FastQCFastQC Report
Thu 26 May 2016
SRR1364459_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364459_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2749634
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31650.044.2021181
ATCAACG33100.040.073243
GTACATG97150.039.5244521
GGTATCA22850.038.298351
CAACGCA34500.038.2774545
TCAACGC35500.037.0303044
TACATGG102650.036.8532452
AACGCAG35750.036.4372526
ACATGGG100500.036.3395773
TATCAAC39100.034.532392
CATGGGG70050.031.4186084
ACGCAGA45650.028.2746057
CGCAGAG47400.027.3561958
CATGGGA39250.026.0664654
ATGGGGA38400.023.2364185
AGTACAT67550.022.3658752
CGTATAG1602.6031205E-422.324421
TAAGGTG11550.021.6309935
GAGTACA74400.021.364231
TATTCCG4052.0008883E-1120.5627965
Produced by FastQC (version 0.11.5)