FastQCFastQC Report
Thu 26 May 2016
SRR1364458_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364458_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737476
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28950.049.9733851
ATCAACG32150.043.2883953
TCAACGC32550.042.576054
CAACGCA35300.039.2606475
AACGCAG35650.038.7083556
GTACATG94900.036.8886031
TATCAAC39200.036.565172
TACATGG98450.034.9783632
ACATGGG97750.034.2553333
GGTATCA25800.033.4602621
ACGCAGA43650.031.3415057
CGCAGAG45100.030.4657368
CATGGGG74650.028.7632394
CATGGGA33850.022.6668644
ATGGGGA42300.022.0767735
GAGTACA75700.021.3134861
AGTACAT70450.021.0210782
GCAGAGT63100.020.8327759
ATACCGT2607.727049E-720.5894816
TAAGGTG11200.019.1188055
Produced by FastQC (version 0.11.5)