FastQCFastQC Report
Thu 26 May 2016
SRR1364456_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364456_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2510883
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27150.065.176231
GTATCAA52200.055.014751
ATCAACG64800.043.928633
CAACGCA66200.042.8737755
TCAACGC68350.041.647044
AACGCAG69600.040.948726
TATCAAC70850.040.1782952
ACGCAGA82350.034.464257
CGCAGAG82650.034.1204648
TAAGGTG16200.031.2201715
GTACATG75550.029.8098561
TACATGG77550.028.7973772
AGGTAAG15200.028.6010972
GTAAGGT16750.028.087114
ACATGGG76300.027.707583
GCAGAGT103300.026.7813269
AGAGTAC86900.026.22155610-11
GGTAAGG19550.024.0644043
CAGAGTA92250.023.8301910-11
TAAGGTA10500.023.2535554
Produced by FastQC (version 0.11.5)