##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364462_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2607136 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.8219160795601 31.0 31.0 34.0 27.0 34.0 2 30.880240232960613 31.0 31.0 34.0 27.0 34.0 3 30.813257919801654 31.0 31.0 34.0 27.0 34.0 4 34.4017040154407 37.0 35.0 37.0 30.0 37.0 5 34.54946193831085 37.0 35.0 37.0 31.0 37.0 6 34.691373982791845 37.0 35.0 37.0 32.0 37.0 7 34.58614356903514 37.0 35.0 37.0 31.0 37.0 8 34.45725309304923 37.0 35.0 37.0 30.0 37.0 9 35.60879140942398 39.0 35.0 39.0 30.0 39.0 10-11 35.8235535085243 39.0 35.0 39.0 30.5 39.0 12-13 35.87409402501442 39.0 35.0 39.0 30.0 39.0 14-15 37.03791190793269 40.0 37.0 41.0 30.5 41.0 16-17 37.1810484761823 40.0 37.0 41.0 31.0 41.0 18-19 37.15826082720656 40.0 37.0 41.0 31.0 41.0 20-21 37.100510675315746 40.0 37.0 41.0 30.5 41.0 22-23 37.006965689553596 40.0 37.0 41.0 30.0 41.0 24-25 37.06015700753624 40.0 37.0 41.0 30.0 41.0 26-27 37.064593676739534 40.0 37.0 41.0 30.0 41.0 28-29 37.01104871399114 40.0 37.0 41.0 30.0 41.0 30-31 36.79014117406994 40.0 36.0 41.0 30.0 41.0 32-33 36.86336213377437 40.0 36.0 41.0 30.0 41.0 34-35 36.81032673401004 40.0 36.0 41.0 30.0 41.0 36-37 36.70147337921766 40.0 36.0 41.0 30.0 41.0 38-39 36.56724620426399 40.0 36.0 41.0 30.0 41.0 40-41 36.53595056030832 40.0 36.0 41.0 30.0 41.0 42-43 36.41216933063714 40.0 35.0 41.0 29.5 41.0 44-45 36.39666611177937 40.0 35.0 41.0 29.5 41.0 46-47 36.25395990082604 39.5 35.0 41.0 29.0 41.0 48-49 36.08333646576166 39.0 35.0 41.0 28.5 41.0 50-51 35.94962594970113 39.0 35.0 41.0 28.5 41.0 52-53 35.82699751758251 39.0 35.0 41.0 28.0 41.0 54-55 35.70418420826532 39.0 35.0 40.5 28.0 41.0 56-57 35.65527805223816 39.0 34.5 40.5 28.0 41.0 58-59 35.62428273783953 39.0 35.0 41.0 28.0 41.0 60-61 35.43703761522222 38.0 34.0 40.5 28.0 41.0 62-63 35.17708646576166 38.0 34.0 40.0 28.0 41.0 64-65 34.867592638051875 37.0 34.0 40.0 28.0 41.0 66-67 34.56541085697102 37.0 34.0 40.0 28.0 41.0 68-69 34.26025052011096 36.0 34.0 39.0 27.0 41.0 70-71 33.91273470198716 36.0 33.0 39.0 26.5 41.0 72-73 33.573092849778455 35.0 33.0 38.5 26.0 40.0 74-75 33.21336190363679 35.0 33.0 37.0 26.0 39.5 76-77 32.86761622715501 35.0 33.0 37.0 26.0 39.0 78-79 32.554765267327824 35.0 33.0 36.5 26.0 39.0 80-81 32.242127760116844 35.0 32.5 36.0 26.0 38.0 82-83 31.962140064806746 35.0 32.0 36.0 25.0 37.0 84-85 31.634126297976017 35.0 32.0 35.0 24.5 37.0 86-87 31.455618732586252 35.0 32.0 35.0 24.0 36.0 88-89 31.329361989554823 35.0 32.0 35.0 24.0 36.0 90-91 31.213449547702922 35.0 32.0 35.0 24.0 36.0 92-93 31.000077479655836 35.0 32.0 35.0 23.0 35.5 94-95 30.936979505480345 35.0 32.0 35.0 23.0 35.0 96-97 30.87724633467529 35.0 32.0 35.0 23.0 35.0 98-99 30.735485797442095 34.5 31.5 35.0 21.5 35.0 100-101 30.61684507444184 34.0 31.0 35.0 20.0 35.0 102-103 29.587849080370184 33.5 29.5 34.5 19.0 35.0 104-105 30.56404096295705 34.0 31.5 35.0 21.5 35.0 106-107 30.827055435543066 35.0 32.0 35.0 23.0 35.0 108-109 30.826273351294294 35.0 32.0 35.0 23.0 35.0 110-111 30.730675921777767 35.0 32.0 35.0 20.0 35.0 112-113 30.63128620831441 35.0 32.0 35.0 19.5 35.0 114-115 30.546650999410847 35.0 32.0 35.0 19.0 35.0 116-117 30.43878263351049 35.0 32.0 35.0 18.0 35.0 118-119 30.182522699237786 35.0 32.0 35.0 8.5 35.0 120-121 30.069363661887987 35.0 31.5 35.0 2.0 35.0 122-123 29.846532171701057 35.0 31.0 35.0 2.0 35.0 124-125 28.669953926454163 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6152594096599984 1101 2 0.6295391257756471 1101 3 0.7660177113020268 1101 4 0.6297727039567604 1101 5 0.5984813224710166 1101 6 0.5583745508499902 1101 7 0.6317254020061895 1101 8 0.7129161875475916 1101 9 1.2239202644377016 1101 10-11 1.0055695596311551 1101 12-13 0.8441657759679515 1101 14-15 1.1099088970127653 1101 16-17 0.8071078622670314 1101 18-19 0.8383310027662105 1101 20-21 0.879807384274514 1101 22-23 0.8637079755182242 1101 24-25 0.9332585646573932 1101 26-27 0.7920948371739627 1101 28-29 0.7815058910347048 1101 30-31 0.9159071661510154 1101 32-33 0.8456828111506667 1101 34-35 0.7404566058837929 1101 36-37 0.6548032639698533 1101 38-39 0.9133664036310876 1101 40-41 0.8212210453860038 1101 42-43 0.7992793162558485 1101 44-45 0.6168289908723636 1101 46-47 0.6498723954818786 1101 48-49 0.6383149630100391 1101 50-51 0.6277857533732103 1101 52-53 0.5400962257119062 1101 54-55 0.5832946507307355 1101 56-57 0.6385744491523724 1101 58-59 0.7592699803221024 1101 60-61 0.6579452137563138 1101 62-63 0.6814992528465282 1101 64-65 0.6552776388436072 1101 66-67 0.7416229014502704 1101 68-69 0.6644910062537477 1101 70-71 0.6513869402550938 1101 72-73 0.609694506666763 1101 74-75 0.6848580887773608 1101 76-77 0.7260172251814154 1101 78-79 0.6672137123952169 1101 80-81 0.7138959211501898 1101 82-83 0.669929915612542 1101 84-85 0.7260083806238704 1101 86-87 0.7369820020678333 1101 88-89 0.6083057764965041 1101 90-91 0.5619845130287331 1101 92-93 0.6329487646849401 1101 94-95 0.6671624580225455 1101 96-97 0.6495272888700718 1101 98-99 0.7081912675781901 1101 100-101 0.6860746267268283 1101 102-103 0.7316239555696384 1101 104-105 0.6768320194030046 1101 106-107 0.5888983709824238 1101 108-109 0.6340260018985617 1101 110-111 0.7088472847197558 1101 112-113 0.6854273820262549 1101 114-115 0.6773874233463317 1101 116-117 0.5688168232267792 1101 118-119 0.5790145629279415 1101 120-121 0.4898795197185528 1101 122-123 0.4964458729511243 1101 124-125 0.15518689756607884 1102 1 0.23637133413812705 1102 2 0.18497303637450457 1102 3 0.2941777873327105 1102 4 0.27415964105738766 1102 5 0.2418149110224448 1102 6 0.19731082613228068 1102 7 0.24902087069666834 1102 8 0.32134741862650884 1102 9 0.5903067718536832 1102 10-11 0.40496896663393045 1102 12-13 0.2999721969581657 1102 14-15 0.44253862511366293 1102 16-17 0.2869664957483309 1102 18-19 0.27241039665969424 1102 20-21 0.4571386699214912 1102 22-23 0.48575975076263944 1102 24-25 0.37617390611421797 1102 26-27 0.31758626366892884 1102 28-29 0.32039360060893074 1102 30-31 0.3753866684244116 1102 32-33 0.3057358789599576 1102 34-35 0.26181130488780724 1102 36-37 0.26350676144353713 1102 38-39 0.2531586115390496 1102 40-41 0.2779130326585957 1102 42-43 0.37924718038151184 1102 44-45 0.29239453189219944 1102 46-47 0.33825288947338805 1102 48-49 0.2769682702922225 1102 50-51 0.16142947377247197 1102 52-53 0.2007029479945146 1102 54-55 0.14530390447978192 1102 56-57 0.12039289429081634 1102 58-59 0.20372949253685135 1102 60-61 0.18925166303657903 1102 62-63 0.17759951358760162 1102 64-65 0.2596551220717984 1102 66-67 0.1794284120275762 1102 68-69 0.189857423144268 1102 70-71 0.2918371382246647 1102 72-73 0.22846750583453712 1102 74-75 0.28064647030437584 1102 76-77 0.29696934127026964 1102 78-79 0.1844374566985465 1102 80-81 0.2729842443011279 1102 82-83 0.19797585243917837 1102 84-85 0.30565499403502727 1102 86-87 0.2141648291038294 1102 88-89 0.210803288120065 1102 90-91 0.17227647307805327 1102 92-93 0.21293833585236754 1102 94-95 0.31211023351326617 1102 96-97 0.3407450248803059 1102 98-99 0.2876981186986711 1102 100-101 0.11829572229844487 1102 102-103 0.09919607932583929 1102 104-105 0.15353114738083562 1102 106-107 0.12498506195287717 1102 108-109 0.15114482320687017 1102 110-111 0.1512512109733386 1102 112-113 0.11472862728800948 1102 114-115 0.1207733316074453 1102 116-117 0.18404289852205835 1102 118-119 0.30601012874983624 1102 120-121 0.20259819875289864 1102 122-123 0.18715972201676223 1102 124-125 -0.06627931419121325 1103 1 0.4311873542391744 1103 2 0.5058818725137684 1103 3 0.6589560380491797 1103 4 0.5627982702562235 1103 5 0.4182599802503333 1103 6 0.3233020127430919 1103 7 0.4938835897194025 1103 8 0.5843848891166772 1103 9 0.9842047172609654 1103 10-11 0.6995679686143035 1103 12-13 0.5196412739605094 1103 14-15 0.7350458990101671 1103 16-17 0.6051363988394058 1103 18-19 0.6656955019680311 1103 20-21 0.6596288439675462 1103 22-23 0.6723493815241426 1103 24-25 0.564643747881334 1103 26-27 0.7026919316339999 1103 28-29 0.6441326764444639 1103 30-31 0.8438174780979324 1103 32-33 0.6451485301320616 1103 34-35 0.6452340268302308 1103 36-37 0.6368519866186517 1103 38-39 0.631190711520027 1103 40-41 0.6933289780626737 1103 42-43 0.7692156099924148 1103 44-45 0.6216173344988078 1103 46-47 0.5564880325297921 1103 48-49 0.5145319851501569 1103 50-51 0.540619989389306 1103 52-53 0.4577261674549362 1103 54-55 0.45043480531343505 1103 56-57 0.4256659830848264 1103 58-59 0.5396685617658079 1103 60-61 0.5944324062447279 1103 62-63 0.5916605109572188 1103 64-65 0.5896907603019343 1103 66-67 0.4789091562480223 1103 68-69 0.5597656955931924 1103 70-71 0.5898290046737387 1103 72-73 0.5771850436904984 1103 74-75 0.598097574961642 1103 76-77 0.6452559197391068 1103 78-79 0.5254628589939117 1103 80-81 0.5679017872718575 1103 82-83 0.4863977698707913 1103 84-85 0.5715506756235094 1103 86-87 0.5584779367159385 1103 88-89 0.5786575938778604 1103 90-91 0.5392907043946238 1103 92-93 0.4681003249014317 1103 94-95 0.4818099325409797 1103 96-97 0.5793368219603074 1103 98-99 0.5156314130318798 1103 100-101 0.4782864011617072 1103 102-103 0.4217905920293852 1103 104-105 0.33390966172110836 1103 106-107 0.3381044353721592 1103 108-109 0.42051588490006253 1103 110-111 0.4959017069728979 1103 112-113 0.5262674699752452 1103 114-115 0.465716927941223 1103 116-117 0.5213407119043225 1103 118-119 0.4284113191061465 1103 120-121 0.34003015498750955 1103 122-123 0.477750621880741 1103 124-125 0.08288498023416935 1104 1 0.3674188156341174 1104 2 0.3793243281742136 1104 3 0.5574318812636179 1104 4 0.31799186088556297 1104 5 0.4082251293103596 1104 6 0.3906544174007678 1104 7 0.5047451367705094 1104 8 0.6238349679003932 1104 9 1.076537224547998 1104 10-11 0.753659899925097 1104 12-13 0.724734883876387 1104 14-15 0.7347302833041667 1104 16-17 0.5623574418619199 1104 18-19 0.6040246998713528 1104 20-21 0.6482839636610862 1104 22-23 0.7016829924589416 1104 24-25 0.7288053732742767 1104 26-27 0.6935108741387808 1104 28-29 0.7631342235267624 1104 30-31 0.7934498381106465 1104 32-33 0.8556959170653542 1104 34-35 0.7654338349005343 1104 36-37 0.6793085048205256 1104 38-39 0.7890633511928087 1104 40-41 0.7312193681135284 1104 42-43 0.607412882220153 1104 44-45 0.5892508366283877 1104 46-47 0.6190677974626979 1104 48-49 0.5315385186616908 1104 50-51 0.6097521755112041 1104 52-53 0.4144857110448612 1104 54-55 0.41677634296560484 1104 56-57 0.4721126323988045 1104 58-59 0.4658808977716973 1104 60-61 0.5248053201399259 1104 62-63 0.6240531314370372 1104 64-65 0.500646192790974 1104 66-67 0.492124337408967 1104 68-69 0.49522628008461567 1104 70-71 0.5818509518244497 1104 72-73 0.47202601028423175 1104 74-75 0.5514213456004526 1104 76-77 0.47962882895563297 1104 78-79 0.34487069712834284 1104 80-81 0.49242421138763603 1104 82-83 0.41526629438784113 1104 84-85 0.4093112713957474 1104 86-87 0.46804321750669686 1104 88-89 0.43864508621652476 1104 90-91 0.3757804547144623 1104 92-93 0.4013974114896932 1104 94-95 0.44450957555797643 1104 96-97 0.409078881336864 1104 98-99 0.5231794825320542 1104 100-101 0.42930644357130987 1104 102-103 0.3644571178159062 1104 104-105 0.3520656550274843 1104 106-107 0.30561538217508755 1104 108-109 0.2964678655656918 1104 110-111 0.3793854467532398 1104 112-113 0.45974098164932187 1104 114-115 0.3871154258781857 1104 116-117 0.3950002061602085 1104 118-119 0.3336217919591675 1104 120-121 0.1696827346796681 1104 122-123 0.30987282786622217 1104 124-125 0.18570344083529378 1105 1 0.12570927193574377 1105 2 0.19930671263614386 1105 3 0.3306816031621622 1105 4 0.19893826679726345 1105 5 0.15307707269408866 1105 6 0.17179199666090028 1105 7 0.311866368459107 1105 8 0.4125283704631286 1105 9 0.7346500089966241 1105 10-11 0.4013781838850292 1105 12-13 0.4495033527970236 1105 14-15 0.16775516127706425 1105 16-17 0.2733287032440188 1105 18-19 0.245601637849596 1105 20-21 0.5238023159111833 1105 22-23 0.5729248316365698 1105 24-25 0.544323336622611 1105 26-27 0.5464880884084948 1105 28-29 0.5269508761538333 1105 30-31 0.6789258489861965 1105 32-33 0.5615125789869282 1105 34-35 0.486489759464348 1105 36-37 0.48777554453937455 1105 38-39 0.5167811753191955 1105 40-41 0.5365963509010712 1105 42-43 0.5082755226039097 1105 44-45 0.4437283801193459 1105 46-47 0.5056225215674601 1105 48-49 0.45975526217925733 1105 50-51 0.4644363383548935 1105 52-53 0.36363356367115784 1105 54-55 0.45982568995305684 1105 56-57 0.42785218659507507 1105 58-59 0.5567702989999432 1105 60-61 0.5223800039554547 1105 62-63 0.5180254841900265 1105 64-65 0.5826984127596901 1105 66-67 0.4189037342247701 1105 68-69 0.405061605866905 1105 70-71 0.5164292966976518 1105 72-73 0.37529170895238195 1105 74-75 0.35803351954668017 1105 76-77 0.31512925014359183 1105 78-79 0.2582107861012446 1105 80-81 0.31586244057844937 1105 82-83 0.33899440215325427 1105 84-85 0.4524560093835781 1105 86-87 0.40079384635352255 1105 88-89 0.36546771472287887 1105 90-91 0.28191344786881345 1105 92-93 0.23828357040229875 1105 94-95 0.27092950728737364 1105 96-97 0.2575486873947348 1105 98-99 0.23007889345593924 1105 100-101 0.1156807686188337 1105 102-103 0.09715887711946536 1105 104-105 0.20028151312089548 1105 106-107 0.20945177584739127 1105 108-109 0.2164752351319521 1105 110-111 0.2153552405814878 1105 112-113 0.19660050445951427 1105 114-115 0.20252106020877036 1105 116-117 0.1364773260172889 1105 118-119 0.05070627120746707 1105 120-121 0.078537428748497 1105 122-123 0.1733952114867563 1105 124-125 -0.0655099816079101 1106 1 0.38923721383076426 1106 2 0.43483821583283344 1106 3 0.49703575817024515 1106 4 0.3182195622965551 1106 5 0.31060285552223377 1106 6 0.2649680091764921 1106 7 0.2504406324214301 1106 8 0.4599884592811989 1106 9 0.8186531921287852 1106 10-11 0.32324621184689306 1106 12-13 0.5450606129367301 1106 14-15 0.7194649731241682 1106 16-17 0.5737639802963201 1106 18-19 0.48912828918568607 1106 20-21 0.61503826829437 1106 22-23 0.6242975204020667 1106 24-25 0.507046891751294 1106 26-27 0.5585672947061298 1106 28-29 0.4869866296898664 1106 30-31 0.6037392664601882 1106 32-33 0.5545296629302072 1106 34-35 0.47129444501911166 1106 36-37 0.532451409632948 1106 38-39 0.647661322948295 1106 40-41 0.6104610966276809 1106 42-43 0.5693981252088918 1106 44-45 0.30623661405786606 1106 46-47 0.4606354185283479 1106 48-49 0.34893726599447916 1106 50-51 0.41837281425879524 1106 52-53 0.3507961694119359 1106 54-55 0.18360004108664896 1106 56-57 0.17077814869628583 1106 58-59 0.28122395006140977 1106 60-61 0.2400727964488638 1106 62-63 0.2685124637200573 1106 64-65 0.310620887763271 1106 66-67 0.2734863709192368 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-1.618799100923809 2216 68-69 -1.1643273789404773 2216 70-71 -1.1295010729707542 2216 72-73 -1.13143051831819 2216 74-75 -0.999587402655493 2216 76-77 -0.9735978754605874 2216 78-79 -0.913800667864372 2216 80-81 -0.8469836773190735 2216 82-83 -1.0419154568249347 2216 84-85 -1.103273894250794 2216 86-87 -1.1269539478818444 2216 88-89 -1.52669084070142 2216 90-91 -1.2202181602298836 2216 92-93 -1.0265184192211976 2216 94-95 -1.0013165863781133 2216 96-97 -0.9700408954247521 2216 98-99 -0.9985222688165045 2216 100-101 -1.145013674022632 2216 102-103 -1.4214569001015533 2216 104-105 -1.290742409526974 2216 106-107 -1.0011243733354291 2216 108-109 -0.9699828953662362 2216 110-111 -0.9466603890837177 2216 112-113 -1.2013194346142164 2216 114-115 -1.098383584553865 2216 116-117 -1.001698574787131 2216 118-119 -0.81134159572402 2216 120-121 -0.779631526980431 2216 122-123 -0.7549798572067488 2216 124-125 -0.7508757772193135 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33176.0 3 6862.0 4 4535.0 5 3986.0 6 4460.0 7 5302.0 8 6046.0 9 6650.0 10 7471.0 11 8319.0 12 8775.0 13 9112.0 14 9314.0 15 8859.0 16 8491.0 17 7940.0 18 7559.0 19 7422.0 20 7388.0 21 8012.0 22 9277.0 23 12338.0 24 17594.0 25 24910.0 26 30020.0 27 33809.0 28 40881.0 29 49189.0 30 59915.0 31 73306.0 32 92595.0 33 122166.0 34 168435.0 35 247489.0 36 445040.0 37 735247.0 38 274645.0 39 601.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.43132760927443 18.117203081240557 12.314136045255001 25.137333264230005 2 15.86092753334126 19.896092638265063 39.16486711441316 25.078112713980506 3 18.677736796239245 24.1053017564101 29.5031022547347 27.71385919261596 4 12.680426329902813 15.944910204446415 37.04135148435669 34.33331198129408 5 13.642774429454235 37.934777563960694 34.84173465704733 13.580713349537746 6 33.282869176884475 36.221770214078234 17.262653852069572 13.232706756967715 7 29.24903035361408 32.05448430768475 21.182247493034502 17.514237845666663 8 26.43265230974587 35.30153177566688 20.113382468293377 18.152433446293877 9 26.700903757948446 15.730305617629304 19.688820665302707 37.87996995911955 10-11 24.95836497237406 26.104889006185367 28.186937677786794 20.749808343653783 12-13 25.53121181673837 23.44905596545409 27.876650576516308 23.14308164129123 14-15 22.74735582948809 25.13146773399473 24.92161034380977 27.199566092707407 16-17 23.257972668861473 28.305683445317587 25.6902115522333 22.746132333587642 18-19 23.246819482888686 27.428777266267694 26.65112891646154 22.673274334382075 20-21 23.684649681088832 26.959924532413236 26.595227189200678 22.760198597297254 22-23 23.676864515391525 26.468981612723674 26.552415418260377 23.30173845362442 24-25 23.443232787432354 26.741386403718657 26.508212489966347 23.30716831888264 26-27 23.284074819543367 27.138445155645165 26.336541408645424 23.240938616166044 28-29 23.252670255457268 27.085593749082964 26.482696875059336 23.17903912040043 30-31 23.433797315240046 26.803059418661352 26.456521213716204 23.306622052382398 32-33 23.252077706829976 26.82785843631249 26.512774248287645 23.407289608569894 34-35 23.78884677316039 26.747687662392956 26.165796351657892 23.29766921278876 36-37 23.740617971149685 26.61813148035067 26.164372685178083 23.476877863321562 38-39 23.266396305204925 26.796489008782466 26.43219912186863 23.504915564143978 40-41 23.597528196727033 26.40900208643026 26.51918263485514 23.474287081987562 42-43 23.333980933699085 26.424031970105794 26.521459705288773 23.720527390906348 44-45 23.31369022082152 26.67452467880567 26.490489165091326 23.521295935281483 46-47 23.703544451301816 26.922843830801323 26.031121300423578 23.342490417473282 48-49 23.517255096241836 26.652112962674202 26.263739277719694 23.56689266336427 50-51 23.31848429746582 26.741728232916145 26.473413756074716 23.466373713543323 52-53 23.517075852143154 26.62698265045396 26.291568578636838 23.56437291876604 54-55 23.443291099424222 26.49884345834724 26.239436727773498 23.818428714455045 56-57 23.255843392870602 26.92990728864998 26.395757799294127 23.418491519185284 58-59 23.44041713929078 26.52599889754072 26.269813291577464 23.763770671591036 60-61 23.266663687346284 26.54998343791963 26.588343786208572 23.59500908852552 62-63 23.218434942593642 26.62290108782258 26.68769399029526 23.470969979288522 64-65 23.240973581734092 26.54189983886927 26.434760660166074 23.78236591923056 66-67 23.137205783740402 26.696840824380956 26.353962549621063 23.81199084225758 68-69 23.035725725675807 26.340649775565765 26.853102509343913 23.770521989414515 70-71 23.22747985196099 26.278727533457317 26.61978802964539 23.8740045849363 72-73 23.169085201939943 26.460524536520662 26.304182079214662 24.06620818232473 74-75 23.228592105346443 26.63477430763013 26.232152615314742 23.904480971708686 76-77 23.362700523323632 26.49794341445751 26.456535523137592 23.682820539081263 78-79 23.37472208455381 26.363224172084326 26.494452431769744 23.767601311592124 80-81 23.36295492122435 26.618132785017472 26.415046206608178 23.603866087149996 82-83 23.390721312324427 26.56772187530271 26.380222294898957 23.661334517473907 84-85 23.32109470214706 26.358547522166297 26.486809553421033 23.833548222265613 86-87 23.329800793526726 26.252748907390234 26.69583489580286 23.72161540328018 88-89 23.367516790263927 26.32974632094982 26.596115653432534 23.706621235353715 90-91 23.50574302577783 26.40217138622088 26.51660225854242 23.575483329458873 92-93 23.366037234996455 26.025657918838085 26.648223131484418 23.960081714681042 94-95 23.264739187273296 26.197712403257782 26.669647186266747 23.867901223202175 96-97 23.43780028654889 25.98607149912635 26.656315871240967 23.919812343083795 98-99 23.360511028380174 26.047740708256704 26.452711443535108 24.13903681982801 100-101 23.30456037679249 25.99746973929415 26.658268267477247 24.03970161643611 102-103 23.206405237984903 25.662727232273696 26.826873059884825 24.303994469856573 104-105 23.046450188867283 25.988249451350804 26.750450830252674 24.214849529529236 106-107 23.24059318456474 25.940710312451632 26.468505026602624 24.350191476381 108-109 23.265279820056566 25.95468848501261 26.43068781221933 24.349343882711494 110-111 23.18620155276644 26.096641298677387 26.646803173044464 24.070353975511708 112-113 23.14323860531604 26.146363599921667 26.790465783965494 23.919932010796796 114-115 23.086844005177294 26.25772700733399 26.526086346070187 24.129342641418525 116-117 22.97798450873256 26.14709051073078 26.80367504742841 24.071249933108245 118-119 22.95822291657096 26.218531102592777 26.546806936559285 24.27643904427697 120-121 23.000248616495245 26.377346893675014 26.60123602299549 24.021168466834254 122-123 23.10004343108653 26.36512499098383 26.549956492180105 23.98487508574954 124-125 23.09122282773772 26.431808762023696 26.47141034700985 24.005558063228737 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 338.0 1 305.0 2 249.0 3 201.0 4 163.0 5 139.5 6 130.5 7 130.0 8 117.0 9 101.5 10 121.5 11 135.0 12 115.0 13 113.5 14 162.5 15 211.5 16 226.5 17 257.5 18 340.5 19 456.5 20 613.5 21 879.5 22 1329.5 23 1966.0 24 2953.5 25 4598.5 26 6888.5 27 10178.0 28 13744.0 29 17274.5 30 21378.5 31 26288.0 32 32937.0 33 38604.0 34 42509.5 35 47707.5 36 56077.0 37 63616.5 38 68245.5 39 73244.5 40 77023.5 41 81134.5 42 85522.0 43 89464.5 44 94065.5 45 98361.0 46 99511.5 47 99250.0 48 99857.5 49 99507.5 50 97959.0 51 96071.0 52 96699.5 53 99294.0 54 103139.5 55 98661.5 56 87723.0 57 80832.5 58 75162.0 59 65945.5 60 53736.5 61 44398.5 62 37111.0 63 29211.0 64 22490.0 65 17010.0 66 11745.0 67 8878.0 68 6587.0 69 4156.5 70 3098.0 71 2312.0 72 1723.0 73 1317.5 74 1020.5 75 802.5 76 573.5 77 316.0 78 188.5 79 128.0 80 73.0 81 35.0 82 27.0 83 19.5 84 15.5 85 12.0 86 11.0 87 11.0 88 7.5 89 8.0 90 6.5 91 4.0 92 5.5 93 6.5 94 5.0 95 2.5 96 2.5 97 3.0 98 2.0 99 4.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08979201698722276 2 2.3013759159476143E-4 3 0.0 4 0.0018027444674922981 5 0.0010356191621764266 6 1.917813263289679E-4 7 0.0 8 0.0011890442232396008 9 8.438378358474588E-4 10-11 0.009838382040676052 12-13 0.00209041645698575 14-15 0.004890423821388681 16-17 0.002800007364402931 18-19 0.014057571219913346 20-21 0.017413744430670283 22-23 0.010701398009156407 24-25 0.010874001202852479 26-27 0.01062468547862482 28-29 0.008534269021639071 30-31 0.019063063837099405 32-33 0.0021095945896186467 34-35 0.0013041130190369816 36-37 9.205503663790457E-4 38-39 3.452063873921422E-4 40-41 0.0019753476611883694 42-43 0.002895898027567415 44-45 0.001169866090606704 46-47 0.0018027444674922981 48-49 0.009186325531157561 50-51 0.004621929964528126 52-53 0.008016459440550858 54-55 0.008668515950069348 56-57 0.010797288672320892 58-59 0.009090434867993078 60-61 0.010643863611257718 62-63 0.01415346188307783 64-65 0.009454819388018116 66-67 0.014575380801001559 68-69 0.013769899230419894 70-71 0.004621929964528126 72-73 0.013750721097786998 74-75 0.013290445914597476 76-77 0.005561658463540068 78-79 0.008745228480600937 80-81 0.014038393087280448 82-83 0.017778128950695322 84-85 0.01392332429148307 86-87 0.016052097013734612 88-89 0.01599456261583592 90-91 0.012350717415585532 92-93 0.009991807101739227 94-95 0.007978103175285063 96-97 0.009627422581714187 98-99 0.006041111779362488 100-101 0.011123316927080136 102-103 0.012734280068243467 104-105 0.010701398009156407 106-107 0.006002755514096695 108-109 0.0036246670676174926 110-111 0.00675070268677967 112-113 0.009646600714347083 114-115 0.014191818148343624 116-117 0.011986332895560493 118-119 0.22313757318375413 120-121 0.027309660869245026 122-123 0.027156235808181852 124-125 0.23895953260589398 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2607136.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.24164094078225 #Duplication Level Percentage of deduplicated Percentage of total 1 84.3145882329968 56.694512680330746 2 9.08281096863591 12.214862277720288 3 2.9437914179751288 5.938360965961195 4 1.2668831784897425 3.4074921520769683 5 0.6800069362603081 2.2862391122628516 6 0.38210727812407685 1.5416112237887274 7 0.2605680842017746 1.226471789096627 8 0.1818838337898479 0.9784133955703895 9 0.12894978128702997 0.7803715403425383 >10 0.662996428083311 8.31605241783841 >50 0.06462179973097835 2.9742980300674926 >100 0.030506948955921053 3.5300246525279717 >500 2.851114691188765E-4 0.11128976241579738 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.8356265265793574E-5 0.0 0.0 0.0 0.0 3 3.8356265265793574E-5 0.0 0.0 0.0 0.0 4 3.8356265265793574E-5 0.0 0.0 0.0 0.0 5 3.8356265265793574E-5 0.0 0.0 0.0 0.0 6 7.671253053158715E-5 0.0 0.0 0.0 0.0 7 1.1506879579738073E-4 0.0 0.0 0.0 0.0 8 1.1506879579738073E-4 0.0 0.0 0.0 0.0 9 1.1506879579738073E-4 0.0 0.0 0.0 0.0 10-11 1.1506879579738073E-4 0.0 0.0 0.0 0.0 12-13 1.342469284302775E-4 0.0 0.0 1.9178132632896787E-5 0.0 14-15 2.3013759159476146E-4 0.0 0.0 3.8356265265793574E-5 0.0 16-17 2.4931572422765827E-4 0.0 0.0 3.8356265265793574E-5 0.0 18-19 3.6438452002503897E-4 0.0 0.0 3.8356265265793574E-5 0.0 20-21 4.602751831895229E-4 0.0 0.0 7.671253053158715E-5 0.0 22-23 5.753439789869037E-4 0.0 0.0 7.671253053158715E-5 0.0 24-25 9.205503663790458E-4 0.0 0.0 2.1095945896186467E-4 0.0 26-27 9.205503663790458E-4 0.0 0.0 7.671253053158716E-4 0.0 28-29 0.0012274004885053944 0.0 0.0 0.003336995078124041 0.0 30-31 0.0013808255495685686 0.0 0.0 0.012695923802977672 0.0 32-33 0.0014000036822014654 0.0 0.0 0.034885023259239256 0.0 34-35 0.0015534287432646399 0.0 0.0 0.06779469885729014 0.0 36-37 0.0017260319369607109 0.0 0.0 0.12233730806524862 0.0 38-39 0.0019369913959225756 0.0 0.0 0.1993375105863292 0.0 40-41 0.0020328820590870596 0.0 0.0 0.2794829268592049 0.0 42-43 0.002071238324352853 0.0 0.0 0.36263930995544535 0.0 44-45 0.0022054852527831304 0.0 0.0 0.46311354681919165 0.0 46-47 0.002473979109643686 0.0 0.0 0.5853933204865416 0.0 48-49 0.002608226038073963 0.0 0.0 0.7167251727566187 0.0 50-51 0.002608226038073963 0.0 0.0 0.851010457452162 0.0 52-53 0.002608226038073963 0.0 0.0 0.9859094423919581 0.0 54-55 0.0026465823033397567 0.0 0.0 1.1373399776613111 0.0 56-57 0.0028767198949345182 0.0 0.0 1.3156774330146184 0.0 58-59 0.0028767198949345182 0.0 0.0 1.5051382052950057 0.0 60-61 0.0029150761602003117 0.0 0.0 1.7129716286377081 0.0 62-63 0.0029534324254661052 0.0 0.0 1.916643397199072 0.0 64-65 0.0029534324254661052 0.0 0.0 2.129539847556859 0.0 66-67 0.0031068574865292797 0.0 0.0 2.375767892430621 0.0 68-69 0.0031452137517950732 0.0 0.0 2.6526234151191193 0.0 70-71 0.0032027481496937635 0.0 0.0 2.9493091269500322 0.0 72-73 0.0032986388128582473 0.0 0.0 3.2500414247664873 0.0 74-75 0.003356173210756938 0.0 0.0 3.568149110748346 0.0 76-77 0.0033753513433898348 0.0 0.0 3.9072568519632274 0.0 78-79 0.0033753513433898348 0.0 0.0 4.281614000957372 0.0 80-81 0.0041232985160728096 0.0 0.0 4.690664391884428 0.0 82-83 0.004142476648705706 0.0 0.0 5.113887422827194 0.0 84-85 0.004219189179237293 0.0 0.0 5.54491595375155 0.0 86-87 0.004315079842401777 0.0 0.0 5.989081505529439 0.0 88-89 0.004334257975034674 0.0 0.0 6.469858112503529 0.0 90-91 0.004602751831895229 0.0 0.0 6.98308411989248 0.0 92-93 0.00471782062769261 0.0 0.0 7.524866366771814 0.0 94-95 0.00471782062769261 0.0 0.0 8.068355467455476 0.0 96-97 0.00471782062769261 0.0 0.0 8.628299405938163 0.0 98-99 0.004928780086654474 0.0 0.0 9.213616013894175 0.0 100-101 0.005101383280350546 0.0 0.0 9.861683471825021 0.0 102-103 0.005331520871945307 0.0 0.0 10.53537291495342 0.0 104-105 0.0059835773814637975 0.0 0.0 11.207777423195415 0.0 106-107 0.006175358707792766 0.0 0.0 11.878781927755206 0.0 108-109 0.00632878376885594 0.0 0.0 12.58718379094915 0.0 110-111 0.006482208829919114 0.0 0.0 13.329204920648557 0.0 112-113 0.006520565095184908 0.0 0.0 14.099859002368884 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2855 0.0 43.57681 1 GGTATCA 2160 0.0 40.787136 1 CAACGCA 2875 0.0 40.34707 5 ATCAACG 2930 0.0 39.991146 3 TCAACGC 3055 0.0 38.16089 4 GTACATG 8305 0.0 37.41503 1 AACGCAG 3250 0.0 35.14119 6 TACATGG 8710 0.0 34.895386 2 ACATGGG 8400 0.0 34.554592 3 TATCAAC 3590 0.0 33.136055 2 CATGGGG 5500 0.0 30.389055 4 ACGCAGA 3810 0.0 29.819952 7 CGCAGAG 3910 0.0 28.753029 8 GAGTACA 6515 0.0 23.025091 1 CATGGGA 4235 0.0 22.612368 4 AGTACAT 6280 0.0 22.25735 2 ATGGGGA 3425 0.0 21.883923 5 TAGACCG 150 0.004713854 19.828688 5 GCAGAGT 5525 0.0 19.379704 9 ATTCCGT 410 5.3842086E-10 18.86071 6 >>END_MODULE