FastQCFastQC Report
Thu 26 May 2016
SRR1364462_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364462_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607136
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28550.043.576811
GGTATCA21600.040.7871361
CAACGCA28750.040.347075
ATCAACG29300.039.9911463
TCAACGC30550.038.160894
GTACATG83050.037.415031
AACGCAG32500.035.141196
TACATGG87100.034.8953862
ACATGGG84000.034.5545923
TATCAAC35900.033.1360552
CATGGGG55000.030.3890554
ACGCAGA38100.029.8199527
CGCAGAG39100.028.7530298
GAGTACA65150.023.0250911
CATGGGA42350.022.6123684
AGTACAT62800.022.257352
ATGGGGA34250.021.8839235
TAGACCG1500.00471385419.8286885
GCAGAGT55250.019.3797049
ATTCCGT4105.3842086E-1018.860716
Produced by FastQC (version 0.11.5)