##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364459_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2749634 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.944532617795677 33.0 31.0 34.0 30.0 34.0 2 31.882581099884565 33.0 31.0 34.0 30.0 34.0 3 32.10460701315157 33.0 31.0 34.0 30.0 34.0 4 35.78865841781124 37.0 35.0 37.0 35.0 37.0 5 35.72256853093903 37.0 35.0 37.0 33.0 37.0 6 35.78143527465837 37.0 35.0 37.0 33.0 37.0 7 35.68813122037333 37.0 35.0 37.0 33.0 37.0 8 35.700148456121795 37.0 35.0 37.0 33.0 37.0 9 37.347661907002895 39.0 37.0 39.0 34.0 39.0 10-11 37.29581046059221 39.0 37.0 39.0 33.5 39.0 12-13 37.26808567976684 39.0 37.0 39.0 33.0 39.0 14-15 38.589400989368045 40.0 38.0 41.0 34.0 41.0 16-17 38.60490268886696 40.0 38.0 41.0 34.0 41.0 18-19 38.637473205524806 40.0 38.0 41.0 34.0 41.0 20-21 38.63210958258445 40.0 38.0 41.0 34.0 41.0 22-23 38.60330738563751 40.0 38.0 41.0 34.0 41.0 24-25 38.556684089591556 40.0 38.0 41.0 34.0 41.0 26-27 38.449610384509356 40.0 38.0 41.0 34.0 41.0 28-29 38.37923374529119 40.0 38.0 41.0 34.0 41.0 30-31 38.28601570245349 40.0 38.0 41.0 33.0 41.0 32-33 38.18945739687537 40.0 38.0 41.0 33.0 41.0 34-35 38.103515231481715 40.0 37.5 41.0 33.0 41.0 36-37 38.01712646119447 40.0 37.0 41.0 33.0 41.0 38-39 37.930817883398305 40.0 37.0 41.0 33.0 41.0 40-41 37.82884994875681 40.0 37.0 41.0 33.0 41.0 42-43 37.71074313890503 40.0 37.0 41.0 32.5 41.0 44-45 37.571210204703604 40.0 37.0 41.0 32.0 41.0 46-47 37.41453335243891 40.0 36.5 41.0 31.5 41.0 48-49 37.23098456012691 40.0 36.0 41.0 31.0 41.0 50-51 37.013393964433085 39.5 36.0 41.0 31.0 41.0 52-53 36.87841690930502 39.0 35.5 41.0 31.0 41.0 54-55 36.7143456183623 39.0 35.0 41.0 31.0 41.0 56-57 36.4866740446183 39.0 35.0 41.0 30.0 41.0 58-59 36.25426474941756 38.5 35.0 40.0 30.0 41.0 60-61 35.99075858823393 38.0 35.0 40.0 29.5 41.0 62-63 36.01544969257726 38.0 35.0 40.0 30.0 41.0 64-65 35.78097739553701 37.0 35.0 40.0 30.0 41.0 66-67 35.4872793979126 37.0 34.5 39.5 30.0 41.0 68-69 35.15479605649334 36.0 34.0 39.0 29.0 41.0 70-71 34.819803290183344 36.0 34.0 39.0 29.0 41.0 72-73 34.47472645450267 35.0 34.0 38.5 29.0 40.0 74-75 34.14307249619404 35.0 34.0 37.0 29.0 39.5 76-77 33.83515115102592 35.0 34.0 37.0 29.0 39.0 78-79 33.5301116439497 35.0 33.5 36.0 29.0 39.0 80-81 33.26878031767137 35.0 33.0 36.0 29.0 37.5 82-83 33.02778133380661 35.0 33.0 35.5 28.5 37.0 84-85 32.82732283642113 35.0 33.0 35.0 28.0 37.0 86-87 32.664500802652285 35.0 33.0 35.0 27.5 36.0 88-89 32.50773011971775 35.0 33.0 35.0 27.0 36.0 90-91 32.40676395476635 35.0 33.0 35.0 27.0 36.0 92-93 32.29947222066646 35.0 33.0 35.0 27.0 35.5 94-95 32.199361805971265 35.0 33.0 35.0 27.0 35.0 96-97 32.15215497771703 35.0 33.0 35.0 27.0 35.0 98-99 32.08685956021783 35.0 33.0 35.0 27.0 35.0 100-101 32.091935508507675 35.0 33.0 35.0 27.0 35.0 102-103 30.952153450241013 33.5 30.5 34.5 24.5 35.0 104-105 31.942520713665893 35.0 32.5 35.0 26.0 35.0 106-107 32.141620121077935 35.0 33.0 35.0 27.0 35.0 108-109 32.150191807346 35.0 33.0 35.0 27.0 35.0 110-111 32.09649647916777 35.0 33.0 35.0 27.0 35.0 112-113 32.04504199468002 35.0 33.0 35.0 27.0 35.0 114-115 31.942792386186667 35.0 33.0 35.0 27.0 35.0 116-117 31.866738991443953 35.0 33.0 35.0 27.0 35.0 118-119 31.768810139822243 35.0 33.0 35.0 25.0 35.0 120-121 31.652112608441705 35.0 33.0 35.0 25.0 35.0 122-123 31.470738832877394 35.0 33.0 35.0 25.0 35.0 124-125 30.434676942458523 34.5 31.0 35.0 21.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27652548928233855 1101 2 0.46857262713496795 1101 3 0.35328834161197165 1101 4 0.23489388791852406 1101 5 0.2020208394635432 1101 6 0.18735546012257487 1101 7 0.2633834072042589 1101 8 0.2746028599830481 1101 9 0.40038100058844606 1101 10-11 0.39713613496306266 1101 12-13 0.3622920570793511 1101 14-15 0.3727094159937465 1101 16-17 0.3142715709318722 1101 18-19 0.2559478251774152 1101 20-21 0.24338205088533016 1101 22-23 0.3590447049469674 1101 24-25 0.28391699519112024 1101 26-27 0.2813764741108713 1101 28-29 0.28467481548661056 1101 30-31 0.24473253736038458 1101 32-33 0.278111299874638 1101 34-35 0.28646983306397544 1101 36-37 0.23242493084262605 1101 38-39 0.31726189480951916 1101 40-41 0.24753815374533872 1101 42-43 0.2134952578578222 1101 44-45 0.2190645621303986 1101 46-47 0.3062012005053134 1101 48-49 0.16168520338580805 1101 50-51 0.24288694192639326 1101 52-53 0.21594809941950643 1101 54-55 0.10653699555026463 1101 56-57 0.21663541568090494 1101 58-59 0.2078344657407527 1101 60-61 0.06359003466278779 1101 62-63 0.3425470961738242 1101 64-65 0.4292042182565581 1101 66-67 0.3630261395931669 1101 68-69 0.42049667037713334 1101 70-71 0.3651980762294116 1101 72-73 0.35503347822150033 1101 74-75 0.359002329083836 1101 76-77 0.36152413845115206 1101 78-79 0.33473424049234524 1101 80-81 0.15010880467102083 1101 82-83 0.2749262255735445 1101 84-85 0.19210879289178706 1101 86-87 0.3061490488343992 1101 88-89 0.3382301249471027 1101 90-91 0.3531915436605857 1101 92-93 0.33890122977788906 1101 94-95 0.41962298003366527 1101 96-97 0.24795270836825978 1101 98-99 0.2599531110921589 1101 100-101 0.2568455729135479 1101 102-103 0.43575338550815346 1101 104-105 0.4105649428348599 1101 106-107 0.35199927532084274 1101 108-109 0.31342520524427897 1101 110-111 0.35752678096758217 1101 112-113 0.40795783354396065 1101 114-115 0.34921095525010415 1101 116-117 0.35941228174469586 1101 118-119 0.323019890637088 1101 120-121 0.28691653237557446 1101 122-123 0.39955695862738594 1101 124-125 0.21139790602919817 1102 1 0.3838976420621911 1102 2 0.5467835484353571 1102 3 0.331333148892206 1102 4 0.26015743226277976 1102 5 0.16468027359713489 1102 6 0.2452978748772665 1102 7 0.3031090541278587 1102 8 0.26977313011362725 1102 9 0.34727928091181326 1102 10-11 0.37162640686704407 1102 12-13 0.3699522629265459 1102 14-15 0.4271130911997645 1102 16-17 0.4293143613249697 1102 18-19 0.369463013828792 1102 20-21 0.39305780077520325 1102 22-23 0.3419499446808061 1102 24-25 0.36713466039117293 1102 26-27 0.3389905549561121 1102 28-29 0.41477126664721453 1102 30-31 0.42091247086261063 1102 32-33 0.37765233773103546 1102 34-35 0.4002573145179511 1102 36-37 0.35737502432547075 1102 38-39 0.2679216269596978 1102 40-41 0.32949058204710013 1102 42-43 0.4079506082395028 1102 44-45 0.3097698052308502 1102 46-47 0.19196447148988227 1102 48-49 0.2508861976753636 1102 50-51 0.363083498922002 1102 52-53 0.3124654365405206 1102 54-55 0.22564355153589588 1102 56-57 0.22866110817236063 1102 58-59 0.08843116750082913 1102 60-61 0.1939724110475467 1102 62-63 0.30397528555589304 1102 64-65 0.42395357255394117 1102 66-67 0.34586425446872937 1102 68-69 0.2540742315060953 1102 70-71 0.22364677541189337 1102 72-73 0.28609091433997236 1102 74-75 0.32127972942134875 1102 76-77 0.2773679699345024 1102 78-79 0.22681258258125325 1102 80-81 0.1349124776484274 1102 82-83 0.18134255593430026 1102 84-85 0.12637387852947057 1102 86-87 0.23173348260050375 1102 88-89 0.24821989826008917 1102 90-91 0.2859520841303862 1102 92-93 0.23123695791279175 1102 94-95 0.22430034092013784 1102 96-97 0.22073584546584613 1102 98-99 0.18283657688521515 1102 100-101 0.07330579137369142 1102 102-103 0.22589304314707448 1102 104-105 0.14384264403398817 1102 106-107 0.08745576347627804 1102 108-109 0.06451274649123917 1102 110-111 0.12043268335565216 1102 112-113 0.11637453209863935 1102 114-115 0.16625890889323713 1102 116-117 0.1273264098173783 1102 118-119 0.07692131187037887 1102 120-121 0.09315077507440961 1102 122-123 -0.0632437785191371 1102 124-125 -0.16321022112265027 1103 1 0.26175052505184127 1103 2 0.37659147694147777 1103 3 0.23320892851985775 1103 4 0.16639385430960374 1103 5 0.08672565706309854 1103 6 0.18261035982393992 1103 7 0.2491070554052186 1103 8 0.2598463806621396 1103 9 0.28420406399408904 1103 10-11 0.2817702537850195 1103 12-13 0.23540575607781022 1103 14-15 0.3554766789787962 1103 16-17 0.40373673287888323 1103 18-19 0.23583588344569506 1103 20-21 0.2815260219240443 1103 22-23 0.18653128538273478 1103 24-25 0.29242353452796976 1103 26-27 0.2945198449901767 1103 28-29 0.2804571352707441 1103 30-31 0.30505515904869895 1103 32-33 0.23135264077477302 1103 34-35 0.25533560381268217 1103 36-37 0.2039776152356012 1103 38-39 0.1996639007823049 1103 40-41 0.202521157231061 1103 42-43 0.19242342117800604 1103 44-45 0.21814523795789853 1103 46-47 0.1973903803514787 1103 48-49 0.28707110588150897 1103 50-51 0.23802649099062734 1103 52-53 0.212625016808758 1103 54-55 0.2085788578689005 1103 56-57 0.06425134247106001 1103 58-59 -0.10136165674937558 1103 60-61 0.0133758977880305 1103 62-63 0.1647505934226885 1103 64-65 0.34939274032801393 1103 66-67 0.20306169303616883 1103 68-69 0.1684437651255024 1103 70-71 0.22441220350161473 1103 72-73 0.2883412447642897 1103 74-75 0.3094919391242641 1103 76-77 0.2625622941853152 1103 78-79 0.22346756806414447 1103 80-81 0.3029860888777023 1103 82-83 0.2685148427102533 1103 84-85 0.3072528551547222 1103 86-87 0.2632949067742629 1103 88-89 0.27638669933326554 1103 90-91 0.18572294400051703 1103 92-93 0.05453262147552351 1103 94-95 0.235905184587196 1103 96-97 0.14380404648365896 1103 98-99 0.1562285218412498 1103 100-101 0.2001745610928083 1103 102-103 0.20907774045682714 1103 104-105 0.20654651554057324 1103 106-107 0.20365967632334758 1103 108-109 0.1457187023971187 1103 110-111 0.12749612482531347 1103 112-113 0.13224544433338536 1103 114-115 0.12711728717185977 1103 116-117 0.1420695955272322 1103 118-119 0.0891456936503694 1103 120-121 0.11827210041444758 1103 122-123 0.14332010211829527 1103 124-125 -0.03789426597168344 1104 1 0.2791259329713718 1104 2 0.3801900806049261 1104 3 0.2618473127656884 1104 4 0.16730004249139796 1104 5 0.15913358714777104 1104 6 0.21301339546053555 1104 7 0.24632206285711788 1104 8 0.20033697462111633 1104 9 0.1905597522362683 1104 10-11 0.2305112584484661 1104 12-13 0.27493657386338555 1104 14-15 0.2613376456967842 1104 16-17 0.3239382624232263 1104 18-19 0.3496537874633887 1104 20-21 0.29749330862448176 1104 22-23 0.2986058207678184 1104 24-25 0.29435528195381266 1104 26-27 0.22559363928968423 1104 28-29 0.2641413214192525 1104 30-31 0.19578988910945583 1104 32-33 0.19442989894577067 1104 34-35 0.22715377283882532 1104 36-37 0.17759016115768844 1104 38-39 0.03138538276583347 1104 40-41 0.04051003039403156 1104 42-43 0.026426760753494705 1104 44-45 0.11724382813639522 1104 46-47 0.17931516251101698 1104 48-49 0.11533273254088527 1104 50-51 0.1913658905940494 1104 52-53 0.08575559327249493 1104 54-55 0.07306754986458941 1104 56-57 0.16125572097348595 1104 58-59 0.005583470085305464 1104 60-61 -0.006845947116360662 1104 62-63 0.26242449935407564 1104 64-65 0.34363669233100325 1104 66-67 0.2878367817729597 1104 68-69 0.3504699188796536 1104 70-71 0.314819337388343 1104 72-73 0.2549206013097063 1104 74-75 0.19568992388744988 1104 76-77 0.23278938649151115 1104 78-79 0.22453613255090232 1104 80-81 0.24242377261005288 1104 82-83 0.26093026573292377 1104 84-85 0.20311247924141185 1104 86-87 0.20714295843448838 1104 88-89 0.29548152545371664 1104 90-91 0.16415908341372187 1104 92-93 0.1952803674379595 1104 94-95 0.2345574231463985 1104 96-97 0.25413535981159185 1104 98-99 0.24912137048273308 1104 100-101 0.18053353614332224 1104 102-103 0.19071379825681234 1104 104-105 0.2095226425007226 1104 106-107 0.1810800184187613 1104 108-109 0.10099025983769394 1104 110-111 0.1299006367700386 1104 112-113 0.0022619663202902984 1104 114-115 0.014571628913156331 1104 116-117 -0.05530825727112898 1104 118-119 -0.1951557804298112 1104 120-121 -0.004098863336853498 1104 122-123 -0.08477199535321489 1104 124-125 -0.19316083984630694 1105 1 0.19429835114891958 1105 2 0.3679104188818556 1105 3 0.1622919458456451 1105 4 0.1352558662249379 1105 5 0.09541323514325484 1105 6 0.0818606738910006 1105 7 0.12858383539992957 1105 8 0.1045793101422916 1105 9 0.10844567499801627 1105 10-11 0.21612345610474648 1105 12-13 0.19417477620022083 1105 14-15 0.21477632398696045 1105 16-17 0.22504902090544476 1105 18-19 0.17842273652285456 1105 20-21 0.24156755212629122 1105 22-23 0.26593523907661165 1105 24-25 0.27141381620450034 1105 26-27 0.22691919508385183 1105 28-29 0.24950808224551935 1105 30-31 0.22698984707154324 1105 32-33 0.21024747982923486 1105 34-35 0.19929364049478693 1105 36-37 0.18841537407168119 1105 38-39 0.16792516848386896 1105 40-41 0.13910775765742756 1105 42-43 0.12376824527133579 1105 44-45 0.26201068452691345 1105 46-47 0.1265694515512692 1105 48-49 0.026562520993749672 1105 50-51 0.08690429066997041 1105 52-53 0.09788793663696538 1105 54-55 0.05768584791305642 1105 56-57 -0.13525444579359913 1105 58-59 -0.021564318780619374 1105 60-61 0.014184205691343266 1105 62-63 0.1708137468073403 1105 64-65 0.15733852053580222 1105 66-67 0.131329183864473 1105 68-69 0.25693668566391636 1105 70-71 0.2706068109789115 1105 72-73 0.21831450685863985 1105 74-75 0.22891298458626608 1105 76-77 0.2216996081464231 1105 78-79 0.10916872641565334 1105 80-81 0.11242920344493967 1105 82-83 0.10621389253369529 1105 84-85 0.12113598727945174 1105 86-87 0.1200010971844776 1105 88-89 0.17610805577696453 1105 90-91 0.025406146681177688 1105 92-93 0.039775952134874615 1105 94-95 6.621158929647208E-4 1105 96-97 0.08926538450830179 1105 98-99 0.11901003246016018 1105 100-101 0.12733990648506932 1105 102-103 0.05011489525958268 1105 104-105 0.052547181514096764 1105 106-107 0.009126131399817439 1105 108-109 0.055568299494773044 1105 110-111 0.15439624509640737 1105 112-113 0.07327616643730295 1105 114-115 0.041272455510643624 1105 116-117 0.026483981253978328 1105 118-119 -0.08478068600344102 1105 120-121 -0.1468384255765649 1105 122-123 -0.18061241916200288 1105 124-125 -0.2540705533555574 1106 1 0.21835932931144342 1106 2 0.4175947178926265 1106 3 0.22625731153594586 1106 4 0.1809722959742004 1106 5 0.07560052841530762 1106 6 0.10223385540326291 1106 7 0.13832496844332098 1106 8 0.15657394729282004 1106 9 0.2677515145641465 1106 10-11 0.26607382134987034 1106 12-13 0.24849205513139339 1106 14-15 0.26414888498926814 1106 16-17 0.20726122432660077 1106 18-19 0.21883176557169293 1106 20-21 0.23448741098814452 1106 22-23 0.21248465277791695 1106 24-25 0.23720395464957278 1106 26-27 0.1898438485207663 1106 28-29 0.13179063612763997 1106 30-31 0.2250076151767999 1106 32-33 0.21212504838591428 1106 34-35 0.15695178635917273 1106 36-37 0.18942493174864694 1106 38-39 0.2089079097163875 1106 40-41 0.1655315841413767 1106 42-43 0.20223345175581642 1106 44-45 0.08090386437159225 1106 46-47 0.13718916891576782 1106 48-49 0.09267528397240454 1106 50-51 0.09677562809167028 1106 52-53 -0.015725091776261024 1106 54-55 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46-47 -0.6851584580842882 2216 48-49 -0.6997869343715948 2216 50-51 -0.6006196957676835 2216 52-53 -0.46982826065352157 2216 54-55 -0.5459162027319096 2216 56-57 -0.5969996264294934 2216 58-59 -0.5479276552246546 2216 60-61 -0.5945447379595876 2216 62-63 -0.7109002047174471 2216 64-65 -0.8014910724139384 2216 66-67 -0.7579315137614984 2216 68-69 -0.7498199969837671 2216 70-71 -0.6814487543516066 2216 72-73 -0.6736953177413554 2216 74-75 -0.6546745299522669 2216 76-77 -0.7363149241849385 2216 78-79 -0.8097657740234041 2216 80-81 -0.7450144140468069 2216 82-83 -0.7693118322782269 2216 84-85 -0.8044058760081967 2216 86-87 -0.7047801788983428 2216 88-89 -0.5864083435110103 2216 90-91 -0.546377701507879 2216 92-93 -0.5795575795773189 2216 94-95 -0.6180216935913023 2216 96-97 -0.5969188304146655 2216 98-99 -0.5716676269053167 2216 100-101 -0.5907369922421779 2216 102-103 -0.5544804610936254 2216 104-105 -0.5836242874055273 2216 106-107 -0.7297836340895323 2216 108-109 -0.6448755991385546 2216 110-111 -0.6937062634813174 2216 112-113 -0.6257674828467614 2216 114-115 -0.7316003457017501 2216 116-117 -0.680258259154165 2216 118-119 -0.7227929155347574 2216 120-121 -0.7261596586836454 2216 122-123 -0.670809551395422 2216 124-125 -0.5613476667727504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 113.0 3 18.0 4 42.0 5 137.0 6 361.0 7 907.0 8 1385.0 9 2422.0 10 3656.0 11 4869.0 12 6071.0 13 7631.0 14 9902.0 15 8090.0 16 5658.0 17 5647.0 18 5833.0 19 6128.0 20 6262.0 21 6803.0 22 7422.0 23 9024.0 24 12113.0 25 16658.0 26 22826.0 27 30492.0 28 40071.0 29 50947.0 30 63909.0 31 79897.0 32 99875.0 33 128508.0 34 172854.0 35 255033.0 36 486561.0 37 872251.0 38 318677.0 39 581.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.758001912338074 18.605928830170114 12.83100258034658 25.805066677145234 2 16.13242877868756 20.040931182585297 37.840612796613776 25.986027242113362 3 18.975265955802474 23.737030438123792 29.27497744226365 28.012726163810083 4 13.442264827791034 16.083234212054993 35.70127637147695 34.773224588677024 5 13.891190349239233 37.648456316181125 34.35966745494209 14.100685879637542 6 34.185058811463634 35.554222852932426 16.57704261730834 13.683675718295598 7 29.649151009891973 31.689156800031427 20.568488402627093 18.093203787449507 8 27.16863408002665 34.7925214774039 19.33573704718519 18.703107395384258 9 26.8274250318406 15.108010738883792 19.09286835993445 38.971695869341154 10-11 25.65079570590122 25.579986281810598 27.46911043433417 21.300107577954012 12-13 26.175847403690817 22.635157988299532 27.66620939368658 23.52278521432307 14-15 23.168228935196467 24.357078069299405 24.80641059864695 27.66828239685718 16-17 23.4642865195877 27.7009594731517 25.56520249604129 23.269551511219312 18-19 23.475323243255826 26.498727829760494 26.95342504015083 23.072523886832858 20-21 23.91932889977357 25.838711624892625 27.02092351200196 23.221035963331847 22-23 23.958134064388208 25.502212294436276 26.827225005218875 23.71242863595664 24-25 23.7976581610498 25.7866865190058 26.762743696070096 23.652911623874303 26-27 23.501535210742567 26.225290926299884 26.779421538774866 23.493752324182683 28-29 23.589968795688133 25.986609058706296 26.917937751398373 23.505484394207198 30-31 23.66221847220568 25.7503816439923 26.817439152900356 23.769960730901662 32-33 23.612015271850726 25.913903450422854 26.742413717607505 23.73166756011891 34-35 24.09371574544103 25.706512212170786 26.572718405431413 23.627053636956774 36-37 24.06623936131136 25.55234623953588 26.60354068941539 23.777873709737367 38-39 23.730053478036258 25.85746427660268 26.710850737016408 23.701631508344658 40-41 23.967304483813628 25.704931149389232 26.63879001131978 23.688974355477363 42-43 23.749857480124955 25.568747496241752 26.569632528754468 24.111762494878832 44-45 23.805240988437006 25.695474379499235 26.56077136084293 23.938513271220824 46-47 24.09940741204102 26.067869396436034 26.038247272182407 23.794475919340538 48-49 24.101062177729837 25.804943494297788 26.185121365243518 23.908872962728857 50-51 23.932403367139045 25.94188899322601 26.24609311639294 23.879614523242 52-53 24.00517668897024 25.79477850506649 26.176229272695927 24.02381553326734 54-55 23.892743543322492 25.67468979507818 26.177865854146404 24.25470080745292 56-57 23.678055617786285 26.065963928158904 26.335855375134926 23.92012507891988 58-59 23.80167339121757 25.73355430884055 26.263262712819852 24.20150958712203 60-61 23.795203234188243 25.76767328326951 26.513302639434087 23.923820843108157 62-63 23.67297165682107 25.89931485102856 26.48314076740031 23.944572724750067 64-65 23.659530686629566 25.692692191033423 26.360035553822797 24.28774156851421 66-67 23.498117748805022 25.94982675869834 26.31336618377798 24.238689308718655 68-69 23.470305502477785 25.7383891819784 26.612214571102914 24.179090744440895 70-71 23.475869879409405 25.68520028483791 26.416788561677663 24.42214127407502 72-73 23.620816443206625 25.793869293149562 26.00473372092431 24.580580542719506 74-75 23.63096324819958 25.907811730579418 25.99638715552688 24.464837865694125 76-77 23.69148039339054 25.801852173780222 26.118439763255765 24.388227669573478 78-79 23.684788593681922 25.692164848121607 26.23900126344088 24.384045294755595 80-81 23.723393731674832 25.909011890309763 26.080471073604706 24.287123304410695 82-83 23.67197301022118 25.86319231272095 26.03006909829983 24.434765578758043 84-85 23.692753290074243 25.680781514921623 26.098818970088384 24.527646224915753 86-87 23.714628928795616 25.573785456537124 26.22901811659297 24.48256749807429 88-89 23.558735453518544 25.659760535402164 26.1952318017598 24.586272209319496 90-91 23.57747172436718 25.773422523547723 26.08622836026242 24.56287739182268 92-93 23.49407865570228 25.417010804003016 26.20406243896904 24.88484810132567 94-95 23.44567412750359 25.4782971274805 26.214724956080364 24.861303788935544 96-97 23.56252726576086 25.328521163322158 26.261053373516784 24.8478981974002 98-99 23.35425922524492 25.422248975680017 26.109548135310263 25.113943663764804 100-101 23.26589747814855 25.241194399248347 26.3338305813728 25.159077541230303 102-103 23.16727568796553 25.00295978349843 26.38193700962389 25.447827518912153 104-105 23.1039614070188 25.22871756860436 26.249042785901732 25.418278238475107 106-107 23.31446242461927 25.118234921080635 26.116418636278738 25.45088401802136 108-109 23.22385613456113 25.20354996892804 25.971755708392447 25.60083818811838 110-111 23.150079179702647 25.262162286020217 26.164054379034003 25.423704155243136 112-113 23.174735088679597 25.28029590780886 26.28393110759937 25.261037895912175 114-115 23.031397979298355 25.551310401012532 25.894967885537216 25.522323734151897 116-117 22.93965305927989 25.28972946944939 26.260713244017204 25.509904227253514 118-119 22.899052352552562 25.404963670150387 25.919802472979235 25.77618150431781 120-121 22.814406112543733 25.490880663887015 26.068323147397038 25.626390076172218 122-123 22.689143477696362 25.551903736481307 26.09379699514733 25.665155790674998 124-125 22.651858025790638 25.617594140961735 25.945493350427412 25.785054482820218 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 16.0 1 21.5 2 68.5 3 103.5 4 70.0 5 50.5 6 114.0 7 331.5 8 591.0 9 676.0 10 526.0 11 374.0 12 374.5 13 424.0 14 469.5 15 523.5 16 617.5 17 723.0 18 836.5 19 973.0 20 1099.5 21 1252.0 22 1487.5 23 1958.0 24 2763.5 25 3793.5 26 5396.5 27 8075.0 28 11400.5 29 15048.0 30 18963.5 31 24364.0 32 30572.0 33 35510.5 34 38752.0 35 43112.5 36 50606.5 37 56896.0 38 61537.5 39 67015.5 40 71424.5 41 76471.5 42 81659.5 43 86082.0 44 93157.5 45 99615.0 46 102618.0 47 105309.5 48 106344.5 49 106212.0 50 106543.0 51 105680.5 52 108334.0 53 114152.5 54 119431.5 55 114606.5 56 102892.0 57 95469.5 58 90449.5 59 80519.5 60 65478.0 61 53762.5 62 43260.5 63 32816.0 64 25263.0 65 19230.0 66 13982.5 67 11045.5 68 8490.5 69 6067.0 70 4478.0 71 3157.5 72 2330.0 73 1724.0 74 1390.5 75 1112.5 76 712.5 77 375.0 78 238.5 79 148.0 80 67.5 81 27.5 82 12.5 83 5.0 84 2.5 85 1.5 86 1.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.042405643805684685 2 0.005127955211493603 3 8.364749635769706E-4 4 0.0784831726695262 5 0.00625537798848865 6 0.0 7 0.004800638921398266 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 7.273695335451918E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.182374817884853E-4 28-29 5.091586734816343E-4 30-31 6.000798651747833E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.8184238338629795E-5 40-41 7.819222485610812E-4 42-43 6.364483418520428E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.636847667725959E-4 58-59 2.909478134180767E-4 60-61 3.8186900511122575E-4 62-63 4.727901968043747E-4 64-65 0.0 66-67 3.636847667725959E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.8184238338629795E-5 84-85 0.0 86-87 0.0 88-89 0.0 90-91 1.8184238338629795E-4 92-93 1.8184238338629795E-4 94-95 3.455005284339661E-4 96-97 0.002818556942487618 98-99 0.009055750692637638 100-101 0.018293343768661575 102-103 0.010092252277939537 104-105 0.004309664486255261 106-107 0.012383466308606891 108-109 0.01347452060892468 110-111 0.006982747522033842 112-113 0.004691533491366488 114-115 0.003418636807662402 116-117 0.0 118-119 0.0012910809220427154 120-121 3.455005284339661E-4 122-123 5.455271501588939E-4 124-125 0.03358628821144923 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2749634.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.45660852724997 #Duplication Level Percentage of deduplicated Percentage of total 1 79.37267566462738 47.98602780416758 2 11.519449519674684 13.9285370012078 3 3.867852618875678 7.015117548613962 4 1.8032193084415922 4.360660952769269 5 0.956201570587827 2.8904352013084917 6 0.5828415479710543 2.1141973979461444 7 0.37773579228948756 1.598563744482331 8 0.2619801140252904 1.2670743356441032 9 0.18370221231226444 0.9995411461817097 >10 0.9669109583426408 10.516888182448398 >50 0.07137451974638195 2.9763123667457294 >100 0.03480747520678473 3.627299541888566 >500 9.365234242270885E-4 0.4035993191001813 >1k 3.1217447474236285E-4 0.31574545749572286 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 5.455271501588938E-5 0.0 24-25 0.0 0.0 0.0 2.3639509840218736E-4 0.0 26-27 0.0 0.0 0.0 4.7279019680437467E-4 0.0 28-29 0.0 0.0 0.0 0.002036634693926537 0.0 30-31 0.0 0.0 0.0 0.008728434402542302 0.0 32-33 0.0 0.0 0.0 0.024294142420409406 0.0 34-35 0.0 0.0 0.0 0.0479700207373054 0.0 36-37 0.0 0.0 0.0 0.08926642600433367 0.0 38-39 0.0 0.0 0.0 0.15254757542276537 0.0 40-41 0.0 0.0 0.0 0.2205566268092408 0.0 42-43 0.0 0.0 0.0 0.2890384683925206 0.0 44-45 0.0 0.0 0.0 0.3758500222211393 0.0 46-47 0.0 0.0 0.0 0.48271879093726655 0.0 48-49 0.0 0.0 0.0 0.5943700143364534 0.0 50-51 0.0 0.0 0.0 0.7143132504180556 0.0 52-53 0.0 0.0 0.0 0.829728611153339 0.0 54-55 0.0 0.0 0.0 0.9635100526106384 0.0 56-57 0.0 0.0 0.0 1.1185670529241345 0.0 58-59 0.0 0.0 0.0 1.2887715237737094 0.0 60-61 0.0 0.0 0.0 1.4652495568501118 0.0 62-63 0.0 0.0 0.0 1.6496013651271406 0.0 64-65 0.0 0.0 0.0 1.8436271882003203 0.0 66-67 0.0 0.0 0.0 2.0674024251954988 0.0 68-69 0.0 0.0 0.0 2.3158536736161976 0.0 70-71 0.0 0.0 0.0 2.5761428611953443 0.0 72-73 0.0 0.0 0.0 2.8436330071565887 0.0 74-75 0.0 0.0 0.0 3.120706246722291 0.0 76-77 0.0 0.0 0.0 3.4300383251007225 0.0 78-79 0.0 0.0 0.0 3.7768117502183927 0.0 80-81 0.0 0.0 0.0 4.1409693071877935 0.0 82-83 0.0 0.0 0.0 4.514909984383376 0.0 84-85 0.0 0.0 0.0 4.904016316353377 0.0 86-87 0.0 0.0 0.0 5.312816178444113 0.0 88-89 0.0 0.0 0.0 5.759111939989104 0.0 90-91 0.0 0.0 0.0 6.232265821560251 0.0 92-93 0.0 0.0 0.0 6.72584060278568 0.0 94-95 0.0 0.0 0.0 7.232708062236647 0.0 96-97 0.0 0.0 0.0 7.755941336192381 0.0 98-99 0.0 0.0 0.0 8.311197053862442 0.0 100-101 0.0 0.0 0.0 8.913259001016137 0.0 102-103 0.0 0.0 0.0 9.52950465407396 0.0 104-105 0.0 0.0 0.0 10.153751372000784 0.0 106-107 0.0 0.0 0.0 10.790090608422794 0.0 108-109 0.0 0.0 0.0 11.46245282099363 0.0 110-111 0.0 0.0 0.0 12.17167448467687 0.0 112-113 0.0 0.0 0.0 12.89122479573645 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3300 0.0 54.8948 1 GTATCAA 4745 0.0 48.852287 1 ATCAACG 6045 0.0 38.328274 3 CAACGCA 6220 0.0 37.021317 5 TCAACGC 6270 0.0 36.762867 4 TATCAAC 6475 0.0 35.69223 2 GTACATG 9310 0.0 35.13939 1 AACGCAG 6590 0.0 35.03111 6 TACATGG 9800 0.0 33.428616 2 ACATGGG 9880 0.0 32.131886 3 CATGGGG 7055 0.0 29.717453 4 ACGCAGA 7805 0.0 29.501608 7 CGCAGAG 8005 0.0 28.61431 8 ATGGGGA 3850 0.0 24.728088 5 GCAGAGT 9610 0.0 23.278143 9 AGTACAT 6955 0.0 22.695015 2 GAGTACA 7840 0.0 21.966118 1 CAGAGTA 9010 0.0 21.92286 10-11 AGAGTAC 8840 0.0 21.70508 10-11 GAGTACT 4650 0.0 21.559113 12-13 >>END_MODULE