FastQCFastQC Report
Thu 26 May 2016
SRR1364457_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364457_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233374
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28910.12944540412846214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27420.12277388381883195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31350.068.02311
GTATCAA55050.051.722741
ATCAACG71400.039.443843
TCAACGC72150.039.198874
CAACGCA73100.038.58125
AACGCAG75900.037.1579136
TATCAAC76450.036.9186942
GTACATG101850.035.032951
ACATGGG104700.032.7560923
TACATGG108100.032.6643872
ACGCAGA91850.030.7701237
CGCAGAG93750.030.272098
GTGGTAT18150.030.1941011
CATGGGA68350.028.1371614
TGGTATC19000.026.9519582
GCAGAGT108100.025.9783529
CATGGGG48000.024.9328044
GAGTACT58550.023.57525412-13
CAGAGTA103200.023.5509110-11
AGAGTAC101400.023.52890610-11
Produced by FastQC (version 0.11.5)