FastQCFastQC Report
Thu 26 May 2016
SRR1364463_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364463_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771666
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43150.051.0800671
GGTATCA31100.047.3115351
ATCAACG53050.040.6339453
CAACGCA54350.039.6312525
TCAACGC55350.038.9454544
AACGCAG56850.037.8864026
TATCAAC58100.036.8990942
GTACATG104300.032.3839041
ACATGGG103100.031.4777493
TACATGG105500.031.440692
ACGCAGA72650.029.7287227
CGCAGAG74650.028.8509758
CATGGGG71550.026.2992424
GCAGAGT95000.022.545549
CATGGGA46150.021.9352724
AGAGTAC85700.021.06976110-11
CAGAGTA85350.020.42423610-11
AGTACAT76200.020.3192522
GAGTACA84250.019.6564641
GTATAGT8600.018.7023351
Produced by FastQC (version 0.11.5)