FastQCFastQC Report
Thu 26 May 2016
SRR1364455_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364455_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2372581
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18650.047.2343141
GTACATG85150.039.354871
GTATCAA28800.037.6143571
TACATGG88400.036.735462
ATCAACG27600.035.9875683
ACATGGG89500.034.622613
TCAACGC28800.034.2830124
CAACGCA29000.034.0480165
AACGCAG30600.032.0726666
TATCAAC34600.029.9101282
TATTCCG3200.027.881945
CATGGGG56100.027.566014
CATGGGA44600.026.538824
GAGTACA61400.025.883181
ACGCAGA39800.024.807817
GTATAGG7150.024.1416361
CGCAGAG40750.023.9375488
AGTACAT64350.023.568762
ATTCCGT4100.023.2117946
TCCGTTG3609.458745E-1121.4785148
Produced by FastQC (version 0.11.5)