FastQCFastQC Report
Thu 26 May 2016
SRR1364459_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364459_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2749634
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33000.054.89481
GTATCAA47450.048.8522871
ATCAACG60450.038.3282743
CAACGCA62200.037.0213175
TCAACGC62700.036.7628674
TATCAAC64750.035.692232
GTACATG93100.035.139391
AACGCAG65900.035.031116
TACATGG98000.033.4286162
ACATGGG98800.032.1318863
CATGGGG70550.029.7174534
ACGCAGA78050.029.5016087
CGCAGAG80050.028.614318
ATGGGGA38500.024.7280885
GCAGAGT96100.023.2781439
AGTACAT69550.022.6950152
GAGTACA78400.021.9661181
CAGAGTA90100.021.9228610-11
AGAGTAC88400.021.7050810-11
GAGTACT46500.021.55911312-13
Produced by FastQC (version 0.11.5)