FastQCFastQC Report
Thu 26 May 2016
SRR1364457_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364457_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233374
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.050.249141
GTATCAA31550.043.018721
GTACATG97200.039.8078881
TCAACGC33950.038.3677564
CAACGCA34050.038.0821045
ATCAACG34500.037.7552533
TACATGG102450.037.0391582
ACATGGG101450.036.0558243
AACGCAG37450.034.465896
TATCAAC40450.032.4957162
CATGGGA64900.030.060164
CATGGGG45500.029.6740324
ACGCAGA45850.028.2812587
CGCAGAG47450.026.8261978
GAGTACA70950.024.9187411
ATGGGAT31350.024.0961535
AGTACAT71550.023.2755932
ATGGGAG29800.022.3554445
ATGGGGA28750.020.8960915
GCAGAGT58450.020.7604589
Produced by FastQC (version 0.11.5)