FastQCFastQC Report
Thu 26 May 2016
SRR1364458_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364458_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737476
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41400.052.233451
GGTATCA33600.044.501731
ATCAACG50850.042.040823
TCAACGC51900.041.0755584
CAACGCA54600.039.233785
AACGCAG56100.038.1805656
TATCAAC57050.037.472662
GTACATG90800.034.5754051
ACATGGG92000.032.6867183
ACGCAGA66250.032.1513987
TACATGG96950.031.6940382
CGCAGAG70550.030.0220138
CGTATAG2401.2732926E-1129.7860181
CATGGGG69750.027.9171644
TCCGTTG3850.024.7255578
GCAGAGT89050.023.3173059
CCGTTGC4400.022.9870439
TATTCCG5050.022.3878575
ACGTATA1602.6131215E-422.3117687
CATGGGA35450.022.173054
Produced by FastQC (version 0.11.5)