FastQCFastQC Report
Thu 26 May 2016
SRR1364455_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364455_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2372581
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24350.10263084800898263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31550.061.7445951
GTATCAA52700.047.4776541
ATCAACG61650.039.3090023
TCAACGC64200.037.747664
CAACGCA64700.037.3363575
AACGCAG65600.037.003186
GTACATG83150.035.894351
TATCAAC67950.035.665212
TACATGG86100.034.64772
ACATGGG91000.032.0616153
ACGCAGA79000.030.726697
CGCAGAG81350.029.8384468
CATGGGG51700.025.9132964
GCAGAGT96550.024.8944579
CATGGGA47050.024.424654
AGAGTAC85350.024.18797710-11
CAGAGTA90300.023.52090610-11
TGGTATC16350.022.9436572
GAGTACT56200.022.65432212-13
GTGGTAT17600.022.0014481
Produced by FastQC (version 0.11.5)