918899422456 1102 8 0.2560642230237633 1102 9 0.5784879744745197 1102 10-11 0.28383428157152935 1102 12-13 0.33761892044081065 1102 14-15 0.41760543525183635 1102 16-17 0.3393126536764157 1102 18-19 0.2723626132549626 1102 20-21 0.4536594719064766 1102 22-23 0.35429562918123025 1102 24-25 0.323141354525049 1102 26-27 0.4033812587148802 1102 28-29 0.3174297142326381 1102 30-31 0.4376254011888179 1102 32-33 0.28616957121379016 1102 34-35 0.2615224069633726 1102 36-37 0.1685685051335355 1102 38-39 0.15147170495797013 1102 40-41 0.1560496052037621 1102 42-43 0.23549912932707429 1102 44-45 0.20105582662962007 1102 46-47 0.21403624999325643 1102 48-49 0.17252229038965083 1102 50-51 0.012020726067213161 1102 52-53 -0.040766102538704274 1102 54-55 -0.11721450398782451 1102 56-57 0.06968083279186743 1102 58-59 0.125662704173088 1102 60-61 0.07359224966999278 1102 62-63 0.06197209939318071 1102 64-65 0.08896311834144655 1102 66-67 0.013825738240889507 1102 68-69 -0.034625198723134076 1102 70-71 0.19051939145922603 1102 72-73 0.14519058172264465 1102 74-75 0.08197701258532675 1102 76-77 0.12220170895059823 1102 78-79 0.053530843193371425 1102 80-81 0.16875294011288133 1102 82-83 0.096694666298351 1102 84-85 0.17663681888342708 1102 86-87 0.019926196841947075 1102 88-89 0.011438680931419754 1102 90-91 -0.0064631880418346555 1102 92-93 -0.007884061924954722 1102 94-95 0.028957148687091205 1102 96-97 -0.053937150388644994 1102 98-99 -9.883350880386388E-4 1102 100-101 -0.1622347485405946 1102 102-103 -0.12751481146773358 1102 104-105 -0.04708274555652281 1102 106-107 -0.11183838703872695 1102 108-109 -0.1337667530737825 1102 110-111 -0.1804123220138223 1102 112-113 -0.1745713928950039 1102 114-115 -0.1796311317530197 1102 116-117 -0.2648135785186163 1102 118-119 -0.1826063305350729 1102 120-121 -0.3171105593910113 1102 122-123 -0.22367161283426995 1102 124-125 -0.5941075262262032 1103 1 0.4007046389925897 1103 2 0.46057686777911044 1103 3 0.6198870033119164 1103 4 0.5805559034089427 1103 5 0.5251268845282198 1103 6 0.37257539307142196 1103 7 0.4356331950577683 1103 8 0.6203482522759458 1103 9 0.9531109711639232 1103 10-11 0.7364127089482864 1103 12-13 0.6447699762578409 1103 14-15 0.9177104306089205 1103 16-17 0.7885734901895347 1103 18-19 0.6911703405414826 1103 20-21 0.7024397243600333 1103 22-23 0.7453112139255538 1103 24-25 0.5989217056594853 1103 26-27 0.7414680853101672 1103 28-29 0.6758069414203405 1103 30-31 0.8834207895390591 1103 32-33 0.7443592227336282 1103 34-35 0.8040225921608553 1103 36-37 0.7595254827324283 1103 38-39 0.7728837599338831 1103 40-41 0.8538026608256075 1103 42-43 0.7844455811482547 1103 44-45 0.6094237187706071 1103 46-47 0.5106013628325172 1103 48-49 0.5571729313916336 1103 50-51 0.5133242430153118 1103 52-53 0.4321599384693542 1103 54-55 0.539008801263833 1103 56-57 0.5654591775063054 1103 58-59 0.564169074829934 1103 60-61 0.5038247620385903 1103 62-63 0.5357988425662299 1103 64-65 0.6153334635632248 1103 66-67 0.4246152481085659 1103 68-69 0.4883567295558251 1103 70-71 0.6027847954480663 1103 72-73 0.431729171001642 1103 74-75 0.5919749159584313 1103 76-77 0.5790115776941036 1103 78-79 0.5131456542905752 1103 80-81 0.5188534114575702 1103 82-83 0.48659923013844875 1103 84-85 0.6084221745869804 1103 86-87 0.5454602421536912 1103 88-89 0.5112958806852888 1103 90-91 0.47292461877436054 1103 92-93 0.4095068704234279 1103 94-95 0.4345701769680481 1103 96-97 0.5306545827782685 1103 98-99 0.5354772822811071 1103 100-101 0.5243768809868037 1103 102-103 0.41764202141743567 1103 104-105 0.3767055230096261 1103 106-107 0.34699408427206535 1103 108-109 0.3699236109438395 1103 110-111 0.4708505061581718 1103 112-113 0.4810794812781296 1103 114-115 0.3917042063601812 1103 116-117 0.37907751497208153 1103 118-119 0.40281905100591686 1103 120-121 0.3053007146118816 1103 122-123 0.4566639950795519 1103 124-125 0.1251521375973077 1104 1 0.4389196175121661 1104 2 0.4680708077501272 1104 3 0.5900031794201794 1104 4 0.43345105334153544 1104 5 0.5430022754848167 1104 6 0.40449488102721176 1104 7 0.5877074840735474 1104 8 0.6035423098432631 1104 9 1.0721545248316247 1104 10-11 0.7997148894828143 1104 12-13 0.7889979936573539 1104 14-15 0.8610636305086459 1104 16-17 0.6612555358272374 1104 18-19 0.7231005875367487 1104 20-21 0.7931230417161999 1104 22-23 0.8362158178261367 1104 24-25 0.9255898678326346 1104 26-27 0.8500434701341106 1104 28-29 0.8337379404724672 1104 30-31 0.8260318846909342 1104 32-33 0.9246652582320039 1104 34-35 0.8935802450118402 1104 36-37 0.7408791259045842 1104 38-39 0.924296798383466 1104 40-41 0.8678694325005054 1104 42-43 0.8411305489410736 1104 44-45 0.6443037447214124 1104 46-47 0.6785356326326379 1104 48-49 0.6613144353547327 1104 50-51 0.6919219582256844 1104 52-53 0.614800147582045 1104 54-55 0.5228008462452252 1104 56-57 0.57034534148913 1104 58-59 0.532353115731496 1104 60-61 0.6543928931181142 1104 62-63 0.6695162198136515 1104 64-65 0.6156847462021133 1104 66-67 0.5374305821068077 1104 68-69 0.5549191142323693 1104 70-71 0.6907998979117806 1104 72-73 0.5631509457912713 1104 74-75 0.6080522024426074 1104 76-77 0.6427931003348846 1104 78-79 0.5173708097855894 1104 80-81 0.5637728047338157 1104 82-83 0.5410140943970312 1104 84-85 0.5390244602373251 1104 86-87 0.5469105068798576 1104 88-89 0.5867987312928022 1104 90-91 0.49513582194698813 1104 92-93 0.5681140482090221 1104 94-95 0.5508646615616399 1104 96-97 0.4380954806846695 1104 98-99 0.5093354523191174 1104 100-101 0.5067266498288383 1104 102-103 0.3621227955518407 1104 104-105 0.34945185390819233 1104 106-107 0.3512290573906043 1104 108-109 0.28377420661805886 1104 110-111 0.36788136093158386 1104 112-113 0.37532113576018844 1104 114-115 0.40277377861305297 1104 116-117 0.3623195847530596 1104 118-119 0.24061523391388917 1104 120-121 0.1425166356233163 1104 122-123 0.18012494138011093 1104 124-125 -0.014939405690071794 1105 1 0.12419125323790325 1105 2 0.14188562024284934 1105 3 0.22645045956556586 1105 4 0.07380940001386449 1105 5 -7.042128338099474E-4 1105 6 0.03663284400558098 1105 7 0.073985270452539 1105 8 0.25190544106786206 1105 9 0.6682581088101571 1105 10-11 0.19151061707980688 1105 12-13 0.3253088023183395 1105 14-15 -0.06600430859813855 1105 16-17 0.06559114713290626 1105 18-19 0.04141757926550582 1105 20-21 0.303303117114055 1105 22-23 0.3964837216181607 1105 24-25 0.3653049570855629 1105 26-27 0.29509799688440097 1105 28-29 0.16808651971464172 1105 30-31 0.33092471635001175 1105 32-33 0.23684305906957803 1105 34-35 0.1311844672576612 1105 36-37 0.27395594744010054 1105 38-39 0.4090243394997799 1105 40-41 0.22472967563491864 1105 42-43 0.2606331653464835 1105 44-45 0.16243694404633402 1105 46-47 0.220606201153295 1105 48-49 0.11721550754994325 1105 50-51 0.0988429998915521 1105 52-53 -0.10546545733881629 1105 54-55 -0.07710344564914351 1105 56-57 0.01243948516815152 1105 58-59 0.08754537642604276 1105 60-61 0.16488346540055687 1105 62-63 0.09634664799653336 1105 64-65 0.10435768763549191 1105 66-67 0.043411432382058024 1105 68-69 0.13650361011636392 1105 70-71 0.12077831880480971 1105 72-73 -0.02064418768187437 1105 74-75 0.04941382084790291 1105 76-77 0.022870716025899185 1105 78-79 -0.03844489709170418 1105 80-81 -0.03636665229755209 1105 82-83 -0.004733868287043919 1105 84-85 0.19109324169486186 1105 86-87 0.13168106119783474 1105 88-89 0.059498356691957355 1105 90-91 0.021590913562761216 1105 92-93 0.06270231782438884 1105 94-95 -0.006382934966424614 1105 96-97 0.05847923862675941 1105 98-99 0.038237999374537424 1105 100-101 -0.11684135389371164 1105 102-103 -0.1977725013160203 1105 104-105 -0.19351091333557946 1105 106-107 -0.1459853206623869 1105 108-109 -0.10805612133574627 1105 110-111 -0.1456744617593415 1105 112-113 -0.15178123310072777 1105 114-115 -0.15658705203435375 1105 116-117 -0.33632266312496384 1105 118-119 -0.37739330816248895 1105 120-121 -0.3864054774274024 1105 122-123 -0.28772935454474435 1105 124-125 -0.4872876428738664 1106 1 0.2779727966882142 1106 2 0.2481028602551838 1106 3 0.3357078893577281 1106 4 0.18421865996063502 1106 5 0.17152391728534866 1106 6 0.1389780343831859 1106 7 0.1642423793305383 1106 8 0.4187500787599703 1106 9 0.8058319138243917 1106 10-11 0.2807415871844512 1106 12-13 0.3726489902104433 1106 14-15 0.5596457523336014 1106 16-17 0.33759741649524244 1106 18-19 0.2667702177882063 1106 20-21 0.5135733666929809 1106 22-23 0.4812340063508245 1106 24-25 0.4081807150617536 1106 26-27 0.3957316450492101 1106 28-29 0.41480438582421897 1106 30-31 0.473539548037877 1106 32-33 0.48921258019433367 1106 34-35 0.48311802753814703 1106 36-37 0.4542858492990973 1106 38-39 0.5908655268402327 1106 40-41 0.557195439546966 1106 42-43 0.43395880093481765 1106 44-45 0.24668383401053262 1106 46-47 0.3906093788327709 1106 48-49 0.39713692552911084 1106 50-51 0.3028404787227572 1106 52-53 0.20437417996524943 1106 54-55 0.22544231608152643 1106 56-57 0.20980935237680143 1106 58-59 0.3141235714621544 1106 60-61 0.22264119827831763 1106 62-63 0.2853898413651521 1106 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110-111 -0.5250093707047654 2216 112-113 -0.6280655545255982 2216 114-115 -0.5452507946810883 2216 116-117 -0.44609269290847564 2216 118-119 -0.2810732761563237 2216 120-121 -0.28519153992832713 2216 122-123 -0.17115838532104277 2216 124-125 0.03722781936330577 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50947.0 3 9889.0 4 6515.0 5 5675.0 6 5743.0 7 6737.0 8 7517.0 9 8310.0 10 8908.0 11 9365.0 12 9871.0 13 10159.0 14 10289.0 15 10050.0 16 9631.0 17 8981.0 18 8526.0 19 8740.0 20 8765.0 21 9263.0 22 10995.0 23 14282.0 24 19825.0 25 26714.0 26 32708.0 27 38958.0 28 46582.0 29 55737.0 30 67569.0 31 81578.0 32 102512.0 33 134347.0 34 181859.0 35 269808.0 36 480876.0 37 749515.0 38 243671.0 39 249.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.100767044910825 18.236392545281145 12.794168999772138 24.868671410035898 2 15.8740535889207 19.89399835116564 39.001066150007844 25.23088190990582 3 18.33233152912364 24.63392053732304 29.237613767315395 27.796134166237923 4 12.651021894070539 16.023708894948726 36.77988813754276 34.54538107343797 5 13.977155796343254 37.70433720925198 34.421231586740575 13.89727540766419 6 33.18521474369266 35.738312119157776 17.555732986777205 13.52074015037236 7 29.369938058870048 31.54783552407507 21.480670095653732 17.60155632140115 8 26.80380829228658 34.59220474838688 20.134065223571927 18.469921735754625 9 26.83720490565929 15.551352693119203 19.48463301362074 38.126809387600765 10-11 25.031233993987556 25.958574658221174 28.064805779132318 20.94538556865895 12-13 25.649743605933367 23.245944373537068 27.874116601797688 23.230195418731874 14-15 23.15291429008519 24.774248437395986 24.839897018861652 27.232940253657173 16-17 23.5101184169675 27.91945964873415 25.479230532350485 23.09119140194786 18-19 23.601198434082697 27.1883997412029 26.33987579038301 22.87052603433139 20-21 24.05255185765566 26.494067933461256 26.338988891865974 23.114391317017105 22-23 24.078280404532588 26.068613074421698 26.19681561205977 23.65629090898594 24-25 23.78915394851929 26.43921702206275 26.090273828665122 23.68135520075284 26-27 23.67578562153953 26.746376410236238 25.955401056185373 23.622436912038857 28-29 23.798474953787846 26.52552430003424 26.109768255469955 23.566232490707957 30-31 23.8999887232459 26.301396062157465 26.08822030266808 23.71039491192855 32-33 23.727781914955028 26.337162146559983 26.171482629628347 23.76357330885664 34-35 24.010940828640408 26.322355041867862 25.95224878875711 23.714455340734617 36-37 23.958841830260614 26.194153733030408 25.98180722017831 23.865197216530664 38-39 23.665681817279832 26.336843745929784 26.146849233617843 23.85062520317254 40-41 23.938864112043856 26.144823134698044 26.155340415329757 23.760972337928344 42-43 23.700488418418395 26.044388095103223 26.172942045368035 24.082181441110347 44-45 23.730425218367493 26.133058454604512 26.15039472025022 23.98612160677778 46-47 23.940114715785732 26.436693897910345 25.825411557989742 23.79777982831418 48-49 23.84379921258067 26.193266108801556 26.018845311953974 23.9440893666638 50-51 23.68988377614243 26.371092977642817 26.15870264559733 23.780320600617415 52-53 23.87760381806117 26.23823769357584 25.894104874003187 23.9900536143598 54-55 23.785143400644102 26.08935363583339 25.98608633716268 24.139416626359825 56-57 23.691241470226824 26.36193571072768 26.13354896477244 23.813273854273053 58-59 23.863445291735793 26.015769385367783 25.98012012501777 24.140665197878654 60-61 23.70991009585061 26.103649568665034 26.22934434487168 23.95709599061267 62-63 23.6202612172759 26.087664086223967 26.38642338445084 23.905651312049297 64-65 23.69143222296452 26.111134063508217 26.033538924669568 24.163894788857693 66-67 23.686743549074734 26.235575978466336 25.921426080323286 24.156254392135644 68-69 23.377910213808093 26.100677042720122 26.405676434705956 24.115736308765833 70-71 23.538359818188543 25.94599957965797 26.25560944633017 24.260031155823317 72-73 23.624542861421382 26.064990807612993 25.977667641534797 24.332798689430827 74-75 23.62819031068577 26.24739248899712 25.84161127491186 24.28280592540525 76-77 23.743940654039534 26.110202071407823 26.000606160102034 24.145251114450605 78-79 23.671528611675598 26.035195940328837 26.25318605094218 24.04008939705339 80-81 23.72644575747139 26.18389381549636 26.087999828238807 24.00166059879345 82-83 23.720616037041033 26.14543462626439 26.03304636949304 24.100902967201538 84-85 23.755580634015956 26.041978455978732 26.04196041392089 24.16048049608442 86-87 23.615128841105744 25.870811213891624 26.375931064383657 24.138128880618975 88-89 23.72720562511288 25.87868031194254 26.233199835956462 24.16091422698812 90-91 23.764715603722465 26.104129218566285 26.092131392969204 24.03902378474205 92-93 23.603591933700702 25.74434443998626 26.182078434542145 24.469985191770895 94-95 23.596469293948996 25.77707321455826 26.15281420566872 24.473643285824025 96-97 23.624827728546194 25.742979560518584 26.308915754726225 24.323276956208996 98-99 23.63675430087265 25.68319014079552 26.144402231065 24.535653327266825 100-101 23.488958400913912 25.645408808526284 26.351121443697966 24.514511346861838 102-103 23.43147634161373 25.397223172416183 26.47326370696884 24.698036779001242 104-105 23.47252146132199 25.589336146852414 26.403406821364513 24.53473557046108 106-107 23.435944276796796 25.54889996303473 26.256580097459135 24.75857566270933 108-109 23.467530529962957 25.556915990713602 26.212642930631098 24.762910548692343 110-111 23.37601373701263 25.65457106924956 26.395977182674812 24.573438011063 112-113 23.313222124518703 25.69614959535228 26.595326660708235 24.39530161942078 114-115 23.42104057188983 25.754329281208154 26.25633140800555 24.56829873889647 116-117 23.151808256635434 25.666393997772225 26.571956623635018 24.609841121957327 118-119 23.259819953507694 25.656118937568767 26.304870627515935 24.779190481407596 120-121 23.19918121470955 25.735600342700334 26.50167777363156 24.563540668958552 122-123 23.228312214465827 25.896197015918638 26.367162618106804 24.50832815150873 124-125 23.283101522722394 25.95492246970748 26.199291485365432 24.562684522204687 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 201.0 1 170.5 2 127.5 3 99.5 4 76.5 5 62.5 6 66.5 7 71.5 8 74.0 9 75.5 10 74.0 11 78.5 12 78.5 13 93.0 14 109.0 15 118.0 16 146.0 17 186.5 18 244.0 19 287.0 20 360.5 21 520.0 22 734.0 23 1011.5 24 1431.5 25 2141.0 26 3280.5 27 5073.5 28 7462.0 29 10796.5 30 15344.0 31 20678.0 32 26586.0 33 32671.5 34 38890.5 35 46236.0 36 56672.5 37 66575.0 38 73340.0 39 79656.0 40 83899.5 41 87480.0 42 91044.5 43 95303.0 44 101609.0 45 106273.5 46 108535.5 47 110025.5 48 109513.0 49 109872.0 50 109869.5 51 107879.5 52 110031.0 53 112991.0 54 114348.0 55 107302.0 56 94384.5 57 85851.5 58 81856.0 59 73896.0 60 60955.5 61 50123.0 62 39685.5 63 30721.5 64 23705.0 65 18148.0 66 13760.5 67 11020.5 68 8464.0 69 5779.0 70 4089.0 71 2966.0 72 2282.5 73 1769.5 74 1294.0 75 957.0 76 722.0 77 483.5 78 302.5 79 192.0 80 138.0 81 82.5 82 47.5 83 36.0 84 30.5 85 24.0 86 21.0 87 18.0 88 16.0 89 10.0 90 7.5 91 7.5 92 8.5 93 6.0 94 2.5 95 6.0 96 7.0 97 4.0 98 4.0 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08900783860681627 2 3.9687321632548797E-4 3 0.0 4 0.0017318103985112204 5 7.937464326509759E-4 6 4.6903198293012213E-4 7 7.215876660463418E-5 8 0.0010823814990695126 9 3.607938330231709E-4 10-11 0.008893567984021163 12-13 0.0019302470066739643 14-15 0.0032651841888596965 16-17 0.002218882073092501 18-19 0.013818403804787447 20-21 0.017985572576205068 22-23 0.009741433491625614 24-25 0.010751656224090493 26-27 0.010012028866392993 28-29 0.007540591110184272 30-31 0.01726398491015873 32-33 0.0018039691651158545 34-35 0.001064341807418354 36-37 6.133495161393906E-4 38-39 2.5255568311621963E-4 40-41 0.0013349371821857324 42-43 0.0024173186812552453 44-45 7.035479743951833E-4 46-47 0.0012086593406276226 48-49 0.007486472035230796 50-51 0.004257367229673416 52-53 0.007468432343579638 54-55 0.007847265868253966 56-57 0.009759473183276773 58-59 0.00793746432650976 60-61 0.009741433491625614 62-63 0.0134034908968108 64-65 0.008280218467881772 66-67 0.013619967196624701 68-69 0.012591704772508665 70-71 0.00409501000481299 72-73 0.012663863539113298 74-75 0.012447387239299397 76-77 0.004365605379580368 78-79 0.007576670493486588 80-81 0.013583887813322384 82-83 0.016921230768786714 84-85 0.01311485583039226 86-87 0.01596512711127531 88-89 0.016253762177693847 90-91 0.011924236181415799 92-93 0.0084425756927422 94-95 0.007540591110184272 96-97 0.00867709168420726 98-99 0.005935058553231161 100-101 0.010210465474555737 102-103 0.01089597375729976 104-105 0.010282624241160372 106-107 0.0052495502704871366 108-109 0.0030847872723481114 110-111 0.006331931769556649 112-113 0.008767290142463054 114-115 0.013096816138741103 116-117 0.010643418074183541 118-119 0.21817203082911144 120-121 0.02641010857729611 122-123 0.02752856945966794 124-125 0.2376007787374092 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2771666.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.12423911663144 #Duplication Level Percentage of deduplicated Percentage of total 1 86.5541174913273 61.56095748982177 2 8.216392700429848 11.687693582030356 3 2.3520935928864324 5.018726013754543 4 0.9704599875344173 2.7609291282608432 5 0.5250863394462744 1.8673183181826765 6 0.3185659695041653 1.3594657313661467 7 0.20562003127191247 1.0237197789946917 8 0.13580658305450818 0.7727311909425206 9 0.10032907668754765 0.6422246316608394 >10 0.5423543470930636 7.216407433885158 >50 0.0510828787053965 2.492905081070643 >100 0.02759817882487722 3.3836321621160432 >500 4.928232342304216E-4 0.2132894579138761 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3.607938330231709E-5 3 0.0 0.0 0.0 0.0 3.607938330231709E-5 4 0.0 0.0 0.0 0.0 3.607938330231709E-5 5 3.607938330231709E-5 0.0 0.0 0.0 3.607938330231709E-5 6 3.607938330231709E-5 0.0 0.0 0.0 3.607938330231709E-5 7 7.215876660463418E-5 0.0 0.0 0.0 3.607938330231709E-5 8 7.215876660463418E-5 0.0 0.0 0.0 3.607938330231709E-5 9 7.215876660463418E-5 0.0 0.0 0.0 3.607938330231709E-5 10-11 7.215876660463418E-5 0.0 0.0 0.0 7.215876660463418E-5 12-13 7.215876660463418E-5 0.0 0.0 0.0 7.215876660463418E-5 14-15 7.215876660463418E-5 0.0 0.0 0.0 9.019845825579273E-5 16-17 7.215876660463418E-5 0.0 0.0 0.0 1.0823814990695128E-4 18-19 7.215876660463418E-5 0.0 0.0 0.0 1.0823814990695128E-4 20-21 7.215876660463418E-5 0.0 0.0 9.019845825579273E-5 1.0823814990695128E-4 22-23 7.215876660463418E-5 0.0 0.0 1.8039691651158544E-4 1.4431753320926836E-4 24-25 7.215876660463418E-5 0.0 0.0 1.9843660816274398E-4 1.9843660816274398E-4 26-27 7.215876660463418E-5 0.0 0.0 4.690319829301222E-4 2.1647629981390255E-4 28-29 1.623572248604269E-4 0.0 0.0 0.001623572248604269 2.1647629981390255E-4 30-31 1.8039691651158544E-4 0.0 0.0 0.007883345251556284 2.1647629981390255E-4 32-33 1.8039691651158544E-4 0.0 0.0 0.021340955223320558 2.1647629981390255E-4 34-35 1.8039691651158544E-4 0.0 0.0 0.04147325110601349 2.1647629981390255E-4 36-37 1.8039691651158544E-4 0.0 0.0 0.07526159356863345 2.1647629981390255E-4 38-39 1.8039691651158544E-4 0.0 0.0 0.12766689781524904 2.1647629981390255E-4 40-41 1.8039691651158544E-4 0.0 0.0 0.18692728488930482 2.1647629981390255E-4 42-43 1.8039691651158544E-4 0.0 0.0 0.24698141839601165 2.345159914650611E-4 44-45 1.8039691651158544E-4 0.0 0.0 0.32015040773311065 2.5255568311621963E-4 46-47 1.9843660816274398E-4 0.0 0.0 0.40751663439967156 2.5255568311621963E-4 48-49 2.1647629981390255E-4 0.0 0.0 0.5017199042020215 2.886350664185367E-4 50-51 2.1647629981390255E-4 0.0 0.0 0.6022009866989746 2.886350664185367E-4 52-53 2.1647629981390255E-4 0.0 0.0 0.704774673427462 3.066747580696953E-4 54-55 2.1647629981390255E-4 0.0 0.0 0.8188035643544352 3.247144497208538E-4 56-57 2.5255568311621963E-4 3.607938330231709E-5 0.0 0.9446845326962194 3.247144497208538E-4 58-59 2.5255568311621963E-4 3.607938330231709E-5 0.0 1.0893267803552087 3.247144497208538E-4 60-61 2.5255568311621963E-4 3.607938330231709E-5 0.0 1.2435841836642654 3.247144497208538E-4 62-63 2.5255568311621963E-4 3.607938330231709E-5 0.0 1.3997898736716472 3.607938330231709E-4 64-65 2.5255568311621963E-4 3.607938330231709E-5 0.0 1.566801338978073 3.607938330231709E-4 66-67 2.5255568311621963E-4 3.607938330231709E-5 0.0 1.7599342777953764 3.607938330231709E-4 68-69 2.5255568311621963E-4 3.607938330231709E-5 0.0 1.9754183945684654 3.607938330231709E-4 70-71 2.5255568311621963E-4 3.607938330231709E-5 0.0 2.2092849571340847 3.607938330231709E-4 72-73 2.5255568311621963E-4 3.607938330231709E-5 0.0 2.448022236445517 5.411907495347564E-4 74-75 2.5255568311621963E-4 3.607938330231709E-5 0.0 2.6970601796897604 5.411907495347564E-4 76-77 2.5255568311621963E-4 3.607938330231709E-5 0.0 2.965617069300558 5.411907495347564E-4 78-79 2.5255568311621963E-4 3.607938330231709E-5 0.0 3.2664108878919755 5.411907495347564E-4 80-81 3.247144497208538E-4 3.607938330231709E-5 0.0 3.593723053210596 5.592304411859149E-4 82-83 3.247144497208538E-4 3.607938330231709E-5 0.0 3.9254369032920993 5.772701328370734E-4 84-85 3.247144497208538E-4 3.607938330231709E-5 0.0 4.26256266086895 5.772701328370734E-4 86-87 3.247144497208538E-4 3.607938330231709E-5 0.0 4.61540098987396 5.953098244882321E-4 88-89 3.247144497208538E-4 3.607938330231709E-5 0.0 4.997265182745684 6.133495161393906E-4 90-91 3.247144497208538E-4 3.607938330231709E-5 0.0 5.413603226362772 6.133495161393906E-4 92-93 3.247144497208538E-4 3.607938330231709E-5 0.0 5.855178798599832 6.133495161393906E-4 94-95 3.247144497208538E-4 3.607938330231709E-5 0.0 6.308859003934817 6.855082827440247E-4 96-97 3.247144497208538E-4 3.607938330231709E-5 0.0 6.765623996542152 6.855082827440247E-4 98-99 3.9687321632548797E-4 3.607938330231709E-5 0.0 7.246147263054063 6.855082827440247E-4 100-101 3.9687321632548797E-4 3.607938330231709E-5 0.0 7.779292310112401 7.215876660463418E-4 102-103 3.9687321632548797E-4 3.607938330231709E-5 0.0 8.337205853807783 7.215876660463418E-4 104-105 4.1491290797664653E-4 3.607938330231709E-5 0.0 8.902605869538393 7.215876660463418E-4 106-107 4.690319829301222E-4 5.411907495347564E-5 0.0 9.471649902982538 7.576670493486589E-4 108-109 4.690319829301222E-4 7.215876660463418E-5 0.0 10.073688532456654 7.937464326509759E-4 110-111 4.690319829301222E-4 7.215876660463418E-5 0.0 10.704283993814549 8.839448909067687E-4 112-113 4.690319829301222E-4 7.215876660463418E-5 0.0 11.366268518645464 9.380639658602444E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3255 0.0 48.461914 1 CAACGCA 3615 0.0 40.806347 5 ATCAACG 3580 0.0 40.705376 3 TCAACGC 3625 0.0 40.20079 4 AACGCAG 3760 0.0 39.074505 6 GTACATG 10040 0.0 37.64833 1 TATCAAC 4150 0.0 36.404434 2 TACATGG 10395 0.0 35.4761 2 ACATGGG 10620 0.0 33.156284 3 GGTATCA 2440 0.0 31.47064 1 CATGGGG 7235 0.0 29.103233 4 ACGCAGA 5040 0.0 29.0328 7 CGCAGAG 5225 0.0 28.004843 8 CATGGGA 4435 0.0 22.39749 4 ATTCCGT 485 0.0 22.075731 6 AGTACAT 7170 0.0 21.90047 2 GAGTACA 7785 0.0 21.409431 1 AATACCG 285 8.081406E-8 20.87082 5 ATGGGGA 4490 0.0 20.666294 5 GCAGAGT 6745 0.0 20.282907 9 >>END_MODULE