FastQCFastQC Report
Thu 26 May 2016
SRR1364461_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364461_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320161
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.052.1906851
GTATCAA30800.050.2405171
ATCAACG35550.040.4888733
CAACGCA35500.040.214285
TCAACGC35850.039.9841464
GTACATG99750.039.3788031
AACGCAG38150.037.4208876
TACATGG105450.036.6064872
ACATGGG103400.035.8941763
TATCAAC43250.034.243112
ACGCAGA46550.030.9238177
CATGGGG59050.029.2104364
CGCAGAG49450.028.8697038
CATGGGA54250.028.2865454
TATAGGG6000.023.7913972
GAGTACA71700.023.6568621
AGTACAT70100.023.1635232
ATGGGGA32600.022.2607425
GTATAGG9400.022.1600951
GCAGAGT64150.021.6051189
Produced by FastQC (version 0.11.5)