FastQCFastQC Report
Thu 26 May 2016
SRR1364456_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364456_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2510883
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16800.051.013261
GTATCAA28450.046.8592871
CAACGCA30350.040.1774865
ATCAACG30900.039.262823
AACGCAG31350.038.7061776
TCAACGC31700.038.2734834
TATCAAC35800.035.2178342
GTACATG75700.031.9984111
ACGCAGA38350.031.1758547
TACATGG77950.030.5941242
CGCAGAG39700.029.9658878
ACATGGG77900.029.0868913
TAAGGTG17800.028.0702925
CATGGGG53500.024.6789264
AGGTAAG16500.024.5095182
GTAAGGT18250.024.4413664
GTGTAAG14450.023.476691
GGACCGA5950.021.993456
TAAGGTA10900.021.8253174
AAGGTAA19500.021.6697371
Produced by FastQC (version 0.11.5)