##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364459_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2749634 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.839913966731572 31.0 31.0 34.0 27.0 34.0 2 30.872293912571635 31.0 31.0 34.0 27.0 34.0 3 30.81272634830672 31.0 31.0 34.0 27.0 34.0 4 34.429325502957845 37.0 35.0 37.0 30.0 37.0 5 34.570538842624146 37.0 35.0 37.0 32.0 37.0 6 34.71113355450216 37.0 35.0 37.0 32.0 37.0 7 34.57110873665368 37.0 35.0 37.0 31.0 37.0 8 34.43615586656261 37.0 35.0 37.0 30.0 37.0 9 35.609337824597745 39.0 35.0 39.0 30.0 39.0 10-11 35.80807500198208 39.0 35.0 39.0 30.5 39.0 12-13 35.86563175317151 39.0 35.0 39.0 30.0 39.0 14-15 37.02703141581752 40.0 37.0 41.0 30.5 41.0 16-17 37.160198048176596 40.0 37.0 41.0 31.0 41.0 18-19 37.125578713385124 40.0 37.0 41.0 31.0 41.0 20-21 37.07289224674993 40.0 37.0 41.0 30.5 41.0 22-23 36.96172236013957 40.0 37.0 41.0 30.0 41.0 24-25 37.00213828458624 40.0 37.0 41.0 30.0 41.0 26-27 37.003892154374 40.0 37.0 41.0 30.0 41.0 28-29 36.94572041224396 40.0 36.5 41.0 30.0 41.0 30-31 36.72195554026463 40.0 36.0 41.0 30.0 41.0 32-33 36.80598436010029 40.0 36.0 41.0 30.0 41.0 34-35 36.754554424334295 40.0 36.0 41.0 30.0 41.0 36-37 36.63376034774083 40.0 36.0 41.0 30.0 41.0 38-39 36.49150632411441 40.0 36.0 41.0 30.0 41.0 40-41 36.46039236494748 40.0 36.0 41.0 30.0 41.0 42-43 36.320814333834974 40.0 35.0 41.0 29.0 41.0 44-45 36.29078597369686 39.5 35.0 41.0 29.0 41.0 46-47 36.13500578622464 39.0 35.0 41.0 29.0 41.0 48-49 35.959063824494464 39.0 35.0 41.0 28.0 41.0 50-51 35.810464774584545 39.0 35.0 41.0 28.0 41.0 52-53 35.67936769039079 39.0 35.0 40.5 28.0 41.0 54-55 35.54635307826423 38.5 34.5 40.0 28.0 41.0 56-57 35.48014444831567 38.5 34.5 40.0 28.0 41.0 58-59 35.44591734754516 38.0 34.0 40.0 28.0 41.0 60-61 35.24263792926622 38.0 34.0 40.0 28.0 41.0 62-63 34.969908176870085 37.5 34.0 40.0 28.0 41.0 64-65 34.65120175994332 37.0 34.0 40.0 27.5 41.0 66-67 34.33354184593295 36.5 34.0 39.5 27.0 41.0 68-69 34.02686393898243 36.0 33.5 39.0 26.5 41.0 70-71 33.68489406226429 35.5 33.0 39.0 26.0 40.5 72-73 33.35394128818599 35.0 33.0 37.5 26.0 40.0 74-75 32.99942774201948 35.0 33.0 37.0 26.0 39.0 76-77 32.66762340005979 35.0 33.0 37.0 26.0 39.0 78-79 32.37228427492532 35.0 32.5 36.0 26.0 39.0 80-81 32.07412841127219 35.0 32.0 36.0 25.0 37.0 82-83 31.812210097780287 35.0 32.0 35.0 25.0 37.0 84-85 31.49286650514214 35.0 32.0 35.0 24.5 36.5 86-87 31.323114639984812 35.0 32.0 35.0 24.0 36.0 88-89 31.204041519707715 35.0 32.0 35.0 24.0 36.0 90-91 31.10042172885555 35.0 32.0 35.0 24.0 36.0 92-93 30.89329925364612 35.0 32.0 35.0 23.0 35.0 94-95 30.82961641440279 34.5 31.5 35.0 23.0 35.0 96-97 30.762732785527092 34.0 31.5 35.0 22.5 35.0 98-99 30.620181449603837 34.0 31.0 35.0 20.0 35.0 100-101 30.49928044968894 34.0 31.0 35.0 19.5 35.0 102-103 29.419517652167528 33.5 29.5 34.5 18.5 35.0 104-105 30.42700464861869 34.0 31.0 35.0 20.0 35.0 106-107 30.694313497723698 35.0 32.0 35.0 20.0 35.0 108-109 30.68889641312262 35.0 32.0 35.0 20.0 35.0 110-111 30.58623638636997 35.0 32.0 35.0 19.0 35.0 112-113 30.481151491434858 35.0 32.0 35.0 18.5 35.0 114-115 30.38833877526973 35.0 32.0 35.0 17.5 35.0 116-117 30.273288190355515 35.0 32.0 35.0 12.5 35.0 118-119 30.012785156133507 35.0 31.0 35.0 2.0 35.0 120-121 29.893192148482306 35.0 31.0 35.0 2.0 35.0 122-123 29.664297866552424 35.0 31.0 35.0 2.0 35.0 124-125 28.460555841250144 34.0 28.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6444889821242548 1101 2 0.6265122514063428 1101 3 0.7470840499226981 1101 4 0.6515687891497279 1101 5 0.587599505768388 1101 6 0.6103177094018406 1101 7 0.7069559125617673 1101 8 0.7732435779836564 1101 9 1.2668714994384729 1101 10-11 1.0150890669971773 1101 12-13 0.875744170503026 1101 14-15 1.0023368000336959 1101 16-17 0.7459735543869002 1101 18-19 0.7972535393401756 1101 20-21 0.9901396909110218 1101 22-23 0.9762472607494885 1101 24-25 0.9522294308699841 1101 26-27 0.8731927597018441 1101 28-29 0.8770796471352043 1101 30-31 1.0463035271919736 1101 32-33 0.9093162162996009 1101 34-35 0.8595141851644073 1101 36-37 0.8746144059866268 1101 38-39 0.9614217715787774 1101 40-41 0.8832685648629734 1101 42-43 0.8995598676200274 1101 44-45 0.7348457554286654 1101 46-47 0.7103671624767074 1101 48-49 0.6985895670627329 1101 50-51 0.7357992225553858 1101 52-53 0.624412973404624 1101 54-55 0.6180791734639541 1101 56-57 0.648167781570244 1101 58-59 0.7716724854717185 1101 60-61 0.7143599088091364 1101 62-63 0.6420546711525503 1101 64-65 0.5428649511089958 1101 66-67 0.6315506075757185 1101 68-69 0.6114630582243024 1101 70-71 0.5531056425694274 1101 72-73 0.5854879684738492 1101 74-75 0.6192800186420229 1101 76-77 0.726051043658714 1101 78-79 0.6114975733845895 1101 80-81 0.606143852181539 1101 82-83 0.6178145528261183 1101 84-85 0.7046393567157061 1101 86-87 0.6461072387066302 1101 88-89 0.6030877223863484 1101 90-91 0.6209071375532957 1101 92-93 0.763333824672749 1101 94-95 0.6473578972478258 1101 96-97 0.6168398914729565 1101 98-99 0.6569460759149628 1101 100-101 0.6817814325039784 1101 102-103 0.6198001168494791 1101 104-105 0.5442952153814069 1101 106-107 0.41314786878207244 1101 108-109 0.3963308698749053 1101 110-111 0.45173087865814665 1101 112-113 0.3759732696083695 1101 114-115 0.4303497670199299 1101 116-117 0.419099895584651 1101 118-119 0.2839004695268841 1101 120-121 0.2778521678738777 1101 122-123 0.3273637208873055 1101 124-125 -0.017000506132919924 1102 1 0.36802726646859085 1102 2 0.2936062055814901 1102 3 0.42743061452875963 1102 4 0.40254706357440284 1102 5 0.351829560824406 1102 6 0.33001366609673255 1102 7 0.35519699826204487 1102 8 0.46126258798060604 1102 9 0.7131348052746205 1102 10-11 0.47436016275462833 1102 12-13 0.46067261570045304 1102 14-15 0.6408933541937643 1102 16-17 0.47778849675376733 1102 18-19 0.3962302245801297 1102 20-21 0.6829025761573178 1102 22-23 0.6409960531540264 1102 24-25 0.5250194197411915 1102 26-27 0.5035052551083155 1102 28-29 0.4820658890582834 1102 30-31 0.5918871738339178 1102 32-33 0.4410202500264475 1102 34-35 0.2588225709292189 1102 36-37 0.2636694622399247 1102 38-39 0.38568575713932063 1102 40-41 0.3742279577910921 1102 42-43 0.3625344181413155 1102 44-45 0.3240192774252222 1102 46-47 0.4153994642032117 1102 48-49 0.4294769713216482 1102 50-51 0.28967496741904597 1102 52-53 0.2904721378770674 1102 54-55 0.14427600524176398 1102 56-57 0.1839191923480641 1102 58-59 0.326598993679859 1102 60-61 0.29065586014589684 1102 62-63 0.26940478208489793 1102 64-65 0.28270592239176295 1102 66-67 0.2517255601467525 1102 68-69 0.2444903318395646 1102 70-71 0.40622219787699265 1102 72-73 0.31919206811049605 1102 74-75 0.30134080098653726 1102 76-77 0.288568261999842 1102 78-79 0.22422913488245655 1102 80-81 0.35487464409957425 1102 82-83 0.3540868821398959 1102 84-85 0.43145297851582143 1102 86-87 0.3917957779416916 1102 88-89 0.3128006864161996 1102 90-91 0.27166162688752493 1102 92-93 0.2603413006927262 1102 94-95 0.30741586230425355 1102 96-97 0.2669421879788345 1102 98-99 0.20433667039760195 1102 100-101 0.1596525153165551 1102 102-103 0.10520601686669195 1102 104-105 0.16007714974378828 1102 106-107 0.20176265116357683 1102 108-109 0.2302212358070328 1102 110-111 0.2526299250050599 1102 112-113 0.177438357806583 1102 114-115 0.045430046572288774 1102 116-117 0.11611105828558976 1102 118-119 0.21777540118696237 1102 120-121 0.09250531995053635 1102 122-123 0.09340952558662607 1102 124-125 -0.08851242584815466 1103 1 0.3499605153634846 1103 2 0.41333059035716957 1103 3 0.5529317630753141 1103 4 0.5014648415412424 1103 5 0.29249080489743307 1103 6 0.32180668087266184 1103 7 0.4210150066571572 1103 8 0.4569300451093241 1103 9 0.900935826923849 1103 10-11 0.5982948064415723 1103 12-13 0.5438934709572294 1103 14-15 0.7162472946610734 1103 16-17 0.6363460373183685 1103 18-19 0.6475069266398421 1103 20-21 0.6694422336663735 1103 22-23 0.6219924906825582 1103 24-25 0.5546909246427347 1103 26-27 0.6174065028720364 1103 28-29 0.5503790354527425 1103 30-31 0.9188984484030485 1103 32-33 0.8343073339404512 1103 34-35 0.6826493698694662 1103 36-37 0.5934801767353335 1103 38-39 0.6763384934601149 1103 40-41 0.5701546017943855 1103 42-43 0.6375546390313218 1103 44-45 0.44114190768669914 1103 46-47 0.4098547463789899 1103 48-49 0.510118869245396 1103 50-51 0.4402939363513738 1103 52-53 0.45215942286160526 1103 54-55 0.2694835464654233 1103 56-57 0.36699754275481666 1103 58-59 0.36309308678342944 1103 60-61 0.4063649453468301 1103 62-63 0.4100491304809495 1103 64-65 0.46885389377217024 1103 66-67 0.2829677039565155 1103 68-69 0.3297070638321955 1103 70-71 0.4085372051924594 1103 72-73 0.3260990747836914 1103 74-75 0.36154312372272557 1103 76-77 0.3168897304139122 1103 78-79 0.265655357692097 1103 80-81 0.3463472101934215 1103 82-83 0.25525705755536876 1103 84-85 0.3397042699566626 1103 86-87 0.366757283358492 1103 88-89 0.3358210163492288 1103 90-91 0.2963971076893621 1103 92-93 0.2993401479522113 1103 94-95 0.27876447883589606 1103 96-97 0.3107841630714958 1103 98-99 0.2741100346624066 1103 100-101 0.18738548093924123 1103 102-103 0.18990353631768997 1103 104-105 0.1017160663594261 1103 106-107 0.19068846356404734 1103 108-109 0.1545847421033706 1103 110-111 0.23662450640567556 1103 112-113 0.21016711149277612 1103 114-115 0.20045246629685565 1103 116-117 0.13054721599295505 1103 118-119 0.1026712950581441 1103 120-121 0.020384864558121052 1103 122-123 0.14122968368702615 1103 124-125 -0.05027418362166358 1104 1 0.45525482131690964 1104 2 0.4418982079663927 1104 3 0.5295766036774197 1104 4 0.40041612337304144 1104 5 0.36757455812063 1104 6 0.3267456878883479 1104 7 0.43991585480145545 1104 8 0.5781633780127038 1104 9 0.9279413018107832 1104 10-11 0.717716232022859 1104 12-13 0.6326442727250452 1104 14-15 0.751810783532477 1104 16-17 0.6391673219526837 1104 18-19 0.5502761476674394 1104 20-21 0.639712155324716 1104 22-23 0.6691564898711135 1104 24-25 0.6602324740978744 1104 26-27 0.6589350270446772 1104 28-29 0.6430213866170718 1104 30-31 0.6355256178665698 1104 32-33 0.6520993093439174 1104 34-35 0.6121753565275796 1104 36-37 0.46487582731348454 1104 38-39 0.6333018249680933 1104 40-41 0.5988262293566748 1104 42-43 0.5503053563716662 1104 44-45 0.5292640179513199 1104 46-47 0.5190561094135973 1104 48-49 0.4559526960494793 1104 50-51 0.5611594820870209 1104 52-53 0.3624996479780691 1104 54-55 0.36811936857205296 1104 56-57 0.3599705095478498 1104 58-59 0.43520085962194344 1104 60-61 0.4930115096764638 1104 62-63 0.4990282381998483 1104 64-65 0.35196362134113457 1104 66-67 0.3825726537514882 1104 68-69 0.4086333516149452 1104 70-71 0.4277861407614054 1104 72-73 0.37257888899635105 1104 74-75 0.4017070595906844 1104 76-77 0.4016148076005521 1104 78-79 0.21151207958449447 1104 80-81 0.32148821579495035 1104 82-83 0.31559426321717154 1104 84-85 0.29467871892084574 1104 86-87 0.3168652801643219 1104 88-89 0.3478290944164968 1104 90-91 0.2881943633275377 1104 92-93 0.2634419686540639 1104 94-95 0.2762501525495189 1104 96-97 0.29498939569849014 1104 98-99 0.2912782141833503 1104 100-101 0.217154577670847 1104 102-103 0.14268087762629378 1104 104-105 0.1613426741457502 1104 106-107 0.18905329694291595 1104 108-109 0.11053019492189975 1104 110-111 0.22238446503621745 1104 112-113 0.2601446854459617 1104 114-115 0.2211552839464197 1104 116-117 0.18403852641282725 1104 118-119 0.1146722202425039 1104 120-121 0.03951753646476064 1104 122-123 0.1301585453350249 1104 124-125 -0.1503732979540473 1105 1 0.2930073209609212 1105 2 0.3403168971497479 1105 3 0.3922506887156416 1105 4 0.1965430992520396 1105 5 0.17004266525722045 1105 6 0.20798458661877817 1105 7 0.30403617460240895 1105 8 0.4340350512043756 1105 9 0.8215101592330001 1105 10-11 0.3519584758392753 1105 12-13 0.45805792450382654 1105 14-15 0.11043219312921337 1105 16-17 0.2472527509602287 1105 18-19 0.2807404027538638 1105 20-21 0.6107941832107286 1105 22-23 0.6834373970911543 1105 24-25 0.6174026361131908 1105 26-27 0.5300034736239283 1105 28-29 0.4463135988966158 1105 30-31 0.5542916813203007 1105 32-33 0.46232904328025626 1105 34-35 0.389385890018076 1105 36-37 0.4462954575677571 1105 38-39 0.5769282060319867 1105 40-41 0.5197923913677016 1105 42-43 0.49928718313169185 1105 44-45 0.3758535655998543 1105 46-47 0.48727737061739873 1105 48-49 0.3697115949982006 1105 50-51 0.3609761400717417 1105 52-53 0.17953305962426924 1105 54-55 0.1754714576487686 1105 56-57 0.16031297901987074 1105 58-59 0.3425090035507097 1105 60-61 0.2638209381189327 1105 62-63 0.25751575321509534 1105 64-65 0.3023865631500726 1105 66-67 0.17792539950183084 1105 68-69 0.18735327238203325 1105 70-71 0.21185211170584495 1105 72-73 0.1431983816822182 1105 74-75 0.22906116729742365 1105 76-77 0.27644615944523565 1105 78-79 0.21818663923706794 1105 80-81 0.1122585926357047 1105 82-83 0.16903764489279638 1105 84-85 0.27815157945854807 1105 86-87 0.28826374574670766 1105 88-89 0.23733526201196042 1105 90-91 0.13704538983859393 1105 92-93 0.053069861665093754 1105 94-95 0.05848270334116279 1105 96-97 0.06055325537985112 1105 98-99 0.03770669338473809 1105 100-101 -0.07364421076857042 1105 102-103 -0.12316965789858259 1105 104-105 -0.09393956245580526 1105 106-107 -0.14552073245920738 1105 108-109 -0.03581423658198446 1105 110-111 0.0028666925685634226 1105 112-113 -0.02914817574323436 1105 114-115 -0.1644583258507133 1105 116-117 -0.17751903905860544 1105 118-119 -0.3704264307354066 1105 120-121 -0.3316975252156915 1105 122-123 -0.3148445886612201 1105 124-125 -0.4921215091907385 1106 1 0.4684560317233597 1106 2 0.440204555021527 1106 3 0.5661817124175634 1106 4 0.4080863531684855 1106 5 0.37808459958071694 1106 6 0.3764724026912134 1106 7 0.23306399527662336 1106 8 0.5442931403951192 1106 9 0.9623697102610578 1106 10-11 0.4290405114085516 1106 12-13 0.5802867422343354 1106 14-15 0.7357318678022722 1106 16-17 0.6530069990256848 1106 18-19 0.6089376617553341 1106 20-21 0.7599081590317667 1106 22-23 0.7238710059583013 1106 24-25 0.600338365023994 1106 26-27 0.7048955270263448 1106 28-29 0.691912809002865 1106 30-31 0.7640911138478259 1106 32-33 0.78235234549728 1106 34-35 0.7169823303770286 1106 36-37 0.729206282591143 1106 38-39 0.8073996502426937 1106 40-41 0.7340032284255926 1106 42-43 0.6947820402372571 1106 44-45 0.4876719377150067 1106 46-47 0.584231598821269 1106 48-49 0.6147924043778374 1106 50-51 0.5261333495686316 1106 52-53 0.5417356321507967 1106 54-55 0.44864057289559867 1106 56-57 0.4223564171339049 1106 58-59 0.48088772312947725 1106 60-61 0.4836696439818766 1106 62-63 0.5269265181209164 1106 64-65 0.519384998775088 1106 66-67 0.4210266134812102 1106 68-69 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-1.2705526760422003 2216 74-75 -1.178088470781887 2216 76-77 -1.1032362877955748 2216 78-79 -1.1058725758835877 2216 80-81 -0.9931260109370612 2216 82-83 -1.200910625770181 2216 84-85 -1.3072507241475293 2216 86-87 -1.2782541945909784 2216 88-89 -1.7758470183855266 2216 90-91 -1.4270361176516495 2216 92-93 -1.331078890239219 2216 94-95 -1.286514086053593 2216 96-97 -1.1875813056849864 2216 98-99 -1.1681217220965046 2216 100-101 -1.2234073269212615 2216 102-103 -1.5021531538010642 2216 104-105 -1.3595568855522693 2216 106-107 -1.2095418131294977 2216 108-109 -1.160382684762581 2216 110-111 -1.1277186242090274 2216 112-113 -1.293097830841031 2216 114-115 -1.2610482636348088 2216 116-117 -1.1770451897405962 2216 118-119 -0.9727040907742719 2216 120-121 -0.9641943853932347 2216 122-123 -0.881699633622187 2216 124-125 -0.7351970011897819 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38053.0 3 7578.0 4 5130.0 5 4560.0 6 4743.0 7 5598.0 8 6494.0 9 7074.0 10 7836.0 11 8426.0 12 9003.0 13 9296.0 14 9312.0 15 9151.0 16 8661.0 17 8183.0 18 7857.0 19 7774.0 20 7934.0 21 8618.0 22 10143.0 23 13309.0 24 18634.0 25 25882.0 26 31276.0 27 37011.0 28 44701.0 29 54114.0 30 65435.0 31 79833.0 32 101141.0 33 133521.0 34 184795.0 35 272432.0 36 492382.0 37 756030.0 38 247382.0 39 332.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.466879318698126 17.9535172216477 12.518514231342259 25.06108922831191 2 16.133903301758128 19.39085533814417 38.832590323193045 25.642651036904656 3 18.81971157605397 23.530740284248843 29.04987683810894 28.59967130158825 4 12.878963939439236 15.539308160660816 36.21829457634997 35.363433323549984 5 14.092886237998254 37.6069973814373 34.24469013674717 14.055426243817282 6 34.28183739764069 35.16875737372474 16.982151767439085 13.567253461195477 7 30.04290752878383 31.474443507754124 20.581793795101458 17.90085516836059 8 27.087761996532596 34.76438632866443 19.58941768769137 18.558433987111613 9 26.84071023684017 15.583619251837892 19.402986160259353 38.172684351062586 10-11 25.624873266766752 25.697526076357285 27.475473993658568 21.202126663217403 12-13 26.02470195448032 22.843707038893214 27.48148808181613 23.650102924810334 14-15 23.19269138816235 24.535287659843604 24.455855714635643 27.816165237358398 16-17 23.731732354270264 27.777423176554134 25.242642706534756 23.248201762640853 18-19 23.74859825763834 26.912198459438315 26.18196479994413 23.15723848297922 20-21 24.122770243837053 26.279199236564445 26.238841329770295 23.359189189828207 22-23 24.267400749589495 25.83534091336875 26.039587593018986 23.857670744022773 24-25 24.018316990135887 26.09698257048913 25.933671836354645 23.951028603020337 26-27 23.748617182454808 26.569810485571505 25.874250759765378 23.80732157220831 28-29 23.89456846233975 26.39696532801052 26.0096296533383 23.69883655631143 30-31 23.99138490616208 26.122078324094346 25.90739210626204 23.979144663481534 32-33 23.787192264419975 26.35776621197306 25.833686265959482 24.021355257647485 34-35 24.334182793004487 26.14274268427559 25.6751822361944 23.847892286525525 36-37 24.29077317761693 25.92754837588112 25.726574711941385 24.05510373456056 38-39 23.891771403695305 26.19603149448225 25.951452643806455 23.960744458015995 40-41 24.1940159056499 26.002795326865215 25.9169643172125 23.886224450272387 42-43 23.860183752635873 25.879811577536238 25.94023929594707 24.319765373880823 44-45 23.966943651604154 26.031074247674578 25.908729535264662 24.093252565456602 46-47 24.17445933796723 26.375731381237657 25.55237286113722 23.897436419657897 48-49 24.126095790114594 25.9939310085961 25.797232545739302 24.08274065555 50-51 23.90148018670909 26.15018341396565 25.917469898360366 24.030866500964894 52-53 24.062833650097286 26.025794181569665 25.78637991997217 24.12499224836088 54-55 23.938387609434663 25.894022163627927 25.851394906079094 24.316195320858313 56-57 23.713536968603602 26.22246631943434 26.054227165594902 24.009769546367153 58-59 23.9291968255579 25.950967785112116 25.848963581644192 24.270871807685797 60-61 23.871066651196305 25.93674197799256 26.14724681496634 24.044944555844797 62-63 23.81220331794677 26.010485365064532 26.145085196546493 24.032226120442207 64-65 23.823913132826046 25.83270198185563 25.976751959432498 24.366632925885824 66-67 23.634699054089715 26.04700579956799 25.952962191778035 24.365332954564263 68-69 23.608013807376906 25.85125788622736 26.289140148876694 24.251588157519034 70-71 23.5962746992842 25.809022146750543 26.13807922319617 24.456623930769084 72-73 23.67563167371916 25.968172930677618 25.82429803472821 24.531897360875014 74-75 23.67620310989082 26.019833333363646 25.836438562297026 24.46752499444851 76-77 23.753991422771392 25.918211362509297 25.963256088084712 24.3645411266346 78-79 23.682839901811985 25.810871314994188 26.20911909025143 24.2971696929424 80-81 23.76364210734219 25.998866238102003 26.015197938204704 24.222293716351107 82-83 23.64951882233738 25.981993281172787 26.04655891541374 24.321928981076084 84-85 23.64120229465425 25.781960560652788 26.164809105870145 24.412028038822818 86-87 23.653876232635795 25.68791171217704 26.305639115521302 24.352572939665865 88-89 23.63398145056333 25.760841177450555 26.246674479865735 24.35850289212038 90-91 23.641913496970673 25.852428862568566 26.167019060550523 24.33863857991024 92-93 23.543555132346874 25.53928796675306 26.259999578084546 24.657157322815515 94-95 23.516338390653427 25.57303119864815 26.371510438224288 24.539119972474136 96-97 23.629719831685872 25.413956513152225 26.434476389793637 24.521847265368265 98-99 23.500727050270854 25.505407231804284 26.23245750265688 24.761408215267984 100-101 23.316678999954352 25.394363617813692 26.53942254025474 24.749534841977212 102-103 23.256160341934372 25.101448568990026 26.637185217543596 25.005205871532006 104-105 23.03501379419462 25.432621224327978 26.559380506993485 24.972984474483923 106-107 23.32930712918187 25.15273752785973 26.469073514778025 25.048881828180374 108-109 23.251857265553014 25.32182207238703 26.309478369014155 25.1168422930458 110-111 23.20358979255036 25.37914228690153 26.580558927426722 24.836708993121384 112-113 23.222263140426733 25.42782710694655 26.685707271164944 24.664202481461782 114-115 23.060689317275415 25.64438785540745 26.366583110742848 24.92833971657428 116-117 22.93859219191388 25.40980640097275 26.759560057927125 24.892041349186243 118-119 22.950477586311532 25.553177642498152 26.424917774893014 25.071426996297298 120-121 22.87401033428547 25.618572902403425 26.596179282827947 24.91123748048316 122-123 22.79885444774577 25.694802543206034 26.68962136569546 24.816721643352732 124-125 22.717434812755506 25.804838179314043 26.51903067261906 24.95869633531139 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 303.0 1 280.0 2 219.0 3 163.5 4 142.0 5 124.5 6 101.5 7 94.5 8 92.5 9 91.0 10 89.5 11 97.0 12 117.5 13 121.5 14 119.5 15 142.5 16 181.0 17 222.0 18 283.5 19 375.0 20 478.0 21 640.5 22 885.0 23 1252.5 24 1964.0 25 3088.0 26 4686.0 27 7268.5 28 10386.0 29 13937.5 30 17920.5 31 23326.5 32 29892.0 33 34996.5 34 38670.0 35 43388.5 36 50957.0 37 57966.0 38 62902.5 39 67976.5 40 72332.5 41 77124.0 42 81909.5 43 86706.5 44 94068.0 45 100599.5 46 103858.5 47 106345.0 48 107292.5 49 107246.0 50 107289.0 51 106604.5 52 109011.0 53 114166.5 54 118710.5 55 114193.0 56 102844.5 57 94720.5 58 89712.5 59 79959.5 60 64851.0 61 53340.0 62 43409.5 63 33468.5 64 25888.0 65 19884.5 66 14668.5 67 11389.5 68 8779.5 69 6379.0 70 4759.0 71 3379.5 72 2466.5 73 1823.0 74 1431.0 75 1137.5 76 789.5 77 443.5 78 269.0 79 193.5 80 118.5 81 73.5 82 51.0 83 31.5 84 20.0 85 13.0 86 13.0 87 14.0 88 9.5 89 8.0 90 9.0 91 5.5 92 4.5 93 6.5 94 5.0 95 2.0 96 2.5 97 2.5 98 3.5 99 3.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08812081898899998 2 2.909478134180767E-4 3 3.636847667725959E-5 4 0.0023639509840218736 5 0.0012365282070268262 6 2.1821086006355755E-4 7 0.0 8 9.81948870286009E-4 9 5.818956268361534E-4 10-11 0.009328514267717086 12-13 0.001963897740572018 14-15 0.004018716672837186 16-17 0.002418503699037763 18-19 0.013710915707326864 20-21 0.017547789996777752 22-23 0.009546725127780643 24-25 0.009964962609569127 26-27 0.009655830557812422 28-29 0.007637380102224514 30-31 0.018384264960354724 32-33 0.001945713502233388 34-35 0.0015638444971221624 36-37 7.273695335451918E-4 38-39 3.455005284339661E-4 40-41 0.0016365814504766816 42-43 0.0024730564140536524 44-45 9.455803936087494E-4 46-47 0.001527476020444903 48-49 0.00901938221596038 50-51 0.004218743294562113 52-53 0.007837406723949442 54-55 0.007982880630658482 56-57 0.009964962609569127 58-59 0.008637513210849154 60-61 0.01058322671308254 62-63 0.013583626038956458 64-65 0.008764802879219562 66-67 0.013729099945665495 68-69 0.013783652660681387 70-71 0.004036900911175815 72-73 0.01347452060892468 74-75 0.013583626038956458 76-77 0.005255244879864012 78-79 0.007819222485610813 80-81 0.013801836899020016 82-83 0.017947843240227607 84-85 0.013619994515633716 86-87 0.01614760364470326 88-89 0.016002129737994218 90-91 0.01247438750030004 92-93 0.009746751749505572 94-95 0.008001064868997109 96-97 0.009128487645992157 98-99 0.006109904081779612 100-101 0.010892358764839247 102-103 0.012165255448543334 104-105 0.010510489759728023 106-107 0.0053097975948799 108-109 0.003436821046001031 110-111 0.00623719375015002 112-113 0.00896482950094449 114-115 0.013729099945665495 116-117 0.01154699134502992 118-119 0.22179315501626762 120-121 0.02640351406769046 122-123 0.026967225456187988 124-125 0.2377043635625687 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2749634.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.8641306421991 #Duplication Level Percentage of deduplicated Percentage of total 1 83.80043245838723 56.03243063770387 2 9.034356550410198 12.081487933096694 3 3.072575659055552 6.163353008253942 4 1.4220194855157913 3.8032838662112245 5 0.7530257684727866 2.517520668005339 6 0.44930964912959054 1.8025619446920949 7 0.2993111145643148 1.4009224226823398 8 0.20245687268063672 1.082968222746333 9 0.15828477203895944 0.952521630865532 >10 0.7299732744551239 8.799913861946974 >50 0.053422375483583466 2.457846915172719 >100 0.02428324706259263 2.698239170545909 >500 5.487727436606139E-4 0.20694971807700752 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 5.455271501588938E-5 0.0 24-25 0.0 0.0 0.0 2.3639509840218736E-4 0.0 26-27 0.0 0.0 0.0 4.7279019680437467E-4 0.0 28-29 0.0 0.0 0.0 0.002036634693926537 0.0 30-31 0.0 0.0 0.0 0.008492039304140114 0.0 32-33 0.0 0.0 0.0 0.02340311474181655 0.0 34-35 0.0 0.0 0.0 0.04642436047852187 0.0 36-37 0.0 0.0 0.0 0.08666607992190961 0.0 38-39 0.0 0.0 0.0 0.14860159570328269 0.0 40-41 0.0 0.0 0.0 0.2152468292143609 0.0 42-43 0.0 0.0 0.0 0.28298311702575685 0.0 44-45 0.0 0.0 0.0 0.36853995840901005 0.0 46-47 0.0 0.0 0.0 0.4741903831564492 0.0 48-49 0.0 0.0 0.0 0.5847141837786412 0.0 50-51 0.0 0.0 0.0 0.7038209448966664 0.0 52-53 0.0 0.0 0.0 0.8181816198083091 0.0 54-55 0.0 0.0 0.0 0.9510538493486769 0.0 56-57 0.0 0.0 0.0 1.1050379796001941 0.0 58-59 0.0 0.0 0.0 1.2745696336312395 0.0 60-61 0.0 0.0 0.0 1.4500839020756944 0.0 62-63 0.0 0.0 0.0 1.6333810245290827 0.0 64-65 0.0 0.0 0.0 1.82651581992367 0.0 66-67 0.0 0.0 0.0 2.0487635808984033 0.0 68-69 0.0 0.0 0.0 2.2958328272053663 0.0 70-71 0.0 0.0 0.0 2.5547763811474544 0.0 72-73 0.0 0.0 0.0 2.820502655989852 0.0 74-75 0.0 0.0 0.0 3.0958302086750455 0.0 76-77 0.0 0.0 0.0 3.4021982562042803 0.0 78-79 0.0 0.0 0.0 3.7448074907424043 0.0 80-81 0.0 0.0 0.0 4.104328066935453 0.0 82-83 0.0 0.0 0.0 4.472140655810919 0.0 84-85 0.0 0.0 0.0 4.855919005947701 0.0 86-87 0.0 0.0 0.0 5.261009283417357 0.0 88-89 0.0 0.0 0.0 5.701195140880568 0.0 90-91 0.0 0.0 0.0 6.167875433603163 0.0 92-93 0.0 0.0 0.0 6.653921940156399 0.0 94-95 0.0 0.0 0.0 7.155515970489163 0.0 96-97 0.0 1.8184238338629795E-5 0.0 7.673857684331805 0.0 98-99 0.0 3.636847667725959E-5 0.0 8.221548758852997 0.0 100-101 0.0 3.636847667725959E-5 0.0 8.816537037292964 0.0 102-103 0.0 3.636847667725959E-5 0.0 9.427163760704152 0.0 104-105 0.0 3.636847667725959E-5 0.0 10.045518785409257 0.0 106-107 0.0 3.636847667725959E-5 0.0 10.676311829137987 0.0 108-109 0.0 3.636847667725959E-5 0.0 11.341800399616822 0.0 110-111 0.0 3.636847667725959E-5 0.0 12.042257260420842 0.0 112-113 0.0 3.636847667725959E-5 0.0 12.754606613098325 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3165 0.0 44.202118 1 ATCAACG 3310 0.0 40.07324 3 GTACATG 9715 0.0 39.524452 1 GGTATCA 2285 0.0 38.29835 1 CAACGCA 3450 0.0 38.277454 5 TCAACGC 3550 0.0 37.030304 4 TACATGG 10265 0.0 36.853245 2 AACGCAG 3575 0.0 36.437252 6 ACATGGG 10050 0.0 36.339577 3 TATCAAC 3910 0.0 34.53239 2 CATGGGG 7005 0.0 31.418608 4 ACGCAGA 4565 0.0 28.274605 7 CGCAGAG 4740 0.0 27.356195 8 CATGGGA 3925 0.0 26.066465 4 ATGGGGA 3840 0.0 23.236418 5 AGTACAT 6755 0.0 22.365875 2 CGTATAG 160 2.6031205E-4 22.32442 1 TAAGGTG 1155 0.0 21.630993 5 GAGTACA 7440 0.0 21.36423 1 TATTCCG 405 2.0008883E-11 20.562796 5 >>END_MODULE