FastQCFastQC Report
Thu 26 May 2016
SRR1364462_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364462_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607136
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31700.054.876331
GTATCAA49750.053.2878721
ATCAACG59500.044.7354623
CAACGCA60300.044.306295
TCAACGC60800.043.8768844
TATCAAC63250.042.0855832
AACGCAG65750.040.6314166
GTACATG82150.035.4619641
ACGCAGA78750.033.9240077
CGCAGAG80350.033.1718868
TACATGG89200.032.3122252
ACATGGG86600.032.111483
CATGGGG58100.028.1849924
GCAGAGT95500.027.348869
GAGTACT52000.024.31272512-13
CCGTTGC4150.022.9377029
AGAGTAC87800.022.93729610-11
CAGAGTA92550.022.85289610-11
GTACTTT57650.022.03315414-15
GAGTACA68350.021.0929991
Produced by FastQC (version 0.11.5)