FastQCFastQC Report
Thu 26 May 2016
SRR1364461_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364461_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320161
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.071.147711
GTATCAA50600.053.7973251
ATCAACG65200.041.095223
TCAACGC66200.040.4744454
CAACGCA66900.039.9335175
AACGCAG69800.038.3596346
TATCAAC71250.037.772862
GTACATG98800.035.2690621
TACATGG103050.033.1657832
ACATGGG101300.033.0333943
ACGCAGA82700.032.304147
CGCAGAG85200.031.3535468
CATGGGG59550.027.3965034
GTATAGA5700.027.170241
GCAGAGT102550.025.816919
CATGGGA53950.025.7152544
CAGAGTA93900.024.61531410-11
AGAGTAC90600.024.39554610-11
GTACTTT52400.024.01369514-15
GAGTACA73650.023.858561
Produced by FastQC (version 0.11.5)