##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364457_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2233374 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.707717560963815 31.0 31.0 34.0 27.0 34.0 2 30.712731051762937 31.0 31.0 34.0 27.0 34.0 3 30.6300816611996 31.0 31.0 34.0 26.0 34.0 4 34.18973893311196 37.0 35.0 37.0 30.0 37.0 5 34.35562382296919 37.0 35.0 37.0 30.0 37.0 6 34.45558961463687 37.0 35.0 37.0 31.0 37.0 7 34.315146052564415 37.0 35.0 37.0 30.0 37.0 8 34.17084017276103 37.0 35.0 37.0 30.0 37.0 9 35.250194996449316 39.0 35.0 39.0 28.0 39.0 10-11 35.46624971903496 39.0 35.0 39.0 29.0 39.0 12-13 35.54909656868935 39.0 35.0 39.0 30.0 39.0 14-15 36.705832968414605 40.0 36.0 41.0 30.0 41.0 16-17 36.862981748690544 40.0 37.0 41.0 30.0 41.0 18-19 36.85038332137833 40.0 36.5 41.0 30.0 41.0 20-21 36.80383178097354 40.0 37.0 41.0 30.0 41.0 22-23 36.72164782969624 40.0 36.5 41.0 29.5 41.0 24-25 36.78397236647332 40.0 36.0 41.0 30.0 41.0 26-27 36.80199778451795 40.0 36.0 41.0 30.0 41.0 28-29 36.7550811015083 40.0 36.0 41.0 30.0 41.0 30-31 36.53585472025733 40.0 36.0 41.0 29.5 41.0 32-33 36.612525040588814 40.0 36.0 41.0 30.0 41.0 34-35 36.5632001178486 40.0 36.0 41.0 30.0 41.0 36-37 36.45035090405817 40.0 36.0 41.0 29.5 41.0 38-39 36.31188864919176 40.0 35.5 41.0 29.0 41.0 40-41 36.28240254431188 40.0 35.0 41.0 29.0 41.0 42-43 36.15536470828442 40.0 35.0 41.0 28.5 41.0 44-45 36.13642430690068 39.5 35.0 41.0 29.0 41.0 46-47 35.99692057846111 39.0 35.0 41.0 28.0 41.0 48-49 35.83189873259024 39.0 35.0 41.0 28.0 41.0 50-51 35.70288003710977 39.0 35.0 41.0 27.5 41.0 52-53 35.57037558420578 39.0 35.0 41.0 28.0 41.0 54-55 35.44553576785617 39.0 34.0 40.0 28.0 41.0 56-57 35.39690642946502 38.5 34.0 40.0 28.0 41.0 58-59 35.367180776708246 38.5 34.0 41.0 28.0 41.0 60-61 35.1765611133648 38.0 34.0 40.0 28.0 41.0 62-63 34.916243987796044 37.5 34.0 40.0 27.5 41.0 64-65 34.61435344013139 37.0 34.0 40.0 27.0 41.0 66-67 34.31248192197097 37.0 34.0 40.0 26.0 41.0 68-69 34.00927475648951 36.0 33.0 39.0 26.0 41.0 70-71 33.66580966734635 36.0 33.0 39.0 26.0 41.0 72-73 33.33275550803403 35.0 33.0 38.5 26.0 40.0 74-75 32.97416464953922 35.0 33.0 37.0 26.0 39.5 76-77 32.62929585461279 35.0 33.0 37.0 26.0 39.0 78-79 32.31580715992933 35.0 32.0 36.5 25.0 39.0 80-81 31.998040408816436 35.0 32.0 36.0 25.0 38.0 82-83 31.715981962716498 35.0 32.0 36.0 24.0 37.0 84-85 31.38076963374697 35.0 32.0 35.0 23.0 37.0 86-87 31.191145773166518 35.0 32.0 35.0 23.0 36.0 88-89 31.056305616524597 35.0 32.0 35.0 23.0 36.0 90-91 30.93215601148755 35.0 32.0 35.0 22.5 36.0 92-93 30.705730656844757 35.0 31.0 35.0 20.0 35.5 94-95 30.634378075503697 34.5 31.0 35.0 20.0 35.0 96-97 30.557838722936687 34.0 31.0 35.0 20.0 35.0 98-99 30.411551312050737 34.0 31.0 35.0 18.5 35.0 100-101 30.28679455389021 34.0 31.0 35.0 18.5 35.0 102-103 29.258329325943617 33.5 29.5 34.5 17.0 35.0 104-105 30.20904089507624 34.0 31.0 35.0 18.5 35.0 106-107 30.45564983742087 35.0 32.0 35.0 19.0 35.0 108-109 30.450481647946113 35.0 32.0 35.0 18.0 35.0 110-111 30.34432522273475 35.0 32.0 35.0 17.0 35.0 112-113 30.231500859238082 35.0 31.5 35.0 12.5 35.0 114-115 30.135616560414867 35.0 31.0 35.0 8.5 35.0 116-117 30.017611470358304 35.0 31.0 35.0 2.0 35.0 118-119 29.762379699951733 35.0 31.0 35.0 2.0 35.0 120-121 29.637693462895154 35.0 31.0 35.0 2.0 35.0 122-123 29.41388343376434 35.0 31.0 35.0 2.0 35.0 124-125 28.243922424099143 33.5 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6844978842841805 1101 2 0.6510306042821625 1101 3 0.8226323101065418 1101 4 0.6506164045164837 1101 5 0.5755593379339388 1101 6 0.5267269874627232 1101 7 0.657323412440995 1101 8 0.7005256621820592 1101 9 1.2233403333289345 1101 10-11 1.0181371639186736 1101 12-13 0.89502291970512 1101 14-15 1.1418001363829973 1101 16-17 0.834151738533329 1101 18-19 0.9279058688033288 1101 20-21 1.0691086456718466 1101 22-23 0.9646898209068411 1101 24-25 0.9712589185029401 1101 26-27 0.96998619179044 1101 28-29 0.7973081401576252 1101 30-31 0.9844178139826667 1101 32-33 0.8399480560011341 1101 34-35 0.8337488705526539 1101 36-37 0.7562581404119442 1101 38-39 0.8611829516026432 1101 40-41 0.7680250891554792 1101 42-43 0.8428659569766168 1101 44-45 0.6724871018658263 1101 46-47 0.7634271694735233 1101 48-49 0.6704365815348794 1101 50-51 0.6149205665274238 1101 52-53 0.49700049374214217 1101 54-55 0.530686753017136 1101 56-57 0.5319386849528911 1101 58-59 0.6559163942685089 1101 60-61 0.585114096738657 1101 62-63 0.6990775801760947 1101 64-65 0.4948444690993625 1101 66-67 0.5862764514264285 1101 68-69 0.4861274154434696 1101 70-71 0.39680943815632475 1101 72-73 0.5634560770714003 1101 74-75 0.5612072081312505 1101 76-77 0.5651077730767895 1101 78-79 0.425731206902384 1101 80-81 0.5462398863332503 1101 82-83 0.5442745565435132 1101 84-85 0.6817030902481775 1101 86-87 0.6264578977694804 1101 88-89 0.6202437272182486 1101 90-91 0.6175082863609305 1101 92-93 0.6986331181120384 1101 94-95 0.5604185361072851 1101 96-97 0.557253832475908 1101 98-99 0.5914539570471185 1101 100-101 0.5112565182244211 1101 102-103 0.478226963818706 1101 104-105 0.5364864426880445 1101 106-107 0.45126345862069783 1101 108-109 0.45831185404535546 1101 110-111 0.5214636446258609 1101 112-113 0.39913580661718484 1101 114-115 0.4212981865337717 1101 116-117 0.4729912696024847 1101 118-119 0.42053702851353236 1101 120-121 0.24878736429325343 1101 122-123 0.40129097253780444 1101 124-125 0.08636199032362768 1102 1 0.41171246128242345 1102 2 0.35227983701603094 1102 3 0.4046400956530114 1102 4 0.40778928456897745 1102 5 0.3304560833144734 1102 6 0.2960388114748014 1102 7 0.35154113611230287 1102 8 0.5853155370912191 1102 9 0.8193046898484013 1102 10-11 0.5283652183434171 1102 12-13 0.5060616745821136 1102 14-15 0.6606716642832637 1102 16-17 0.5609076314900037 1102 18-19 0.4457549925324855 1102 20-21 0.6023555219285726 1102 22-23 0.5207958041716196 1102 24-25 0.4916814704830159 1102 26-27 0.5437682229467384 1102 28-29 0.5077982556532206 1102 30-31 0.549477043400536 1102 32-33 0.38807707312439277 1102 34-35 0.31642307524162305 1102 36-37 0.2986414365872534 1102 38-39 0.49110963800068674 1102 40-41 0.4503771743555305 1102 42-43 0.5191436755680954 1102 44-45 0.39938903996244335 1102 46-47 0.3822923475850999 1102 48-49 0.36079429511707417 1102 50-51 0.3080493455336111 1102 52-53 0.24778985184905622 1102 54-55 0.1683525530552359 1102 56-57 0.30202884313318634 1102 58-59 0.40576285285025193 1102 60-61 0.27088095719147276 1102 62-63 0.30248821042789586 1102 64-65 0.27235501680390684 1102 66-67 0.30783834951937195 1102 68-69 0.3172126296077309 1102 70-71 0.3374555379750035 1102 72-73 0.3132408897879131 1102 74-75 0.3178441483797343 1102 76-77 0.29591610999657547 1102 78-79 0.2636631248207806 1102 80-81 0.20276808645037292 1102 82-83 0.2386409270042904 1102 84-85 0.42575148622099945 1102 86-87 0.32669350138744946 1102 88-89 0.2793911269797782 1102 90-91 0.15463166451906574 1102 92-93 0.22486047935151632 1102 94-95 0.2997327236518217 1102 96-97 0.2523500317746077 1102 98-99 0.23704438156577012 1102 100-101 0.18583483052114147 1102 102-103 0.14128067421189883 1102 104-105 0.2284633082912393 1102 106-107 0.1740219391162654 1102 108-109 0.16893302212426065 1102 110-111 0.17952778807463687 1102 112-113 0.06289518137466032 1102 114-115 -0.008816259588272146 1102 116-117 -0.0318257947490963 1102 118-119 0.07739003796060828 1102 120-121 -0.030668291251874535 1102 122-123 -0.05674358265949664 1102 124-125 -0.33559478525367226 1103 1 0.5396685220160471 1103 2 0.5511684951838944 1103 3 0.6696932278852508 1103 4 0.6127267678400372 1103 5 0.5185928654832068 1103 6 0.3977413440200408 1103 7 0.5404318673400113 1103 8 0.6513452365455521 1103 9 1.0652259146032392 1103 10-11 0.8333562170798601 1103 12-13 0.6537669739506455 1103 14-15 0.952766438955031 1103 16-17 0.8104077565766161 1103 18-19 0.7737329069862753 1103 20-21 0.6685988288516853 1103 22-23 0.7192094075594326 1103 24-25 0.6218772588964754 1103 26-27 0.68200140497013 1103 28-29 0.5871842732846986 1103 30-31 0.8795347049856019 1103 32-33 0.8027885362879772 1103 34-35 0.7519809212415609 1103 36-37 0.6811713034810367 1103 38-39 0.7200098668946211 1103 40-41 0.7211842907978792 1103 42-43 0.8447754658464532 1103 44-45 0.6656109904253995 1103 46-47 0.5919676042184179 1103 48-49 0.63540425036539 1103 50-51 0.5560493743403967 1103 52-53 0.4613992496517554 1103 54-55 0.41601808876973934 1103 56-57 0.4465414760237749 1103 58-59 0.558422105084361 1103 60-61 0.5499840853170284 1103 62-63 0.5780550911278439 1103 64-65 0.6285518367746974 1103 66-67 0.46241436762923627 1103 68-69 0.4585315243393282 1103 70-71 0.587638066693053 1103 72-73 0.4577626105338908 1103 74-75 0.6102616705193782 1103 76-77 0.48277832044764324 1103 78-79 0.4646608084464674 1103 80-81 0.5226406627150517 1103 82-83 0.4415354432380312 1103 84-85 0.5924453878756566 1103 86-87 0.5903403308661872 1103 88-89 0.5224055240288656 1103 90-91 0.41906746559379826 1103 92-93 0.4298056787262077 1103 94-95 0.4080579450739883 1103 96-97 0.4217896660381548 1103 98-99 0.3011428260164237 1103 100-101 0.2903584562873682 1103 102-103 0.3268694703608439 1103 104-105 0.2783499758758339 1103 106-107 0.2447224961675083 1103 108-109 0.2647395941526334 1103 110-111 0.35350294805090243 1103 112-113 0.3804366798659906 1103 114-115 0.3480324854046195 1103 116-117 0.25166543864263247 1103 118-119 0.08344889399851851 1103 120-121 0.1657724330399084 1103 122-123 0.3411718947984177 1103 124-125 1.6824908864521149E-4 1104 1 0.5109036681782904 1104 2 0.5388301066474632 1104 3 0.6591609326345811 1104 4 0.42258818815120236 1104 5 0.4113549049576193 1104 6 0.30520479868919637 1104 7 0.4690023834498547 1104 8 0.6055375910796812 1104 9 1.0196910122009584 1104 10-11 0.8280691657890671 1104 12-13 0.719022701264656 1104 14-15 0.7738355834692001 1104 16-17 0.5916507316592856 1104 18-19 0.6032200903019671 1104 20-21 0.7573160173551301 1104 22-23 0.7724947586854327 1104 24-25 0.8353313059207679 1104 26-27 0.7641737553600265 1104 28-29 0.7115387689248678 1104 30-31 0.7923417420884462 1104 32-33 0.7817999704270733 1104 34-35 0.773427003637515 1104 36-37 0.6389242490314686 1104 38-39 0.7705738013360772 1104 40-41 0.7001282442550334 1104 42-43 0.6666334748785019 1104 44-45 0.6713955821345081 1104 46-47 0.6736035516214756 1104 48-49 0.6331955956174582 1104 50-51 0.6708110783607495 1104 52-53 0.5599526911167629 1104 54-55 0.6211230707934163 1104 56-57 0.5451341533370453 1104 58-59 0.5677697763413718 1104 60-61 0.7341934896106679 1104 62-63 0.7608911992568608 1104 64-65 0.5910631450770509 1104 66-67 0.626020969935837 1104 68-69 0.5871337775222045 1104 70-71 0.6790555608379591 1104 72-73 0.7138745270997333 1104 74-75 0.6414912490069256 1104 76-77 0.6107830357173256 1104 78-79 0.4874473383898916 1104 80-81 0.6055074793240429 1104 82-83 0.609168655578955 1104 84-85 0.5780219416608716 1104 86-87 0.6125990017812768 1104 88-89 0.5838029075203792 1104 90-91 0.528218567739561 1104 92-93 0.5773661463113378 1104 94-95 0.5350526483174249 1104 96-97 0.5518723466711251 1104 98-99 0.602549709062572 1104 100-101 0.6205295295628481 1104 102-103 0.46924918843012264 1104 104-105 0.46373911005098734 1104 106-107 0.37249992555085853 1104 108-109 0.33861372853765204 1104 110-111 0.3669926972085733 1104 112-113 0.398696280496484 1104 114-115 0.3661381703555229 1104 116-117 0.39137521115600293 1104 118-119 0.36752340328912325 1104 120-121 0.23104773680251967 1104 122-123 0.2663110161576334 1104 124-125 0.09101246850347167 1105 1 0.316192010411914 1105 2 0.3296584858534821 1105 3 0.3132126745482111 1105 4 0.22871590511844175 1105 5 0.1572673981589645 1105 6 0.14251524863600906 1105 7 0.1424103983657048 1105 8 0.4303418435705595 1105 9 0.8482805096045496 1105 10-11 0.4622394178982461 1105 12-13 0.4623955806346558 1105 14-15 0.046371170553122454 1105 16-17 0.2164231078649479 1105 18-19 0.24370202596508506 1105 20-21 0.5224173435211554 1105 22-23 0.5582740980430287 1105 24-25 0.5737890718894789 1105 26-27 0.519795852021403 1105 28-29 0.5929798379623534 1105 30-31 0.7277056664224304 1105 32-33 0.612515534189626 1105 34-35 0.5106850598185702 1105 36-37 0.5879458568549154 1105 38-39 0.5757393456812068 1105 40-41 0.5556283872634111 1105 42-43 0.6469484616956294 1105 44-45 0.5545407277037384 1105 46-47 0.61655574589183 1105 48-49 0.5388114756357325 1105 50-51 0.47198338886953906 1105 52-53 0.3400443136913154 1105 54-55 0.31406746228127247 1105 56-57 0.41910770369517536 1105 58-59 0.523064437549678 1105 60-61 0.4576001997989536 1105 62-63 0.4519084313822219 1105 64-65 0.43826330205369146 1105 66-67 0.27342614822046585 1105 68-69 0.40036880602922054 1105 70-71 0.4331868143843849 1105 72-73 0.3193158294532026 1105 74-75 0.38112442101453325 1105 76-77 0.36405444821539135 1105 78-79 0.32397597511331355 1105 80-81 0.2538674111916812 1105 82-83 0.2993043011965284 1105 84-85 0.42025019121278717 1105 86-87 0.3716971740882009 1105 88-89 0.26639159957448655 1105 90-91 0.22001428731216421 1105 92-93 0.283865761800449 1105 94-95 0.2847170007555526 1105 96-97 0.3047575853927782 1105 98-99 0.17053125970314298 1105 100-101 0.03058301827644172 1105 102-103 0.05121255781998357 1105 104-105 0.09438800479324527 1105 106-107 0.03999458256356547 1105 108-109 0.09975315991179556 1105 110-111 0.14967944849322024 1105 112-113 0.15805232836924077 1105 114-115 0.1176451306933366 1105 116-117 0.07126260549225805 1105 118-119 -0.04696679871852183 1105 120-121 -0.13017261326356433 1105 122-123 -0.06491633338808711 1105 124-125 -0.3341510445599205 1106 1 0.4007153616200583 1106 2 0.34526458502818613 1106 3 0.5603498295429574 1106 4 0.26931921683229376 1106 5 0.17632276098819233 1106 6 0.16357197328887452 1106 7 0.10048733789498243 1106 8 0.3751969271628326 1106 9 0.8097208099069988 1106 10-11 0.32488991448702365 1106 12-13 0.5023224509858224 1106 14-15 0.6911698562740938 1106 16-17 0.49374153897679207 1106 18-19 0.40644810265776954 1106 20-21 0.5400224378074654 1106 22-23 0.5399408453532217 1106 24-25 0.45583556252887547 1106 26-27 0.5076961682141388 1106 28-29 0.417895653138622 1106 30-31 0.5500483396780211 1106 32-33 0.602034769071949 1106 34-35 0.4685440865284036 1106 36-37 0.46115180147455703 1106 38-39 0.6260161983959804 1106 40-41 0.5171834292716753 1106 42-43 0.5135738073232474 1106 44-45 0.35263669910403905 1106 46-47 0.39845674510011975 1106 48-49 0.36940880219781747 1106 50-51 0.3252939631986749 1106 52-53 0.32834171157031733 1106 54-55 0.2224122434883853 1106 56-57 0.1612524111310023 1106 58-59 0.31875530358395565 1106 60-61 0.2679219919925231 1106 62-63 0.3580256982821268 1106 64-65 0.3035210387476823 1106 66-67 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2216 72-73 -1.1193144888322593 2216 74-75 -0.9538371706643005 2216 76-77 -0.8971857536722112 2216 78-79 -0.8755510162482807 2216 80-81 -0.8902828919306778 2216 82-83 -1.1582133618310877 2216 84-85 -1.2509585025196266 2216 86-87 -1.245776364042385 2216 88-89 -1.5871106846722434 2216 90-91 -1.3056310693076476 2216 92-93 -1.124442128455101 2216 94-95 -1.0498446771938497 2216 96-97 -0.9278116665470364 2216 98-99 -0.8894552654521739 2216 100-101 -1.055721126245171 2216 102-103 -1.3091977419127048 2216 104-105 -1.130149684085957 2216 106-107 -0.8654293753058866 2216 108-109 -0.8462705280004776 2216 110-111 -0.8560757995371304 2216 112-113 -1.034605977219588 2216 114-115 -0.926037070757971 2216 116-117 -0.8473046685369638 2216 118-119 -0.6407535752881053 2216 120-121 -0.540640473284963 2216 122-123 -0.529373368820341 2216 124-125 -0.4518568211536724 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35819.0 3 6811.0 4 4432.0 5 3920.0 6 4259.0 7 5058.0 8 5593.0 9 6304.0 10 6880.0 11 7547.0 12 7899.0 13 8315.0 14 8579.0 15 8366.0 16 7871.0 17 7406.0 18 6938.0 19 6783.0 20 6656.0 21 7125.0 22 8621.0 23 10974.0 24 16548.0 25 23400.0 26 27543.0 27 30257.0 28 36091.0 29 43212.0 30 52331.0 31 63655.0 32 80766.0 33 105121.0 34 144470.0 35 211237.0 36 378726.0 37 610736.0 38 226617.0 39 508.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.63938408037787 18.412071020766675 12.349672411784212 24.598872487071244 2 15.699972283999896 20.086712125682883 39.31933264886604 24.89398294145118 3 18.603377669839443 24.984619683044578 29.30588428091309 27.106118366202885 4 12.584946136414416 16.060209722982798 36.98589593015264 34.36894821045015 5 13.462049078848096 38.6633054901952 34.33070125753074 13.54394417342596 6 32.73007404064175 36.51910477807508 17.317656561748894 13.433164619534264 7 29.041991642237996 32.48436333742729 20.97186632058326 17.50177869975145 8 26.622329624550055 35.53384225559217 19.861714279061875 17.9821138407959 9 26.825721199052193 15.96587031939263 19.619014186670867 37.58939429488431 10-11 24.87764510800972 26.804134329203677 27.730805132020585 20.58741543076602 12-13 25.331450047362004 24.135903754504763 27.724982934679844 22.807663263453392 14-15 22.65389238609683 25.589203728401532 24.75355299883871 27.003350886662925 16-17 22.936301112245445 28.933717514731434 25.540294270413554 22.589687102609567 18-19 23.09063148426915 27.806785208650865 26.699621615921014 22.40296169115897 20-21 23.573093868367714 27.06873959349353 26.74341171296806 22.614754825170692 22-23 23.506902105940508 26.946827341895496 26.57748507831283 22.968785473851167 24-25 23.250591323552726 27.18100098247927 26.61303034401104 22.955377349956965 26-27 23.048734069516467 27.502753969746635 26.28888023142302 23.159631729313883 28-29 23.063928662265226 27.39464931166286 26.51495068977005 23.026471336301856 30-31 23.15713388763445 27.098266786447777 26.5615805374207 23.183018788497073 32-33 22.855647329509292 27.268528479167053 26.57149618360602 23.30432800771764 34-35 23.364507741512856 27.22881645457114 26.346061453001745 23.060614350914253 36-37 23.49689683226596 26.928067671936894 26.306852043308677 23.268183452488465 38-39 23.099439948991837 27.084273617245348 26.493439504819627 23.322846928943193 40-41 23.224900390512623 26.807371807285612 26.786886853428253 23.18084094877351 42-43 23.129814452912925 26.851390612473075 26.506768303344646 23.512026631269354 44-45 23.01021422469145 27.007897531786245 26.591640562453993 23.39024768106831 46-47 23.36216683580799 27.227118850492726 26.228009668928525 23.18270464477076 48-49 23.157258169241008 27.148962427805195 26.270937500279867 23.422841902673927 50-51 23.152863653796253 27.099333288406775 26.44477873185764 23.303024325939333 52-53 23.334169206003057 26.883881613562632 26.440592962101384 23.341356218332926 54-55 23.11515406348731 26.88322269546229 26.49001773922599 23.511605501824413 56-57 23.064579362394355 27.202677471725494 26.468154981872033 23.26458818400812 58-59 23.100966043288466 26.834451207839578 26.439834827576707 23.624747921295246 60-61 22.988097066606006 26.997976398329353 26.6632469783673 23.350679556697344 62-63 22.996924407966446 26.966869765604777 26.783668731689975 23.252537094738805 64-65 22.906387066006623 26.921146255236355 26.595803751144665 23.576662927612357 66-67 22.836373813988637 27.09019563939657 26.489092893727783 23.58433765288701 68-69 22.812251044855923 26.749296824166407 26.920517508753594 23.517934622224075 70-71 22.891936082225413 26.669011503506844 26.737901347146824 23.701151067120918 72-73 22.839387723618067 26.83291414333544 26.567674685573916 23.760023447472577 74-75 22.823615708039995 26.971285757339775 26.5446091551866 23.66048937943363 76-77 23.01771156361881 26.800323383970102 26.62902659947337 23.552938452937713 78-79 22.90472799016479 26.695453453462864 26.80457934699549 23.595239209376857 80-81 22.991377953381793 26.975987375140193 26.612113587017244 23.42052108446077 82-83 22.90311977157024 26.820590743149076 26.690585333347666 23.58570415193302 84-85 22.839747218181948 26.79611315712167 26.72103720793597 23.64310241676041 86-87 22.9368129044595 26.730304651308074 26.860869177748008 23.472013266484414 88-89 22.900380139933233 26.66785639994026 26.770431709720604 23.6613317504059 90-91 22.880991233695283 26.77090460956448 26.77180022712861 23.576303929611626 92-93 22.901890015706286 26.49456549117683 26.837237888081244 23.766306605035638 94-95 22.766745268444623 26.388479660110747 26.981105123683612 23.86366994776101 96-97 22.91681873077555 26.326181879570598 26.932788932628622 23.824210457025227 98-99 22.831749196065168 26.318955756773416 26.838426506486446 24.01086854067497 100-101 22.555263070103145 26.300562051210402 27.153591133996475 23.990583744689978 102-103 22.627796061874907 26.107951727595292 27.16850897075099 24.095743239778812 104-105 22.49880716725116 26.318334874891942 27.103869256126885 24.078988701730015 106-107 22.55598427590864 26.252551761751093 26.971276532389183 24.22018742995109 108-109 22.645367841311064 26.2130703443335 26.8611024045135 24.28045940984194 110-111 22.544372537890872 26.469350904354584 26.987413927631483 23.998862630123064 112-113 22.458534942730655 26.45273512986328 27.26550152462267 23.823228402783393 114-115 22.381696713953723 26.551931908788085 26.96445936266479 24.101912014593406 116-117 22.235596568226406 26.520790764178187 27.112001042482504 24.131611625112903 118-119 22.1812132110841 26.49504208534187 26.99708212783668 24.32666257573735 120-121 22.15297701636416 26.62880250232219 27.10289205600487 24.115328425308782 122-123 22.168932792392248 26.668511903682212 27.105389682034758 24.057165621890782 124-125 22.23517808514291 26.641353364054886 27.000766355748397 24.122702195053808 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 504.0 1 435.5 2 308.0 3 236.5 4 202.5 5 164.5 6 156.0 7 165.0 8 165.5 9 171.0 10 173.5 11 163.5 12 160.0 13 168.5 14 199.5 15 242.5 16 277.0 17 346.5 18 466.5 19 572.0 20 722.0 21 1050.5 22 1579.5 23 2284.5 24 3161.0 25 4579.0 26 6737.0 27 9652.5 28 12653.5 29 15935.0 30 20695.5 31 25940.5 32 31715.5 33 36417.5 34 40265.5 35 45251.0 36 52188.0 37 58468.5 38 61914.5 39 64543.0 40 66424.0 41 69887.0 42 72764.5 43 75858.0 44 80840.0 45 84421.0 46 85366.0 47 85955.0 48 86179.5 49 84862.5 50 83795.0 51 81628.5 52 81201.5 53 82448.0 54 82047.0 55 77614.5 56 70831.0 57 65043.0 58 60122.5 59 53259.5 60 43822.0 61 36146.5 62 30237.5 63 23568.5 64 17649.5 65 13513.0 66 9823.0 67 7673.5 68 5830.5 69 3921.0 70 2795.0 71 1974.0 72 1443.5 73 1019.5 74 765.5 75 647.0 76 459.5 77 273.0 78 163.0 79 85.5 80 55.5 81 37.0 82 26.0 83 21.0 84 16.0 85 12.5 86 11.0 87 8.5 88 7.5 89 6.5 90 5.0 91 3.5 92 5.5 93 5.5 94 3.0 95 3.0 96 3.0 97 2.0 98 0.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08569993203108839 2 3.1342712864034416E-4 3 0.0 4 0.002104439292299454 5 0.0012984838186528545 6 2.6865182454886643E-4 7 4.477530409147774E-5 8 0.0012089332104698988 9 4.477530409147774E-4 10-11 0.009783403943987886 12-13 0.0019477257279792817 14-15 0.003783513195729869 16-17 0.0024178664209397976 18-19 0.014574361481776004 20-21 0.017731020420225185 22-23 0.009626690379667714 24-25 0.011104275414686478 26-27 0.01047742115740579 28-29 0.007432700479185304 30-31 0.018447425285688827 32-33 0.0021716022484366704 34-35 0.0011193826022869433 36-37 4.925283450062551E-4 38-39 2.6865182454886643E-4 40-41 0.0012313208625156378 42-43 0.0026641305934429252 44-45 8.059554736465994E-4 46-47 0.001544747991155982 48-49 0.008059554736465992 50-51 0.004455142757102035 52-53 0.008014779432374516 54-55 0.008395369517152076 56-57 0.009962505160353796 58-59 0.008619246037609465 60-61 0.010163994028765447 62-63 0.013790793660175145 64-65 0.008462532473289293 66-67 0.013947507224495314 68-69 0.013029613490620021 70-71 0.004410367453010557 72-73 0.013320652967214625 74-75 0.013477366531534798 76-77 0.005171547622565678 78-79 0.007768515259871387 80-81 0.014305709657227137 82-83 0.017462368595676316 84-85 0.013522141835626276 86-87 0.016947452598624325 88-89 0.017037003206807278 90-91 0.012447534537430811 92-93 0.008574470733517987 94-95 0.007880453520100082 96-97 0.009626690379667714 98-99 0.006246154920761145 100-101 0.009604302727621975 102-103 0.011440090195372562 104-105 0.010947561850366307 106-107 0.005059609362336984 108-109 0.0033133725027693524 110-111 0.006783458569858878 112-113 0.00888789786215833 114-115 0.013902731920403838 116-117 0.01088039889422909 118-119 0.21789901736117642 120-121 0.025432372723959356 122-123 0.026798019498749426 124-125 0.23715239812051184 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2233374.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.32628049490643 #Duplication Level Percentage of deduplicated Percentage of total 1 86.13609180874927 60.57630953277112 2 8.47821569531792 11.924827501704922 3 2.422530818707957 5.111027455920333 4 1.040886971571583 2.928068365049473 5 0.5054404978527699 1.7772875112739523 6 0.31342649913542847 1.3225271935640812 7 0.20809925188659506 1.0244392451269808 8 0.14306670100726981 0.8049079155614534 9 0.10330187411890882 0.6538352917442304 >10 0.5652587468295419 7.498603149213726 >50 0.055404933582911174 2.6780032738526747 >100 0.027104131186910553 3.183241187842982 >500 0.0011720700530008046 0.5169223763741142 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 4.477530409147774E-5 0.0 0.0 0.0 0.0 20-21 4.477530409147774E-5 0.0 0.0 4.477530409147774E-5 0.0 22-23 4.477530409147774E-5 0.0 0.0 6.716295613721661E-5 0.0 24-25 4.477530409147774E-5 0.0 0.0 1.1193826022869434E-4 0.0 26-27 4.477530409147774E-5 0.0 0.0 4.7014069296051625E-4 0.0 28-29 4.477530409147774E-5 0.0 0.0 0.0024402540729855367 0.0 30-31 4.477530409147774E-5 0.0 0.0 0.009872954552170842 0.0 32-33 4.477530409147774E-5 0.0 0.0 0.025253271507593444 0.0 34-35 4.477530409147774E-5 0.0 0.0 0.05301396004430964 0.0 36-37 4.477530409147774E-5 0.0 0.0 0.09973698986376667 0.0 38-39 4.477530409147774E-5 0.0 0.0 0.16510893383732417 0.0 40-41 4.477530409147774E-5 0.0 0.0 0.23128683328452826 0.0 42-43 4.477530409147774E-5 0.0 0.0 0.29992737445676365 0.0 44-45 4.477530409147774E-5 0.0 0.0 0.3853138793592117 0.0 46-47 4.477530409147774E-5 0.0 0.0 0.49324474987171874 0.0 48-49 4.477530409147774E-5 0.0 0.0 0.6083396690388623 0.0 50-51 4.477530409147774E-5 0.0 0.0 0.7273076520099186 0.0 52-53 4.477530409147774E-5 0.0 0.0 0.8422010823086505 0.0 54-55 4.477530409147774E-5 0.0 0.0 0.968445052194572 0.0 56-57 4.477530409147774E-5 0.0 0.0 1.1146811953573383 0.0 58-59 4.477530409147774E-5 0.0 0.0 1.2831258893494777 0.0 60-61 4.477530409147774E-5 0.0 0.0 1.465249438741563 0.0 62-63 4.477530409147774E-5 0.0 0.0 1.6439029020665594 0.0 64-65 4.477530409147774E-5 0.0 0.0 1.8312875496893937 0.0 66-67 4.477530409147774E-5 0.0 0.0 2.0457388686355262 0.0 68-69 4.477530409147774E-5 0.0 0.0 2.2886896686358846 0.0 70-71 4.477530409147774E-5 0.0 0.0 2.55519227858836 0.0 72-73 4.477530409147774E-5 0.0 0.0 2.824806772175193 0.0 74-75 4.477530409147774E-5 0.0 0.0 3.0895855329201467 0.0 76-77 4.477530409147774E-5 0.0 0.0 3.3790354862195047 0.0 78-79 4.477530409147774E-5 0.0 0.0 3.7094772304146106 0.0 80-81 4.477530409147774E-5 0.0 0.0 4.070410956696012 0.0 82-83 8.955060818295548E-5 0.0 0.0 4.448762276268999 0.0 84-85 8.955060818295548E-5 0.0 0.0 4.824382302292406 0.0 86-87 8.955060818295548E-5 0.0 0.0 5.216166213092836 0.0 88-89 8.955060818295548E-5 0.0 0.0 5.639852528058444 2.238765204573887E-5 90-91 8.955060818295548E-5 0.0 0.0 6.0960904890985566 4.477530409147774E-5 92-93 8.955060818295548E-5 0.0 0.0 6.576036973655107 4.477530409147774E-5 94-95 8.955060818295548E-5 0.0 0.0 7.065610148591324 4.477530409147774E-5 96-97 8.955060818295548E-5 0.0 0.0 7.5611832142758 8.955060818295548E-5 98-99 8.955060818295548E-5 0.0 0.0 8.079076769049877 8.955060818295548E-5 100-101 1.1193826022869434E-4 0.0 0.0 8.656611028873803 8.955060818295548E-5 102-103 1.3432591227443321E-4 0.0 0.0 9.270681936836374 1.1193826022869434E-4 104-105 1.3432591227443321E-4 0.0 0.0 9.882693180810737 1.3432591227443321E-4 106-107 1.3432591227443321E-4 0.0 0.0 10.485189672665662 1.3432591227443321E-4 108-109 1.3432591227443321E-4 0.0 0.0 11.11493193706025 1.3432591227443321E-4 110-111 1.3432591227443321E-4 0.0 0.0 11.784994362789215 1.3432591227443321E-4 112-113 1.3432591227443321E-4 0.0 0.0 12.49992164321784 1.3432591227443321E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2085 0.0 50.24914 1 GTATCAA 3155 0.0 43.01872 1 GTACATG 9720 0.0 39.807888 1 TCAACGC 3395 0.0 38.367756 4 CAACGCA 3405 0.0 38.082104 5 ATCAACG 3450 0.0 37.755253 3 TACATGG 10245 0.0 37.039158 2 ACATGGG 10145 0.0 36.055824 3 AACGCAG 3745 0.0 34.46589 6 TATCAAC 4045 0.0 32.495716 2 CATGGGA 6490 0.0 30.06016 4 CATGGGG 4550 0.0 29.674032 4 ACGCAGA 4585 0.0 28.281258 7 CGCAGAG 4745 0.0 26.826197 8 GAGTACA 7095 0.0 24.918741 1 ATGGGAT 3135 0.0 24.096153 5 AGTACAT 7155 0.0 23.275593 2 ATGGGAG 2980 0.0 22.355444 5 ATGGGGA 2875 0.0 20.896091 5 GCAGAGT 5845 0.0 20.760458 9 >>END_MODULE