FastQCFastQC Report
Thu 26 May 2016
SRR1364460_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364460_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2832128
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31000.066.298991
GTATCAA54600.051.8263321
TCAACGC65800.041.6283844
ATCAACG66200.041.467543
CAACGCA67150.040.84845
TATCAAC71100.038.6962052
AACGCAG70950.038.6578756
GTACATG93750.032.7254371
ACATGGG90050.032.3363083
TACATGG95600.031.146422
ACGCAGA87850.031.085687
CGCAGAG90750.030.0923088
TAAGGTG16850.027.8963475
CATGGGG62450.027.0796814
GTAAGGT17500.026.5408124
GCAGAGT110500.024.3907629
GGTAAGG18200.024.2117213
CAGAGTA98250.023.52599110-11
GAGTACA69100.023.0188051
GAGTACT60350.022.92108312-13
Produced by FastQC (version 0.11.5)