##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364460_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2832128 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.92803044212691 31.0 31.0 34.0 28.0 34.0 2 30.984604509400704 31.0 31.0 34.0 27.0 34.0 3 30.925629773795535 31.0 31.0 34.0 27.0 34.0 4 34.54926648795535 37.0 35.0 37.0 31.0 37.0 5 34.700071818787855 37.0 35.0 37.0 32.0 37.0 6 34.79650142931393 37.0 35.0 37.0 32.0 37.0 7 34.69253296461177 37.0 35.0 37.0 32.0 37.0 8 34.538141637666094 37.0 35.0 37.0 31.0 37.0 9 35.70355506530778 39.0 35.0 39.0 30.0 39.0 10-11 35.90771321070234 39.0 36.0 39.0 30.5 39.0 12-13 35.962689362910155 39.0 36.0 39.0 30.5 39.0 14-15 37.14059710578053 40.0 37.0 41.0 31.0 41.0 16-17 37.27313931432478 40.0 37.0 41.0 31.0 41.0 18-19 37.25922645445404 40.0 37.0 41.0 31.0 41.0 20-21 37.20394258310358 40.0 37.0 41.0 31.0 41.0 22-23 37.115736117859086 40.0 37.0 41.0 30.0 41.0 24-25 37.15631496881497 40.0 37.0 41.0 30.5 41.0 26-27 37.15495185952047 40.0 37.0 41.0 30.0 41.0 28-29 37.103116102097076 40.0 37.0 41.0 30.0 41.0 30-31 36.874491195313205 40.0 36.0 41.0 30.0 41.0 32-33 36.94413741186839 40.0 36.0 41.0 30.0 41.0 34-35 36.891428459448164 40.0 36.0 41.0 30.0 41.0 36-37 36.77388398405722 40.0 36.0 41.0 30.0 41.0 38-39 36.6361064895372 40.0 36.0 41.0 30.0 41.0 40-41 36.60564882660671 40.0 36.0 41.0 30.0 41.0 42-43 36.47136587753096 40.0 35.5 41.0 30.0 41.0 44-45 36.455555504553466 40.0 35.0 41.0 30.0 41.0 46-47 36.31202438590346 39.5 35.0 41.0 29.0 41.0 48-49 36.13886748762768 39.0 35.0 41.0 29.0 41.0 50-51 35.99673796523321 39.0 35.0 41.0 28.5 41.0 52-53 35.86160300664377 39.0 35.0 41.0 28.0 41.0 54-55 35.72470188494306 39.0 35.0 40.5 28.0 41.0 56-57 35.6731196471346 38.5 34.5 40.5 28.5 41.0 58-59 35.636242606266386 38.5 35.0 41.0 28.0 41.0 60-61 35.443479072979756 38.0 34.0 40.0 28.0 41.0 62-63 35.17988081753367 37.5 34.0 40.0 28.0 41.0 64-65 34.87038986232261 37.0 34.0 40.0 28.0 41.0 66-67 34.564723593001446 36.5 34.0 40.0 28.0 41.0 68-69 34.2608547353792 36.0 34.0 39.0 27.5 41.0 70-71 33.908416039105575 36.0 33.5 39.0 27.0 41.0 72-73 33.57696474170659 35.0 33.0 38.5 26.0 40.0 74-75 33.221134602673324 35.0 33.0 37.0 26.0 39.5 76-77 32.88143703250701 35.0 33.0 37.0 26.0 39.0 78-79 32.56273657122842 35.0 33.0 36.0 26.0 39.0 80-81 32.258960223549224 35.0 32.5 36.0 26.0 38.0 82-83 31.974820700194343 35.0 32.0 35.5 25.0 37.0 84-85 31.643005718668082 35.0 32.0 35.0 24.5 37.0 86-87 31.467544192917835 35.0 32.0 35.0 24.0 36.0 88-89 31.33787402970487 35.0 32.0 35.0 24.0 36.0 90-91 31.21104536941833 35.0 32.0 35.0 24.0 36.0 92-93 30.985989686906805 35.0 32.0 35.0 23.0 35.5 94-95 30.9093167752305 35.0 32.0 35.0 23.0 35.0 96-97 30.833478748135676 35.0 32.0 35.0 23.0 35.0 98-99 30.68213053929766 34.5 31.5 35.0 20.0 35.0 100-101 30.548285600085872 34.0 31.0 35.0 19.5 35.0 102-103 29.507313052234927 33.5 29.5 34.5 18.5 35.0 104-105 30.47575851091476 34.0 31.5 35.0 20.0 35.0 106-107 30.720736668681642 35.0 32.0 35.0 20.0 35.0 108-109 30.71140834736283 35.0 32.0 35.0 20.0 35.0 110-111 30.59689675042936 35.0 32.0 35.0 19.0 35.0 112-113 30.475812004259694 35.0 32.0 35.0 18.5 35.0 114-115 30.36699894920004 35.0 32.0 35.0 17.0 35.0 116-117 30.238118121779806 35.0 32.0 35.0 9.0 35.0 118-119 29.966900154230316 35.0 31.0 35.0 2.0 35.0 120-121 29.827582651631566 35.0 31.0 35.0 2.0 35.0 122-123 29.585441759694476 35.0 31.0 35.0 2.0 35.0 124-125 28.387280518394647 34.0 28.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6785036028602818 1101 2 0.6256719483979083 1101 3 0.8501234271916687 1101 4 0.7028476379840427 1101 5 0.55630595836206 1101 6 0.588733852924193 1101 7 0.6475705908067084 1101 8 0.7401930656630427 1101 9 1.240559604539932 1101 10-11 0.9945157515974969 1101 12-13 0.9194422380395864 1101 14-15 1.1306074017194305 1101 16-17 0.8530395702988045 1101 18-19 0.9124943400335326 1101 20-21 0.8926739108385604 1101 22-23 0.946884179594683 1101 24-25 0.8801630639955675 1101 26-27 0.8513897907783701 1101 28-29 0.8424582092725075 1101 30-31 1.0428507973990335 1101 32-33 0.8460599106875009 1101 34-35 0.8416175984964198 1101 36-37 0.8546362892905606 1101 38-39 0.9331376748871989 1101 40-41 0.9323060598796289 1101 42-43 0.9261110941355994 1101 44-45 0.8089769040098105 1101 46-47 0.8209686182744775 1101 48-49 0.7555943622074466 1101 50-51 0.6695773590420373 1101 52-53 0.6483881755781482 1101 54-55 0.6277095549103322 1101 56-57 0.6478154394255853 1101 58-59 0.7204202991218978 1101 60-61 0.6656312783065843 1101 62-63 0.76904977871515 1101 64-65 0.6418083918890432 1101 66-67 0.7205141454192798 1101 68-69 0.6353053505086237 1101 70-71 0.6285119433908903 1101 72-73 0.6328374526382703 1101 74-75 0.6356483533947852 1101 76-77 0.6469506311359154 1101 78-79 0.6147786752377584 1101 80-81 0.6830056986849371 1101 82-83 0.68688322094415 1101 84-85 0.8150042345435828 1101 86-87 0.7976978403267481 1101 88-89 0.7538654480785283 1101 90-91 0.7745846129081464 1101 92-93 0.817524957388919 1101 94-95 0.7523210569005734 1101 96-97 0.7614198697140537 1101 98-99 0.7760878656925847 1101 100-101 0.7927885018300564 1101 102-103 0.6752185002765643 1101 104-105 0.6458499564242075 1101 106-107 0.6382002688822546 1101 108-109 0.6767219774979552 1101 110-111 0.6797208844207532 1101 112-113 0.6170789086310968 1101 114-115 0.6701252809294331 1101 116-117 0.6470182668372253 1101 118-119 0.5332792485188804 1101 120-121 0.5198296172262928 1101 122-123 0.4845268315653719 1101 124-125 0.10680453061230821 1102 1 0.44279107122328654 1102 2 0.36147368356865783 1102 3 0.5067429533658476 1102 4 0.42783359867075177 1102 5 0.3581895427962465 1102 6 0.2956422662554985 1102 7 0.37878103535952334 1102 8 0.43950131649449276 1102 9 0.7676229818965865 1102 10-11 0.5812879467676524 1102 12-13 0.537056923228235 1102 14-15 0.7259427612128846 1102 16-17 0.5120975317075036 1102 18-19 0.45955382360639874 1102 20-21 0.6793964830282135 1102 22-23 0.6529402988205248 1102 24-25 0.6294080743151653 1102 26-27 0.5511249384971109 1102 28-29 0.6060632478394439 1102 30-31 0.638282323175801 1102 32-33 0.5242423491870127 1102 34-35 0.4204627300749806 1102 36-37 0.4078253490971022 1102 38-39 0.4890744231278319 1102 40-41 0.37264185722291643 1102 42-43 0.3535097011036541 1102 44-45 0.28783226547425755 1102 46-47 0.35725978117951485 1102 48-49 0.4023298904672359 1102 50-51 0.33930937620147006 1102 52-53 0.31189238817552933 1102 54-55 0.19416219736751117 1102 56-57 0.32848015170200995 1102 58-59 0.44823942938343464 1102 60-61 0.30208491429456785 1102 62-63 0.35482889833974696 1102 64-65 0.4166878626772288 1102 66-67 0.3917241132915521 1102 68-69 0.42980349292737685 1102 70-71 0.5272896059262422 1102 72-73 0.5153160682936928 1102 74-75 0.5588332650093761 1102 76-77 0.5649842060498784 1102 78-79 0.43772390758872604 1102 80-81 0.4543598222528473 1102 82-83 0.44720328782939234 1102 84-85 0.5866493207035894 1102 86-87 0.5205820682796656 1102 88-89 0.4669021522128247 1102 90-91 0.3647055692578647 1102 92-93 0.4719883074837341 1102 94-95 0.49924685568193183 1102 96-97 0.5084670613559688 1102 98-99 0.4692586134677903 1102 100-101 0.4103201160605252 1102 102-103 0.3448493433289599 1102 104-105 0.38354644320823894 1102 106-107 0.31944821855335803 1102 108-109 0.3690897060107119 1102 110-111 0.3313222766493098 1102 112-113 0.309971735579726 1102 114-115 0.24459330677837343 1102 116-117 0.19367883842005185 1102 118-119 0.24069321102768626 1102 120-121 0.08999806488395379 1102 122-123 0.10531912819349998 1102 124-125 -0.07851882349412165 1103 1 0.5310174789618891 1103 2 0.5337899851532519 1103 3 0.782139101932728 1103 4 0.6105037740238544 1103 5 0.4935809806267102 1103 6 0.3698477355829226 1103 7 0.5030372669328287 1103 8 0.5962575683726925 1103 9 1.024959141671772 1103 10-11 0.7785341964529238 1103 12-13 0.6239590454962141 1103 14-15 0.7574990098423058 1103 16-17 0.697794761307172 1103 18-19 0.7486196358429424 1103 20-21 0.703786320832684 1103 22-23 0.7407788830005728 1103 24-25 0.6485706190874652 1103 26-27 0.6977287411984747 1103 28-29 0.6189844729557876 1103 30-31 0.8405390095788263 1103 32-33 0.7589572278043235 1103 34-35 0.693290309000119 1103 36-37 0.6820511233228714 1103 38-39 0.6680223045147358 1103 40-41 0.6593336207439435 1103 42-43 0.7166209800685905 1103 44-45 0.613804260958446 1103 46-47 0.5323866867942328 1103 48-49 0.48004973160171005 1103 50-51 0.5209521236978816 1103 52-53 0.393337365961969 1103 54-55 0.38709690493636373 1103 56-57 0.4531388263606857 1103 58-59 0.5333120803097415 1103 60-61 0.5609244487438616 1103 62-63 0.5040703073372512 1103 64-65 0.6151857062970194 1103 66-67 0.41703416908941193 1103 68-69 0.48299461075264105 1103 70-71 0.5392540642321535 1103 72-73 0.5441778569115812 1103 74-75 0.5629474435137993 1103 76-77 0.5624359413064823 1103 78-79 0.513555799274279 1103 80-81 0.5712555281729479 1103 82-83 0.4795104115999287 1103 84-85 0.5954697685484227 1103 86-87 0.6154774168335564 1103 88-89 0.5732949464104955 1103 90-91 0.5911319883672128 1103 92-93 0.49425384682244555 1103 94-95 0.493377521763815 1103 96-97 0.6226618096970569 1103 98-99 0.5233394919389092 1103 100-101 0.4414792008781454 1103 102-103 0.5095370946020772 1103 104-105 0.4812398067552621 1103 106-107 0.49212148025101143 1103 108-109 0.5755799637204824 1103 110-111 0.5694008647278963 1103 112-113 0.5582900838980756 1103 114-115 0.5617627227282789 1103 116-117 0.5454343999317111 1103 118-119 0.4983477516090531 1103 120-121 0.345423533724059 1103 122-123 0.365991788866161 1103 124-125 0.14539791017871195 1104 1 0.5060926932052929 1104 2 0.5189718203807878 1104 3 0.6563024766445515 1104 4 0.4672834964342911 1104 5 0.5435357029306118 1104 6 0.4772351379429054 1104 7 0.512233113742937 1104 8 0.6512108417903164 1104 9 1.0073392996003747 1104 10-11 0.7015997202040083 1104 12-13 0.767838193465856 1104 14-15 0.7865122650210026 1104 16-17 0.6512425779634015 1104 18-19 0.6664466366909636 1104 20-21 0.8106441872198005 1104 22-23 0.9029135718948567 1104 24-25 0.8592036332396731 1104 26-27 0.8533712476865105 1104 28-29 0.8386093179462648 1104 30-31 0.9102527053894178 1104 32-33 0.8397617751765196 1104 34-35 0.7394823482223174 1104 36-37 0.6753081557480982 1104 38-39 0.8384843074721715 1104 40-41 0.747025704711163 1104 42-43 0.6897823327317738 1104 44-45 0.5638406429189544 1104 46-47 0.6075202293637858 1104 48-49 0.6435366788409453 1104 50-51 0.6635847500599397 1104 52-53 0.5362171020089761 1104 54-55 0.5072794646070449 1104 56-57 0.49004648755993685 1104 58-59 0.502615134050636 1104 60-61 0.6289369283422701 1104 62-63 0.5474333961071025 1104 64-65 0.5007378598107906 1104 66-67 0.56673645492058 1104 68-69 0.4705411136099329 1104 70-71 0.5127899966117866 1104 72-73 0.4885258154734373 1104 74-75 0.47070654912482723 1104 76-77 0.5569658629387888 1104 78-79 0.48807985078644833 1104 80-81 0.573734242328996 1104 82-83 0.5160191496626005 1104 84-85 0.4904866118513702 1104 86-87 0.5266123209300666 1104 88-89 0.5358131640246917 1104 90-91 0.4935641463980929 1104 92-93 0.5259514301653638 1104 94-95 0.5259720296325483 1104 96-97 0.46420931612116334 1104 98-99 0.468001837515331 1104 100-101 0.5181621994761372 1104 102-103 0.5582368789737089 1104 104-105 0.475031488869611 1104 106-107 0.4165071950846233 1104 108-109 0.3629217048556441 1104 110-111 0.42850604961004635 1104 112-113 0.48000851628835406 1104 114-115 0.4520943365745147 1104 116-117 0.3996778256102367 1104 118-119 0.30732184424402575 1104 120-121 0.19709673103253778 1104 122-123 0.30668589062059226 1104 124-125 0.01964848440643152 1105 1 0.25657568925565144 1105 2 0.3118314854219335 1105 3 0.39768830761806484 1105 4 0.23056678027298005 1105 5 0.2761252532847962 1105 6 0.3267201869432128 1105 7 0.407027333252735 1105 8 0.4546878013844946 1105 9 0.8538077014286074 1105 10-11 0.5304464922951482 1105 12-13 0.5606516803923753 1105 14-15 0.14725898578839747 1105 16-17 0.26414192303482764 1105 18-19 0.2531750261492789 1105 20-21 0.5901113065662926 1105 22-23 0.6422485693062328 1105 24-25 0.6170523828046726 1105 26-27 0.6321598980799763 1105 28-29 0.45935430174337455 1105 30-31 0.5705238094252607 1105 32-33 0.45310313991497964 1105 34-35 0.48272955754447366 1105 36-37 0.4665186679778728 1105 38-39 0.5276177785935801 1105 40-41 0.4891225664556771 1105 42-43 0.5627927470058083 1105 44-45 0.47950234699797534 1105 46-47 0.5287792513077463 1105 48-49 0.41774923361721505 1105 50-51 0.39081514024690023 1105 52-53 0.32870833658176934 1105 54-55 0.27391490728661694 1105 56-57 0.29924879390560477 1105 58-59 0.36683268452884477 1105 60-61 0.4216683095100393 1105 62-63 0.42013078855060115 1105 64-65 0.3089914876613804 1105 66-67 0.24505886699805757 1105 68-69 0.21410131926394627 1105 70-71 0.2611117498033053 1105 72-73 0.2875870661654716 1105 74-75 0.23252411767650472 1105 76-77 0.20110027853142043 1105 78-79 0.1644871077931782 1105 80-81 0.17328515442708436 1105 82-83 0.1802886001598658 1105 84-85 0.3482681417886617 1105 86-87 0.3044876850002609 1105 88-89 0.23962676232945768 1105 90-91 0.19014626725205375 1105 92-93 0.23119573406560434 1105 94-95 0.17391890628868723 1105 96-97 0.1254185931947447 1105 98-99 0.1747822840645874 1105 100-101 0.08142246820369081 1105 102-103 0.05489412914679548 1105 104-105 0.02885036030198762 1105 106-107 0.05675061367059442 1105 108-109 0.08257026568958281 1105 110-111 0.162671571555844 1105 112-113 0.15890745358484892 1105 114-115 0.0879155440942192 1105 116-117 0.10022471116031895 1105 118-119 -0.05707491097718531 1105 120-121 -0.18053598247201208 1105 122-123 -0.15228387283730882 1105 124-125 -0.3448967241462739 1106 1 0.3682130789403715 1106 2 0.3986320144362381 1106 3 0.536334148800865 1106 4 0.3188561058357422 1106 5 0.283635440320424 1106 6 0.19792098431491922 1106 7 0.30637295581635726 1106 8 0.4447515570190461 1106 9 0.845050783542483 1106 10-11 0.32928327186030515 1106 12-13 0.4161741610252392 1106 14-15 0.7199758264755189 1106 16-17 0.5287635087446603 1106 18-19 0.4747739468392851 1106 20-21 0.5622492747135226 1106 22-23 0.6628402296754103 1106 24-25 0.581947381245314 1106 26-27 0.6150133611270618 1106 28-29 0.5727035524422917 1106 30-31 0.6074939569393294 1106 32-33 0.6219428641710962 1106 34-35 0.5130762255258929 1106 36-37 0.5593868654804837 1106 38-39 0.65426296653402 1106 40-41 0.6982612290979446 1106 42-43 0.626755094964075 1106 44-45 0.4400790653981588 1106 46-47 0.6191535870834244 1106 48-49 0.4786453327292435 1106 50-51 0.5796172840659324 1106 52-53 0.4590425137531611 1106 54-55 0.34457666470158443 1106 56-57 0.39631728879750483 1106 58-59 0.5592343617776194 1106 60-61 0.4802070557477336 1106 62-63 0.5593802188761643 1106 64-65 0.6452962970282741 1106 66-67 0.5862545185360659 1106 68-69 0.5818685689700942 1106 70-71 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34482.0 3 7016.0 4 4659.0 5 4001.0 6 4490.0 7 5472.0 8 6294.0 9 7014.0 10 7672.0 11 8522.0 12 9170.0 13 9715.0 14 9869.0 15 9660.0 16 9079.0 17 8777.0 18 8502.0 19 8589.0 20 8930.0 21 9496.0 22 11027.0 23 14183.0 24 19630.0 25 26841.0 26 32663.0 27 37404.0 28 44653.0 29 54672.0 30 66396.0 31 81174.0 32 103467.0 33 134964.0 34 185122.0 35 272077.0 36 489877.0 37 785178.0 38 290907.0 39 484.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.765749171059056 18.362482037551324 12.794388011510105 25.077380779879515 2 15.671644689569456 19.852760216304777 39.2318108551383 25.24378423898747 3 18.45502745638615 24.05530399755943 29.71624163879599 27.77342690725843 4 12.737928019734088 16.09213666123598 37.03121480028276 34.13872051874717 5 13.547053981936225 38.06775027656381 34.850358941511836 13.534836799988136 6 33.221791213206814 36.22700216163615 17.14260089812769 13.408605727029348 7 29.021509275537433 32.15957476483223 21.18669113355439 17.632224826075948 8 26.603022559560863 34.92341203692098 20.284996696800928 18.188568706717234 9 26.460347506829695 15.806550364974328 19.975799022427392 37.75730310576858 10-11 24.92499623921821 26.149174852693886 28.073671066840216 20.85215784124769 12-13 25.527842615499864 23.479729759552242 27.787832259119256 23.204595365828638 14-15 22.638626635205053 25.169838869571127 24.90721736850608 27.284317126717745 16-17 23.08022183808955 28.347314001035638 25.853939465736225 22.718524695138584 18-19 23.159860799145964 27.417038067291244 26.835327663900816 22.58777346966198 20-21 23.34300268255576 26.69280710601816 27.083006666129872 22.881183545296206 22-23 23.480471739838606 26.559369601048076 26.77053841868809 23.18962024042523 24-25 23.35705953113907 26.792552705935986 26.635904359051004 23.21448340387394 26-27 22.920336622779335 27.061592052784157 26.58947759835541 23.428593726081097 28-29 23.15720466809115 27.195525841773758 26.56088282560126 23.08638666453383 30-31 23.181569208699862 26.863334240937466 26.470486444031728 23.484610106330944 32-33 22.87869347839098 26.90004606172271 26.49055752339411 23.7307029364922 34-35 23.744227986903677 26.852901167599576 26.165794372888595 23.237076472608152 36-37 23.75382245855167 26.444713227114548 26.247227994811645 23.554236319522136 38-39 23.10845828974115 26.80778822656235 26.4061457826131 23.677607701083392 40-41 23.563039001612946 26.469392900220612 26.571861467581513 23.395706630584925 42-43 23.378975618273557 26.453151849387346 26.357249909474007 23.81062262286509 44-45 23.206966263450898 26.702156151260553 26.62458138491614 23.46629620037241 46-47 23.60973379224019 27.00213711240011 25.933757460252444 23.454371635107258 48-49 23.16308838588933 26.866043998728767 26.217521805148486 23.753345810233412 50-51 23.36491350542735 26.8352013604527 26.39904293734619 23.400842196773763 52-53 23.705679205906968 26.509334689308627 26.356063575466738 23.42892252931767 54-55 23.130027126798183 26.69722580640605 26.55830778498852 23.614439281807247 56-57 23.09200433146017 27.027891234655073 26.4620929258173 23.41801150806746 58-59 23.158122035654337 26.468548957929265 26.491819672793977 23.88150933362242 60-61 22.853118197856652 26.755879947510934 26.704658616271892 23.68634323836052 62-63 23.00147629740162 26.640944025180218 26.950435005393313 23.40714467202484 64-65 22.828734217667737 26.797168670337957 26.50808560375863 23.86601150823568 66-67 22.755077501353398 26.858996022610697 26.49771855424035 23.888207921795548 68-69 23.055581305216467 26.31640653623507 27.000258673736493 23.62775348481197 70-71 23.073504735862326 26.3114722839766 26.639790565563377 23.9752324145977 72-73 22.954872066334705 26.49104682405168 26.471377081501068 24.08270402811255 74-75 22.799728012376814 26.88870631604912 26.510141356226626 23.801424315347436 76-77 23.114723505139604 26.42581855759145 26.65911835135754 23.800339585911402 78-79 23.036062960678798 26.472649316561757 26.766445572036666 23.724842150722775 80-81 23.147132868812136 26.653801753202018 26.534157704840112 23.664907673145738 82-83 23.086853500525937 26.60781823296133 26.678007624245375 23.627320642267364 84-85 22.981285851480777 26.625695311477827 26.734621483758453 23.658397353282943 86-87 23.054306532481416 26.590345054089344 26.64109271689814 23.714255696531094 88-89 22.951314948422663 26.72216395272048 26.40081647932139 23.925704619535466 90-91 22.824373486377127 26.70525132231224 26.687276774836484 23.78309841647415 92-93 23.002957064998224 26.217026112148123 26.882559955252056 23.897456867601598 94-95 22.718420649331296 26.15351965360334 27.11033377614589 24.01772592091947 96-97 22.952524505437484 25.766723525414196 27.121446011939454 24.15930595720886 98-99 22.828100279295136 26.0768152550689 26.85551883763505 24.239565628000914 100-101 22.6321911613951 25.919485900798318 27.23420251307725 24.214120424729334 102-103 22.423441240725186 25.679308410511986 27.415980426552693 24.48126992221013 104-105 22.238401400649547 26.031285787383347 27.109228699896782 24.621084112070328 106-107 22.561597210721658 25.875683751373153 26.80900371364699 24.753715324258195 108-109 22.498980415143173 25.81482926588125 26.86075560506844 24.82543471390714 110-111 22.473188864590657 25.90762018532179 27.137166443203753 24.4820245068838 112-113 22.438313494624328 26.078493099724053 27.367654591235258 24.11553881441636 114-115 22.33605486379399 26.30564599857331 27.006451863518542 24.35184727411415 116-117 22.27287101308509 26.10771448160108 27.27285247370896 24.34656203160487 118-119 22.09709067682503 26.291024323095353 26.96735393668953 24.644531063390087 120-121 22.170745757120795 26.39312267362579 27.149076196117033 24.287055373136386 122-123 22.054410755003264 26.47177037683764 27.184768972561518 24.289049895597582 124-125 21.989164023680868 26.42766359347675 27.169717027977956 24.41345535486442 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 334.0 1 305.0 2 268.0 3 235.0 4 204.5 5 173.5 6 150.0 7 130.0 8 120.0 9 127.5 10 123.0 11 118.0 12 119.0 13 143.0 14 184.0 15 214.0 16 212.5 17 229.0 18 295.5 19 382.5 20 524.0 21 756.0 22 1135.5 23 1645.5 24 2652.0 25 4365.5 26 6780.0 27 10537.5 28 14537.5 29 19003.5 30 24289.5 31 30553.0 32 40998.5 33 46990.0 34 48195.5 35 53606.5 36 63757.0 37 73741.0 38 79991.0 39 84247.5 40 86595.5 41 89141.5 42 91685.0 43 94794.0 44 98349.0 45 103671.5 46 106530.0 47 107675.0 48 107575.0 49 106051.0 50 106524.0 51 104409.0 52 103301.5 53 105395.5 54 109176.5 55 105714.5 56 96038.5 57 87365.5 58 80505.0 59 71004.0 60 56235.0 61 46207.0 62 39741.0 63 30049.0 64 21896.0 65 17385.0 66 12427.5 67 9256.0 68 7151.0 69 5048.5 70 3656.5 71 2460.5 72 1865.5 73 1476.0 74 1181.0 75 923.5 76 597.0 77 311.0 78 185.5 79 115.0 80 57.0 81 39.0 82 26.5 83 16.5 84 18.5 85 15.5 86 8.5 87 8.5 88 11.5 89 9.5 90 7.5 91 7.0 92 4.5 93 4.5 94 6.0 95 3.5 96 1.0 97 1.5 98 1.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09229808822200126 2 4.5901880141010576E-4 3 0.0 4 0.002612876254180602 5 0.0018007660670704148 6 4.94327939980114E-4 7 3.530913857000813E-5 8 8.827284642502033E-4 9 3.884005242700895E-4 10-11 0.009533467413902198 12-13 0.002030275467775468 14-15 0.0037251141191358586 16-17 0.0022068211606255084 18-19 0.013858836888728193 20-21 0.017654569285004067 22-23 0.0095511219831872 24-25 0.010857560110277501 26-27 0.010363232170297387 28-29 0.007803319623971797 30-31 0.017548641869294043 32-33 0.002065584606345476 34-35 0.0012534744192352887 36-37 6.179099249751424E-4 38-39 2.47163969990057E-4 40-41 0.0014123655428003256 42-43 0.0024186759920455576 44-45 7.591464792551749E-4 46-47 0.0012534744192352887 48-49 0.008050483593961856 50-51 0.004572533444816053 52-53 0.007697392208261773 54-55 0.008544811533941968 56-57 0.010186686477447348 58-59 0.008562466103226973 60-61 0.01059274157100244 62-63 0.013470436364458103 64-65 0.008580120672511977 66-67 0.013099690409473019 68-69 0.013258581533038055 70-71 0.004237096628400976 72-73 0.01297610842447799 74-75 0.013470436364458103 76-77 0.005278716216216216 78-79 0.0082446838560969 80-81 0.013947109735153214 82-83 0.017778151269999095 84-85 0.013894146027298201 86-87 0.01661294969718883 88-89 0.016171585465063726 90-91 0.012216961945222813 92-93 0.009392230859622165 94-95 0.008121101871101872 96-97 0.009056794043207087 98-99 0.006267372096176444 100-101 0.01062805070957245 102-103 0.011740288574527705 104-105 0.010557432432432432 106-107 0.004978588538371147 108-109 0.003283749887010757 110-111 0.006673427189731538 112-113 0.008774320934647022 114-115 0.013699945765163158 116-117 0.011210651495977584 118-119 0.21937567793546053 120-121 0.026022835126096 122-123 0.027399891530326317 124-125 0.23745395688330473 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2832128.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.59940251231453 #Duplication Level Percentage of deduplicated Percentage of total 1 83.2529821105811 53.7809290171195 2 9.318708505657701 12.039660033038217 3 3.0895024747334507 5.987400417842941 4 1.4496937911852943 3.7459741094552834 5 0.7970064065776881 2.5743068831702742 6 0.48704606490429825 1.8877730873274978 7 0.3355794741464588 1.5174763467680557 8 0.21965522209348404 1.1351676884759057 9 0.1622690182303166 0.9434233461544493 >10 0.7893134928174448 9.247405821809377 >50 0.06042075786272265 2.690872190685991 >100 0.03653813880679267 3.9394643283584543 >500 0.0012845424032852633 0.510146729794071 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.061827714001627E-5 2 0.0 0.0 0.0 0.0 7.061827714001627E-5 3 0.0 0.0 0.0 0.0 7.061827714001627E-5 4 0.0 0.0 0.0 0.0 1.059274157100244E-4 5 0.0 0.0 0.0 0.0 1.4123655428003253E-4 6 0.0 0.0 0.0 0.0 2.118548314200488E-4 7 0.0 0.0 0.0 0.0 2.118548314200488E-4 8 0.0 0.0 0.0 0.0 2.8247310856006506E-4 9 0.0 0.0 0.0 0.0 2.8247310856006506E-4 10-11 0.0 0.0 0.0 0.0 2.8247310856006506E-4 12-13 0.0 0.0 0.0 0.0 6.355644942601464E-4 14-15 0.0 0.0 0.0 5.2963707855012196E-5 7.414919099701708E-4 16-17 0.0 0.0 0.0 7.061827714001627E-5 7.76801048540179E-4 18-19 0.0 0.0 0.0 7.061827714001627E-5 7.76801048540179E-4 20-21 0.0 0.0 0.0 8.827284642502034E-5 9.003830335352074E-4 22-23 0.0 0.0 0.0 1.7654569285004067E-4 9.533467413902196E-4 24-25 0.0 0.0 0.0 3.5309138570008134E-4 9.710013106752236E-4 26-27 0.0 0.0 0.0 9.003830335352074E-4 0.0010239650185302359 28-29 0.0 0.0 0.0 0.003672150411280846 0.0010239650185302359 30-31 0.0 0.0 0.0 0.012976108424477991 0.001076928726385248 32-33 0.0 0.0 0.0 0.033367135948657686 0.0011298924342402602 34-35 0.0 0.0 0.0 0.06353879485672964 0.0011828561420952725 36-37 0.0 0.0 0.0 0.11445457267468137 0.0012005107113802766 38-39 0.0 0.0 0.0 0.1943768078278948 0.001253474419235289 40-41 0.0 3.530913857000813E-5 0.0 0.27911874039591433 0.0012711289885202928 42-43 0.0 3.530913857000813E-5 0.0 0.3701633541986803 0.001306438127090301 44-45 0.0 3.530913857000813E-5 0.0 0.48385878039410646 0.0013770564042303174 46-47 0.0 3.530913857000813E-5 0.0 0.6153323578595318 0.0015182929585103497 48-49 0.0 3.530913857000813E-5 0.0 0.7568513852481243 0.0016771840820753864 50-51 0.0 3.530913857000813E-5 0.0 0.907091769863509 0.0017654569285004069 52-53 0.0 3.530913857000813E-5 0.0 1.0527949301726476 0.0019596571906354515 54-55 0.0 3.530913857000813E-5 0.0 1.2162056234746452 0.0020479300370604718 56-57 0.0 3.530913857000813E-5 0.0 1.4080048641869294 0.00208323917563048 58-59 0.0 3.530913857000813E-5 0.0 1.6094081905902557 0.002118548314200488 60-61 0.0 3.530913857000813E-5 0.0 1.8283071951550212 0.0021362028834854925 62-63 0.0 3.530913857000813E-5 0.0 2.044540359757751 0.002365712284190545 64-65 0.0 3.530913857000813E-5 0.0 2.2769098006869744 0.0023833668534755494 66-67 0.0 3.530913857000813E-5 0.0 2.552815409473018 0.0024363305613305615 68-69 0.0 3.530913857000813E-5 0.0 2.8576745118864686 0.0024716396999005697 70-71 0.0 3.530913857000813E-5 0.0 3.16843024044111 0.0025422579770405856 72-73 0.0 3.530913857000813E-5 0.0 3.490343656783874 0.0028247310856006507 74-75 0.0 3.530913857000813E-5 0.0 3.8315535173551476 0.0030542404863057038 76-77 0.0 3.530913857000813E-5 0.0 4.2084256078821305 0.0030895496248757115 78-79 0.0 3.530913857000813E-5 0.0 4.625567770948206 0.003160167902015728 80-81 0.0 3.530913857000813E-5 0.0 5.059976102775016 0.0032660953177257525 82-83 0.0 3.530913857000813E-5 0.0 5.500704770405857 0.00351325928771581 84-85 0.0 3.530913857000813E-5 0.0 5.963166212826539 0.0035309138570008137 86-87 0.0 3.530913857000813E-5 0.0 6.458677008948748 0.003566222995570822 88-89 0.0 3.530913857000813E-5 0.0 6.98676048540179 0.0036015321341408297 90-91 0.0 3.530913857000813E-5 0.0 7.553807596266836 0.0036015321341408297 92-93 0.0 3.530913857000813E-5 0.0 8.11527586323782 0.0038133869655608784 94-95 0.0 3.530913857000813E-5 0.0 8.69860048698364 0.0038133869655608784 96-97 0.0 3.530913857000813E-5 0.0 9.318011050348007 0.004184132920545964 98-99 0.0 3.530913857000813E-5 0.0 9.971018259061736 0.004378333182681009 100-101 0.0 3.530913857000813E-5 0.0 10.662477119678206 0.004378333182681009 102-103 0.0 3.530913857000813E-5 0.0 11.37667506553376 0.004466606029106029 104-105 0.0 3.530913857000813E-5 0.0 12.09117313906716 0.004519569736961041 106-107 0.0 3.530913857000813E-5 0.0 12.83118206521739 0.004554878875531049 108-109 0.0 3.530913857000813E-5 0.0 13.607541749525446 0.004590188014101057 110-111 0.0 3.530913857000813E-5 0.0 14.416068059070776 0.004625497152671066 112-113 0.0 3.530913857000813E-5 0.0 15.240289280710478 0.004660806291241074 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2145 0.0 49.12619 1 GTATCAA 3350 0.0 45.317146 1 TCAACGC 3355 0.0 41.483578 4 ATCAACG 3465 0.0 39.99428 3 CAACGCA 3595 0.0 38.552116 5 AACGCAG 3775 0.0 36.713223 6 GTACATG 9280 0.0 36.6957 1 ACATGGG 9380 0.0 34.874268 3 TACATGG 9695 0.0 34.722725 2 TATCAAC 4300 0.0 33.887756 2 CATGGGG 6405 0.0 30.73702 4 ACGCAGA 4815 0.0 28.659939 7 CGCAGAG 4955 0.0 27.850172 8 TAAGGTG 1830 0.0 24.37819 5 ATGGGGA 3635 0.0 22.58218 5 GTATAGA 745 0.0 22.375303 1 GAGTACA 6660 0.0 22.258314 1 CATGGGA 4030 0.0 22.138037 4 GTAAGGT 1785 0.0 21.99169 4 GGTAAGG 1790 0.0 21.265331 3 >>END_MODULE