FastQCFastQC Report
Thu 26 May 2016
SRR1364454_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364454_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583818
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.061.5632061
GTATCAA34000.055.679871
TCAACGC39250.046.3681954
ATCAACG39300.046.0050773
CAACGCA41050.044.1956985
TATCAAC45100.042.0667422
AACGCAG43600.041.3379976
GTACATG115150.041.1010741
TACATGG116100.039.854022
ACATGGG119950.036.6411363
ACGCAGA53450.033.720057
CATGGGA88700.033.258034
CGCAGAG54700.033.0582288
CATGGGG33350.031.2090574
ATGGGAG35200.030.2484875
GCAGAGT60600.029.1525889
GTATAGG5050.028.2924041
GAGTACA80650.026.868721
AGTACAT80200.025.806532
ATGGGAT34750.025.162625
Produced by FastQC (version 0.11.5)