FastQCFastQC Report
Thu 26 May 2016
SRR1364460_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364460_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2832128
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21450.049.126191
GTATCAA33500.045.3171461
TCAACGC33550.041.4835784
ATCAACG34650.039.994283
CAACGCA35950.038.5521165
AACGCAG37750.036.7132236
GTACATG92800.036.69571
ACATGGG93800.034.8742683
TACATGG96950.034.7227252
TATCAAC43000.033.8877562
CATGGGG64050.030.737024
ACGCAGA48150.028.6599397
CGCAGAG49550.027.8501728
TAAGGTG18300.024.378195
ATGGGGA36350.022.582185
GTATAGA7450.022.3753031
GAGTACA66600.022.2583141
CATGGGA40300.022.1380374
GTAAGGT17850.021.991694
GGTAAGG17900.021.2653313
Produced by FastQC (version 0.11.5)